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The map label for this gene is aspS

Identifier: 15835443

GI number: 15835443

Start: 959949

End: 961697

Strand: Reverse

Name: aspS

Synonym: TC0829

Alternate gene names: 15835443

Gene position: 961697-959949 (Counterclockwise)

Preceding gene: 15835444

Following gene: 15835442

Centisome position: 89.63

GC content: 40.99

Gene sequence:

>1749_bases
ATGAAGTACAGAACGCATAAATGTAATGAGTTGTCCCTTAATCATGTTGGGGAACATGTTCGTTTGTCTGGGTGGGTGCA
TCGTTACCGTAACCATGGTGGAGTTGTTTTTATTGATTTGAGAGACCGATTTGGGATTACACAGATCGTCTGTCGTCAGG
AAGAGAACCCAGAACTTCATCGGCTTATGGATCAAGTCCGGTCTGAATGGGTGCTTTGTGTAGAAGGTCTTGTTTGCGCT
CGTTTAGGAGGAATGGAAAATCCCAATTTGGTTACAGGAAGTATTGAAGTTGAGGTTTCTCATTTAGAGGTGTTGTCTCG
AGCTCAAAATTTGCCATTTTCCATTTCTGATGAGCATATTAATGTAAATGAAGAGCTACGATTGACTTATCGCTATCTTG
ATATGCGGCGAGGGGATATTGTGGACAGATTGATGTGTCGGCATAAAGTTATGTTGGCTTGCAGACAATATCTAGATGAA
CAAGGTTTTACTGAGGTTGTTACCCCTGTTTTAGGGAAATCTACTCCTGAAGGAGCTCGGGATTACTTAGTGCCTTCGCG
GATATATCCAGGGAATTTCTATGCTCTTCCACAATCTCCACAGCTATTTAAACAGATTTTGATGGTTGGAGGGTTAGATC
GTTATTTCCAGATAGCAACTTGTTTCCGTGATGAAGATTTACGTGCGGATCGTCAACCTGAGTTTTCGCAAATTGATATG
GAAATGAGCTTTGGAGGACCGGAAGATCTCTTTCCAATAGTAGAGGAATTGGTCACGCGTTTATTTGCTGTGAAAGGGAT
TGAGTTATCCATTCCTTTCCAAAGAATGACGTATCAAGAAGCAAAAGAGTTTTACGGGACAGATAAACCAGATTTACGCT
TTGGTTTGCGTCTTAAAAATTGTTGCGAATACGCCAAAAAATTCTCCTTTTCCATTTTCTTAGATCAGTTGGCACAAGGA
GGAACAGTTAAAGGATTTTGTGTCCCTGGTGGCGCTGATATCTCAAGAAAACAGTTGGATGTCTATACTGATTTTGTGAA
GCGTTATGGAGCCATGGGATTAGTGTGGATTAAAAACCAGGATGGGGGGATTTCTTCGAATGTAGCGAAATTTGCTTCAG
AAGAGGTTTTCCATGAAATGTTCGAAGCTTTTGAGGCCAAAGATCAAGATATTTTATTATTGATAGCAGCTCCAGAGGCT
ATTGCGAACCAGTCATTGGATCATTTACGCAGATTAATCGCAAAAGAGCGGCAGCTTTATGATGCAGAACAATACAATTT
TGTGTGGATTACAGACTTCCCTCTTTTTGCTAAGGAAGAAGGCGAGCTGTGCTCAGAGCATCATCCATTCACAGCTCCAT
TAGAAGAGGATATTCCTCTGTTGGATAAGGATCCTCTGTCCGTTCGCTCATCAAGCTATGATTTGGTGCTGAATGGATAT
GAAATAGCTTCCGGTTCTCAGCGTATACATAATCCGGATTTGCAAAATAAAATATTTTCTTTATTAAGGCTGTCGCAAGA
AAGTGTGAAAGAAAAGTTCGGATTTTTTATCGATGCATTAAGTTTTGGGACGCCGCCTCATTTAGGGATTGCTTTAGGAT
TGGACCGCATTATGATGGTTCTTACAGGGGCAGAAACTATTCGAGAGGTTATTGCGTTTCCTAAGACGCAGAAAGCTGGA
GATTTAATGATGTCGGCACCTTCAGAAATTTTGCCGATACAATTAAAAGAATTGGGGCTGAAACTGTAA

Upstream 100 bases:

>100_bases
GGCGAGCAAGAGCTGTCTACGAAACAGTTTATAGTTAAAGATATGTCTCTACATCAAAGCTTTTCAGGGGCTCAACAAGA
TGTAGAACAAAGGTTGGTTT

Downstream 100 bases:

>100_bases
TATCGTTTCTGCTCTAAAAGTTGAGAAAACGAGCTTTTTTTTACAGTGGGTGATAGGTGCAGGTCTTTTACTTGAGTAAA
TGGTTTTAAAAAAAGCGCGT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKYRTHKCNELSLNHVGEHVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCRQEENPELHRLMDQVRSEWVLCVEGLVCA
RLGGMENPNLVTGSIEVEVSHLEVLSRAQNLPFSISDEHINVNEELRLTYRYLDMRRGDIVDRLMCRHKVMLACRQYLDE
QGFTEVVTPVLGKSTPEGARDYLVPSRIYPGNFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFSQIDM
EMSFGGPEDLFPIVEELVTRLFAVKGIELSIPFQRMTYQEAKEFYGTDKPDLRFGLRLKNCCEYAKKFSFSIFLDQLAQG
GTVKGFCVPGGADISRKQLDVYTDFVKRYGAMGLVWIKNQDGGISSNVAKFASEEVFHEMFEAFEAKDQDILLLIAAPEA
IANQSLDHLRRLIAKERQLYDAEQYNFVWITDFPLFAKEEGELCSEHHPFTAPLEEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNPDLQNKIFSLLRLSQESVKEKFGFFIDALSFGTPPHLGIALGLDRIMMVLTGAETIREVIAFPKTQKAG
DLMMSAPSEILPIQLKELGLKL

Sequences:

>Translated_582_residues
MKYRTHKCNELSLNHVGEHVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCRQEENPELHRLMDQVRSEWVLCVEGLVCA
RLGGMENPNLVTGSIEVEVSHLEVLSRAQNLPFSISDEHINVNEELRLTYRYLDMRRGDIVDRLMCRHKVMLACRQYLDE
QGFTEVVTPVLGKSTPEGARDYLVPSRIYPGNFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFSQIDM
EMSFGGPEDLFPIVEELVTRLFAVKGIELSIPFQRMTYQEAKEFYGTDKPDLRFGLRLKNCCEYAKKFSFSIFLDQLAQG
GTVKGFCVPGGADISRKQLDVYTDFVKRYGAMGLVWIKNQDGGISSNVAKFASEEVFHEMFEAFEAKDQDILLLIAAPEA
IANQSLDHLRRLIAKERQLYDAEQYNFVWITDFPLFAKEEGELCSEHHPFTAPLEEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNPDLQNKIFSLLRLSQESVKEKFGFFIDALSFGTPPHLGIALGLDRIMMVLTGAETIREVIAFPKTQKAG
DLMMSAPSEILPIQLKELGLKL
>Mature_582_residues
MKYRTHKCNELSLNHVGEHVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCRQEENPELHRLMDQVRSEWVLCVEGLVCA
RLGGMENPNLVTGSIEVEVSHLEVLSRAQNLPFSISDEHINVNEELRLTYRYLDMRRGDIVDRLMCRHKVMLACRQYLDE
QGFTEVVTPVLGKSTPEGARDYLVPSRIYPGNFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFSQIDM
EMSFGGPEDLFPIVEELVTRLFAVKGIELSIPFQRMTYQEAKEFYGTDKPDLRFGLRLKNCCEYAKKFSFSIFLDQLAQG
GTVKGFCVPGGADISRKQLDVYTDFVKRYGAMGLVWIKNQDGGISSNVAKFASEEVFHEMFEAFEAKDQDILLLIAAPEA
IANQSLDHLRRLIAKERQLYDAEQYNFVWITDFPLFAKEEGELCSEHHPFTAPLEEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNPDLQNKIFSLLRLSQESVKEKFGFFIDALSFGTPPHLGIALGLDRIMMVLTGAETIREVIAFPKTQKAG
DLMMSAPSEILPIQLKELGLKL

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=592, Percent_Identity=41.3851351351351, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI5031815, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194272210, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI45439306, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=45.6558773424191, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=27.5862068965517, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=290, Percent_Identity=26.2068965517241, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=35.1170568561873, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17535925, Length=306, Percent_Identity=24.8366013071895, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=306, Percent_Identity=24.8366013071895, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=306, Percent_Identity=24.8366013071895, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=286, Percent_Identity=25.1748251748252, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=358, Percent_Identity=26.8156424581006, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=617, Percent_Identity=32.2528363047002, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321807, Length=256, Percent_Identity=27.34375, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=247, Percent_Identity=26.7206477732794, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=25, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=37.5, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=27.8008298755187, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640849, Length=275, Percent_Identity=26.5454545454545, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640851, Length=275, Percent_Identity=26.5454545454545, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CHLMU (Q9PJK0)

Other databases:

- EMBL:   AE002160
- PIR:   E81660
- RefSeq:   NP_297202.1
- ProteinModelPortal:   Q9PJK0
- SMR:   Q9PJK0
- GeneID:   1246197
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0829
- TIGR:   TC_0829
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q9PJK0
- ProtClustDB:   PRK00476
- BioCyc:   CMUR243161:TC_0829-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66387; Mature: 66387

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRTHKCNELSLNHVGEHVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCRQEENPELH
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCHHH
RLMDQVRSEWVLCVEGLVCARLGGMENPNLVTGSIEVEVSHLEVLSRAQNLPFSISDEHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCC
NVNEELRLTYRYLDMRRGDIVDRLMCRHKVMLACRQYLDEQGFTEVVTPVLGKSTPEGAR
CCCHHHEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
DYLVPSRIYPGNFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFSQIDM
HCCCCCEECCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHEEE
EMSFGGPEDLFPIVEELVTRLFAVKGIELSIPFQRMTYQEAKEFYGTDKPDLRFGLRLKN
EECCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCCEECCHHHH
CCEYAKKFSFSIFLDQLAQGGTVKGFCVPGGADISRKQLDVYTDFVKRYGAMGLVWIKNQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECC
DGGISSNVAKFASEEVFHEMFEAFEAKDQDILLLIAAPEAIANQSLDHLRRLIAKERQLY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHC
DAEQYNFVWITDFPLFAKEEGELCSEHHPFTAPLEEDIPLLDKDPLSVRSSSYDLVLNGY
CCCCCCEEEEECCCCEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECE
EIASGSQRIHNPDLQNKIFSLLRLSQESVKEKFGFFIDALSFGTPPHLGIALGLDRIMMV
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTGAETIREVIAFPKTQKAGDLMMSAPSEILPIQLKELGLKL
HHCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MKYRTHKCNELSLNHVGEHVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCRQEENPELH
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCHHH
RLMDQVRSEWVLCVEGLVCARLGGMENPNLVTGSIEVEVSHLEVLSRAQNLPFSISDEHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCC
NVNEELRLTYRYLDMRRGDIVDRLMCRHKVMLACRQYLDEQGFTEVVTPVLGKSTPEGAR
CCCHHHEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
DYLVPSRIYPGNFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFSQIDM
HCCCCCEECCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHEEE
EMSFGGPEDLFPIVEELVTRLFAVKGIELSIPFQRMTYQEAKEFYGTDKPDLRFGLRLKN
EECCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCCEECCHHHH
CCEYAKKFSFSIFLDQLAQGGTVKGFCVPGGADISRKQLDVYTDFVKRYGAMGLVWIKNQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECC
DGGISSNVAKFASEEVFHEMFEAFEAKDQDILLLIAAPEAIANQSLDHLRRLIAKERQLY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHC
DAEQYNFVWITDFPLFAKEEGELCSEHHPFTAPLEEDIPLLDKDPLSVRSSSYDLVLNGY
CCCCCCEEEEECCCCEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECE
EIASGSQRIHNPDLQNKIFSLLRLSQESVKEKFGFFIDALSFGTPPHLGIALGLDRIMMV
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTGAETIREVIAFPKTQKAGDLMMSAPSEILPIQLKELGLKL
HHCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935