Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 15835361

GI number: 15835361

Start: 890644

End: 891300

Strand: Direct

Name: ispD

Synonym: TC0747

Alternate gene names: 15835361

Gene position: 890644-891300 (Clockwise)

Preceding gene: 15835360

Following gene: 15835364

Centisome position: 83.01

GC content: 44.44

Gene sequence:

>657_bases
ATGAACCTTAGCTGTTCCCTCGTATTGTTAGGAGGAGGGAGAGGCGAGCGTTTTAACTCCCCCCAACCCAAGCAATACAC
TCCTCTTTGTGGGGAGCCGCTAATTCTTCATGCCCTACATTCCTATCAAAGCCTCCCCTTCATTCAAGAAATCGTTGTTG
TCTGCGAAGAACACTACCAAGAATTGTTCTCCCCCTACTCCGTGAAATTTGCTTCACCAGGAGCTCTACGTCAAGATTCC
GTCTTTTCTGGATTACAACAAGTTTCTTTGCCATGGGTATGCGTACATGATGGCGTTCGCCCTTTCGTCTATGCAAATGA
AGTTTCTGAAGTCTGCTCCGCAGCTCTTAAAACTGGAGCAGCAGCTCTCGCCACCTCCGCAACTTATACAATAAAATCTC
GTACTCCAGTACGCACCTTAGATAGAGATGCTGTGGCAGTAATTCATACTCCCCAATGCATTAATACAGAAATACTGAAA
GAAGGTCTTCTTCTTGCTAACATGATGGATTTTACCTTATCTGATGATTCGGAAGCCGCTGAACTACTTGGTATAGAACC
TACTCTGGTATTCAGTAACAGAGTGCAAATGAAGATTACCTACCCTGAAGATCTTTTATTTGCCGAAGCTCTTCTCTCTA
AGGCTCACATTCGTTAA

Upstream 100 bases:

>100_bases
CCTCAAGAGAAAAGGCTCTTGTATGTAAACCGAGGACTAGGAGGATGGAAACGCATACGGTTTTTCTCTCCCCCGGAAAT
TTGTATAATGAGGTGTCTCT

Downstream 100 bases:

>100_bases
TCAAGCTTCTCATGACAAAAAAAATTATTTTACGAATTGCCTATCAAGGGACCGCTTACTCTGGATGGCAATATCAACCT
AATGCTCTCTCGATTCAAGA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNLSCSLVLLGGGRGERFNSPQPKQYTPLCGEPLILHALHSYQSLPFIQEIVVVCEEHYQELFSPYSVKFASPGALRQDS
VFSGLQQVSLPWVCVHDGVRPFVYANEVSEVCSAALKTGAAALATSATYTIKSRTPVRTLDRDAVAVIHTPQCINTEILK
EGLLLANMMDFTLSDDSEAAELLGIEPTLVFSNRVQMKITYPEDLLFAEALLSKAHIR

Sequences:

>Translated_218_residues
MNLSCSLVLLGGGRGERFNSPQPKQYTPLCGEPLILHALHSYQSLPFIQEIVVVCEEHYQELFSPYSVKFASPGALRQDS
VFSGLQQVSLPWVCVHDGVRPFVYANEVSEVCSAALKTGAAALATSATYTIKSRTPVRTLDRDAVAVIHTPQCINTEILK
EGLLLANMMDFTLSDDSEAAELLGIEPTLVFSNRVQMKITYPEDLLFAEALLSKAHIR
>Mature_218_residues
MNLSCSLVLLGGGRGERFNSPQPKQYTPLCGEPLILHALHSYQSLPFIQEIVVVCEEHYQELFSPYSVKFASPGALRQDS
VFSGLQQVSLPWVCVHDGVRPFVYANEVSEVCSAALKTGAAALATSATYTIKSRTPVRTLDRDAVAVIHTPQCINTEILK
EGLLLANMMDFTLSDDSEAAELLGIEPTLVFSNRVQMKITYPEDLLFAEALLSKAHIR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1789104, Length=234, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CHLMU (Q9PJT1)

Other databases:

- EMBL:   AE002160
- PIR:   C81669
- RefSeq:   NP_297120.1
- ProteinModelPortal:   Q9PJT1
- GeneID:   1246110
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0747
- TIGR:   TC_0747
- HOGENOM:   HBG672839
- OMA:   NTIPREN
- PhylomeDB:   Q9PJT1
- ProtClustDB:   PRK00155
- BioCyc:   CMUR243161:TC_0747-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23957; Mature: 23957

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSCSLVLLGGGRGERFNSPQPKQYTPLCGEPLILHALHSYQSLPFIQEIVVVCEEHYQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ELFSPYSVKFASPGALRQDSVFSGLQQVSLPWVCVHDGVRPFVYANEVSEVCSAALKTGA
HHCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEHHHHHHHHHHHHHCCH
AALATSATYTIKSRTPVRTLDRDAVAVIHTPQCINTEILKEGLLLANMMDFTLSDDSEAA
HHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEECCCCHHH
ELLGIEPTLVFSNRVQMKITYPEDLLFAEALLSKAHIR
HHHCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLSCSLVLLGGGRGERFNSPQPKQYTPLCGEPLILHALHSYQSLPFIQEIVVVCEEHYQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ELFSPYSVKFASPGALRQDSVFSGLQQVSLPWVCVHDGVRPFVYANEVSEVCSAALKTGA
HHCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEHHHHHHHHHHHHHCCH
AALATSATYTIKSRTPVRTLDRDAVAVIHTPQCINTEILKEGLLLANMMDFTLSDDSEAA
HHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEECCCCHHH
ELLGIEPTLVFSNRVQMKITYPEDLLFAEALLSKAHIR
HHHCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935