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The map label for this gene is aroA

Identifier: 15835260

GI number: 15835260

Start: 771952

End: 773277

Strand: Direct

Name: aroA

Synonym: TC0645

Alternate gene names: 15835260

Gene position: 771952-773277 (Clockwise)

Preceding gene: 15835252

Following gene: 15835261

Centisome position: 71.95

GC content: 44.34

Gene sequence:

>1326_bases
GTGGTCCCTTTGAACCAAGCCTTTCTGATTTCCCCTTCGGCTCCTTATGGAGAATTTTCCATCCCTCCTTCAAAATCTCA
CTCTCTACGAGCCATCCTTTTTGCCTCGCTATCTAAAGGGACCTCCATCATAAACAATAGTCTTTCCTCTCCCGACACCG
ACACTATGCTCTCAGCATGCAAAAAATTTGGAGCTCGTATTACGAGAGTTGGAGAAACTCTGCATATACAAGGGAATCCT
CCTCCTTATTCCCAATACTCCTCTCACCATTTCCATATGGGAAACTCTGGCATAGCTCTACGATTCCTAACGGCTCTTTC
TTCTTTATCCCCATCCCCTATCCTCATTACAGGAGCCCACACACTCAAAAGACGCCCTATCGAACCTCTTCTTTCTAGCT
TAGAACAACTTGGATCGGAAATTCGTCAAAAGAAATCTTCTTCCATTCCTTTTGTTATTCGAGGCCCTATATCCTCAGGA
CATGTTACTATCTCTGGACAAGATTCCCAATATGCATCTGCCTTAGCCATTACCGCAGCAGTAGCGCCCCATCCTCTTTC
CTTTTCCATTGAAAATCTGAAAGAACGTCCCTGGTTTGACCTAACCCTAGACTGGCTCCATTCTTTAAACATCTCCTTTT
CCAGAGAACAAGATTCTTTATTCTTCCCCGGAGCACAATCCATAAAAAGCTTTTCCTACTCTGTTCCAGGAGATTACAGT
TCCGCTGCTTTTCTAGCTGCTCTGGGGTTGCTATCCTCATCCTCAAACCCAACAGTGCTATATAATCTTCCCTCCAAAGA
CCCTCAGGGAGACAAACAACTCTTCTCTCTTTTAAAAAGCTTAGGAGCAGACATTGTTATTGAAAAAGACCACATTGAAA
TCCGCCCATCTTCCTTCTCTGGAGGAGTCATTGATATGGATCCTTTTATAGATGCTCTTCCTATACTAGCTGTTCTCTGC
TGCTTTGCCAAAAACCCATCGCATTTGTATAATGCGCTAGGAGCAAGAGACAAAGAAAGCAATCGCATTGAAGCTATTGC
CCATGAGTTGCGGAAGATGGGTGGCTCAGTTCATCCTACTCAAGATGGTCTGTATATAGAGCCTTCTCGATTGCATGGAG
CAGTTGTTCATTCTCACAATGATCATCGCATTGCCATGGCTCTTGCTGTAGCAGGAGTTCATGCCTCTTCCGGACAAACC
CTCCTTTGTAACACACAATGTGTAAATAAGAGTTTTCCTCATTTCGTGATTGCTGCACAGACACTACATGCCAACATTCG
ACACCACCAAGCAGATTTTTCTTTGCGGTCTTCCCTCTGTAGGTAA

Upstream 100 bases:

>100_bases
AAAGTAGCACATCTCATTTTTATCATATCAAACAAGTATATCATGAAAAAAAGTCTTCTAAAGATTACATTAGCTCCTCA
TCTTTAATCTTTTTCAACGT

Downstream 100 bases:

>100_bases
AACCTCTTTTGGGAGACTTTTAGCTCAGTTTTTATCGCTTCCCTTTTTTGACACGGATCATCTTCTTTCTGCTCGTTTTC
ATGGGGATTCCCCTAAAGCC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MVPLNQAFLISPSAPYGEFSIPPSKSHSLRAILFASLSKGTSIINNSLSSPDTDTMLSACKKFGARITRVGETLHIQGNP
PPYSQYSSHHFHMGNSGIALRFLTALSSLSPSPILITGAHTLKRRPIEPLLSSLEQLGSEIRQKKSSSIPFVIRGPISSG
HVTISGQDSQYASALAITAAVAPHPLSFSIENLKERPWFDLTLDWLHSLNISFSREQDSLFFPGAQSIKSFSYSVPGDYS
SAAFLAALGLLSSSSNPTVLYNLPSKDPQGDKQLFSLLKSLGADIVIEKDHIEIRPSSFSGGVIDMDPFIDALPILAVLC
CFAKNPSHLYNALGARDKESNRIEAIAHELRKMGGSVHPTQDGLYIEPSRLHGAVVHSHNDHRIAMALAVAGVHASSGQT
LLCNTQCVNKSFPHFVIAAQTLHANIRHHQADFSLRSSLCR

Sequences:

>Translated_441_residues
MVPLNQAFLISPSAPYGEFSIPPSKSHSLRAILFASLSKGTSIINNSLSSPDTDTMLSACKKFGARITRVGETLHIQGNP
PPYSQYSSHHFHMGNSGIALRFLTALSSLSPSPILITGAHTLKRRPIEPLLSSLEQLGSEIRQKKSSSIPFVIRGPISSG
HVTISGQDSQYASALAITAAVAPHPLSFSIENLKERPWFDLTLDWLHSLNISFSREQDSLFFPGAQSIKSFSYSVPGDYS
SAAFLAALGLLSSSSNPTVLYNLPSKDPQGDKQLFSLLKSLGADIVIEKDHIEIRPSSFSGGVIDMDPFIDALPILAVLC
CFAKNPSHLYNALGARDKESNRIEAIAHELRKMGGSVHPTQDGLYIEPSRLHGAVVHSHNDHRIAMALAVAGVHASSGQT
LLCNTQCVNKSFPHFVIAAQTLHANIRHHQADFSLRSSLCR
>Mature_441_residues
MVPLNQAFLISPSAPYGEFSIPPSKSHSLRAILFASLSKGTSIINNSLSSPDTDTMLSACKKFGARITRVGETLHIQGNP
PPYSQYSSHHFHMGNSGIALRFLTALSSLSPSPILITGAHTLKRRPIEPLLSSLEQLGSEIRQKKSSSIPFVIRGPISSG
HVTISGQDSQYASALAITAAVAPHPLSFSIENLKERPWFDLTLDWLHSLNISFSREQDSLFFPGAQSIKSFSYSVPGDYS
SAAFLAALGLLSSSSNPTVLYNLPSKDPQGDKQLFSLLKSLGADIVIEKDHIEIRPSSFSGGVIDMDPFIDALPILAVLC
CFAKNPSHLYNALGARDKESNRIEAIAHELRKMGGSVHPTQDGLYIEPSRLHGAVVHSHNDHRIAMALAVAGVHASSGQT
LLCNTQCVNKSFPHFVIAAQTLHANIRHHQADFSLRSSLCR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=408, Percent_Identity=31.3725490196078, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320332, Length=442, Percent_Identity=26.9230769230769, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_CHLMU (Q9PK28)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_297019.1
- ProteinModelPortal:   Q9PK28
- GeneID:   1246006
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0645
- TIGR:   TC_0645
- HOGENOM:   HBG646626
- OMA:   SSQFITS
- PhylomeDB:   Q9PK28
- ProtClustDB:   PRK02427
- BioCyc:   CMUR243161:TC_0645-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47728; Mature: 47728

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPLNQAFLISPSAPYGEFSIPPSKSHSLRAILFASLSKGTSIINNSLSSPDTDTMLSAC
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEHEEHHHHCCHHHHHCCCCCCCHHHHHHHH
KKFGARITRVGETLHIQGNPPPYSQYSSHHFHMGNSGIALRFLTALSSLSPSPILITGAH
HHHHHHEEECCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEECCH
TLKRRPIEPLLSSLEQLGSEIRQKKSSSIPFVIRGPISSGHVTISGQDSQYASALAITAA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHH
VAPHPLSFSIENLKERPWFDLTLDWLHSLNISFSREQDSLFFPGAQSIKSFSYSVPGDYS
HCCCCCEEEHHHHCCCCCEEEEHHHHHHCCCEEECCCCCEECCCHHHHHHCCCCCCCCCC
SAAFLAALGLLSSSSNPTVLYNLPSKDPQGDKQLFSLLKSLGADIVIEKDHIEIRPSSFS
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEECCCCCC
GGVIDMDPFIDALPILAVLCCFAKNPSHLYNALGARDKESNRIEAIAHELRKMGGSVHPT
CCEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
QDGLYIEPSRLHGAVVHSHNDHRIAMALAVAGVHASSGQTLLCNTQCVNKSFPHFVIAAQ
CCCEEECHHHHCEEEEECCCCCEEEEEEEEHHCCCCCCCEEEEECHHHCCCCCCEEEEHH
TLHANIRHHQADFSLRSSLCR
HHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MVPLNQAFLISPSAPYGEFSIPPSKSHSLRAILFASLSKGTSIINNSLSSPDTDTMLSAC
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEHEEHHHHCCHHHHHCCCCCCCHHHHHHHH
KKFGARITRVGETLHIQGNPPPYSQYSSHHFHMGNSGIALRFLTALSSLSPSPILITGAH
HHHHHHEEECCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEECCH
TLKRRPIEPLLSSLEQLGSEIRQKKSSSIPFVIRGPISSGHVTISGQDSQYASALAITAA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHH
VAPHPLSFSIENLKERPWFDLTLDWLHSLNISFSREQDSLFFPGAQSIKSFSYSVPGDYS
HCCCCCEEEHHHHCCCCCEEEEHHHHHHCCCEEECCCCCEECCCHHHHHHCCCCCCCCCC
SAAFLAALGLLSSSSNPTVLYNLPSKDPQGDKQLFSLLKSLGADIVIEKDHIEIRPSSFS
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEECCCCCC
GGVIDMDPFIDALPILAVLCCFAKNPSHLYNALGARDKESNRIEAIAHELRKMGGSVHPT
CCEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
QDGLYIEPSRLHGAVVHSHNDHRIAMALAVAGVHASSGQTLLCNTQCVNKSFPHFVIAAQ
CCCEEECHHHHCEEEEECCCCCEEEEEEEEHHCCCCCCCEEEEECHHHCCCCCCEEEEHH
TLHANIRHHQADFSLRSSLCR
HHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935