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The map label for this gene is ptsI

Identifier: 15835230

GI number: 15835230

Start: 737089

End: 738792

Strand: Reverse

Name: ptsI

Synonym: TC0613

Alternate gene names: 15835230

Gene position: 738792-737089 (Counterclockwise)

Preceding gene: 15835231

Following gene: 15835220

Centisome position: 68.86

GC content: 39.91

Gene sequence:

>1704_bases
ATGGGCGCTACGAGTCAAAAGGAATTGCAGCAAGAGTTTGTGATTGTAGGAGAGCCTGTTGTTCCTGGAATAGGATTGGG
TAAATCTTTATTGCTAGGAAAATCTTCTTTACGAATACGAGAGTTGACTCTCCCTCAGGAAGAAGTGGAGCATGAGATCA
ATCGTTACTATAAGGCTTTAAAAGAATCTCGATCAGATCTTGCTGCTTTGGAAAAAAAAGCTAAGGGGAAGCAAGGGTAT
CAGGAAATTGCTTCTATTTTACAGGCACATCTGGAAATTATAAAAGATCCTCTTCTTACGGAAGAAGTTGTTAAAACCAT
TAGAAAAGATCGTAAGAATGCCGAGTTTGTTTTTTCTTCTGTAATGGGGGAGATAGAGAAATCTTTATGTGCTGTGCAAA
AAACAACAGCTATAGCTGTAGATCGTATTCAGGATATTCATGATATTTCTAATCGTGTGATTGGACATTTATGTTGTCAG
CATAAAAGTTCCTTGGGAGAGTCCGATCAGAACTTGATCGTTTTTTCAGAAGAGCTCACTCCTTCGGAAGCTGCCAATGC
AAATCCCAAGTACATAAGGGGATTTGTATCTTTAGAGGGTTCAAAAACTTCGCATACAGCAATTGTGTCATTAGCGAAAA
ATATTCCTTATGTGGCTAATTTTTCTGCCGAGTCTTGGCAAAGAATCAGAGAATATAACGGAAACTTAGTTCTGATTAAT
GGGGAGAAAGGAGAGATAACGTTTAATCCTAAGTTGAGTACGATCCAAGCCTATTATCGTAAGCAATCAGCAGTGTCAAT
GACTGTCCCTATTCAGATAGAAAAAACACAGCCTCTCATTTCTCTTTCTGCCCAAATAGTAGGAGTGGATGAATTAGGGT
CTATTTCAAGAGAGTTTCCAGGAACTACTATCGGTTTATTCCGTTCGGAATTCATGGCATTTTCTTTAGGAAGGCTTCCT
TTTGTTGAGGAACAAGTTGCTGAATATACCAAATTAGTTCAGTTTTCCTGTTCTGATATCAATGTGCTGCGATTATTTGA
TTTTGGAGAGGATAAGGTGTGTCCTTTTATAGCCTCTGCTCATCGGTCTGTACGTTGGCTATTGGAGCAAGAGACTATTT
TAAGAGGACAATTGCAAGCGATCGCTATAGCTTCTCGACAAGGGAAATTAAAAGTTCTGATTCCAGGAGTTCTGGATGCT
TCTGAGATTATTTTAGTGAAGCAGATGTTTCAAGAGGAAGTGCAGTTACAGAATGGGATTAGTGAGAATATCATTTGGGG
AAGTATGATAGAGATCCCCTCTGCAGTTTGGATGATAGAGGAGATCCTACAGGAAAGTTCTTTTATTGCTTTAGGAACAA
ATGATCTTGCTCAGTATACACTGGGAATGTCTAGAGAACGCTCTCTCCCAGGTAATTGGAAACAAGTGCCCCACCCTTCT
GTCATTAGAATGATTCATTATGTTGCCGCGCGAGCAAAACAAAGAAATATTCCAGTATCCGTATGTGGGGAGATGGCTGG
AGATCATCTCCTTTTGCCCATGTTCATAGGGTTTGGAGTGCGGGAATTATCTGTTGTGGCTCCCGCAATACATTCTTTAA
AAATGAGATTGTTAGCCTTGAATTCAAAGGAGTGCTCTCGACTAGCAAAACAGCTATTGCGGGCAAGAACATATGAAGAG
GTTCACAAACTCCTGAACATGTAA

Upstream 100 bases:

>100_bases
TTATAATGGAGAGATCACTGTGCGAATAAAAGGCCCTTCGGCTTCTCGTGTAATGCAAAAACTAGCAGAGGTTTTTAATT
CTGGATTCGGAGAGTTATAA

Downstream 100 bases:

>100_bases
TAGGAGGATTAGAAAGGCATTCCCATGTGCATAGCGGACATTTCGCTATCCAAAGCAGCTTTTGCTGCTTTAAATGCTGC
ACGAAATAGGTCTGCTACAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MGATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKALKESRSDLAALEKKAKGKQGY
QEIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSSVMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQ
HKSSLGESDQNLIVFSEELTPSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLIN
GEKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFPGTTIGLFRSEFMAFSLGRLP
FVEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASAHRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDA
SEIILVKQMFQEEVQLQNGISENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPS
VIRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLALNSKECSRLAKQLLRARTYEE
VHKLLNM

Sequences:

>Translated_567_residues
MGATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKALKESRSDLAALEKKAKGKQGY
QEIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSSVMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQ
HKSSLGESDQNLIVFSEELTPSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLIN
GEKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFPGTTIGLFRSEFMAFSLGRLP
FVEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASAHRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDA
SEIILVKQMFQEEVQLQNGISENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPS
VIRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLALNSKECSRLAKQLLRARTYEE
VHKLLNM
>Mature_566_residues
GATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKALKESRSDLAALEKKAKGKQGYQ
EIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSSVMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQH
KSSLGESDQNLIVFSEELTPSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLING
EKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFPGTTIGLFRSEFMAFSLGRLPF
VEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASAHRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDAS
EIILVKQMFQEEVQLQNGISENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPSV
IRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLALNSKECSRLAKQLLRARTYEEV
HKLLNM

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=26.8551236749117, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1788726, Length=584, Percent_Identity=26.3698630136986, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=27.0750988142292, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1789193, Length=539, Percent_Identity=26.3450834879406, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI226510935, Length=162, Percent_Identity=26.5432098765432, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_CHLMU (Q9PK57)

Other databases:

- EMBL:   AE002160
- PIR:   F81682
- RefSeq:   NP_296989.1
- ProteinModelPortal:   Q9PK57
- SMR:   Q9PK57
- GeneID:   1245975
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0613
- TIGR:   TC_0613
- HOGENOM:   HBG414040
- OMA:   LTEPTIL
- ProtClustDB:   CLSK871320
- BioCyc:   CMUR243161:TC_0613-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 63312; Mature: 63181

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 205-205 ACT_SITE 502-502 BINDING 308-308 BINDING 344-344 BINDING 431-431 BINDING 452-452 BINDING 453-453 BINDING 454-454 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKAL
CCCCCHHHHHHHHEEECCCCCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
KESRSDLAALEKKAKGKQGYQEIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
VMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQHKSSLGESDQNLIVFSEELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLIN
CCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEE
GEKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFP
CCCCEEEECCCHHHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHHEEEHHHHHHHHHHCC
GTTIGLFRSEFMAFSLGRLPFVEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
HRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDASEIILVKQMFQEEVQLQNGI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPS
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCHH
VIRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLAL
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
NSKECSRLAKQLLRARTYEEVHKLLNM
CCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKAL
CCCCHHHHHHHHEEECCCCCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
KESRSDLAALEKKAKGKQGYQEIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
VMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQHKSSLGESDQNLIVFSEELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLIN
CCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEE
GEKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFP
CCCCEEEECCCHHHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHHEEEHHHHHHHHHHCC
GTTIGLFRSEFMAFSLGRLPFVEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
HRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDASEIILVKQMFQEEVQLQNGI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPS
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCHH
VIRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLAL
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
NSKECSRLAKQLLRARTYEEVHKLLNM
CCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935