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The map label for this gene is gcvH

Identifier: 15835172

GI number: 15835172

Start: 663051

End: 663404

Strand: Reverse

Name: gcvH

Synonym: TC0554

Alternate gene names: 15835172

Gene position: 663404-663051 (Counterclockwise)

Preceding gene: 15835173

Following gene: 15835167

Centisome position: 61.83

GC content: 38.7

Gene sequence:

>354_bases
ATGAAAGGTAAAAAATATTATTCCGACTACCATGTATGGATAGAGCCTATTCATTCTCAAATTGTGAGACTTGGTTTATC
ATCTCGGATGCAGGAGCATTTGGGGAATATTTTGCATATAGATCTGCCTTCTTTAGGAGCTTCTATTAAAGAAGGAGAAG
AACTTTGTGTTTTAGAGTCTTCGAAATCTGCTATTGAAGTGCTTTCTCCTGTTTCTGGAGAAGTGATAGAAGTTAATATT
GCGCTTGAAGACGATACTCATCCAATTAATCATTCTGCAGAATCCGAGGGCTGGTTCGTTGTTCTGCAATTGTCAGAAGA
TTTCGATGGAGAGCGCTTTTCCCTAGATCCTTAG

Upstream 100 bases:

>100_bases
TTGCAAAAAATCATTTGAATGAAGAGCTTTTAAGACCTTATTTATCAGAGATTTTTTTCCAAAACGAGAGTTAGAAATAT
TGAAATTGAATGGGACATGT

Downstream 100 bases:

>100_bases
GGTTTGTTTACGCTTTGCCCTGTAGTTTAAGCTTTGTGGTTTGAGCTGAGGCGTTCTTGCGCGGAGGTTGTTTTTGATTT
ATTGGTGGAAGTGTCTGAAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKGKKYYSDYHVWIEPIHSQIVRLGLSSRMQEHLGNILHIDLPSLGASIKEGEELCVLESSKSAIEVLSPVSGEVIEVNI
ALEDDTHPINHSAESEGWFVVLQLSEDFDGERFSLDP

Sequences:

>Translated_117_residues
MKGKKYYSDYHVWIEPIHSQIVRLGLSSRMQEHLGNILHIDLPSLGASIKEGEELCVLESSKSAIEVLSPVSGEVIEVNI
ALEDDTHPINHSAESEGWFVVLQLSEDFDGERFSLDP
>Mature_117_residues
MKGKKYYSDYHVWIEPIHSQIVRLGLSSRMQEHLGNILHIDLPSLGASIKEGEELCVLESSKSAIEVLSPVSGEVIEVNI
ALEDDTHPINHSAESEGWFVVLQLSEDFDGERFSLDP

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=101, Percent_Identity=34.6534653465347, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI89057342, Length=101, Percent_Identity=34.6534653465347, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789271, Length=101, Percent_Identity=33.6633663366337, Blast_Score=73, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17551294, Length=103, Percent_Identity=32.0388349514563, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507493, Length=100, Percent_Identity=30, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319272, Length=106, Percent_Identity=32.0754716981132, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17865652, Length=104, Percent_Identity=37.5, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CHLMU (Q9PKB2)

Other databases:

- EMBL:   AE002160
- PIR:   F81690
- RefSeq:   NP_296931.1
- ProteinModelPortal:   Q9PKB2
- SMR:   Q9PKB2
- GeneID:   1245914
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0554
- TIGR:   TC_0554
- HOGENOM:   HBG693789
- OMA:   FCKEGEV
- ProtClustDB:   PRK00624
- BioCyc:   CMUR243161:TC_0554-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017514
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR03077

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13088; Mature: 13088

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKKYYSDYHVWIEPIHSQIVRLGLSSRMQEHLGNILHIDLPSLGASIKEGEELCVLES
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEC
SKSAIEVLSPVSGEVIEVNIALEDDTHPINHSAESEGWFVVLQLSEDFDGERFSLDP
CCCCEEHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCC
>Mature Secondary Structure
MKGKKYYSDYHVWIEPIHSQIVRLGLSSRMQEHLGNILHIDLPSLGASIKEGEELCVLES
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEC
SKSAIEVLSPVSGEVIEVNIALEDDTHPINHSAESEGWFVVLQLSEDFDGERFSLDP
CCCCEEHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935