Click here to switch to the map view.

The map label for this gene is dnaA

Identifier: 15835165

GI number: 15835165

Start: 656551

End: 657918

Strand: Direct

Name: dnaA

Synonym: TC0547

Alternate gene names: 15835165

Gene position: 656551-657918 (Clockwise)

Preceding gene: 15835164

Following gene: 15835166

Centisome position: 61.19

GC content: 37.94

Gene sequence:

>1368_bases
ATGTTAACTTGCAACGATTGCAGTACGTGGGAACAGTTCGTTAATTATATCAAGACCCGCTGCTCCAAAACAGCTTTTGA
AAACTGGATTGCTCCTATCCAAGTATTAGAAGAATCTCGAGAAAAAATTCGGTTAGAAATTCCGAATATATTTGTCCAAA
GTTATCTGTTAGATAACTACAAAAAGGATCTTTGTTCTTTTGTGCCTCTTGATGCAGAAGGCAATCCTGCGTTGGAGTTT
GTTGTTTCGGAGATTAAGCGTTCTTCTCCCCAAATAGCCGCGTCTGTTACTAAGCCTGCCGTCGAAGTTTCAGAAGAAAA
TAAAGATTTTCAACTCAAACTTAATGGAGCTTATCGCTTTGATAATTTCATTGAAGGTCCCTCCAATCAGTTTGTAAAAT
CTGCTGCCTTAGGGATTGCTGCTCGCCCAGGAAGATCTTACAACCCCTTATTTATTCATGGCGGAGTGGGATTAGGTAAG
ACTCACCTACTTCATGCTGTTGGACATTATGTTCGCGAACACCATAAGAATCTCCGCATACACTGCATTACAACAGAAGC
TTTTATCAATGATTTAGTTCATCATTTGCGAGTTAAATCTATTGATAAGATGAAAAATTTTTATAGATCTTTAGATCTTC
TCCTTGTCGATGATATTCAGTTCTTACAAAACCGACAAAATTTTGAAGAAGAGTTCTGTAACACTTTTGAAACTCTAATC
CACCTGTCCAAACAGATTGTTGTAACTAGTGATAAACCCCCAGGCCAACTCAAATTGTCCGAACGGATTATTGCTAGAAT
GGAATGGGGGCTCGTTGCTCATGTTGGAGTTCCTGATTTAGAAACCCGTGTTGCTATTTTACAACATAAGGCTGAACAAA
AAGGACTTAACATTCCTAACGAAATTGCTTTTTACATAGCAGACCACGTTTATGGAAACGTTCGTCAACTTGAAGGAGCG
ATTAATAAACTTACTGCATATTGTCTTTTATTTAATAAGCCTCTGACAGAAACCACTGTTCGGGATACTTTAAAGGAGCT
TTTCCGTACCCCATCTAAACAGAAAGTTTCTGTTGAGAGCATTTTAAAGAGTGTTGCTACAGTATTTCAGGTAAAAATTC
AGGATCTTAAGGGAACTTCTAGAGCTAAAAATGTGCCTTTAGCTAGACAAGTTGCCATGTATCTGGCTAAAACTCTAATT
ACCGATTCTCTTGTTGCGATTGGTGCCGCTTTTGGAAAAACTCACTCAACAGTTCTTTATGCATGCAAGACAATCGAACA
AAAAATAGAAAAAGACGCTCTGCTAAAAAATCAGATTAGCTTATGCAAGAATAATATAGCGATAGACTCACCACAACACT
TTGTGTAG

Upstream 100 bases:

>100_bases
GTATTGATTGCTTATCGATAGCAGTGCTACATTTGCGGTTTTCTCCAGGAGGAGGATACATTACCGAATCCTTAATTCCT
TAAGCGATAAAGAGAATGTG

Downstream 100 bases:

>100_bases
TCTTTTCGAGCTTTTTTATTTTACATCGACCGTCTTATGTGGGGAGAGAAATGTTCAAGAGACCTACGAAAAATTTTTTT
GATGGAGTCCAAACTTTGTA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLTCNDCSTWEQFVNYIKTRCSKTAFENWIAPIQVLEESREKIRLEIPNIFVQSYLLDNYKKDLCSFVPLDAEGNPALEF
VVSEIKRSSPQIAASVTKPAVEVSEENKDFQLKLNGAYRFDNFIEGPSNQFVKSAALGIAARPGRSYNPLFIHGGVGLGK
THLLHAVGHYVREHHKNLRIHCITTEAFINDLVHHLRVKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFETLI
HLSKQIVVTSDKPPGQLKLSERIIARMEWGLVAHVGVPDLETRVAILQHKAEQKGLNIPNEIAFYIADHVYGNVRQLEGA
INKLTAYCLLFNKPLTETTVRDTLKELFRTPSKQKVSVESILKSVATVFQVKIQDLKGTSRAKNVPLARQVAMYLAKTLI
TDSLVAIGAAFGKTHSTVLYACKTIEQKIEKDALLKNQISLCKNNIAIDSPQHFV

Sequences:

>Translated_455_residues
MLTCNDCSTWEQFVNYIKTRCSKTAFENWIAPIQVLEESREKIRLEIPNIFVQSYLLDNYKKDLCSFVPLDAEGNPALEF
VVSEIKRSSPQIAASVTKPAVEVSEENKDFQLKLNGAYRFDNFIEGPSNQFVKSAALGIAARPGRSYNPLFIHGGVGLGK
THLLHAVGHYVREHHKNLRIHCITTEAFINDLVHHLRVKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFETLI
HLSKQIVVTSDKPPGQLKLSERIIARMEWGLVAHVGVPDLETRVAILQHKAEQKGLNIPNEIAFYIADHVYGNVRQLEGA
INKLTAYCLLFNKPLTETTVRDTLKELFRTPSKQKVSVESILKSVATVFQVKIQDLKGTSRAKNVPLARQVAMYLAKTLI
TDSLVAIGAAFGKTHSTVLYACKTIEQKIEKDALLKNQISLCKNNIAIDSPQHFV
>Mature_455_residues
MLTCNDCSTWEQFVNYIKTRCSKTAFENWIAPIQVLEESREKIRLEIPNIFVQSYLLDNYKKDLCSFVPLDAEGNPALEF
VVSEIKRSSPQIAASVTKPAVEVSEENKDFQLKLNGAYRFDNFIEGPSNQFVKSAALGIAARPGRSYNPLFIHGGVGLGK
THLLHAVGHYVREHHKNLRIHCITTEAFINDLVHHLRVKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFETLI
HLSKQIVVTSDKPPGQLKLSERIIARMEWGLVAHVGVPDLETRVAILQHKAEQKGLNIPNEIAFYIADHVYGNVRQLEGA
INKLTAYCLLFNKPLTETTVRDTLKELFRTPSKQKVSVESILKSVATVFQVKIQDLKGTSRAKNVPLARQVAMYLAKTLI
TDSLVAIGAAFGKTHSTVLYACKTIEQKIEKDALLKNQISLCKNNIAIDSPQHFV

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=461, Percent_Identity=37.3101952277657, Blast_Score=305, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA2_CHLMU (Q9PKB9)

Other databases:

- EMBL:   AE002160
- PIR:   G81689
- RefSeq:   NP_296924.1
- ProteinModelPortal:   Q9PKB9
- GeneID:   1245907
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0547
- TIGR:   TC_0547
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- PhylomeDB:   Q9PKB9
- ProtClustDB:   PRK00149
- BioCyc:   CMUR243161:TC_0547-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51447; Mature: 51447

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCNDCSTWEQFVNYIKTRCSKTAFENWIAPIQVLEESREKIRLEIPNIFVQSYLLDNY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHH
KKDLCSFVPLDAEGNPALEFVVSEIKRSSPQIAASVTKPAVEVSEENKDFQLKLNGAYRF
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCEEEEECCCEEC
DNFIEGPSNQFVKSAALGIAARPGRSYNPLFIHGGVGLGKTHLLHAVGHYVREHHKNLRI
CHHCCCCCHHHHHHHHHCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
HCITTEAFINDLVHHLRVKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFETLI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HLSKQIVVTSDKPPGQLKLSERIIARMEWGLVAHVGVPDLETRVAILQHKAEQKGLNIPN
HHCCCEEEECCCCCCCEEHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCH
EIAFYIADHVYGNVRQLEGAINKLTAYCLLFNKPLTETTVRDTLKELFRTPSKQKVSVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ILKSVATVFQVKIQDLKGTSRAKNVPLARQVAMYLAKTLITDSLVAIGAAFGKTHSTVLY
HHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ACKTIEQKIEKDALLKNQISLCKNNIAIDSPQHFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLTCNDCSTWEQFVNYIKTRCSKTAFENWIAPIQVLEESREKIRLEIPNIFVQSYLLDNY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHH
KKDLCSFVPLDAEGNPALEFVVSEIKRSSPQIAASVTKPAVEVSEENKDFQLKLNGAYRF
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCEEEEECCCEEC
DNFIEGPSNQFVKSAALGIAARPGRSYNPLFIHGGVGLGKTHLLHAVGHYVREHHKNLRI
CHHCCCCCHHHHHHHHHCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
HCITTEAFINDLVHHLRVKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFETLI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HLSKQIVVTSDKPPGQLKLSERIIARMEWGLVAHVGVPDLETRVAILQHKAEQKGLNIPN
HHCCCEEEECCCCCCCEEHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCH
EIAFYIADHVYGNVRQLEGAINKLTAYCLLFNKPLTETTVRDTLKELFRTPSKQKVSVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ILKSVATVFQVKIQDLKGTSRAKNVPLARQVAMYLAKTLITDSLVAIGAAFGKTHSTVLY
HHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ACKTIEQKIEKDALLKNQISLCKNNIAIDSPQHFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935