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The map label for this gene is pfkA-1

Identifier: 15835095

GI number: 15835095

Start: 578639

End: 580300

Strand: Direct

Name: pfkA-1

Synonym: TC0477

Alternate gene names: 15835095

Gene position: 578639-580300 (Clockwise)

Preceding gene: 15835094

Following gene: 15835096

Centisome position: 53.93

GC content: 39.23

Gene sequence:

>1662_bases
ATGTCGTCTAGTAAACATTCTTCTCTTTGTCAAAAGACACCTCCTTTGTGCAGGGAGCTTCAAAAAGCTCCCGCACTCCT
CTTAACAGAGGACACTAGATTTAGTGCTCTTCTTAATGATCGTGTAGAGACTGTAGCGCAATTATTCCCATGTACCTATG
AATCCCCCTATCGCAAGTTTATTCCTAAATCCGATCTCCCTTTAGATATAGCTCCTCTTAAAGTTGGTGTTATGCTTTCT
GGAGGTCCTGCTCCTGGAGGGCATAATGTCATTTTAGGGTTGCTCCATAGCATTAAAAAATTACATCCTCATAGCAAACT
TTTAGGGTTCATTCGTAATGGTCATGGCCTACTCAATAATAATACAGTAGAGATTACAGAAGAGTTCATGAAAGAGTTTC
GTAACTCTGGAGGATTTAACTGTATTGGAACTGGCCGAACAAATATCATTACTGAAGAAAATAAGGCACGTTGCTTACAG
ACAGCGAATCAGCTTGATTTAGATGGTTTAGTCATCATCGGAGGAGATGGGTCAAATACAGCTACAGCGATTCTTGCTGA
ATACTTTGCAAAACATCGTGCCAAAACTGTGCTAATCGGTGTACCTAAAACTATAGATGGTGATTTACAACATCTCTTCT
TGGACTTAACTTTTGGTTTTGATACCGCTACCAAATTCTATTCATCGATTATCAGCAATATTTCTAGAGATGCGTTGTCT
TGTAAGGGGCATTATCACTTTATTAAACTGATGGGGCGATCTTCATCGCACATCACACTAGAATGTGCGCTACAAACTCA
CCCAAATATGGCTCTCATAGGAGAAGAGATAGCAGAAAAAGGCACTTCTCTTTCAGCCCTAATACAGGGAATTTGCGAGA
TGATATCTGATCGAGCCGCTATGGGGAAATATCATGGGGTTATTCTTATCCCAGAAGGGGTTATCGAGTTTATTCCCGAG
ATACAAACTCTTGTCAAAGAAATTGAATCCTTCCCCGAACAAACAGACCTTTCCCAAGCATTATCCCCCTCATCCCAACA
ACTTTTGCATCAATTTCCAGAAGATATTCGTCACCAGCTTCTATATAACCGCGATGCTCATGGGAATGTTTACGTATCTC
AAATTAGTGTCGACAAACTTCTTATTCATTTAGTTCAGAATCATCTGGAAACTCATTTCAAACATGTCCCTTTTAATGCC
ATTTCTCATTTTCTCGGTTATGAAGGACGCTCAGGTACCCCCACCCATTTTGATAACGTATACAGCTATAATTTAGGTTA
TGGTGCAGGTATCCTTGTCTTCAACCGCTGCAATGGGTACTTAACTACTATCGAGGGATTAACTAATCCTATTGAAAAAT
GGCGGCTACGAGCTTTGCCTATTGTACGTATGTTAACCACGAAAACAAATAAAGATGGGACCCTTTATCCGTTAATTAAG
AAAGGTTTAGTGGATATTTCTAGTCCTGTTTTTAATAAATTCTCTCTCTATCGCAAAATTTGGGCATTAGAAGATTCCTA
TCGTTTTGTAGGGCCTTTGCAAATACATTCTCCAGAAGATTCTCATTCTGATGATTTTCCCCCATTAATTTTGTTTTTAA
ATCATAATGAATGGCAAAAACGCTGTTCGATTTGCTTAGAAATCCCTGACCAGGATTATTAG

Upstream 100 bases:

>100_bases
TTTATTCTTAGCATAGTTAACCTAATTATTTGGTTAGGCTTAGGCAGTTGGTGGTGGTACTGTTTAGGATTAATTCGCTA
ACGTGTTCGAGACAGAACTT

Downstream 100 bases:

>100_bases
TTCCAATAGATATAACCGAAGTCCATCTTTTGCCATGAAACAACCTAGTTACTTTCCTCACTCAAAAAAAAACTTCGACA
TCCCTCTATTGGATAATGTC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSSSKHSSLCQKTPPLCRELQKAPALLLTEDTRFSALLNDRVETVAQLFPCTYESPYRKFIPKSDLPLDIAPLKVGVMLS
GGPAPGGHNVILGLLHSIKKLHPHSKLLGFIRNGHGLLNNNTVEITEEFMKEFRNSGGFNCIGTGRTNIITEENKARCLQ
TANQLDLDGLVIIGGDGSNTATAILAEYFAKHRAKTVLIGVPKTIDGDLQHLFLDLTFGFDTATKFYSSIISNISRDALS
CKGHYHFIKLMGRSSSHITLECALQTHPNMALIGEEIAEKGTSLSALIQGICEMISDRAAMGKYHGVILIPEGVIEFIPE
IQTLVKEIESFPEQTDLSQALSPSSQQLLHQFPEDIRHQLLYNRDAHGNVYVSQISVDKLLIHLVQNHLETHFKHVPFNA
ISHFLGYEGRSGTPTHFDNVYSYNLGYGAGILVFNRCNGYLTTIEGLTNPIEKWRLRALPIVRMLTTKTNKDGTLYPLIK
KGLVDISSPVFNKFSLYRKIWALEDSYRFVGPLQIHSPEDSHSDDFPPLILFLNHNEWQKRCSICLEIPDQDY

Sequences:

>Translated_553_residues
MSSSKHSSLCQKTPPLCRELQKAPALLLTEDTRFSALLNDRVETVAQLFPCTYESPYRKFIPKSDLPLDIAPLKVGVMLS
GGPAPGGHNVILGLLHSIKKLHPHSKLLGFIRNGHGLLNNNTVEITEEFMKEFRNSGGFNCIGTGRTNIITEENKARCLQ
TANQLDLDGLVIIGGDGSNTATAILAEYFAKHRAKTVLIGVPKTIDGDLQHLFLDLTFGFDTATKFYSSIISNISRDALS
CKGHYHFIKLMGRSSSHITLECALQTHPNMALIGEEIAEKGTSLSALIQGICEMISDRAAMGKYHGVILIPEGVIEFIPE
IQTLVKEIESFPEQTDLSQALSPSSQQLLHQFPEDIRHQLLYNRDAHGNVYVSQISVDKLLIHLVQNHLETHFKHVPFNA
ISHFLGYEGRSGTPTHFDNVYSYNLGYGAGILVFNRCNGYLTTIEGLTNPIEKWRLRALPIVRMLTTKTNKDGTLYPLIK
KGLVDISSPVFNKFSLYRKIWALEDSYRFVGPLQIHSPEDSHSDDFPPLILFLNHNEWQKRCSICLEIPDQDY
>Mature_552_residues
SSSKHSSLCQKTPPLCRELQKAPALLLTEDTRFSALLNDRVETVAQLFPCTYESPYRKFIPKSDLPLDIAPLKVGVMLSG
GPAPGGHNVILGLLHSIKKLHPHSKLLGFIRNGHGLLNNNTVEITEEFMKEFRNSGGFNCIGTGRTNIITEENKARCLQT
ANQLDLDGLVIIGGDGSNTATAILAEYFAKHRAKTVLIGVPKTIDGDLQHLFLDLTFGFDTATKFYSSIISNISRDALSC
KGHYHFIKLMGRSSSHITLECALQTHPNMALIGEEIAEKGTSLSALIQGICEMISDRAAMGKYHGVILIPEGVIEFIPEI
QTLVKEIESFPEQTDLSQALSPSSQQLLHQFPEDIRHQLLYNRDAHGNVYVSQISVDKLLIHLVQNHLETHFKHVPFNAI
SHFLGYEGRSGTPTHFDNVYSYNLGYGAGILVFNRCNGYLTTIEGLTNPIEKWRLRALPIVRMLTTKTNKDGTLYPLIKK
GLVDISSPVFNKFSLYRKIWALEDSYRFVGPLQIHSPEDSHSDDFPPLILFLNHNEWQKRCSICLEIPDQDY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=240, Percent_Identity=28.3333333333333, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61789; Mature: 61657

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKHSSLCQKTPPLCRELQKAPALLLTEDTRFSALLNDRVETVAQLFPCTYESPYRKF
CCCCCHHHHHHCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHC
IPKSDLPLDIAPLKVGVMLSGGPAPGGHNVILGLLHSIKKLHPHSKLLGFIRNGHGLLNN
CCCCCCCCEEECEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
NTVEITEEFMKEFRNSGGFNCIGTGRTNIITEENKARCLQTANQLDLDGLVIIGGDGSNT
CHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCH
ATAILAEYFAKHRAKTVLIGVPKTIDGDLQHLFLDLTFGFDTATKFYSSIISNISRDALS
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
CKGHYHFIKLMGRSSSHITLECALQTHPNMALIGEEIAEKGTSLSALIQGICEMISDRAA
CCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MGKYHGVILIPEGVIEFIPEIQTLVKEIESFPEQTDLSQALSPSSQQLLHQFPEDIRHQL
HCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCHHHHHHH
LYNRDAHGNVYVSQISVDKLLIHLVQNHLETHFKHVPFNAISHFLGYEGRSGTPTHFDNV
HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
YSYNLGYGAGILVFNRCNGYLTTIEGLTNPIEKWRLRALPIVRMLTTKTNKDGTLYPLIK
EEECCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KGLVDISSPVFNKFSLYRKIWALEDSYRFVGPLQIHSPEDSHSDDFPPLILFLNHNEWQK
HHHHHCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCCCCCCCEEEEECCCHHHH
RCSICLEIPDQDY
HCCEEEECCCCCC
>Mature Secondary Structure 
SSSKHSSLCQKTPPLCRELQKAPALLLTEDTRFSALLNDRVETVAQLFPCTYESPYRKF
CCCCHHHHHHCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHC
IPKSDLPLDIAPLKVGVMLSGGPAPGGHNVILGLLHSIKKLHPHSKLLGFIRNGHGLLNN
CCCCCCCCEEECEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
NTVEITEEFMKEFRNSGGFNCIGTGRTNIITEENKARCLQTANQLDLDGLVIIGGDGSNT
CHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCH
ATAILAEYFAKHRAKTVLIGVPKTIDGDLQHLFLDLTFGFDTATKFYSSIISNISRDALS
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
CKGHYHFIKLMGRSSSHITLECALQTHPNMALIGEEIAEKGTSLSALIQGICEMISDRAA
CCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MGKYHGVILIPEGVIEFIPEIQTLVKEIESFPEQTDLSQALSPSSQQLLHQFPEDIRHQL
HCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCHHHHHHH
LYNRDAHGNVYVSQISVDKLLIHLVQNHLETHFKHVPFNAISHFLGYEGRSGTPTHFDNV
HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
YSYNLGYGAGILVFNRCNGYLTTIEGLTNPIEKWRLRALPIVRMLTTKTNKDGTLYPLIK
EEECCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KGLVDISSPVFNKFSLYRKIWALEDSYRFVGPLQIHSPEDSHSDDFPPLILFLNHNEWQK
HHHHHCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCCCCCCCEEEEECCCHHHH
RCSICLEIPDQDY
HCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA