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The map label for this gene is tgt

Identifier: 15835083

GI number: 15835083

Start: 565281

End: 566399

Strand: Reverse

Name: tgt

Synonym: TC0465

Alternate gene names: 15835083

Gene position: 566399-565281 (Counterclockwise)

Preceding gene: 15835084

Following gene: 15835082

Centisome position: 52.79

GC content: 41.73

Gene sequence:

>1119_bases
GTGGCTTTGCGATTCGAGATTTTGCATCAATCGAAAAAATCTAGAGCTCGGGTAGGCCGAATAGAAACTGAGCATGGGTG
TATTGATACCCCCGCTTTTGTTCCTGTAGCTACAAATGGAGCGTTAAAAGGGGTGTTAGACCATAGTAATATCCCTCTGA
TGTTTTGCAATACATACCATCTTATTGTTCACCCAGGAGCAGAAGCTGTTGCTGCTATGGGCGGGTTACATCAATTTATT
GGTAGAAATGCCCCTATTATTACTGATTCTGGAGGGTTCCAAATTTTTAGTTTAGCTTACGGCTCTGTCGCTGAAGAGAT
CAAAAGTTGCGGGAAAAAGAAGGGTGAGAATAGCATTATTAAAATTAATGACGAGGGGGTTTGGTTTAAATCTTATCGGG
ATGGACGCAAGCTTTTTTTGTCTCCAGAAGTTTCTGTGCAAGCACAAAAGCATCTGGGGGCCGATATTATCATCCCTTTA
GATGAATTGCTCCCCTTTCATACAGATCCCGCATATTTTCAACAATCATCCCAGCGCACTTACGCTTGGGAAAAACGATC
TTTGGATTATCATCTAGCAAATCCCGGGTATCAATCCATGTATGGAGTGATTCATGGAGGAACTTTCCCTGATCAAAGAA
AACTGGGGTGTCAATTTGTAGAAGATTTGCCCTTTGATGGGTCAGCTATTGGCGGCAGTCTTGGAAAAAATCTTCGGGAC
ATTGTGGGAGTAGTCGATGTGACTACGGCGAATCTTTCTATAGAAAGGCCTCGACATTTATTGGGAATAGGAGATTTGCC
CTCTATTTGGGCAACCGTTGGGTTTGGGATAGACTCTTTTGATAGTTCTTATCCAACTAAAGCAGCGCGACATGGGATGA
TTCTGACATCACAGGGGTCTTTAAAAATTAACAATCAACGGTACGCTTCGGATTTGAATCCGATAGAACCAGGGTGTGCT
TGTCCCGCATGTTCACAAGGTATATCGCGAGCTTATTTAAGACATTTGTTTAAGGTGCATGAGCCAAATGCGGGTATCTG
GGCCTCCATTCATAATATGCATTATATGCAAAAAATAATGAGTAAAATTAGAGAAAAGATTTTAAATGATCAACTGTAA

Upstream 100 bases:

>100_bases
CTTTCTTCGGACTTTTTTTCTATCCCTTTTCTTTATCTCTCTTTAAAATAGTTCATGAATTTTGAAGAGTTTGGAATTTA
CTTAGAATAGAGGTTGGTTT

Downstream 100 bases:

>100_bases
ATTAATTTATTTAGTTTGTTGTTTATTTTTTAATTAAAAACTGTTAAAATAATCCCTCTTTTTCTTAAAAAATAAAAGAA
ATGAGGGTGGGGATTATGAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MALRFEILHQSKKSRARVGRIETEHGCIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYHLIVHPGAEAVAAMGGLHQFI
GRNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGENSIIKINDEGVWFKSYRDGRKLFLSPEVSVQAQKHLGADIIIPL
DELLPFHTDPAYFQQSSQRTYAWEKRSLDYHLANPGYQSMYGVIHGGTFPDQRKLGCQFVEDLPFDGSAIGGSLGKNLRD
IVGVVDVTTANLSIERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGSLKINNQRYASDLNPIEPGCA
CPACSQGISRAYLRHLFKVHEPNAGIWASIHNMHYMQKIMSKIREKILNDQL

Sequences:

>Translated_372_residues
MALRFEILHQSKKSRARVGRIETEHGCIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYHLIVHPGAEAVAAMGGLHQFI
GRNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGENSIIKINDEGVWFKSYRDGRKLFLSPEVSVQAQKHLGADIIIPL
DELLPFHTDPAYFQQSSQRTYAWEKRSLDYHLANPGYQSMYGVIHGGTFPDQRKLGCQFVEDLPFDGSAIGGSLGKNLRD
IVGVVDVTTANLSIERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGSLKINNQRYASDLNPIEPGCA
CPACSQGISRAYLRHLFKVHEPNAGIWASIHNMHYMQKIMSKIREKILNDQL
>Mature_371_residues
ALRFEILHQSKKSRARVGRIETEHGCIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYHLIVHPGAEAVAAMGGLHQFIG
RNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGENSIIKINDEGVWFKSYRDGRKLFLSPEVSVQAQKHLGADIIIPLD
ELLPFHTDPAYFQQSSQRTYAWEKRSLDYHLANPGYQSMYGVIHGGTFPDQRKLGCQFVEDLPFDGSAIGGSLGKNLRDI
VGVVDVTTANLSIERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGSLKINNQRYASDLNPIEPGCAC
PACSQGISRAYLRHLFKVHEPNAGIWASIHNMHYMQKIMSKIREKILNDQL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=373, Percent_Identity=32.7077747989276, Blast_Score=209, Evalue=2e-54,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=33.6, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17544680, Length=376, Percent_Identity=30.8510638297872, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI19920510, Length=375, Percent_Identity=32.8, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CHLMU (Q9PKK0)

Other databases:

- EMBL:   AE002160
- PIR:   A81699
- RefSeq:   NP_296842.1
- ProteinModelPortal:   Q9PKK0
- SMR:   Q9PKK0
- GeneID:   1245820
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0465
- TIGR:   TC_0465
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK01008
- BioCyc:   CMUR243161:TC_0465-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41016; Mature: 40885

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRFEILHQSKKSRARVGRIETEHGCIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYH
CCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEECEEE
LIVHPGAEAVAAMGGLHQFIGRNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGENSII
EEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
KINDEGVWFKSYRDGRKLFLSPEVSVQAQKHLGADIIIPLDELLPFHTDPAYFQQSSQRT
EECCCCEEEECCCCCCEEEECCCCCCHHHHHCCCEEEEEHHHHCCCCCCHHHHHCCCCCC
YAWEKRSLDYHLANPGYQSMYGVIHGGTFPDQRKLGCQFVEDLPFDGSAIGGSLGKNLRD
EEEHHCCCCEEECCCCHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCHHHH
IVGVVDVTTANLSIERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGS
HHHHHEEEECCEEECCCHHEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCEEEECCCC
LKINNQRYASDLNPIEPGCACPACSQGISRAYLRHLFKVHEPNAGIWASIHNMHYMQKIM
EEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SKIREKILNDQL
HHHHHHHHCCCC
>Mature Secondary Structure 
ALRFEILHQSKKSRARVGRIETEHGCIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYH
CEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEECEEE
LIVHPGAEAVAAMGGLHQFIGRNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGENSII
EEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
KINDEGVWFKSYRDGRKLFLSPEVSVQAQKHLGADIIIPLDELLPFHTDPAYFQQSSQRT
EECCCCEEEECCCCCCEEEECCCCCCHHHHHCCCEEEEEHHHHCCCCCCHHHHHCCCCCC
YAWEKRSLDYHLANPGYQSMYGVIHGGTFPDQRKLGCQFVEDLPFDGSAIGGSLGKNLRD
EEEHHCCCCEEECCCCHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCHHHH
IVGVVDVTTANLSIERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGS
HHHHHEEEECCEEECCCHHEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCEEEECCCC
LKINNQRYASDLNPIEPGCACPACSQGISRAYLRHLFKVHEPNAGIWASIHNMHYMQKIM
EEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SKIREKILNDQL
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935