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The map label for this gene is sucB

Identifier: 15834945

GI number: 15834945

Start: 384736

End: 385830

Strand: Direct

Name: sucB

Synonym: TC0325

Alternate gene names: 15834945

Gene position: 384736-385830 (Clockwise)

Preceding gene: 15834944

Following gene: 15834950

Centisome position: 35.86

GC content: 41.1

Gene sequence:

>1095_bases
ATGAGTATAGAGGTTCGTATCCCCAATATCGCAGAGTCTATTAGTGAAGTCACGGTATCTGCATTGCTGGTTGCATCCGG
AGATTTTGTGCAAGAGAATCAGGGGATTCTAGAGATAGAAAGTGATAAGGTGAATCAATTGATTTATGCTCCCTGCTCAG
GAAGAGTAGAATGGAGTGTTTCCGTAGGTGATACGGTGGCTGTTGGAAGCGTCGTGGGCACCATCTGCAAATTGGAAAAC
CAGGATACTCCATCTATTCATGAGCAAATGCCATTCAGTCTTGTGGAGCAAGAAAGCGATGCACAGATTATCTCCTTCCC
TTCATCAGTACGACAGGATCCTCCTGCAGAAGGGAAAACATTTGTTCCTCTGAAGGAGATAGAACGAGATTCTTCCGATA
AGAAAGAATCTCGAGAATCTATGAGTGCTATTCGTAAGACGATTTCTCGTAGATTAGTTCAAGCTTTGCATGATTCGGCA
ATGTTAACTACGTTTAACGAAGTATGTATGGGGCCGATTATTGCTTTGCGAAAAGAAAAACAAGAAGCTTTTGTTTCCAA
GTATGGTGTTAAGCTTGGTTTTATGTCCTTTTTTGTTAAAGCAGTAGTAGATTCTTTGAAAAAATACCCTAGGGTTAATG
CTTATATCAATGGGAATGAAATCGTTTATAGACATTACTACGATATTTCTATTGCTGTAGGGACAGATCGTGGATTGGTC
GTTCCAGTGATTCGTAATTGTGACCGGTTATCATGTGGAGAAATTGAGGTGCAACTTGCAGATTTAGCTTCGCGGGCTAG
AGATGGGAAGCTTGCTATTCATGAGTTGGAAGGAGGGAGCTTCACAATTACTAACGGAGGGGTTTATGGTTCTCTTTTAT
CAACACCTATTATTAATCCTCCTCAGGTTGGGATCCTTGGAATGCACAAGATAGAGAAACGTCCTGTAGTTAAAGATGAT
TCAATCATTATAGCGGATATGATGTATGTGGCTATGAGTTATGATCATCGGATTATAGATGGAAAAGAGGCCGTAGGGTT
CCTTGTCAACGTTAAGGAATTATTGGAGCAGCCGGAGCTTTTATTGACTATCTAG

Upstream 100 bases:

>100_bases
TCCTAGAAGTAGTTCGACTGCAACAGGATCTGCAAATTTGAGCCAAAAAGAATTATCCACATTAATGGAAACATTGTTTT
CTATAGGTAGAGAGTAGAGC

Downstream 100 bases:

>100_bases
TCTAGAAGAATATCTGAATCAAAGGGATAAGGCTATCTAAGAAGAAGCAATACTGCAGTAACATTGTTCGCGCGAGGACG
AATTGCTGCAGGATCATTTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSIEVRIPNIAESISEVTVSALLVASGDFVQENQGILEIESDKVNQLIYAPCSGRVEWSVSVGDTVAVGSVVGTICKLEN
QDTPSIHEQMPFSLVEQESDAQIISFPSSVRQDPPAEGKTFVPLKEIERDSSDKKESRESMSAIRKTISRRLVQALHDSA
MLTTFNEVCMGPIIALRKEKQEAFVSKYGVKLGFMSFFVKAVVDSLKKYPRVNAYINGNEIVYRHYYDISIAVGTDRGLV
VPVIRNCDRLSCGEIEVQLADLASRARDGKLAIHELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVKDD
SIIIADMMYVAMSYDHRIIDGKEAVGFLVNVKELLEQPELLLTI

Sequences:

>Translated_364_residues
MSIEVRIPNIAESISEVTVSALLVASGDFVQENQGILEIESDKVNQLIYAPCSGRVEWSVSVGDTVAVGSVVGTICKLEN
QDTPSIHEQMPFSLVEQESDAQIISFPSSVRQDPPAEGKTFVPLKEIERDSSDKKESRESMSAIRKTISRRLVQALHDSA
MLTTFNEVCMGPIIALRKEKQEAFVSKYGVKLGFMSFFVKAVVDSLKKYPRVNAYINGNEIVYRHYYDISIAVGTDRGLV
VPVIRNCDRLSCGEIEVQLADLASRARDGKLAIHELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVKDD
SIIIADMMYVAMSYDHRIIDGKEAVGFLVNVKELLEQPELLLTI
>Mature_363_residues
SIEVRIPNIAESISEVTVSALLVASGDFVQENQGILEIESDKVNQLIYAPCSGRVEWSVSVGDTVAVGSVVGTICKLENQ
DTPSIHEQMPFSLVEQESDAQIISFPSSVRQDPPAEGKTFVPLKEIERDSSDKKESRESMSAIRKTISRRLVQALHDSAM
LTTFNEVCMGPIIALRKEKQEAFVSKYGVKLGFMSFFVKAVVDSLKKYPRVNAYINGNEIVYRHYYDISIAVGTDRGLVV
PVIRNCDRLSCGEIEVQLADLASRARDGKLAIHELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVKDDS
IIIADMMYVAMSYDHRIIDGKEAVGFLVNVKELLEQPELLLTI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=242, Percent_Identity=50.8264462809917, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI31711992, Length=224, Percent_Identity=33.4821428571429, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI203098816, Length=224, Percent_Identity=32.5892857142857, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI203098753, Length=224, Percent_Identity=32.5892857142857, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI110671329, Length=232, Percent_Identity=31.8965517241379, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=36.7088607594937, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=42.2885572139304, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1786305, Length=212, Percent_Identity=33.4905660377358, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=398, Percent_Identity=39.6984924623116, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17560088, Length=256, Percent_Identity=32.03125, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537937, Length=261, Percent_Identity=28.735632183908, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=223, Percent_Identity=33.6322869955157, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=397, Percent_Identity=37.0277078085642, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324258, Length=229, Percent_Identity=31.4410480349345, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=48.995983935743, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18859875, Length=229, Percent_Identity=34.4978165938865, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20129315, Length=224, Percent_Identity=30.8035714285714, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24582497, Length=224, Percent_Identity=30.8035714285714, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 40107; Mature: 39975

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVRIPNIAESISEVTVSALLVASGDFVQENQGILEIESDKVNQLIYAPCSGRVEWSV
CEEEEECCHHHHHHHHHHHHHHHEECCCCEECCCCEEEECCCCCCEEEEECCCCEEEEEE
SVGDTVAVGSVVGTICKLENQDTPSIHEQMPFSLVEQESDAQIISFPSSVRQDPPAEGKT
ECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCCCEEEECCHHHCCCCCCCCCE
FVPLKEIERDSSDKKESRESMSAIRKTISRRLVQALHDSAMLTTFNEVCMGPIIALRKEK
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QEAFVSKYGVKLGFMSFFVKAVVDSLKKYPRVNAYINGNEIVYRHYYDISIAVGTDRGLV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEECCCCEE
VPVIRNCDRLSCGEIEVQLADLASRARDGKLAIHELEGGSFTITNGGVYGSLLSTPIINP
EHHHCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCEEEEECCCEEHHHHHCCCCCC
PQVGILGMHKIEKRPVVKDDSIIIADMMYVAMSYDHRIIDGKEAVGFLVNVKELLEQPEL
CCCCEEEHHHHCCCCCCCCCCEEHHHHHHHHHCCCCEEECCHHHHHHHEEHHHHHCCCCE
LLTI
EEEC
>Mature Secondary Structure 
SIEVRIPNIAESISEVTVSALLVASGDFVQENQGILEIESDKVNQLIYAPCSGRVEWSV
EEEEECCHHHHHHHHHHHHHHHEECCCCEECCCCEEEECCCCCCEEEEECCCCEEEEEE
SVGDTVAVGSVVGTICKLENQDTPSIHEQMPFSLVEQESDAQIISFPSSVRQDPPAEGKT
ECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCCCEEEECCHHHCCCCCCCCCE
FVPLKEIERDSSDKKESRESMSAIRKTISRRLVQALHDSAMLTTFNEVCMGPIIALRKEK
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QEAFVSKYGVKLGFMSFFVKAVVDSLKKYPRVNAYINGNEIVYRHYYDISIAVGTDRGLV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEECCCCEE
VPVIRNCDRLSCGEIEVQLADLASRARDGKLAIHELEGGSFTITNGGVYGSLLSTPIINP
EHHHCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCEEEEECCCEEHHHHHCCCCCC
PQVGILGMHKIEKRPVVKDDSIIIADMMYVAMSYDHRIIDGKEAVGFLVNVKELLEQPEL
CCCCEEEHHHHCCCCCCCCCCEEHHHHHHHHHCCCCEEECCHHHHHHHEEHHHHHCCCCE
LLTI
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]