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The map label for this gene is rnhB

Identifier: 15834918

GI number: 15834918

Start: 355302

End: 355955

Strand: Direct

Name: rnhB

Synonym: TC0298

Alternate gene names: 15834918

Gene position: 355302-355955 (Clockwise)

Preceding gene: 15834917

Following gene: 15834919

Centisome position: 33.11

GC content: 43.73

Gene sequence:

>654_bases
ATGAAATCAATCGTTGAGCAGGAGCTGCTTTTTAGAGAGAAGAGTGTTTTTGAGGATCAAGCTATCAAACAAGGATACTC
ACGAATTGCTGGTGTAGATGAGGCTGGGAGGGGGCCTCTTGCGGGTCCTGTCGTTGCTGGGGCTTGCATTCTGCCTGGAG
GGAAGCTTTTTTTAGGTATTGATGACAGTAAGAAGTTATCCCCTAAGCAGAGACGGTATTTGTATGAACTTTTGCTTGAA
GATCCCGAAGTCACTTGTGGGGTCGGAGTTGTCTCTGTTGAGCGAATAGATGAGATCAATATTTTAGAGGCTACCAAAGA
AGCTATGGTTCAGGCCATAGCTTCTTTGCGGAGCACTCCTGATTTTTTATTGGTTGACGGGTTGTTTTTGCCGCATGAGA
TCCCCTGTCTTAAAATTATAAAAGGAGATTCTCGTTCCGTGTCAATAGCTGCCGCATCCATAATAGCCAAAGAATATCGT
GATGAATTGATGCGGAAGCTTCATTCTGAATATCCTGAGTATGGGTTTGACAAACATAAGGGGTATGGAACGGTTGCGCA
TTTGCAAGCTTTAAAACAGTTTGGCCCTTGCGTATATCACAGAAAGAGTTTCTCTCCTGTAAAAGAGAGTATCCGAGAGG
GAATATGTCAGTAA

Upstream 100 bases:

>100_bases
TTAAAGAGCTTATTGGTCCTCGGGCTGCTAAGAAATAGTAAAAGAAAGAGAGCAGCATAAGCCTCTTTCTGCTGTTATCA
ACGTTAAGAGACCTCTTTAT

Downstream 100 bases:

>100_bases
AGGTTGCTTCCCCTTTTTCTCCAGATGGGGTCCAATGCCTTCCTAAACTTTTTACTATCAGTGCTCCTGCTGGAGCGGGG
AAGACAACTTTAGTTCATAT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKSIVEQELLFREKSVFEDQAIKQGYSRIAGVDEAGRGPLAGPVVAGACILPGGKLFLGIDDSKKLSPKQRRYLYELLLE
DPEVTCGVGVVSVERIDEINILEATKEAMVQAIASLRSTPDFLLVDGLFLPHEIPCLKIIKGDSRSVSIAAASIIAKEYR
DELMRKLHSEYPEYGFDKHKGYGTVAHLQALKQFGPCVYHRKSFSPVKESIREGICQ

Sequences:

>Translated_217_residues
MKSIVEQELLFREKSVFEDQAIKQGYSRIAGVDEAGRGPLAGPVVAGACILPGGKLFLGIDDSKKLSPKQRRYLYELLLE
DPEVTCGVGVVSVERIDEINILEATKEAMVQAIASLRSTPDFLLVDGLFLPHEIPCLKIIKGDSRSVSIAAASIIAKEYR
DELMRKLHSEYPEYGFDKHKGYGTVAHLQALKQFGPCVYHRKSFSPVKESIREGICQ
>Mature_217_residues
MKSIVEQELLFREKSVFEDQAIKQGYSRIAGVDEAGRGPLAGPVVAGACILPGGKLFLGIDDSKKLSPKQRRYLYELLLE
DPEVTCGVGVVSVERIDEINILEATKEAMVQAIASLRSTPDFLLVDGLFLPHEIPCLKIIKGDSRSVSIAAASIIAKEYR
DELMRKLHSEYPEYGFDKHKGYGTVAHLQALKQFGPCVYHRKSFSPVKESIREGICQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=46.195652173913, Blast_Score=157, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CHLMU (Q9PL10)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_296677.1
- ProteinModelPortal:   Q9PL10
- SMR:   Q9PL10
- GeneID:   1246468
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0298
- TIGR:   TC_0298
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q9PL10
- ProtClustDB:   PRK00015
- BioCyc:   CMUR243161:TC_0298-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24037; Mature: 24037

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIVEQELLFREKSVFEDQAIKQGYSRIAGVDEAGRGPLAGPVVAGACILPGGKLFLGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEE
DDSKKLSPKQRRYLYELLLEDPEVTCGVGVVSVERIDEINILEATKEAMVQAIASLRSTP
CCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DFLLVDGLFLPHEIPCLKIIKGDSRSVSIAAASIIAKEYRDELMRKLHSEYPEYGFDKHK
CEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYGTVAHLQALKQFGPCVYHRKSFSPVKESIREGICQ
CCCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKSIVEQELLFREKSVFEDQAIKQGYSRIAGVDEAGRGPLAGPVVAGACILPGGKLFLGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEE
DDSKKLSPKQRRYLYELLLEDPEVTCGVGVVSVERIDEINILEATKEAMVQAIASLRSTP
CCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DFLLVDGLFLPHEIPCLKIIKGDSRSVSIAAASIIAKEYRDELMRKLHSEYPEYGFDKHK
CEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYGTVAHLQALKQFGPCVYHRKSFSPVKESIREGICQ
CCCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935