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The map label for this gene is pth

Identifier: 15834803

GI number: 15834803

Start: 217325

End: 217864

Strand: Direct

Name: pth

Synonym: TC0183

Alternate gene names: 15834803

Gene position: 217325-217864 (Clockwise)

Preceding gene: 15834802

Following gene: 15834804

Centisome position: 20.25

GC content: 41.85

Gene sequence:

>540_bases
ATGGTGAAGCTGGTTGTTGGTATAGGGAATCCTGGAAGGCAATATGTCTGGACAAGACATAATATAGGGTTCCTTTTTCT
AGACATGCTGGCATCTCGTTTCTCTGGGGCTTTTCGTGAAGCCCCTCGTCTCTTTTCTTCGTTTATGAAGGTAGAAACTT
CCTGCGGAGTTATTGTTCTTATTAAACCCTCGACTTATGTGAATCTTACTGGCAAGGCTGTTTTGGCTGCCAAGAGGTTT
TTCGGCGTTTCTGTGGAAGGTATTTTGATTGTAGCCGATGATATCAATCGAGAGTTCGGATCTATACGTTTCCGACAAGA
CTGTGGTGCCGGTGGGCATAACGGGCTTAAAAATACCACACAAGTTCTGCAATCTAATCATTATTGGCAATTGCGTCTTG
GAGTTGGCAGGCCTTCAAATCCGGAGTCGGAGGGGGTTGCTGACTATGTGTTATCCAATTTTTCTTTTAATGAAAGAAAA
AGTTTAAATGGTTTTTTTGAAAAAGGAATAGAAGAAATTTCCCCTTGGTTAGGCTTTTAA

Upstream 100 bases:

>100_bases
CAGACTTTAAAATTATCGGATATCCGTATTCCTGAGGGAATTAAACCTGTTACTCCGCTTAAAGAAGTTGCTGTAACAGT
GGCTCGACGATAAGATTATC

Downstream 100 bases:

>100_bases
TTTAAAAGGGATTTACTGTTCACTTTTTGAAAAAAAGTTCCTGAAAAATAATCAGTTTCGATAACATGGAAGCGCTTCTT
ATAGAGTAGCTATCCATAGT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MVKLVVGIGNPGRQYVWTRHNIGFLFLDMLASRFSGAFREAPRLFSSFMKVETSCGVIVLIKPSTYVNLTGKAVLAAKRF
FGVSVEGILIVADDINREFGSIRFRQDCGAGGHNGLKNTTQVLQSNHYWQLRLGVGRPSNPESEGVADYVLSNFSFNERK
SLNGFFEKGIEEISPWLGF

Sequences:

>Translated_179_residues
MVKLVVGIGNPGRQYVWTRHNIGFLFLDMLASRFSGAFREAPRLFSSFMKVETSCGVIVLIKPSTYVNLTGKAVLAAKRF
FGVSVEGILIVADDINREFGSIRFRQDCGAGGHNGLKNTTQVLQSNHYWQLRLGVGRPSNPESEGVADYVLSNFSFNERK
SLNGFFEKGIEEISPWLGF
>Mature_179_residues
MVKLVVGIGNPGRQYVWTRHNIGFLFLDMLASRFSGAFREAPRLFSSFMKVETSCGVIVLIKPSTYVNLTGKAVLAAKRF
FGVSVEGILIVADDINREFGSIRFRQDCGAGGHNGLKNTTQVLQSNHYWQLRLGVGRPSNPESEGVADYVLSNFSFNERK
SLNGFFEKGIEEISPWLGF

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=29.6511627906977, Blast_Score=91, Evalue=6e-19,
Organism=Escherichia coli, GI1787455, Length=176, Percent_Identity=34.0909090909091, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_CHLMU (P49607)

Other databases:

- EMBL:   U31570
- EMBL:   AE002160
- EMBL:   M62820
- PIR:   C37840
- PIR:   C81732
- RefSeq:   NP_296562.1
- ProteinModelPortal:   P49607
- SMR:   P49607
- GeneID:   1246309
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0183
- TIGR:   TC_0183
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- BioCyc:   CMUR243161:TC_0183-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 19915; Mature: 19915

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLVVGIGNPGRQYVWTRHNIGFLFLDMLASRFSGAFREAPRLFSSFMKVETSCGVIVL
CEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEE
IKPSTYVNLTGKAVLAAKRFFGVSVEGILIVADDINREFGSIRFRQDCGAGGHNGLKNTT
ECCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCHHCEEEECCCCCCCCCCCHHHH
QVLQSNHYWQLRLGVGRPSNPESEGVADYVLSNFSFNERKSLNGFFEKGIEEISPWLGF
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKLVVGIGNPGRQYVWTRHNIGFLFLDMLASRFSGAFREAPRLFSSFMKVETSCGVIVL
CEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEE
IKPSTYVNLTGKAVLAAKRFFGVSVEGILIVADDINREFGSIRFRQDCGAGGHNGLKNTT
ECCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCHHCEEEECCCCCCCCCCCHHHH
QVLQSNHYWQLRLGVGRPSNPESEGVADYVLSNFSFNERKSLNGFFEKGIEEISPWLGF
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635758; 10684935; 2254267