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Identifier: 15834702

GI number: 15834702

Start: 89637

End: 90869

Strand: Reverse

Name: Not Available

Synonym: TC0077

Alternate gene names: 15834702

Gene position: 90869-89637 (Counterclockwise)

Preceding gene: 15834703

Following gene: 15834701

Centisome position: 8.47

GC content: 42.82

Gene sequence:

>1233_bases
ATGACCCAGTCCACTTTGGAAACTGCGAAAAAAATTGTTCAAAAATTACGCAATGCTGGGTATCAGGCGTATTTTGTTGG
TGGGAGTGTCCGAGATATGCTCTTGGACCGCCCCATTGAAGAGGTGGATATAGCAACTAACGCCTCCCCGACAATCGTGA
CTACTATCTTCCCCGATACTCTTTCCATTGGAGCTGCTTTCGGTATTATTGCTGTAAAAGAAAATGGACAACTGTTCGAA
GTGGCAACCTTTCGCAGTGATGAAGATTACGCAGATGGTCGTCACCCCAATCGCGTGATTTTTGCTTCTATGAAGGACGA
TGCCATTCGAAGGGATTTCACAATCAATGGCATGTACTATGACCCCTTTGAAGAAAAACTTTTTGATTTAGTAGAGGGAA
GGGCTGACCTAGAAAGAAAAGTTGTTCGAGCAATTGGACATCCTAAACAAAGATTTTTAGAAGATAAGCTCCGTATCCTT
AGAGCTATTCGCTTTGCCGCGACATTGGGCTTTTCTTTAGACCCGAAAACAGAGATCGCTATTATCAAAGAGGCTCCATC
TTTACCGACCTCGGTCTCCCCAGAAAGAATCTGGCAAGAGCTCAAAAAAATGCTTCGCAAAGCACCTTATGAGTCTCTTC
AATTACTTATTAAGCTTAAGGTTCTCCCAATCATCTTCCCAGAGTTAAAGGCCTCCCCTAGTGGATTTTTACGTTCTACT
ATTGCTTTTGCTCAAAGAATTCGGGATCCGCACATTCCAGAGATCGCTTTTTTACTCCCTCTCTTCCAGGAAGTAAAACA
GGAAGAGGCTATAGCAGCCTTCTCTCGCTTAAAAGTCTCCAACAAAGAGCTTGGGTTACTAGAAGATTGGTATGAAGCTT
TGCCTCAGTTTCGAGCTAAGCAGAATAATAAAATCTTTTGGGCACATTTTTTTGCTTCACAAACCTCCCCCCTACTCCTT
TCGCTCTTCTTGTCACTACAGCGTACACCTCATGAGCAGGAGTTGTTTATTTCTCGAGTACACCACTTGAAAACTCGTCT
TGCCTCATTCATAGAAAGGATTCAAACCGGCTCTCCTCTAGTTTCTGCACCAGACTTAATGGCAAGAGGAATTGCTCCTG
GTCGGTTACTAGGAGAGCTCCTCAAAGAGGCTGAAGCCTTATCCATAGAACATGAGTGCTCTGACAAAGAAAAAATTTTA
TCTCTGTTAAAAGATAAAGGTTTTTGGAAATAA

Upstream 100 bases:

>100_bases
TGGAATCTATTCGCATAGAGGAAGACACTATTACGCAAAACAAATCTCCTGTAATTATTCAAAGATCTCCAGAGGCTATT
GCTTAACTTTTAGGAATTGC

Downstream 100 bases:

>100_bases
CCTCTCCTTTCGATAAGGCTCTCTTAAATAGTTAATTTTATTTCTTTAGATTTAATTTTTTACTCCTTATCATTTCTTCC
TAATTATTCAATTCTGGATT

Product: poly(A) polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTQSTLETAKKIVQKLRNAGYQAYFVGGSVRDMLLDRPIEEVDIATNASPTIVTTIFPDTLSIGAAFGIIAVKENGQLFE
VATFRSDEDYADGRHPNRVIFASMKDDAIRRDFTINGMYYDPFEEKLFDLVEGRADLERKVVRAIGHPKQRFLEDKLRIL
RAIRFAATLGFSLDPKTEIAIIKEAPSLPTSVSPERIWQELKKMLRKAPYESLQLLIKLKVLPIIFPELKASPSGFLRST
IAFAQRIRDPHIPEIAFLLPLFQEVKQEEAIAAFSRLKVSNKELGLLEDWYEALPQFRAKQNNKIFWAHFFASQTSPLLL
SLFLSLQRTPHEQELFISRVHHLKTRLASFIERIQTGSPLVSAPDLMARGIAPGRLLGELLKEAEALSIEHECSDKEKIL
SLLKDKGFWK

Sequences:

>Translated_410_residues
MTQSTLETAKKIVQKLRNAGYQAYFVGGSVRDMLLDRPIEEVDIATNASPTIVTTIFPDTLSIGAAFGIIAVKENGQLFE
VATFRSDEDYADGRHPNRVIFASMKDDAIRRDFTINGMYYDPFEEKLFDLVEGRADLERKVVRAIGHPKQRFLEDKLRIL
RAIRFAATLGFSLDPKTEIAIIKEAPSLPTSVSPERIWQELKKMLRKAPYESLQLLIKLKVLPIIFPELKASPSGFLRST
IAFAQRIRDPHIPEIAFLLPLFQEVKQEEAIAAFSRLKVSNKELGLLEDWYEALPQFRAKQNNKIFWAHFFASQTSPLLL
SLFLSLQRTPHEQELFISRVHHLKTRLASFIERIQTGSPLVSAPDLMARGIAPGRLLGELLKEAEALSIEHECSDKEKIL
SLLKDKGFWK
>Mature_409_residues
TQSTLETAKKIVQKLRNAGYQAYFVGGSVRDMLLDRPIEEVDIATNASPTIVTTIFPDTLSIGAAFGIIAVKENGQLFEV
ATFRSDEDYADGRHPNRVIFASMKDDAIRRDFTINGMYYDPFEEKLFDLVEGRADLERKVVRAIGHPKQRFLEDKLRILR
AIRFAATLGFSLDPKTEIAIIKEAPSLPTSVSPERIWQELKKMLRKAPYESLQLLIKLKVLPIIFPELKASPSGFLRSTI
AFAQRIRDPHIPEIAFLLPLFQEVKQEEAIAAFSRLKVSNKELGLLEDWYEALPQFRAKQNNKIFWAHFFASQTSPLLLS
LFLSLQRTPHEQELFISRVHHLKTRLASFIERIQTGSPLVSAPDLMARGIAPGRLLGELLKEAEALSIEHECSDKEKILS
LLKDKGFWK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=228, Percent_Identity=36.4035087719298, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI87081691, Length=238, Percent_Identity=36.5546218487395, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=30.7339449541284, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=193, Percent_Identity=32.6424870466321, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=226, Percent_Identity=29.646017699115, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281360112, Length=284, Percent_Identity=31.3380281690141, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357337, Length=284, Percent_Identity=31.3380281690141, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46588; Mature: 46456

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSTLETAKKIVQKLRNAGYQAYFVGGSVRDMLLDRPIEEVDIATNASPTIVTTIFPDT
CCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCH
LSIGAAFGIIAVKENGQLFEVATFRSDEDYADGRHPNRVIFASMKDDAIRRDFTINGMYY
HHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHCCEEECCEEE
DPFEEKLFDLVEGRADLERKVVRAIGHPKQRFLEDKLRILRAIRFAATLGFSLDPKTEIA
CCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IIKEAPSLPTSVSPERIWQELKKMLRKAPYESLQLLIKLKVLPIIFPELKASPSGFLRST
EEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IAFAQRIRDPHIPEIAFLLPLFQEVKQEEAIAAFSRLKVSNKELGLLEDWYEALPQFRAK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
QNNKIFWAHFFASQTSPLLLSLFLSLQRTPHEQELFISRVHHLKTRLASFIERIQTGSPL
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAPDLMARGIAPGRLLGELLKEAEALSIEHECSDKEKILSLLKDKGFWK
CCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQSTLETAKKIVQKLRNAGYQAYFVGGSVRDMLLDRPIEEVDIATNASPTIVTTIFPDT
CCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCH
LSIGAAFGIIAVKENGQLFEVATFRSDEDYADGRHPNRVIFASMKDDAIRRDFTINGMYY
HHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHCCEEECCEEE
DPFEEKLFDLVEGRADLERKVVRAIGHPKQRFLEDKLRILRAIRFAATLGFSLDPKTEIA
CCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IIKEAPSLPTSVSPERIWQELKKMLRKAPYESLQLLIKLKVLPIIFPELKASPSGFLRST
EEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IAFAQRIRDPHIPEIAFLLPLFQEVKQEEAIAAFSRLKVSNKELGLLEDWYEALPQFRAK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
QNNKIFWAHFFASQTSPLLLSLFLSLQRTPHEQELFISRVHHLKTRLASFIERIQTGSPL
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAPDLMARGIAPGRLLGELLKEAEALSIEHECSDKEKILSLLKDKGFWK
CCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA