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The map label for this gene is pgk

Identifier: 15834690

GI number: 15834690

Start: 74721

End: 75932

Strand: Direct

Name: pgk

Synonym: TC0065

Alternate gene names: 15834690

Gene position: 74721-75932 (Clockwise)

Preceding gene: 15834689

Following gene: 15834691

Centisome position: 6.96

GC content: 44.55

Gene sequence:

>1212_bases
ATGGATAAACTATCAATAAAAGACCTTTCCCCAGAAGGGAAAAAGGTACTAGTTCGTGTAGATTTTAATGTTCCTATTAA
GGACGGAAAAATTTTAGATGATGTCCGTATTCGTAGTGCTATGCCTACGATCAATTACCTATTGAAGAGAGATGCTGCGA
TCATTTTAGTCAGTCATTTAGGGCGTCCTAAGGGAGATATGTTCGAAGAAGCGTATTCGTTAGCTCCTATTGTGCCTGTA
TTAGAAGGATATTTGGGGCATCATGTGCCTCTATCTCCAGATTGTGTTGGAGAAGTTGCACGGCAGGCTGTTGCGCAGTT
GTCTCCTGGAAGAGTGCTTCTTCTTGAAAATGTACGTTTTCATAGAGGGGAAGAGCATCCTGAGGAGGATCCTAGTTTTG
CTGTTGAGCTTGCTGCTTACGCCGATTTTTATGTAAATGATGCCTTCGGAACCTCTCATCGGAAGCATGCTTCTGTGTAT
CGAGTTCCTCAAATGTTCCCCGATCGTGCTGCTGCGGGATTTCTAATGGAAAAAGAGCTTGAATTTTTGGGACAGCATTT
ATTAGTGGAGCCTAAGCGTCCCTTTACGGCCATCCTAGGTGGTGCTAAGGTATCTTCAAAAATAGGGGTAATCGAAGCTT
TACTCTCGAGAGTTGATAATTTAGTGTTAGCTGGAGGTATGGGGTATACTTTTCTAAAAGCTATGAATCAGCAAGTTGGG
AATTCATTGGTGGAAGAGACAGGCATTCCTTTAGCGAAAAGGGTTTTGGAAAAAGCTCGGACACAAGGGGTTAAGATTTA
TCTTCCGGTAGATGCCAAAGTTGCAAGACGTTGCGAAACTGGGGAAGATTGGCAAGAGTTGTCAATACAGGAGGGGATTC
CTGAGGGTTTCTCTGGTTTCGATATTGGGTCTAAGACGATAGCTCTATTTTCTGAGGTGATCCAAGATTCCGCAACGGTA
TTTTGGAATGGCCCTGTTGGGGTTTATGAAGTTCCTCCTTTCGATCAAGGATCAAAAGCCATCGCTCAGTGTCTTGCAAG
TCATTCTTCTGCAGTGACGGTTGTTGGTGGGGGTGATGCCGCTGCCGTAGTTGCTTTAGCAGGATGTACCTCGCAGATTT
CCCATGTATCCACAGGAGGAGGAGCTTCTTTGGAATTCTTAGAGAAGGGCTATCTTCCAGGGACAGAAATTCTCTCCCCT
GCTCGCAGTTAA

Upstream 100 bases:

>100_bases
GTTTTTGTCTGAACAATTCTTCTTATTTAGCTTTGATAAAGGCTTGAGTTTTCTGTTGTTTAGGCGCTATAAGAAAATTT
AGGTTAAGGATCGGATAAGC

Downstream 100 bases:

>100_bases
ATATACTTTATTGATTCTCATCTGGTTAAAAAAAAAGCCCTGTTAGTCAGGGCTTTTTTTTGCTTAGTTATATCCGTTTA
TTAATAGCCTTATGTAAAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MDKLSIKDLSPEGKKVLVRVDFNVPIKDGKILDDVRIRSAMPTINYLLKRDAAIILVSHLGRPKGDMFEEAYSLAPIVPV
LEGYLGHHVPLSPDCVGEVARQAVAQLSPGRVLLLENVRFHRGEEHPEEDPSFAVELAAYADFYVNDAFGTSHRKHASVY
RVPQMFPDRAAAGFLMEKELEFLGQHLLVEPKRPFTAILGGAKVSSKIGVIEALLSRVDNLVLAGGMGYTFLKAMNQQVG
NSLVEETGIPLAKRVLEKARTQGVKIYLPVDAKVARRCETGEDWQELSIQEGIPEGFSGFDIGSKTIALFSEVIQDSATV
FWNGPVGVYEVPPFDQGSKAIAQCLASHSSAVTVVGGGDAAAVVALAGCTSQISHVSTGGGASLEFLEKGYLPGTEILSP
ARS

Sequences:

>Translated_403_residues
MDKLSIKDLSPEGKKVLVRVDFNVPIKDGKILDDVRIRSAMPTINYLLKRDAAIILVSHLGRPKGDMFEEAYSLAPIVPV
LEGYLGHHVPLSPDCVGEVARQAVAQLSPGRVLLLENVRFHRGEEHPEEDPSFAVELAAYADFYVNDAFGTSHRKHASVY
RVPQMFPDRAAAGFLMEKELEFLGQHLLVEPKRPFTAILGGAKVSSKIGVIEALLSRVDNLVLAGGMGYTFLKAMNQQVG
NSLVEETGIPLAKRVLEKARTQGVKIYLPVDAKVARRCETGEDWQELSIQEGIPEGFSGFDIGSKTIALFSEVIQDSATV
FWNGPVGVYEVPPFDQGSKAIAQCLASHSSAVTVVGGGDAAAVVALAGCTSQISHVSTGGGASLEFLEKGYLPGTEILSP
ARS
>Mature_403_residues
MDKLSIKDLSPEGKKVLVRVDFNVPIKDGKILDDVRIRSAMPTINYLLKRDAAIILVSHLGRPKGDMFEEAYSLAPIVPV
LEGYLGHHVPLSPDCVGEVARQAVAQLSPGRVLLLENVRFHRGEEHPEEDPSFAVELAAYADFYVNDAFGTSHRKHASVY
RVPQMFPDRAAAGFLMEKELEFLGQHLLVEPKRPFTAILGGAKVSSKIGVIEALLSRVDNLVLAGGMGYTFLKAMNQQVG
NSLVEETGIPLAKRVLEKARTQGVKIYLPVDAKVARRCETGEDWQELSIQEGIPEGFSGFDIGSKTIALFSEVIQDSATV
FWNGPVGVYEVPPFDQGSKAIAQCLASHSSAVTVVGGGDAAAVVALAGCTSQISHVSTGGGASLEFLEKGYLPGTEILSP
ARS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=42.0289855072464, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=41.4457831325301, Blast_Score=318, Evalue=4e-87,
Organism=Escherichia coli, GI1789294, Length=403, Percent_Identity=38.7096774193548, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17508823, Length=419, Percent_Identity=42.4821002386635, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6319857, Length=418, Percent_Identity=42.822966507177, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=42.822966507177, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=41.5676959619952, Blast_Score=305, Evalue=2e-83,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CHLMU (Q9PLN4)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_296449.1
- ProteinModelPortal:   Q9PLN4
- SMR:   Q9PLN4
- GeneID:   1245594
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0065
- TIGR:   TC_0065
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q9PLN4
- ProtClustDB:   PRK00073
- BioCyc:   CMUR243161:TC_0065-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43392; Mature: 43392

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 154-154 BINDING 207-207 BINDING 299-299 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLSIKDLSPEGKKVLVRVDFNVPIKDGKILDDVRIRSAMPTINYLLKRDAAIILVSHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GRPKGDMFEEAYSLAPIVPVLEGYLGHHVPLSPDCVGEVARQAVAQLSPGRVLLLENVRF
CCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHH
HRGEEHPEEDPSFAVELAAYADFYVNDAFGTSHRKHASVYRVPQMFPDRAAAGFLMEKEL
HCCCCCCCCCCCCEEHHHHHHHHHEECCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHH
EFLGQHLLVEPKRPFTAILGGAKVSSKIGVIEALLSRVDNLVLAGGMGYTFLKAMNQQVG
HHHHHHHEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
NSLVEETGIPLAKRVLEKARTQGVKIYLPVDAKVARRCETGEDWQELSIQEGIPEGFSGF
HHHHHHHCCHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCHHHHHHHHCCCCCCCCC
DIGSKTIALFSEVIQDSATVFWNGPVGVYEVPPFDQGSKAIAQCLASHSSAVTVVGGGDA
CCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCH
AAVVALAGCTSQISHVSTGGGASLEFLEKGYLPGTEILSPARS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCC
>Mature Secondary Structure
MDKLSIKDLSPEGKKVLVRVDFNVPIKDGKILDDVRIRSAMPTINYLLKRDAAIILVSHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GRPKGDMFEEAYSLAPIVPVLEGYLGHHVPLSPDCVGEVARQAVAQLSPGRVLLLENVRF
CCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHH
HRGEEHPEEDPSFAVELAAYADFYVNDAFGTSHRKHASVYRVPQMFPDRAAAGFLMEKEL
HCCCCCCCCCCCCEEHHHHHHHHHEECCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHH
EFLGQHLLVEPKRPFTAILGGAKVSSKIGVIEALLSRVDNLVLAGGMGYTFLKAMNQQVG
HHHHHHHEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
NSLVEETGIPLAKRVLEKARTQGVKIYLPVDAKVARRCETGEDWQELSIQEGIPEGFSGF
HHHHHHHCCHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCHHHHHHHHCCCCCCCCC
DIGSKTIALFSEVIQDSATVFWNGPVGVYEVPPFDQGSKAIAQCLASHSSAVTVVGGGDA
CCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCH
AAVVALAGCTSQISHVSTGGGASLEFLEKGYLPGTEILSPARS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935