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The map label for this gene is gyrB-1

Identifier: 15834657

GI number: 15834657

Start: 36406

End: 38223

Strand: Reverse

Name: gyrB-1

Synonym: TC0032

Alternate gene names: 15834657

Gene position: 38223-36406 (Counterclockwise)

Preceding gene: 15834671

Following gene: 15834656

Centisome position: 3.56

GC content: 38.78

Gene sequence:

>1818_bases
ATGCGAAAAAAAACTGCGTACTCAGAAGCCTCCATCATTTCTTTAGCTTCTCTAGATCATATTCGCCTCCGTGCAGGAAT
GTACATTGGAAGACTTGGAGATGGTTCTCAATCCGAAGATGGTGTGTACACTTTATTCAAGGAAGTGGTCGACAATGCTA
TTGATGAATTTGTCATGGGACATGGGCATACTATTTTTATAACTGGAGACTCACAAAAACTTTCCATTCGAGATGAAGGC
CGAGGGATCCCTTTAGGTAAGGTTATTGACTGCGTCTCCAAAATTAACACTGGGGCAAAGTATAACCAAGATGTTTTCCA
TTTTTCTGTTGGACTAAATGGTGTAGGGCTTAAAGCGGTTAACGCATTATCACAACACTTTTCTGTCCGTTCTGTGCGGA
ACAAAAAATTTCTCAAAGCATCTTTTTCTAAAGGCATCCTTTTGCATACCGAACAAGGAGACACTCAAGATGCTGATGGC
ACAGAAATTTCCTTTTCTCCTGATAAAGAATTATTCGAAAATTTCTCATTCCATGATGAATTTTTAAAAAAGAAAATACG
TCAATATACCTATCTCCATCCAGGTCTCACAATTCTTTATAATGGAGAAACCTTTGTCTCTAAGCTTGGGTTAGCAGATC
TTTTCGAAGAGGAAATTCAAACACCCCTTCTTTATCCTCCTATTGCTTTCCAACATCCTGATCTCTCCTTTCTTTTTTCC
CACACAGAAACCTCTTCAGAGCAATACTTTTCCTTTGTAAATGGCCAAGAAACCACTGATGGAGGAACCCATCTCATTGC
TTTTAAGGAAGGAGTTGTTAAGGGCATTAATGAATTTTTCAAGAAAAATTTCTCTGCTCAAGATATCCGTGACGGCATTG
CTGGGTGCATTGCCATTAAAATTGCTTCCCCCATTTTTGAATCACAGACCAAAAATAAACTTGGGAACACTAACATTCGT
GCAGATCTAGCTAAACGTGTGAAAGAAGCGGTGATCCTTACATTAAAAAAGCACCCGTCAAGCGCGGATCGCATTCAAGA
AAAAATCAAACTCAATGAGAAAACTCGGAAAAATGCTCAGTTTCTAAAACAAGAGCTAAAAGACAAACAGAAGAAGCTCC
ATTATAAAATCCCGAAACTCAGGGATTGCAAATTCCATCTCACTGACAACTCTTTATACGGCAACAATTCTTCTATTTTC
ATCACTGAAGGAGAATCTGCTTCTGCCTCTATTCTAGCTTCCCGTAATCCGCTCACACAAGCAGTCTTCTCTTTACGAGG
GAAACCCATGAATGTCTTTTCTTCAAAAGAAGAGACTATTTATAAAAATGATGAACTTTTTTACCTTGCCACAGCTCTGG
GGCTCCATAAGAATTCTCTACAGAATCTCCGCTATAATCACATTATCTTAGCTACAGATGCCGATGTTGATGGCATGCAC
ATTCGTAATTTGATGATTACTTTTTTCCTAAAAACTTTCCTGCCTGTAGTCGAACAAAATCATCTATTCATCTTAGAAAC
ACCGCTTTTTAAAGTCCGCTACAAGGATGAAACGTTTTATTGCTATTCAGAAGAAGAAAAACTCTCTACCATAGAACGGC
TGGGGAAAAAAGAATCTTCGCTTGAAATCACTCGATTTAAAGGTCTTGGTGAAATTTCTCCTAAAGAATTTAAATCTTTC
ATCGGACCCGACATGCGCTTAACTCCAGTCTCTCCCCCCAATGCAGATACTCTAGATGCACTTCTACAGTTCTACATGGG
GAAAAATACGAAAGAGCGGAAGCACTTTATTATTGAGAATCTAGTTACTAACCTATAG

Upstream 100 bases:

>100_bases
TATGTCTTACATTATTTTTCTAAAGAGAGCATAACTATTAGCTGCTTGTTTTCTTAGCAATTAGAACTCACATCAAACTC
TTATCAATTCTACGAACTTT

Downstream 100 bases:

>100_bases
CGACAAACACACTTATGAGCAATATCTCGGATCTATTTAAGACTCATTTCACACAATACGCGTCTTATGTCATCTTAGAG
CGCGCAATCCCCCATGTTTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRKKTAYSEASIISLASLDHIRLRAGMYIGRLGDGSQSEDGVYTLFKEVVDNAIDEFVMGHGHTIFITGDSQKLSIRDEG
RGIPLGKVIDCVSKINTGAKYNQDVFHFSVGLNGVGLKAVNALSQHFSVRSVRNKKFLKASFSKGILLHTEQGDTQDADG
TEISFSPDKELFENFSFHDEFLKKKIRQYTYLHPGLTILYNGETFVSKLGLADLFEEEIQTPLLYPPIAFQHPDLSFLFS
HTETSSEQYFSFVNGQETTDGGTHLIAFKEGVVKGINEFFKKNFSAQDIRDGIAGCIAIKIASPIFESQTKNKLGNTNIR
ADLAKRVKEAVILTLKKHPSSADRIQEKIKLNEKTRKNAQFLKQELKDKQKKLHYKIPKLRDCKFHLTDNSLYGNNSSIF
ITEGESASASILASRNPLTQAVFSLRGKPMNVFSSKEETIYKNDELFYLATALGLHKNSLQNLRYNHIILATDADVDGMH
IRNLMITFFLKTFLPVVEQNHLFILETPLFKVRYKDETFYCYSEEEKLSTIERLGKKESSLEITRFKGLGEISPKEFKSF
IGPDMRLTPVSPPNADTLDALLQFYMGKNTKERKHFIIENLVTNL

Sequences:

>Translated_605_residues
MRKKTAYSEASIISLASLDHIRLRAGMYIGRLGDGSQSEDGVYTLFKEVVDNAIDEFVMGHGHTIFITGDSQKLSIRDEG
RGIPLGKVIDCVSKINTGAKYNQDVFHFSVGLNGVGLKAVNALSQHFSVRSVRNKKFLKASFSKGILLHTEQGDTQDADG
TEISFSPDKELFENFSFHDEFLKKKIRQYTYLHPGLTILYNGETFVSKLGLADLFEEEIQTPLLYPPIAFQHPDLSFLFS
HTETSSEQYFSFVNGQETTDGGTHLIAFKEGVVKGINEFFKKNFSAQDIRDGIAGCIAIKIASPIFESQTKNKLGNTNIR
ADLAKRVKEAVILTLKKHPSSADRIQEKIKLNEKTRKNAQFLKQELKDKQKKLHYKIPKLRDCKFHLTDNSLYGNNSSIF
ITEGESASASILASRNPLTQAVFSLRGKPMNVFSSKEETIYKNDELFYLATALGLHKNSLQNLRYNHIILATDADVDGMH
IRNLMITFFLKTFLPVVEQNHLFILETPLFKVRYKDETFYCYSEEEKLSTIERLGKKESSLEITRFKGLGEISPKEFKSF
IGPDMRLTPVSPPNADTLDALLQFYMGKNTKERKHFIIENLVTNL
>Mature_605_residues
MRKKTAYSEASIISLASLDHIRLRAGMYIGRLGDGSQSEDGVYTLFKEVVDNAIDEFVMGHGHTIFITGDSQKLSIRDEG
RGIPLGKVIDCVSKINTGAKYNQDVFHFSVGLNGVGLKAVNALSQHFSVRSVRNKKFLKASFSKGILLHTEQGDTQDADG
TEISFSPDKELFENFSFHDEFLKKKIRQYTYLHPGLTILYNGETFVSKLGLADLFEEEIQTPLLYPPIAFQHPDLSFLFS
HTETSSEQYFSFVNGQETTDGGTHLIAFKEGVVKGINEFFKKNFSAQDIRDGIAGCIAIKIASPIFESQTKNKLGNTNIR
ADLAKRVKEAVILTLKKHPSSADRIQEKIKLNEKTRKNAQFLKQELKDKQKKLHYKIPKLRDCKFHLTDNSLYGNNSSIF
ITEGESASASILASRNPLTQAVFSLRGKPMNVFSSKEETIYKNDELFYLATALGLHKNSLQNLRYNHIILATDADVDGMH
IRNLMITFFLKTFLPVVEQNHLFILETPLFKVRYKDETFYCYSEEEKLSTIERLGKKESSLEITRFKGLGEISPKEFKSF
IGPDMRLTPVSPPNADTLDALLQFYMGKNTKERKHFIIENLVTNL

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=615, Percent_Identity=26.1788617886179, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI19913406, Length=633, Percent_Identity=25.9083728278041, Blast_Score=114, Evalue=4e-25,
Organism=Escherichia coli, GI1789408, Length=638, Percent_Identity=27.8996865203762, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI48994957, Length=569, Percent_Identity=31.1072056239016, Blast_Score=231, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17535065, Length=659, Percent_Identity=24.886191198786, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI212645845, Length=593, Percent_Identity=24.6205733558179, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=637, Percent_Identity=24.6467817896389, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17136538, Length=615, Percent_Identity=26.0162601626016, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3

Molecular weight: Translated: 68442; Mature: 68442

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKTAYSEASIISLASLDHIRLRAGMYIGRLGDGSQSEDGVYTLFKEVVDNAIDEFVMG
CCCCCCCCCHHEEEHHCCCHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
HGHTIFITGDSQKLSIRDEGRGIPLGKVIDCVSKINTGAKYNQDVFHFSVGLNGVGLKAV
CCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHH
NALSQHFSVRSVRNKKFLKASFSKGILLHTEQGDTQDADGTEISFSPDKELFENFSFHDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHCCCHHHH
FLKKKIRQYTYLHPGLTILYNGETFVSKLGLADLFEEEIQTPLLYPPIAFQHPDLSFLFS
HHHHHHHHHEEECCCEEEEECCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCHHEEEE
HTETSSEQYFSFVNGQETTDGGTHLIAFKEGVVKGINEFFKKNFSAQDIRDGIAGCIAIK
CCCCCHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEE
IASPIFESQTKNKLGNTNIRADLAKRVKEAVILTLKKHPSSADRIQEKIKLNEKTRKNAQ
EHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHH
FLKQELKDKQKKLHYKIPKLRDCKFHLTDNSLYGNNSSIFITEGESASASILASRNPLTQ
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCEEEEECCCCCCEEEEECCCHHHH
AVFSLRGKPMNVFSSKEETIYKNDELFYLATALGLHKNSLQNLRYNHIILATDADVDGMH
HHHHHCCCCCHHCCCCCCCEECCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
IRNLMITFFLKTFLPVVEQNHLFILETPLFKVRYKDETFYCYSEEEKLSTIERLGKKESS
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCC
LEITRFKGLGEISPKEFKSFIGPDMRLTPVSPPNADTLDALLQFYMGKNTKERKHFIIEN
EEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LVTNL
HHHCC
>Mature Secondary Structure
MRKKTAYSEASIISLASLDHIRLRAGMYIGRLGDGSQSEDGVYTLFKEVVDNAIDEFVMG
CCCCCCCCCHHEEEHHCCCHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
HGHTIFITGDSQKLSIRDEGRGIPLGKVIDCVSKINTGAKYNQDVFHFSVGLNGVGLKAV
CCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHH
NALSQHFSVRSVRNKKFLKASFSKGILLHTEQGDTQDADGTEISFSPDKELFENFSFHDE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHCCCHHHH
FLKKKIRQYTYLHPGLTILYNGETFVSKLGLADLFEEEIQTPLLYPPIAFQHPDLSFLFS
HHHHHHHHHEEECCCEEEEECCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCHHEEEE
HTETSSEQYFSFVNGQETTDGGTHLIAFKEGVVKGINEFFKKNFSAQDIRDGIAGCIAIK
CCCCCHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEE
IASPIFESQTKNKLGNTNIRADLAKRVKEAVILTLKKHPSSADRIQEKIKLNEKTRKNAQ
EHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHH
FLKQELKDKQKKLHYKIPKLRDCKFHLTDNSLYGNNSSIFITEGESASASILASRNPLTQ
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCEEEEECCCCCCEEEEECCCHHHH
AVFSLRGKPMNVFSSKEETIYKNDELFYLATALGLHKNSLQNLRYNHIILATDADVDGMH
HHHHHCCCCCHHCCCCCCCEECCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
IRNLMITFFLKTFLPVVEQNHLFILETPLFKVRYKDETFYCYSEEEKLSTIERLGKKESS
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCC
LEITRFKGLGEISPKEFKSFIGPDMRLTPVSPPNADTLDALLQFYMGKNTKERKHFIIEN
EEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LVTNL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]