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The map label for this gene is pnp [H]

Identifier: 158339001

GI number: 158339001

Start: 5983436

End: 5985376

Strand: Reverse

Name: pnp [H]

Synonym: AM1_5920

Alternate gene names: 158339001

Gene position: 5985376-5983436 (Counterclockwise)

Preceding gene: 158339002

Following gene: 158339000

Centisome position: 92.03

GC content: 49.87

Gene sequence:

>1941_bases
GTGCCGGGGGGCTTTCTACGACGGGAAGGTCGACCCCCTGAGAAAGCAACCTTAACCTGTCGCCTGATCGACCGACCCAT
GCGCCCCCTATTCCCGAATTGGTTGCGGGATGATTTGCAAATTGTGGCAACCACTATATCTATGGATGAGCAGGTTCCCC
CTGACGTCTTAGCCGTGACGGGGGCTTCTGTTGCCACCCTCTTGGCCAAAATACCTTTTAAAGGGCCCATGGCTGCCGTG
CGCGTTGGCCTAGTGGGGGATGACTTTATTATTAACCCCACCTATGAGGAGATCTCTGAAGGGGAACTAGATCTCGTTGT
TGCTGGTTCACCGGATGGGGTGATTATGGTTGAAGCTGGGGCCAATGAAGTGGCTGAGCAAGACATGATCGAGGCCATCG
ATTTTGGGTATGAGGTGGTTTGTGACCTCATTAAGGCTCAAAAAGAGTTGATAGCTGAGTTGGGGATTGAGCAGGTAGAA
GAAGCTGCTCCAGAAACGGATCCAACCCTAGAGAATTTTGTCCAAGAGCGCGTAACTGACCCTGTTAAAGACGTTCTAGC
CCGGTTTGAGAAGGATAAAAATGTTCGAGATGCTGCCTTAGATGAGATTAAGGAATCTGTGAAAGTTGCTATCGATGAGC
TTCCGGAAACAGATCCAGTACAGGTGGCAGCAGTAGAAAACCCCAAAGCTATTGGTAAGGTTTTTAAGAGTATTACTAAA
ACCTTAATGCGCCGCCAGGTGGTTGAAGATAAGGTCCGAGTGGATGGCCGTAAGTTAGATGAAGTTCGTCCTATTTCTTG
TAATGTGGGTGTTCTCCCCCAGCGGGTTCACGGCAGTGGCTTATTCAACCGTGGCTTAACCCAGGTTTTGTCTATCGCGA
CCTTGGGAACACCGGGTGATGCTCAAGAAATGGATGATCTCCATCCTGATAGCCAAAAGCGTTACCTTCATCACTATAAT
TTCCCGCCTTATTCTGTGGGTGAAACCCGACCAATGCGCTCGCCTGGACGTCGGGAAGTAGGGCATGGGGCTCTGGCTGA
AAGAGCTTTACTACCGATGCTTCCTAGTAAAGAAGAATTCCCCTACGTTCTTCGAGTCGTTTCCGAAGTCTTATCCTCTA
ACGGGTCTACATCAATGGGGTCAGTTTGCGGATCCACTTTGTCCTTGATGGATGCGGGAGTGCCCCTGGCTAAGCCAGTG
AGTGGAGCCGCTATGGGCTTAATCAAGGAAGGGGAAGAAGTTCGAGTACTCACAGATATTCAAGGGATTGAAGATTTCTT
GGGGGATATGGACTTTAAGGTAGCGGGTACTGCGGATGGCATTACCGCCCTGCAAATGGACATGAAAATCACCGGTTTAT
CGATGAAAGTGATCGCTGAGGCGATTCATCAAGCGAAACCCGCTCGACTCCATATTTTGGATAAGATGCTGGCGACAATT
GACAGTCCTCGCGAAACGATGTCTCCCTATGCACCTCGCCTATTGACCATCAAGATCGATCCAGAACAGATTGGCATGAT
TATTGGTCCAGGCGGTAAGACCATTAAAGGGATTACCGAGGAAACGGGGGCTAAGATTGATATCGAAGATGATGGTCGCG
TTACGATCTCAGCAGTAGATGAGAAGGCTGCATCTCGTGCTCGCCAGATTATTGCTGGTATGACACGCAAGCTGGCAGCT
GGTGATGTTTTCTTGGGTAAAGTGACTCGGATTATTCCGATTGGGGCCTTTGTGGAAATTGCGCCTAGTAAGGAGGGCAT
GATTCACATTTCTCAGTTGGCAGACTATCGAGTTGGCAAGGTGGAAGATGAAGTCACCGTTGGCGATGAAGTGGTGGTTA
AAATCCGTGAGCTAGACAATCGCGGTCGCATTAATCTGACTCGCTTGGGGATTCATCCTGAGGAAGCGGCGGCGGCTCGT
GCAGGTGGTTCTGAGTCTTAG

Upstream 100 bases:

>100_bases
TTCAGTTCTAGTCACTGCGACGAGATCGTCAGGACGTGAAGGAATTGATTTTCTTCCTTTACTCGTAGATTACGAGGAGA
GACTGTATGCAGCCGGTCGT

Downstream 100 bases:

>100_bases
TTAGCTTAGCTTTTTGATCATTGGCTTTGAGAGATGCTGTGGCGTTCCGCTGCAGCATTTCCAGTTTGATGCGGCGGAGG
GCAGAGGCTCTAAATCTGCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MPGGFLRREGRPPEKATLTCRLIDRPMRPLFPNWLRDDLQIVATTISMDEQVPPDVLAVTGASVATLLAKIPFKGPMAAV
RVGLVGDDFIINPTYEEISEGELDLVVAGSPDGVIMVEAGANEVAEQDMIEAIDFGYEVVCDLIKAQKELIAELGIEQVE
EAAPETDPTLENFVQERVTDPVKDVLARFEKDKNVRDAALDEIKESVKVAIDELPETDPVQVAAVENPKAIGKVFKSITK
TLMRRQVVEDKVRVDGRKLDEVRPISCNVGVLPQRVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPDSQKRYLHHYN
FPPYSVGETRPMRSPGRREVGHGALAERALLPMLPSKEEFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPLAKPV
SGAAMGLIKEGEEVRVLTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLSMKVIAEAIHQAKPARLHILDKMLATI
DSPRETMSPYAPRLLTIKIDPEQIGMIIGPGGKTIKGITEETGAKIDIEDDGRVTISAVDEKAASRARQIIAGMTRKLAA
GDVFLGKVTRIIPIGAFVEIAPSKEGMIHISQLADYRVGKVEDEVTVGDEVVVKIRELDNRGRINLTRLGIHPEEAAAAR
AGGSES

Sequences:

>Translated_646_residues
MPGGFLRREGRPPEKATLTCRLIDRPMRPLFPNWLRDDLQIVATTISMDEQVPPDVLAVTGASVATLLAKIPFKGPMAAV
RVGLVGDDFIINPTYEEISEGELDLVVAGSPDGVIMVEAGANEVAEQDMIEAIDFGYEVVCDLIKAQKELIAELGIEQVE
EAAPETDPTLENFVQERVTDPVKDVLARFEKDKNVRDAALDEIKESVKVAIDELPETDPVQVAAVENPKAIGKVFKSITK
TLMRRQVVEDKVRVDGRKLDEVRPISCNVGVLPQRVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPDSQKRYLHHYN
FPPYSVGETRPMRSPGRREVGHGALAERALLPMLPSKEEFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPLAKPV
SGAAMGLIKEGEEVRVLTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLSMKVIAEAIHQAKPARLHILDKMLATI
DSPRETMSPYAPRLLTIKIDPEQIGMIIGPGGKTIKGITEETGAKIDIEDDGRVTISAVDEKAASRARQIIAGMTRKLAA
GDVFLGKVTRIIPIGAFVEIAPSKEGMIHISQLADYRVGKVEDEVTVGDEVVVKIRELDNRGRINLTRLGIHPEEAAAAR
AGGSES
>Mature_645_residues
PGGFLRREGRPPEKATLTCRLIDRPMRPLFPNWLRDDLQIVATTISMDEQVPPDVLAVTGASVATLLAKIPFKGPMAAVR
VGLVGDDFIINPTYEEISEGELDLVVAGSPDGVIMVEAGANEVAEQDMIEAIDFGYEVVCDLIKAQKELIAELGIEQVEE
AAPETDPTLENFVQERVTDPVKDVLARFEKDKNVRDAALDEIKESVKVAIDELPETDPVQVAAVENPKAIGKVFKSITKT
LMRRQVVEDKVRVDGRKLDEVRPISCNVGVLPQRVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPDSQKRYLHHYNF
PPYSVGETRPMRSPGRREVGHGALAERALLPMLPSKEEFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPLAKPVS
GAAMGLIKEGEEVRVLTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLSMKVIAEAIHQAKPARLHILDKMLATID
SPRETMSPYAPRLLTIKIDPEQIGMIIGPGGKTIKGITEETGAKIDIEDDGRVTISAVDEKAASRARQIIAGMTRKLAAG
DVFLGKVTRIIPIGAFVEIAPSKEGMIHISQLADYRVGKVEDEVTVGDEVVVKIRELDNRGRINLTRLGIHPEEAAAARA
GGSES

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:
GO:0000175: Polyribonucleotide nucleotidyltransferase
GO:0003723: Polyribonucleotide nucleotidyltransferase
GO:0004654: Polyribonucleotide nucleotidyltransferase
GO:0005737: Polyribonucleotide nucleotidyltransferase
GO:0006396: Polyribonucleotide nucleotidyltransferase
GO:0006402: Polyribonucleotide nucleotidyltransferase
GO:0016740: Polyribonucleotide nucleotidyltransferase
GO:0016779: Polyribonucleotide nucleotidyltransferase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=651, Percent_Identity=36.7127496159754, Blast_Score=398, Evalue=1e-110,
Organism=Escherichia coli, GI145693187, Length=632, Percent_Identity=47.7848101265823, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI115534063, Length=600, Percent_Identity=34.5, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI281362905, Length=636, Percent_Identity=38.6792452830189, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=636, Percent_Identity=38.6792452830189, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=636, Percent_Identity=38.6792452830189, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=636, Percent_Identity=38.6792452830189, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 69927; Mature: 69796

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGGFLRREGRPPEKATLTCRLIDRPMRPLFPNWLRDDLQIVATTISMDEQVPPDVLAVT
CCCCCCCCCCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GASVATLLAKIPFKGPMAAVRVGLVGDDFIINPTYEEISEGELDLVVAGSPDGVIMVEAG
CHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCHHHHCCCCEEEEEECCCCCEEEEECC
ANEVAEQDMIEAIDFGYEVVCDLIKAQKELIAELGIEQVEEAAPETDPTLENFVQERVTD
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCC
PVKDVLARFEKDKNVRDAALDEIKESVKVAIDELPETDPVQVAAVENPKAIGKVFKSITK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHH
TLMRRQVVEDKVRVDGRKLDEVRPISCNVGVLPQRVHGSGLFNRGLTQVLSIATLGTPGD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AQEMDDLHPDSQKRYLHHYNFPPYSVGETRPMRSPGRREVGHGALAERALLPMLPSKEEF
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCC
PYVLRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPLAKPVSGAAMGLIKEGEEVRVLTDI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEHHH
QGIEDFLGDMDFKVAGTADGITALQMDMKITGLSMKVIAEAIHQAKPARLHILDKMLATI
HHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHH
DSPRETMSPYAPRLLTIKIDPEQIGMIIGPGGKTIKGITEETGAKIDIEDDGRVTISAVD
CCCHHHCCCCCCEEEEEEECHHHCCEEECCCCCCCCCCHHCCCCEEEECCCCCEEEEEHH
EKAASRARQIIAGMTRKLAAGDVFLGKVTRIIPIGAFVEIAPSKEGMIHISQLADYRVGK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCEEEECCCCCCCEEHHHHHCCCCCC
VEDEVTVGDEVVVKIRELDNRGRINLTRLGIHPEEAAAARAGGSES
CCCCEECCHHHEEEEECCCCCCCEEEEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
PGGFLRREGRPPEKATLTCRLIDRPMRPLFPNWLRDDLQIVATTISMDEQVPPDVLAVT
CCCCCCCCCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GASVATLLAKIPFKGPMAAVRVGLVGDDFIINPTYEEISEGELDLVVAGSPDGVIMVEAG
CHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCHHHHCCCCEEEEEECCCCCEEEEECC
ANEVAEQDMIEAIDFGYEVVCDLIKAQKELIAELGIEQVEEAAPETDPTLENFVQERVTD
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCC
PVKDVLARFEKDKNVRDAALDEIKESVKVAIDELPETDPVQVAAVENPKAIGKVFKSITK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHH
TLMRRQVVEDKVRVDGRKLDEVRPISCNVGVLPQRVHGSGLFNRGLTQVLSIATLGTPGD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AQEMDDLHPDSQKRYLHHYNFPPYSVGETRPMRSPGRREVGHGALAERALLPMLPSKEEF
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCC
PYVLRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPLAKPVSGAAMGLIKEGEEVRVLTDI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEHHH
QGIEDFLGDMDFKVAGTADGITALQMDMKITGLSMKVIAEAIHQAKPARLHILDKMLATI
HHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHH
DSPRETMSPYAPRLLTIKIDPEQIGMIIGPGGKTIKGITEETGAKIDIEDDGRVTISAVD
CCCHHHCCCCCCEEEEEEECHHHCCEEECCCCCCCCCCHHCCCCEEEECCCCCEEEEEHH
EKAASRARQIIAGMTRKLAAGDVFLGKVTRIIPIGAFVEIAPSKEGMIHISQLADYRVGK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCEEEECCCCCCCEEHHHHHCCCCCC
VEDEVTVGDEVVVKIRELDNRGRINLTRLGIHPEEAAAARAGGSES
CCCCEECCHHHEEEEECCCCCCCEEEEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA