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The map label for this gene is hisF [H]

Identifier: 158338877

GI number: 158338877

Start: 5873852

End: 5874619

Strand: Direct

Name: hisF [H]

Synonym: AM1_5790

Alternate gene names: 158338877

Gene position: 5873852-5874619 (Clockwise)

Preceding gene: 158338876

Following gene: 158338878

Centisome position: 90.32

GC content: 51.17

Gene sequence:

>768_bases
ATGCTAGCCAAGCGAATTTTGCCTTGTTTAGACGTTAAAGCTGGACGAGTCGTCAAAGGGGTGAATTTCGTCAACCTAAA
AGATGCTGGAGATCCGGTTGAACTCGCCCAAATATATAACGAGGCTGGAGCCGATGAGTTAGTGTTCCTAGACATCACCG
CCACCCATGAAGATCGCAACATTATCTTTGATGTGGTGTACCGGACTGCCGAAGCCGTCTTTATTCCCCTGACCGTAGGC
GGTGGGATCCAATCCTTAGAAACGATTAAAAAGCTGTTGCGAGCTGGAGCCGATAAAGTCAGCTTAAACTCTTTCGCCGT
CCGTAATCCCGACTTTGTAAACCAAGCCAGCGAACGATTTGGTCGGCAATGCATTGTCGTGGCCATAGATGCCCGTCGTC
GTGATGATGGCAATTCCGGCTGGGATGTCTTTGTGCGTGGCGGTCGCGATAACACAGGTTTAGATGCGATCTCATGGGCC
AAGGAAATGGAAGAACGCGGTGCAGGAGAACTCTTAGTTACCAGCATGGATGGGGATGGTACCCAAGCTGGATATGACTT
GGAACTGACTCGCACCATTGCCGAACAAGTCGAGATCCCCGTGATCGCGTCTGGTGGCGCGGGCACCTGTGATCATATTC
GGGAAGCCTTGACAGACGGTAAGGCTGAAGCGGCTTTGCTAGCATCCCTACTGCACTACGGGCAGCTCTCTATTGCAGAA
ATAAAAACTCATTTGCAGGATCACCGAGTTCCGATCCGCCTGGCTTAA

Upstream 100 bases:

>100_bases
ATCATCCGAATCAAAAAATAGGACTGAGTAGCTTAGGATAATATTTGGCCAGGATGTTCACCGAACATTCCTGTCCTATC
CCATTGCAAGAGTATGAGCC

Downstream 100 bases:

>100_bases
TTCTTAAATCAAACTGTAAAATCACAGATTTGTGGTGGATTAGTCTTTAATTTTATTAAAATTAGTAAAGTTATGATTAT
TCTGCTTGTCGGTGCTATTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLAKRILPCLDVKAGRVVKGVNFVNLKDAGDPVELAQIYNEAGADELVFLDITATHEDRNIIFDVVYRTAEAVFIPLTVG
GGIQSLETIKKLLRAGADKVSLNSFAVRNPDFVNQASERFGRQCIVVAIDARRRDDGNSGWDVFVRGGRDNTGLDAISWA
KEMEERGAGELLVTSMDGDGTQAGYDLELTRTIAEQVEIPVIASGGAGTCDHIREALTDGKAEAALLASLLHYGQLSIAE
IKTHLQDHRVPIRLA

Sequences:

>Translated_255_residues
MLAKRILPCLDVKAGRVVKGVNFVNLKDAGDPVELAQIYNEAGADELVFLDITATHEDRNIIFDVVYRTAEAVFIPLTVG
GGIQSLETIKKLLRAGADKVSLNSFAVRNPDFVNQASERFGRQCIVVAIDARRRDDGNSGWDVFVRGGRDNTGLDAISWA
KEMEERGAGELLVTSMDGDGTQAGYDLELTRTIAEQVEIPVIASGGAGTCDHIREALTDGKAEAALLASLLHYGQLSIAE
IKTHLQDHRVPIRLA
>Mature_255_residues
MLAKRILPCLDVKAGRVVKGVNFVNLKDAGDPVELAQIYNEAGADELVFLDITATHEDRNIIFDVVYRTAEAVFIPLTVG
GGIQSLETIKKLLRAGADKVSLNSFAVRNPDFVNQASERFGRQCIVVAIDARRRDDGNSGWDVFVRGGRDNTGLDAISWA
KEMEERGAGELLVTSMDGDGTQAGYDLELTRTIAEQVEIPVIASGGAGTCDHIREALTDGKAEAALLASLLHYGQLSIAE
IKTHLQDHRVPIRLA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=45.3846153846154, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=29.7071129707113, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=31.2101910828025, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27612; Mature: 27612

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRILPCLDVKAGRVVKGVNFVNLKDAGDPVELAQIYNEAGADELVFLDITATHEDRN
CCHHHHCHHCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
IIFDVVYRTAEAVFIPLTVGGGIQSLETIKKLLRAGADKVSLNSFAVRNPDFVNQASERF
HHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHH
GRQCIVVAIDARRRDDGNSGWDVFVRGGRDNTGLDAISWAKEMEERGAGELLVTSMDGDG
CCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TQAGYDLELTRTIAEQVEIPVIASGGAGTCDHIREALTDGKAEAALLASLLHYGQLSIAE
CCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
IKTHLQDHRVPIRLA
HHHHHHHCCCCEEEC
>Mature Secondary Structure
MLAKRILPCLDVKAGRVVKGVNFVNLKDAGDPVELAQIYNEAGADELVFLDITATHEDRN
CCHHHHCHHCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
IIFDVVYRTAEAVFIPLTVGGGIQSLETIKKLLRAGADKVSLNSFAVRNPDFVNQASERF
HHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHH
GRQCIVVAIDARRRDDGNSGWDVFVRGGRDNTGLDAISWAKEMEERGAGELLVTSMDGDG
CCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
TQAGYDLELTRTIAEQVEIPVIASGGAGTCDHIREALTDGKAEAALLASLLHYGQLSIAE
CCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
IKTHLQDHRVPIRLA
HHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]