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The map label for this gene is ytcB [H]

Identifier: 158338858

GI number: 158338858

Start: 5852428

End: 5853303

Strand: Direct

Name: ytcB [H]

Synonym: AM1_5771

Alternate gene names: 158338858

Gene position: 5852428-5853303 (Clockwise)

Preceding gene: 158338857

Following gene: 158338859

Centisome position: 89.99

GC content: 36.3

Gene sequence:

>876_bases
ATGCAAGGTAACACTAGAGTATTGATTACAGGGGCTAACGGATTACTTGGAAGCCATACTATTGATGCAATACGCCACCA
ATGCCATGTAGAAGCAGTTGTCAGGCAGCTACCTAAACTCATATCAAGAGATGTTTCCTATCACTTAATCGATTTTAGCA
AGAACTGGAAAGTAGCTGATCTACCTAAGTCTGTAGACATTATTATTCATCTGGCCCAATCCGAACATTTCAGGGAGTTT
CCGGAACAAGCTATGGATGTTTTTCAGGTAAATATTGAATCTACAGCTCGATTGTTGGATTATGCATTTAAGAGTGGTGC
TAAAAAGTTTATTTATGCTTCTTCTGGAGGAGTTTATGGAAATGGCCCAATAGCTTTTCATGAAAATTCGTCGATTGTTA
GCCATCAAAAGCTAGGTCATTATCTGGGTAGCAAAGTTTGTGGAGAGGTTCTTGTACAGAGTTATGCATCAATATTTGAT
GTAATTATTCTTCGTCCATTTTTTATGTACGGTGCTAATCAACGCCGTACTATGTTGATTCCACGATTAGTCGATTGTGT
TGTTGAAGGGCGGCCAATTATTCTTCAGGGAAAGGATGGGATTCGAATCAATCCCGTACATGTACGAGATGTTGTCGATG
TTATAAAGTTTTGCTTAACTAGTAGTGGTAGTCTAACTTTAAATGTCGCGGGACCAAGTATTCTCAGCATTAAAGAAATT
TCTGAGATAATTGCAAATATTGTAAATAAGAAACCTCACTACTATATAACTAATGAGATCAAAAATGATCTTATTGGTGA
CAATGAACTAATGCTATCAATATTAGATAAAGAACTAATTCTCTTGAATGAGGGCATACTAGAATTAATTTATTAA

Upstream 100 bases:

>100_bases
GCCGATTCCATTCAATGGTCGGTTGTTATCTATAAGTTGTTATTTATACTTGTATTTTTGGGAATAGCTTATATAACACT
TCCTCTAAAGAGTTTAAATG

Downstream 100 bases:

>100_bases
ATTTATATTATTCTCAATATTGGTTATTGTATGCGAGTTCTTCATGCACCCACGACAGTGGGAGGTAATCCTCAAGGATT
AAGTAAGGCATTAAATAAAC

Product: NAD dependent epimerase/dehydratase family protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQGNTRVLITGANGLLGSHTIDAIRHQCHVEAVVRQLPKLISRDVSYHLIDFSKNWKVADLPKSVDIIIHLAQSEHFREF
PEQAMDVFQVNIESTARLLDYAFKSGAKKFIYASSGGVYGNGPIAFHENSSIVSHQKLGHYLGSKVCGEVLVQSYASIFD
VIILRPFFMYGANQRRTMLIPRLVDCVVEGRPIILQGKDGIRINPVHVRDVVDVIKFCLTSSGSLTLNVAGPSILSIKEI
SEIIANIVNKKPHYYITNEIKNDLIGDNELMLSILDKELILLNEGILELIY

Sequences:

>Translated_291_residues
MQGNTRVLITGANGLLGSHTIDAIRHQCHVEAVVRQLPKLISRDVSYHLIDFSKNWKVADLPKSVDIIIHLAQSEHFREF
PEQAMDVFQVNIESTARLLDYAFKSGAKKFIYASSGGVYGNGPIAFHENSSIVSHQKLGHYLGSKVCGEVLVQSYASIFD
VIILRPFFMYGANQRRTMLIPRLVDCVVEGRPIILQGKDGIRINPVHVRDVVDVIKFCLTSSGSLTLNVAGPSILSIKEI
SEIIANIVNKKPHYYITNEIKNDLIGDNELMLSILDKELILLNEGILELIY
>Mature_291_residues
MQGNTRVLITGANGLLGSHTIDAIRHQCHVEAVVRQLPKLISRDVSYHLIDFSKNWKVADLPKSVDIIIHLAQSEHFREF
PEQAMDVFQVNIESTARLLDYAFKSGAKKFIYASSGGVYGNGPIAFHENSSIVSHQKLGHYLGSKVCGEVLVQSYASIFD
VIILRPFFMYGANQRRTMLIPRLVDCVVEGRPIILQGKDGIRINPVHVRDVVDVIKFCLTSSGSLTLNVAGPSILSIKEI
SEIIANIVNKKPHYYITNEIKNDLIGDNELMLSILDKELILLNEGILELIY

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=227, Percent_Identity=28.1938325991189, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=247, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 32480; Mature: 32480

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGNTRVLITGANGLLGSHTIDAIRHQCHVEAVVRQLPKLISRDVSYHLIDFSKNWKVAD
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEC
LPKSVDIIIHLAQSEHFREFPEQAMDVFQVNIESTARLLDYAFKSGAKKFIYASSGGVYG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
NGPIAFHENSSIVSHQKLGHYLGSKVCGEVLVQSYASIFDVIILRPFFMYGANQRRTMLI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
PRLVDCVVEGRPIILQGKDGIRINPVHVRDVVDVIKFCLTSSGSLTLNVAGPSILSIKEI
HHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
SEIIANIVNKKPHYYITNEIKNDLIGDNELMLSILDKELILLNEGILELIY
HHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGNTRVLITGANGLLGSHTIDAIRHQCHVEAVVRQLPKLISRDVSYHLIDFSKNWKVAD
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEC
LPKSVDIIIHLAQSEHFREFPEQAMDVFQVNIESTARLLDYAFKSGAKKFIYASSGGVYG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
NGPIAFHENSSIVSHQKLGHYLGSKVCGEVLVQSYASIFDVIILRPFFMYGANQRRTMLI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
PRLVDCVVEGRPIILQGKDGIRINPVHVRDVVDVIKFCLTSSGSLTLNVAGPSILSIKEI
HHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
SEIIANIVNKKPHYYITNEIKNDLIGDNELMLSILDKELILLNEGILELIY
HHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]