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The map label for this gene is asnB [H]

Identifier: 158338851

GI number: 158338851

Start: 5844675

End: 5846558

Strand: Direct

Name: asnB [H]

Synonym: AM1_5764

Alternate gene names: 158338851

Gene position: 5844675-5846558 (Clockwise)

Preceding gene: 158338850

Following gene: 158338852

Centisome position: 89.87

GC content: 42.3

Gene sequence:

>1884_bases
ATGTGCGGTATAGCAGGGCTGATCCATATTAATGGTGATCCCGTCTCTCCGGTGATTTTGCAGCACATGACTGATGCGAT
TGCCCATCGTGGCCCTGATGGTGAAGGGCATTGGATTGAAGCTAATGTGGGTTTGGGCCATCGCCGTTTAGCTATCTTGG
ATTTGTCTCCTGCAGGACATCAGCCGATGGTATCGGCAGATCACCGTTTTGTGCTGAGCTATAACGGAGAGATTTATAAC
TATCAGACACTTCGCGCTGAATTGGAGACGATTGGCTACTGGTTTCGTTCTAAAACAGATAGTGAAGTGGTTCTCTATGC
ATTGGCAGAATGGGGAGTAAAGGCATTTGATCGGTTAAACGGAATGTTTGCCTTGGCTCTATGGGACCGTAAAACGAAAT
CGCTTTTACTCGCCCGAGATCGCTACGGTATTAAACCTTTATATTACACACAGCAAGGTAGTCTGTTTACGTTTGGATCT
GAGCAAAAAGCTTTGCTAGCGATACCCGGATTTGAACGTCGTCTAGATAAACCAGCACTACTCGAATATTTTACGTTTCA
GAATTTTTTTACGGATCGCACCTTGCTTGAAGGGGTCAGATTACTACCTGCAGGTCATTATGCCATTTTGGATCTGGGGC
AAAGTTCACCCGGCCTGAATATAAAGCAGTACTGGGATTATAACTTTTATGAACCAGCAGAGCCGATCAATGAGCAAGAT
TATAGAGAAGAATTAGATCGACTATTTCAACAAGCTGTCAATCGGCAATTGGTAAGCGATGTTGAGTTAGGATGCTACCT
CAGTGGTGGGATAGATTCAGGTTCAATTAGTGCGATCGCAGCCCGTTCCTATCCTTATCTAAAAAGTTTTACCTGTGGTT
TTGATCTAAGTTCAGCTTCTGGGATTGAGCTTGCCTTTGACGAGCGGGCTCAAGCGGAAGCGATGTCTTACCACTTCAAA
ACAGAGCATTATGAAATGGTGCTCAAAGCAGGGGATATGGAACGGGTTCTACCAAAGCTAGCATGGCATTTAGAAGAACC
TCGGGTAGGACAAAGTTACCCCAACTATTATGCCGCTCAGCTTGCAAGTAAGTTTGTAAAAGTAGTTTTAAGTGGTGCAG
GAGGAGATGAGCTTTTTGGAGGTTATCCCTGGCGCTATTACCGAGCAGTAGTCAATGAAGATTTTGAAAACTATATCGAT
AAGTACTACTTGTTTTGGCATCGCCTAATTCCTAACAGCCAACTTAAGAAAGTGTTTGCGCCCATATGGAATGATGTAAG
CCAAGTATGGACACGTGACATTTTTCGTGATGTGTTTCAACATCACCCAGACCGTTTAGAAGGACCAAAGGATTACATTA
ACCACTCGCTATATTTTGAAGCGAAAACGTTTCTACATGGCCTATTAGTTGTGGAAGACAAGCTAAGCATGGCTCATGGC
TTAGAAAGCCGTGTGCCTTTTTTAGATAATGATTTAGTCAATTTTGCTATGGGGTGTCCAGTCAACTTAAAACTGAATAA
TTTGGCTGAGGTGGTGCGACTTAATGAAAATGATGTTGGCAACAAACCCCAAAAATTTTTTCAGAAAACTCGAGATGGTA
AACAGATATTCCGGGATGTGATGCAGAAATATATCTCTGAAGACATTACTAAGGCTGAGAAGCAAGGATTTTCTGCTCCA
GATGCAAGTTGGTTCAAAGGTGACAGTATTGAGTTTGTGAAACGGAAATTATTAGACCCAAATGCTGCAATTTATTCACT
AGTAGATAGAAAGGCAGTGCAGGATTTGATTGAAGAACACTTAAATGGACAAAAAAATCGCCGTCTACTAATTTGGTCTT
TGCTGAATGTAGAAGAATTCATTCAGCAGATAGGTAGTTTGTAA

Upstream 100 bases:

>100_bases
AGATTGCGATCGCTTCACGCTAGCATTGCCCCTTCATAATCAAATGACTGCGTCCGACTATAGATATGTGGTGGCAGCCC
TCAAGGAGCTGGAGTAATCT

Downstream 100 bases:

>100_bases
TGGATATCCATCTAGAAATTCATAATAGCGATAATGTCAATACCGAAGAATTATTAACGCTTAGTTGCAATTCTTTGATG
CACTATACACCTCAATACTT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MCGIAGLIHINGDPVSPVILQHMTDAIAHRGPDGEGHWIEANVGLGHRRLAILDLSPAGHQPMVSADHRFVLSYNGEIYN
YQTLRAELETIGYWFRSKTDSEVVLYALAEWGVKAFDRLNGMFALALWDRKTKSLLLARDRYGIKPLYYTQQGSLFTFGS
EQKALLAIPGFERRLDKPALLEYFTFQNFFTDRTLLEGVRLLPAGHYAILDLGQSSPGLNIKQYWDYNFYEPAEPINEQD
YREELDRLFQQAVNRQLVSDVELGCYLSGGIDSGSISAIAARSYPYLKSFTCGFDLSSASGIELAFDERAQAEAMSYHFK
TEHYEMVLKAGDMERVLPKLAWHLEEPRVGQSYPNYYAAQLASKFVKVVLSGAGGDELFGGYPWRYYRAVVNEDFENYID
KYYLFWHRLIPNSQLKKVFAPIWNDVSQVWTRDIFRDVFQHHPDRLEGPKDYINHSLYFEAKTFLHGLLVVEDKLSMAHG
LESRVPFLDNDLVNFAMGCPVNLKLNNLAEVVRLNENDVGNKPQKFFQKTRDGKQIFRDVMQKYISEDITKAEKQGFSAP
DASWFKGDSIEFVKRKLLDPNAAIYSLVDRKAVQDLIEEHLNGQKNRRLLIWSLLNVEEFIQQIGSL

Sequences:

>Translated_627_residues
MCGIAGLIHINGDPVSPVILQHMTDAIAHRGPDGEGHWIEANVGLGHRRLAILDLSPAGHQPMVSADHRFVLSYNGEIYN
YQTLRAELETIGYWFRSKTDSEVVLYALAEWGVKAFDRLNGMFALALWDRKTKSLLLARDRYGIKPLYYTQQGSLFTFGS
EQKALLAIPGFERRLDKPALLEYFTFQNFFTDRTLLEGVRLLPAGHYAILDLGQSSPGLNIKQYWDYNFYEPAEPINEQD
YREELDRLFQQAVNRQLVSDVELGCYLSGGIDSGSISAIAARSYPYLKSFTCGFDLSSASGIELAFDERAQAEAMSYHFK
TEHYEMVLKAGDMERVLPKLAWHLEEPRVGQSYPNYYAAQLASKFVKVVLSGAGGDELFGGYPWRYYRAVVNEDFENYID
KYYLFWHRLIPNSQLKKVFAPIWNDVSQVWTRDIFRDVFQHHPDRLEGPKDYINHSLYFEAKTFLHGLLVVEDKLSMAHG
LESRVPFLDNDLVNFAMGCPVNLKLNNLAEVVRLNENDVGNKPQKFFQKTRDGKQIFRDVMQKYISEDITKAEKQGFSAP
DASWFKGDSIEFVKRKLLDPNAAIYSLVDRKAVQDLIEEHLNGQKNRRLLIWSLLNVEEFIQQIGSL
>Mature_627_residues
MCGIAGLIHINGDPVSPVILQHMTDAIAHRGPDGEGHWIEANVGLGHRRLAILDLSPAGHQPMVSADHRFVLSYNGEIYN
YQTLRAELETIGYWFRSKTDSEVVLYALAEWGVKAFDRLNGMFALALWDRKTKSLLLARDRYGIKPLYYTQQGSLFTFGS
EQKALLAIPGFERRLDKPALLEYFTFQNFFTDRTLLEGVRLLPAGHYAILDLGQSSPGLNIKQYWDYNFYEPAEPINEQD
YREELDRLFQQAVNRQLVSDVELGCYLSGGIDSGSISAIAARSYPYLKSFTCGFDLSSASGIELAFDERAQAEAMSYHFK
TEHYEMVLKAGDMERVLPKLAWHLEEPRVGQSYPNYYAAQLASKFVKVVLSGAGGDELFGGYPWRYYRAVVNEDFENYID
KYYLFWHRLIPNSQLKKVFAPIWNDVSQVWTRDIFRDVFQHHPDRLEGPKDYINHSLYFEAKTFLHGLLVVEDKLSMAHG
LESRVPFLDNDLVNFAMGCPVNLKLNNLAEVVRLNENDVGNKPQKFFQKTRDGKQIFRDVMQKYISEDITKAEKQGFSAP
DASWFKGDSIEFVKRKLLDPNAAIYSLVDRKAVQDLIEEHLNGQKNRRLLIWSLLNVEEFIQQIGSL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=397, Percent_Identity=29.9748110831234, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI168229252, Length=397, Percent_Identity=29.9748110831234, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI168229250, Length=397, Percent_Identity=29.9748110831234, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI296010848, Length=371, Percent_Identity=29.6495956873315, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI296010852, Length=301, Percent_Identity=27.906976744186, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI296010850, Length=301, Percent_Identity=27.906976744186, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786889, Length=403, Percent_Identity=32.5062034739454, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=484, Percent_Identity=27.8925619834711, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=484, Percent_Identity=27.8925619834711, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=410, Percent_Identity=29.2682926829268, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=418, Percent_Identity=29.1866028708134, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=29.1169451073986, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45553209, Length=518, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71847; Mature: 71847

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGLIHINGDPVSPVILQHMTDAIAHRGPDGEGHWIEANVGLGHRRLAILDLSPAGH
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCC
QPMVSADHRFVLSYNGEIYNYQTLRAELETIGYWFRSKTDSEVVLYALAEWGVKAFDRLN
CCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHC
GMFALALWDRKTKSLLLARDRYGIKPLYYTQQGSLFTFGSEQKALLAIPGFERRLDKPAL
CEEEEEEECCCHHHHEEECCCCCCCCEEEECCCCEEEECCCCCEEEECCCHHHHCCCHHH
LEYFTFQNFFTDRTLLEGVRLLPAGHYAILDLGQSSPGLNIKQYWDYNFYEPAEPINEQD
HHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHH
YREELDRLFQQAVNRQLVSDVELGCYLSGGIDSGSISAIAARSYPYLKSFTCGFDLSSAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHEECCCCCCCCC
GIELAFDERAQAEAMSYHFKTEHYEMVLKAGDMERVLPKLAWHLEEPRVGQSYPNYYAAQ
CCEEEECCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LASKFVKVVLSGAGGDELFGGYPWRYYRAVVNEDFENYIDKYYLFWHRLIPNSQLKKVFA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PIWNDVSQVWTRDIFRDVFQHHPDRLEGPKDYINHSLYFEAKTFLHGLLVVEDKLSMAHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHC
LESRVPFLDNDLVNFAMGCPVNLKLNNLAEVVRLNENDVGNKPQKFFQKTRDGKQIFRDV
HHHCCCCCCCCHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHH
MQKYISEDITKAEKQGFSAPDASWFKGDSIEFVKRKLLDPNAAIYSLVDRKAVQDLIEEH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LNGQKNRRLLIWSLLNVEEFIQQIGSL
HCCCCCCEEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGLIHINGDPVSPVILQHMTDAIAHRGPDGEGHWIEANVGLGHRRLAILDLSPAGH
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCC
QPMVSADHRFVLSYNGEIYNYQTLRAELETIGYWFRSKTDSEVVLYALAEWGVKAFDRLN
CCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHC
GMFALALWDRKTKSLLLARDRYGIKPLYYTQQGSLFTFGSEQKALLAIPGFERRLDKPAL
CEEEEEEECCCHHHHEEECCCCCCCCEEEECCCCEEEECCCCCEEEECCCHHHHCCCHHH
LEYFTFQNFFTDRTLLEGVRLLPAGHYAILDLGQSSPGLNIKQYWDYNFYEPAEPINEQD
HHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHH
YREELDRLFQQAVNRQLVSDVELGCYLSGGIDSGSISAIAARSYPYLKSFTCGFDLSSAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHEECCCCCCCCC
GIELAFDERAQAEAMSYHFKTEHYEMVLKAGDMERVLPKLAWHLEEPRVGQSYPNYYAAQ
CCEEEECCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LASKFVKVVLSGAGGDELFGGYPWRYYRAVVNEDFENYIDKYYLFWHRLIPNSQLKKVFA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PIWNDVSQVWTRDIFRDVFQHHPDRLEGPKDYINHSLYFEAKTFLHGLLVVEDKLSMAHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHC
LESRVPFLDNDLVNFAMGCPVNLKLNNLAEVVRLNENDVGNKPQKFFQKTRDGKQIFRDV
HHHCCCCCCCCHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHH
MQKYISEDITKAEKQGFSAPDASWFKGDSIEFVKRKLLDPNAAIYSLVDRKAVQDLIEEH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LNGQKNRRLLIWSLLNVEEFIQQIGSL
HCCCCCCEEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]