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The map label for this gene is gmd [H]

Identifier: 158338836

GI number: 158338836

Start: 5827105

End: 5828190

Strand: Direct

Name: gmd [H]

Synonym: AM1_5749

Alternate gene names: 158338836

Gene position: 5827105-5828190 (Clockwise)

Preceding gene: 158338835

Following gene: 158338837

Centisome position: 89.6

GC content: 46.59

Gene sequence:

>1086_bases
ATGGCAAAAGTCGCTCTCATTACTGGCATTACGGGTCAAGATGGAGCCTATCTGGCAGAGCTATTATTGGCCAAAGGCTA
TCACGTTCATGGGATTAAACGACGCGCTTCACTCTTCAATACAGATCGTATTGATCACTTATACCAAGATCCCCATGAGA
AGGATCGACGCTTTTTCCTTCACTACGGTGACTTGACCGATTCAACCAATCTTATTCGGATTGTGCAGCAAGTCCAGCCC
GACGAAATTTATAACTTAGCGGCTCAAAGCCATGTGGCGGTTTCGTTTGAGACGCCAGAATATACCGCCAATGTTGATGG
CCTTGGCACCTTGAGATTCTTGGAAGCCATTCGCATCCTAGGCTTGGAGAAAAAAACGAAGTTTTATCAGGCCTCTACCT
CTGAATTGTATGGTTTAGTGCAAGAAGTGCCTCAAACGGAGAAAACCCCCTTTTACCCTCGCTCTCCTTACGCTGTTGCT
AAACTCTATGCCTACTGGATTACGGTGAATTACCGAGAAGCCTATGGCATGTATGCCTGTAATGGCATTTTGTTTAACCA
TGAATCTCCACTTCGGGGTGAAACCTTTGTCACCCGGAAGATCACCCGTGCGATCGCACGAATTTACCTGAAAAAGCAAA
AGTGCCTATTTTTAGGAAATATGGACTCCTTAAGGGATTGGGGCCATGCTCGGGACTATGTGGAAATGCAGTGGTTAATG
CTTCAGCAAGACCAGCCTGAAGATTTTGTGATTGCCACGGGACAACAATACAGCGTCCGCCATTTTGTGACGATTGCCTG
TAAAGAAGTCGGCATTGACATTCGCTGGGAAGGGGAAGGTCTTGACGAAAAAGGCTATGACCAGGAGGGGAATTGTATCG
TCGCAGTCGATCCCCGTTACTATCGCCCCACAGAAGTAGATACCTTATTAGGGAGTCCCGAGAAAGCGAAGGAAAAACTC
AAATGGGTTCCCAAAGTTAGCTTTCCAGAGCTAGTCCAGGAAATGATGGCAGAAGATTTGAAAGCCGCTAAGCGGGATAA
GATTATTGAGGATCACGGCTATCCTGTTCCTCTCGTCCATGAATAA

Upstream 100 bases:

>100_bases
ATTGACAGTAGGAACTCAATCCTTGAATCCATTTGTCAATAGGATCCGCGCCATTCATTTCTATTAGCAGCACAGTTGAA
TCACACATAGGATAAATTTC

Downstream 100 bases:

>100_bases
ATCTGCGTTTTAGGCTAAATTTTAAGCCTGTGGATAGGGGCGATTCCCAGTGAACATTATTTGGTAATGAAAGTACTGCT
AATACATGGCCTGGGACGTT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAKVALITGITGQDGAYLAELLLAKGYHVHGIKRRASLFNTDRIDHLYQDPHEKDRRFFLHYGDLTDSTNLIRIVQQVQP
DEIYNLAAQSHVAVSFETPEYTANVDGLGTLRFLEAIRILGLEKKTKFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVA
KLYAYWITVNYREAYGMYACNGILFNHESPLRGETFVTRKITRAIARIYLKKQKCLFLGNMDSLRDWGHARDYVEMQWLM
LQQDQPEDFVIATGQQYSVRHFVTIACKEVGIDIRWEGEGLDEKGYDQEGNCIVAVDPRYYRPTEVDTLLGSPEKAKEKL
KWVPKVSFPELVQEMMAEDLKAAKRDKIIEDHGYPVPLVHE

Sequences:

>Translated_361_residues
MAKVALITGITGQDGAYLAELLLAKGYHVHGIKRRASLFNTDRIDHLYQDPHEKDRRFFLHYGDLTDSTNLIRIVQQVQP
DEIYNLAAQSHVAVSFETPEYTANVDGLGTLRFLEAIRILGLEKKTKFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVA
KLYAYWITVNYREAYGMYACNGILFNHESPLRGETFVTRKITRAIARIYLKKQKCLFLGNMDSLRDWGHARDYVEMQWLM
LQQDQPEDFVIATGQQYSVRHFVTIACKEVGIDIRWEGEGLDEKGYDQEGNCIVAVDPRYYRPTEVDTLLGSPEKAKEKL
KWVPKVSFPELVQEMMAEDLKAAKRDKIIEDHGYPVPLVHE
>Mature_360_residues
AKVALITGITGQDGAYLAELLLAKGYHVHGIKRRASLFNTDRIDHLYQDPHEKDRRFFLHYGDLTDSTNLIRIVQQVQPD
EIYNLAAQSHVAVSFETPEYTANVDGLGTLRFLEAIRILGLEKKTKFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAK
LYAYWITVNYREAYGMYACNGILFNHESPLRGETFVTRKITRAIARIYLKKQKCLFLGNMDSLRDWGHARDYVEMQWLML
QQDQPEDFVIATGQQYSVRHFVTIACKEVGIDIRWEGEGLDEKGYDQEGNCIVAVDPRYYRPTEVDTLLGSPEKAKEKLK
WVPKVSFPELVQEMMAEDLKAAKRDKIIEDHGYPVPLVHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:
GO:0003824: GDP-mannose 4,6-dehydratase
GO:0005488: GDP-mannose 4,6-dehydratase
GO:0005622: GDP-mannose 4,6-dehydratase
GO:0008446: GDP-mannose 4,6-dehydratase
GO:0019673: GDP-mannose 4,6-dehydratase
GO:0044237: GDP-mannose 4,6-dehydratase
GO:0050662: GDP-mannose 4,6-dehydratase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=346, Percent_Identity=62.1387283236994, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1788366, Length=372, Percent_Identity=70.1612903225807, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI48994969, Length=193, Percent_Identity=29.5336787564767, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788353, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=64.6551724137931, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17539424, Length=348, Percent_Identity=64.367816091954, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI133901788, Length=348, Percent_Identity=64.367816091954, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17539422, Length=348, Percent_Identity=64.367816091954, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI133901790, Length=348, Percent_Identity=64.367816091954, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI133901786, Length=348, Percent_Identity=64.367816091954, Blast_Score=483, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=62.3563218390805, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41555; Mature: 41424

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVALITGITGQDGAYLAELLLAKGYHVHGIKRRASLFNTDRIDHLYQDPHEKDRRFFL
CCEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHCCCCCCCCEEEE
HYGDLTDSTNLIRIVQQVQPDEIYNLAAQSHVAVSFETPEYTANVDGLGTLRFLEAIRIL
EECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEECCCEEECCCCCHHHHHHHHHHHH
GLEKKTKFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWITVNYREAYGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEE
NGILFNHESPLRGETFVTRKITRAIARIYLKKQKCLFLGNMDSLRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCHHHHHEEEE
LQQDQPEDFVIATGQQYSVRHFVTIACKEVGIDIRWEGEGLDEKGYDQEGNCIVAVDPRY
ECCCCCCCEEEECCCCCCHHHEEEEEEHHHCEEEEECCCCCCCCCCCCCCCEEEEECCCC
YRPTEVDTLLGSPEKAKEKLKWVPKVSFPELVQEMMAEDLKAAKRDKIIEDHGYPVPLVH
CCCCCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
E
C
>Mature Secondary Structure 
AKVALITGITGQDGAYLAELLLAKGYHVHGIKRRASLFNTDRIDHLYQDPHEKDRRFFL
CEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHCCCCCCCCEEEE
HYGDLTDSTNLIRIVQQVQPDEIYNLAAQSHVAVSFETPEYTANVDGLGTLRFLEAIRIL
EECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEECCCEEECCCCCHHHHHHHHHHHH
GLEKKTKFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWITVNYREAYGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEE
NGILFNHESPLRGETFVTRKITRAIARIYLKKQKCLFLGNMDSLRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCHHHHHEEEE
LQQDQPEDFVIATGQQYSVRHFVTIACKEVGIDIRWEGEGLDEKGYDQEGNCIVAVDPRY
ECCCCCCCEEEECCCCCCHHHEEEEEEHHHCEEEEECCCCCCCCCCCCCCCEEEEECCCC
YRPTEVDTLLGSPEKAKEKLKWVPKVSFPELVQEMMAEDLKAAKRDKIIEDHGYPVPLVH
CCCCCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]