Click here to switch to the map view.

The map label for this gene is gyrA [H]

Identifier: 158338824

GI number: 158338824

Start: 5815744

End: 5818218

Strand: Reverse

Name: gyrA [H]

Synonym: AM1_5737

Alternate gene names: 158338824

Gene position: 5818218-5815744 (Counterclockwise)

Preceding gene: 158338826

Following gene: 158338823

Centisome position: 89.46

GC content: 50.55

Gene sequence:

>2475_bases
ATGTCCAAGCAACTCAATCTTCTCTCATCCGGTAAAGTCATCGCCACGGCTTTGCATACCGAGATGGAGCAGTCCTACTT
GGAATATGCCATGAGCGTGATCGTCGGGCGCGCATTACCCGATGTCCGGGATGGATTAAAGCCCGTGCATCGGCGCATTC
TCTACGCCATGCACGAACTAGGACTAACGCCAGACCGTCCCTACCGTAAATGTGCCAGAGTCGTGGGGGATGTGCTGGGT
AAATATCATCCCCACGGCGATCAGGCGGTTTATGATGCCTTAGTCAGGCTGGTTCAGGATTTTTCGAGTCGGTATCCTCT
ATTGGCGGGGCATGGCAACTTTGGCTCTGTGGATAACGATCCACCGGCTGCCATGCGATACACGGAAACACGCTTGTCTC
CCATTGGCAATCAGTCCCTTTTAGAAGAAATTGGCGAATCCACCGTTGAATTTGCCGCCAACTTTGATAACTCCCAGCAA
GAACCCACCGTCTTACCGGCCCAACTCCCCACCCTTCTCCTCAACGGCTGTTCGGGGATTGCAGTGGGCATGGCGACCAA
TATCCCTCCCCATAATTTGGGAGAGATTGTTGATGCTCTGGTGGCGCTGATTGATTCTCCAGACATGACCTTAGAAAAAT
TAATGGCCTTGGTACCTGGGCCGGACTTTCCCACAGGGGGGGAGATTGTCGGCAAGCAGGGAATTATCGACGCCTACACC
AATGGTCGGGGAAGCATCACGGTGCGAGCTGTGACCTCCATTGAGGAAATCCAGCCGGGCCGAGGCCGACATCGCCGTCC
CGTGATTATTGTGACGGAACTGCCCTATCAGGTAAATAAGGCAGGCTGTATTGAAAAAATCGCTAGCTTAATTAACCAGG
GTCGGATTGAAGGCATTGCAGACCTGCGCGATGAAAGCGATCGCGAAGGGATGCGAATTGTGATTGAGTTGAAGCGGGAA
GCGCAACCCCCGGCTGTGCTAGAGCAACTTTATCGGCTTACTCCTCTGCAATCAAATTTTGGGGTGATTATGTTGGCCCT
GGTCAATGGCGTGCCCTTGCAGCTGTCATTGAAGGAGGTATTGCAGAAATTCCTTGATTTCCGAGAACAGACCTTAACCC
GCCGATATCAAAATCAGTTAGAGCAAGCTCAAAGCCGCCAACATTTAGTCGAAGGACTCATTAAAGCCCTCGACCATTTA
GATGACCTAATCGAGATTCTTCGCCATGCCCCCGATGGGACGACGGCAAAAGTTCAGCTCAAATCTCAATTCCAATTGAG
TGGCAGTCAGGCCGATGCCATCTTAGCGATGCCGATGCGGCGATTGACGGGGTTAGAGCAACAAAATCTCCGGCAAGAAT
TTGATGATTTGGCCCAGGAAATTGGCGATTTAGAACGATTATTGGGGAATCGCCATGAACTCTTAAAAGCCTTAAAGAAA
GATCTACGAGCCCTGAAAAAGCAATATAGCGATCCGCGGCGGACTGTTATCCATGGAGCCGCAGCTAAAACCGAGATATT
CGCCGCACCGGTTAGTGATGAATCTGTGATTGTGGAGCTGAACCAGCGGGGGTATGCTCGCTGCATGCGCGTATCTAAGC
GTAAAAATAATGCACCTGATTTGTCTGGGGAGCTAGCAGACGTTTCAACAGATTTGACCATTTTTCGGCATACGACAGAA
CTGACAACAGACCTACTGTTGTTTACGGGCAGTGGCAAGGCCTATGGCGTCGGGTTACAGTCCATCCCATTTACCTCAAG
CAAAGGCAAGGGGAAACCCTTAGTCACCCTGCTGCCCGATTCGGGACGCGATGACGAAATTATTGCCCGCTTTCTCAGCA
CTGAAATTTCCGATACGAGTCAGCTTATCCTTTTGTCTCAACAGGGCAAAATTAAGCGCCTGCCCATGAGCGAGTTTGCC
GAGATGACGAGTCGGGGATTGGGGGCTGCCAAGATTAAATCGGGGGATCAGTTGGGCTGGGTGGTTAAAGCTGAGCAGGA
AACCAATGTTGCGATCGCAACCTCTTCAGGACGCCTGTTAAATATTCCCATAACCGAGATTCCTGAAATGGGCCGCACTG
CCCAGGGCAATCAAGTGTTTCGGTTAAGAAAGCAAGAATCGATTGTCGGATTAGCAGCAGTTCGAGACGATGCAGATTTG
CTGCTGATATCTAGTAAGGGCTATTGCAAACGATTACCCATTGATTTGTTGCGGTTAGGGAAAGTGGGGGATCTAGGCAC
TCAAGCAATGCAGTTTATGATCAAATCGGATGGATTGATCGCGTTGTTGCCAGCCAATCCCAATCAAGATGTGATTGTCC
TCAGTAATCAGGAGCGAGCCGCTCGGTTAAATATTGAGACTTTGCCCATGTGGGGGCAGGATGGTCCTGGGACTCAATTA
CTCAAAACCAAGCGGGGAGAGGTTTTGGAAAACACCGTAGTCATACCGAAGGCTGATACTGAGAAGGAGACATAA

Upstream 100 bases:

>100_bases
GTGCATTGTAGCCTGATGCCTTTTCGCGAGGGTTGGGCAGAATTGGATTGTCCCCTTCCTGTACCCCGTTGATTTCGCCA
GATCTGAAGGAACCTCACTC

Downstream 100 bases:

>100_bases
ATCATTTTGCCGACCCAGGGTGATGCACAAAGCAGTATCTTCCCTTCGTCCAAGCTCTATGAGGGTATAGACGACTCAGA
AGCCAATTAGATATTTATTA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MSKQLNLLSSGKVIATALHTEMEQSYLEYAMSVIVGRALPDVRDGLKPVHRRILYAMHELGLTPDRPYRKCARVVGDVLG
KYHPHGDQAVYDALVRLVQDFSSRYPLLAGHGNFGSVDNDPPAAMRYTETRLSPIGNQSLLEEIGESTVEFAANFDNSQQ
EPTVLPAQLPTLLLNGCSGIAVGMATNIPPHNLGEIVDALVALIDSPDMTLEKLMALVPGPDFPTGGEIVGKQGIIDAYT
NGRGSITVRAVTSIEEIQPGRGRHRRPVIIVTELPYQVNKAGCIEKIASLINQGRIEGIADLRDESDREGMRIVIELKRE
AQPPAVLEQLYRLTPLQSNFGVIMLALVNGVPLQLSLKEVLQKFLDFREQTLTRRYQNQLEQAQSRQHLVEGLIKALDHL
DDLIEILRHAPDGTTAKVQLKSQFQLSGSQADAILAMPMRRLTGLEQQNLRQEFDDLAQEIGDLERLLGNRHELLKALKK
DLRALKKQYSDPRRTVIHGAAAKTEIFAAPVSDESVIVELNQRGYARCMRVSKRKNNAPDLSGELADVSTDLTIFRHTTE
LTTDLLLFTGSGKAYGVGLQSIPFTSSKGKGKPLVTLLPDSGRDDEIIARFLSTEISDTSQLILLSQQGKIKRLPMSEFA
EMTSRGLGAAKIKSGDQLGWVVKAEQETNVAIATSSGRLLNIPITEIPEMGRTAQGNQVFRLRKQESIVGLAAVRDDADL
LLISSKGYCKRLPIDLLRLGKVGDLGTQAMQFMIKSDGLIALLPANPNQDVIVLSNQERAARLNIETLPMWGQDGPGTQL
LKTKRGEVLENTVVIPKADTEKET

Sequences:

>Translated_824_residues
MSKQLNLLSSGKVIATALHTEMEQSYLEYAMSVIVGRALPDVRDGLKPVHRRILYAMHELGLTPDRPYRKCARVVGDVLG
KYHPHGDQAVYDALVRLVQDFSSRYPLLAGHGNFGSVDNDPPAAMRYTETRLSPIGNQSLLEEIGESTVEFAANFDNSQQ
EPTVLPAQLPTLLLNGCSGIAVGMATNIPPHNLGEIVDALVALIDSPDMTLEKLMALVPGPDFPTGGEIVGKQGIIDAYT
NGRGSITVRAVTSIEEIQPGRGRHRRPVIIVTELPYQVNKAGCIEKIASLINQGRIEGIADLRDESDREGMRIVIELKRE
AQPPAVLEQLYRLTPLQSNFGVIMLALVNGVPLQLSLKEVLQKFLDFREQTLTRRYQNQLEQAQSRQHLVEGLIKALDHL
DDLIEILRHAPDGTTAKVQLKSQFQLSGSQADAILAMPMRRLTGLEQQNLRQEFDDLAQEIGDLERLLGNRHELLKALKK
DLRALKKQYSDPRRTVIHGAAAKTEIFAAPVSDESVIVELNQRGYARCMRVSKRKNNAPDLSGELADVSTDLTIFRHTTE
LTTDLLLFTGSGKAYGVGLQSIPFTSSKGKGKPLVTLLPDSGRDDEIIARFLSTEISDTSQLILLSQQGKIKRLPMSEFA
EMTSRGLGAAKIKSGDQLGWVVKAEQETNVAIATSSGRLLNIPITEIPEMGRTAQGNQVFRLRKQESIVGLAAVRDDADL
LLISSKGYCKRLPIDLLRLGKVGDLGTQAMQFMIKSDGLIALLPANPNQDVIVLSNQERAARLNIETLPMWGQDGPGTQL
LKTKRGEVLENTVVIPKADTEKET
>Mature_823_residues
SKQLNLLSSGKVIATALHTEMEQSYLEYAMSVIVGRALPDVRDGLKPVHRRILYAMHELGLTPDRPYRKCARVVGDVLGK
YHPHGDQAVYDALVRLVQDFSSRYPLLAGHGNFGSVDNDPPAAMRYTETRLSPIGNQSLLEEIGESTVEFAANFDNSQQE
PTVLPAQLPTLLLNGCSGIAVGMATNIPPHNLGEIVDALVALIDSPDMTLEKLMALVPGPDFPTGGEIVGKQGIIDAYTN
GRGSITVRAVTSIEEIQPGRGRHRRPVIIVTELPYQVNKAGCIEKIASLINQGRIEGIADLRDESDREGMRIVIELKREA
QPPAVLEQLYRLTPLQSNFGVIMLALVNGVPLQLSLKEVLQKFLDFREQTLTRRYQNQLEQAQSRQHLVEGLIKALDHLD
DLIEILRHAPDGTTAKVQLKSQFQLSGSQADAILAMPMRRLTGLEQQNLRQEFDDLAQEIGDLERLLGNRHELLKALKKD
LRALKKQYSDPRRTVIHGAAAKTEIFAAPVSDESVIVELNQRGYARCMRVSKRKNNAPDLSGELADVSTDLTIFRHTTEL
TTDLLLFTGSGKAYGVGLQSIPFTSSKGKGKPLVTLLPDSGRDDEIIARFLSTEISDTSQLILLSQQGKIKRLPMSEFAE
MTSRGLGAAKIKSGDQLGWVVKAEQETNVAIATSSGRLLNIPITEIPEMGRTAQGNQVFRLRKQESIVGLAAVRDDADLL
LISSKGYCKRLPIDLLRLGKVGDLGTQAMQFMIKSDGLIALLPANPNQDVIVLSNQERAARLNIETLPMWGQDGPGTQLL
KTKRGEVLENTVVIPKADTEKET

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=523, Percent_Identity=22.3709369024857, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI19913406, Length=344, Percent_Identity=25, Blast_Score=76, Evalue=2e-13,
Organism=Escherichia coli, GI1788562, Length=826, Percent_Identity=37.2881355932203, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789396, Length=709, Percent_Identity=36.5303244005642, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=377, Percent_Identity=25.9946949602122, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI212645845, Length=466, Percent_Identity=25.1072961373391, Blast_Score=83, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI212645657, Length=376, Percent_Identity=25, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=188, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90582; Mature: 90450

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQLNLLSSGKVIATALHTEMEQSYLEYAMSVIVGRALPDVRDGLKPVHRRILYAMHEL
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLTPDRPYRKCARVVGDVLGKYHPHGDQAVYDALVRLVQDFSSRYPLLAGHGNFGSVDND
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
PPAAMRYTETRLSPIGNQSLLEEIGESTVEFAANFDNSQQEPTVLPAQLPTLLLNGCSGI
CCCHHEEHHHHCCCCCCHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCE
AVGMATNIPPHNLGEIVDALVALIDSPDMTLEKLMALVPGPDFPTGGEIVGKQGIIDAYT
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NGRGSITVRAVTSIEEIQPGRGRHRRPVIIVTELPYQVNKAGCIEKIASLINQGRIEGIA
CCCCCEEEEEECCHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHH
DLRDESDREGMRIVIELKREAQPPAVLEQLYRLTPLQSNFGVIMLALVNGVPLQLSLKEV
HHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHCCCCEEEHHHHH
LQKFLDFREQTLTRRYQNQLEQAQSRQHLVEGLIKALDHLDDLIEILRHAPDGTTAKVQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
KSQFQLSGSQADAILAMPMRRLTGLEQQNLRQEFDDLAQEIGDLERLLGNRHELLKALKK
EEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLRALKKQYSDPRRTVIHGAAAKTEIFAAPVSDESVIVELNQRGYARCMRVSKRKNNAPD
HHHHHHHHCCCCHHHEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCC
LSGELADVSTDLTIFRHTTELTTDLLLFTGSGKAYGVGLQSIPFTSSKGKGKPLVTLLPD
CCCCHHHCCCCEEEEEEHHHHCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECCC
SGRDDEIIARFLSTEISDTSQLILLSQQGKIKRLPMSEFAEMTSRGLGAAKIKSGDQLGW
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEEE
VVKAEQETNVAIATSSGRLLNIPITEIPEMGRTAQGNQVFRLRKQESIVGLAAVRDDADL
EEEECCCCCEEEEECCCCEEECCHHHCCCCCCCCCCCCEEEEEHHCCEEEEEEECCCCCE
LLISSKGYCKRLPIDLLRLGKVGDLGTQAMQFMIKSDGLIALLPANPNQDVIVLSNQERA
EEEECCCCCHHCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCC
ARLNIETLPMWGQDGPGTQLLKTKRGEVLENTVVIPKADTEKET
EEECEEECCCCCCCCCCHHHHHHHCCCHHHCCEEECCCCCCCCC
>Mature Secondary Structure 
SKQLNLLSSGKVIATALHTEMEQSYLEYAMSVIVGRALPDVRDGLKPVHRRILYAMHEL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLTPDRPYRKCARVVGDVLGKYHPHGDQAVYDALVRLVQDFSSRYPLLAGHGNFGSVDND
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
PPAAMRYTETRLSPIGNQSLLEEIGESTVEFAANFDNSQQEPTVLPAQLPTLLLNGCSGI
CCCHHEEHHHHCCCCCCHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCE
AVGMATNIPPHNLGEIVDALVALIDSPDMTLEKLMALVPGPDFPTGGEIVGKQGIIDAYT
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NGRGSITVRAVTSIEEIQPGRGRHRRPVIIVTELPYQVNKAGCIEKIASLINQGRIEGIA
CCCCCEEEEEECCHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHH
DLRDESDREGMRIVIELKREAQPPAVLEQLYRLTPLQSNFGVIMLALVNGVPLQLSLKEV
HHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHCCCCEEEHHHHH
LQKFLDFREQTLTRRYQNQLEQAQSRQHLVEGLIKALDHLDDLIEILRHAPDGTTAKVQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
KSQFQLSGSQADAILAMPMRRLTGLEQQNLRQEFDDLAQEIGDLERLLGNRHELLKALKK
EEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLRALKKQYSDPRRTVIHGAAAKTEIFAAPVSDESVIVELNQRGYARCMRVSKRKNNAPD
HHHHHHHHCCCCHHHEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCC
LSGELADVSTDLTIFRHTTELTTDLLLFTGSGKAYGVGLQSIPFTSSKGKGKPLVTLLPD
CCCCHHHCCCCEEEEEEHHHHCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECCC
SGRDDEIIARFLSTEISDTSQLILLSQQGKIKRLPMSEFAEMTSRGLGAAKIKSGDQLGW
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCEEE
VVKAEQETNVAIATSSGRLLNIPITEIPEMGRTAQGNQVFRLRKQESIVGLAAVRDDADL
EEEECCCCCEEEEECCCCEEECCHHHCCCCCCCCCCCCEEEEEHHCCEEEEEEECCCCCE
LLISSKGYCKRLPIDLLRLGKVGDLGTQAMQFMIKSDGLIALLPANPNQDVIVLSNQERA
EEEECCCCCHHCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCC
ARLNIETLPMWGQDGPGTQLLKTKRGEVLENTVVIPKADTEKET
EEECEEECCCCCCCCCCHHHHHHHCCCHHHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]