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The map label for this gene is metK

Identifier: 158338791

GI number: 158338791

Start: 5774799

End: 5776055

Strand: Reverse

Name: metK

Synonym: AM1_5701

Alternate gene names: 158338791

Gene position: 5776055-5774799 (Counterclockwise)

Preceding gene: 158338792

Following gene: 158338789

Centisome position: 88.81

GC content: 51.15

Gene sequence:

>1257_bases
TTGCCTAAGCGTTATCTCTTCACTTCAGAATCTGTGACTGAAGGCCATCCCGATAAGGTTTGTGATCAAATTTCTGACAC
GATTCTTGATGCTCTACTTTCTCAAGACCCCGCTAGCCGGGTTGCGGCTGAAGTAGTGGTTAACACTGGCCTCGTTCTCT
TAACTGGAGAAATCACCACGAAAGCCCAAGTCAACTTTGTTGATTTGGTTCGTCAGAAAATTACTGAAATTGGCTATACC
GATTCCGATAATGGCTTTGCCGCCAATAGCTGTTCTGTCCTGGTTGCCCTAGATGAGCAATCTCCAGATATTGCCCAAGG
GGTTGACTCCGCCCAAGAAACCCGCGAAGAGAAAAGCGATCAAGAGCTGGATGCCATTGGGGCCGGTGACCAAGGCTTAA
TGTTCGGGTTTGCCTGCAACGAGACCCCTGAACTGATGCCATTGCCGATTAGCCTCGCCCATCGAGTCACTCGCCGCTTG
GCTGCCGTGCGCAAAACCCAACTGGGCTATTTAAGACCCGATGGTAAATCTCAGGTTACAGTCACCTACGAAGATGGGCG
TCCCGTCGGCATTGATACAATTCTTGTTTCGACCCAGCATGATGCCACCATTGGTGACATTACTGACCCTGCCGCAGTAC
AGGCCAAGATTAAGGAAGATCTGTGGAATGCGGTTGTACTCCCTGTCTTTGCTGATATTGACATCAAGCCCGACGACAAT
ACTCGATTCCTTGTCAATCCCACTGGCCAATTTGTTGTAGGGGGTCCCCAAGGCGATGCCGGACTCACGGGACGGAAGAT
TATTGTAGATACCTACGGAGGATATTCCCGTCATGGCGGTGGTGCTTTTTCCGGTAAGGATCCCACAAAAGTAGACCGCA
GTGCCGCCTATGCCTGCCGATATGTAGCTAAGAATATCGTTGCTGCCGGGCTGGCCGAGAAGTGTGAAGTTCAGCTCAGC
TACGCCATTGGTGTTGCTCGCCCCGTTAGCATTTTGATCGAGACGTTTGGCACTGGCAAGGTGGATGAAGATCGTCTGCT
GCAGGTTGTTCAAGAAAATTTCGAACTTCGTCCAGCGGGGTTGATTCAAACCTTCGGCTTAACCAAACTCCCAGGTGAGC
GAGGCGGTCGGTTCTACCAAGATGTGGCGGCCTATGGCCATTTTGGTCGTACCGATTTAGATCTGCCTTGGGAAGCCACC
GATAAAGCAGATCTGCTCAAGCAAGCCTTGAGTCCTGCCCTATCTGGCAACGCCTAA

Upstream 100 bases:

>100_bases
CTCAAAGTCTATTCAGGAGAGAATGCTATAATTCTCTGGACTCACAGGAATTTTTGCTCATTATTGTTCTCTCGCTTTGC
TCCACAGGAGGATCAAGATC

Downstream 100 bases:

>100_bases
CGAATCTCTTCATAAAAGCGCTTTAAAAATTCGGCGGGTAAACCCATAAACCACAGCCAACCTATCGAGTGATAGATGAA
TGTGGCTTTCCAAGCCCCCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPKRYLFTSESVTEGHPDKVCDQISDTILDALLSQDPASRVAAEVVVNTGLVLLTGEITTKAQVNFVDLVRQKITEIGYT
DSDNGFAANSCSVLVALDEQSPDIAQGVDSAQETREEKSDQELDAIGAGDQGLMFGFACNETPELMPLPISLAHRVTRRL
AAVRKTQLGYLRPDGKSQVTVTYEDGRPVGIDTILVSTQHDATIGDITDPAAVQAKIKEDLWNAVVLPVFADIDIKPDDN
TRFLVNPTGQFVVGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYACRYVAKNIVAAGLAEKCEVQLS
YAIGVARPVSILIETFGTGKVDEDRLLQVVQENFELRPAGLIQTFGLTKLPGERGGRFYQDVAAYGHFGRTDLDLPWEAT
DKADLLKQALSPALSGNA

Sequences:

>Translated_418_residues
MPKRYLFTSESVTEGHPDKVCDQISDTILDALLSQDPASRVAAEVVVNTGLVLLTGEITTKAQVNFVDLVRQKITEIGYT
DSDNGFAANSCSVLVALDEQSPDIAQGVDSAQETREEKSDQELDAIGAGDQGLMFGFACNETPELMPLPISLAHRVTRRL
AAVRKTQLGYLRPDGKSQVTVTYEDGRPVGIDTILVSTQHDATIGDITDPAAVQAKIKEDLWNAVVLPVFADIDIKPDDN
TRFLVNPTGQFVVGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYACRYVAKNIVAAGLAEKCEVQLS
YAIGVARPVSILIETFGTGKVDEDRLLQVVQENFELRPAGLIQTFGLTKLPGERGGRFYQDVAAYGHFGRTDLDLPWEAT
DKADLLKQALSPALSGNA
>Mature_417_residues
PKRYLFTSESVTEGHPDKVCDQISDTILDALLSQDPASRVAAEVVVNTGLVLLTGEITTKAQVNFVDLVRQKITEIGYTD
SDNGFAANSCSVLVALDEQSPDIAQGVDSAQETREEKSDQELDAIGAGDQGLMFGFACNETPELMPLPISLAHRVTRRLA
AVRKTQLGYLRPDGKSQVTVTYEDGRPVGIDTILVSTQHDATIGDITDPAAVQAKIKEDLWNAVVLPVFADIDIKPDDNT
RFLVNPTGQFVVGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYACRYVAKNIVAAGLAEKCEVQLSY
AIGVARPVSILIETFGTGKVDEDRLLQVVQENFELRPAGLIQTFGLTKLPGERGGRFYQDVAAYGHFGRTDLDLPWEATD
KADLLKQALSPALSGNA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:
GO:0000166: S-adenosylmethionine synthase
GO:0004478: S-adenosylmethionine synthase
GO:0005524: S-adenosylmethionine synthase
GO:0005737: S-adenosylmethionine synthase
GO:0006556: S-adenosylmethionine synthase
GO:0006730: S-adenosylmethionine synthase
GO:0016740: S-adenosylmethionine synthase
GO:0046872: S-adenosylmethionine synthase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=403, Percent_Identity=51.3647642679901, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI4557737, Length=403, Percent_Identity=52.605459057072, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1789311, Length=407, Percent_Identity=56.7567567567568, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17538498, Length=425, Percent_Identity=48.9411764705882, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=420, Percent_Identity=49.047619047619, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538494, Length=402, Percent_Identity=50, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551082, Length=422, Percent_Identity=48.1042654028436, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=402, Percent_Identity=44.7761194029851, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI32565911, Length=374, Percent_Identity=46.524064171123, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25145635, Length=373, Percent_Identity=46.6487935656836, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=328, Percent_Identity=48.1707317073171, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=60.2409638554217, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=71.7948717948718, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=403, Percent_Identity=50.1240694789082, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6320710, Length=408, Percent_Identity=49.2647058823529, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45552159, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580529, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580537, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580533, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580535, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580531, Length=399, Percent_Identity=48.8721804511278, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580527, Length=399, Percent_Identity=48.3709273182957, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24580525, Length=399, Percent_Identity=48.3709273182957, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24580541, Length=386, Percent_Identity=47.1502590673575, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24580539, Length=184, Percent_Identity=42.3913043478261, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ACAM1 (B0CG96)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519968.1
- ProteinModelPortal:   B0CG96
- SMR:   B0CG96
- GeneID:   5684490
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5701
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   AMAR329726:AM1_5701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44887; Mature: 44756

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRYLFTSESVTEGHPDKVCDQISDTILDALLSQDPASRVAAEVVVNTGLVLLTGEITT
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCC
KAQVNFVDLVRQKITEIGYTDSDNGFAANSCSVLVALDEQSPDIAQGVDSAQETREEKSD
CEECHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCHHHHHCCHHHHHHHHHCC
QELDAIGAGDQGLMFGFACNETPELMPLPISLAHRVTRRLAAVRKTQLGYLRPDGKSQVT
CHHHHCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCEEE
VTYEDGRPVGIDTILVSTQHDATIGDITDPAAVQAKIKEDLWNAVVLPVFADIDIKPDDN
EEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCC
TRFLVNPTGQFVVGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYACR
CEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YVAKNIVAAGLAEKCEVQLSYAIGVARPVSILIETFGTGKVDEDRLLQVVQENFELRPAG
HHHHHHHHHCCHHHHEEEEEEHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCH
LIQTFGLTKLPGERGGRFYQDVAAYGHFGRTDLDLPWEATDKADLLKQALSPALSGNA
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKRYLFTSESVTEGHPDKVCDQISDTILDALLSQDPASRVAAEVVVNTGLVLLTGEITT
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCC
KAQVNFVDLVRQKITEIGYTDSDNGFAANSCSVLVALDEQSPDIAQGVDSAQETREEKSD
CEECHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCHHHHHCCHHHHHHHHHCC
QELDAIGAGDQGLMFGFACNETPELMPLPISLAHRVTRRLAAVRKTQLGYLRPDGKSQVT
CHHHHCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCEEE
VTYEDGRPVGIDTILVSTQHDATIGDITDPAAVQAKIKEDLWNAVVLPVFADIDIKPDDN
EEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCC
TRFLVNPTGQFVVGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYACR
CEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YVAKNIVAAGLAEKCEVQLSYAIGVARPVSILIETFGTGKVDEDRLLQVVQENFELRPAG
HHHHHHHHHCCHHHHEEEEEEHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCH
LIQTFGLTKLPGERGGRFYQDVAAYGHFGRTDLDLPWEATDKADLLKQALSPALSGNA
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA