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The map label for this gene is barA [H]

Identifier: 158338777

GI number: 158338777

Start: 5753934

End: 5756414

Strand: Reverse

Name: barA [H]

Synonym: AM1_5688

Alternate gene names: 158338777

Gene position: 5756414-5753934 (Counterclockwise)

Preceding gene: 158338783

Following gene: 158338773

Centisome position: 88.51

GC content: 50.67

Gene sequence:

>2481_bases
ATGCCAATGCCCCACCAGCCTGCTGATCCATTCGACATTGCATCCCCTTTGTCCTTGGCCAATCCCTTCGCAGCCTTTAT
CGACCAAACGGTTGATATTTTTGTGGCCTACGACCAGGATCTGCGAATTATCACCATCAACCGCATCGGGGCGCAGCGGC
TGCAGCGACCCTATGAACAAATCCTGGGACAAACCAATCAGCAACTCTTTGGGGCACAAGCAGAACTGATTGATCCGTTT
CTGATGTCGGCCTTAGAAACTGGGGAACGAGTCTTTGCTGAGCAGGAACTTGTGTTTAATCAGCAACGTTACTACTACGA
CACGATTTATACCCCCACCACAGATGCCAGGACAGGCGAACGGCAGGTGTGGGGAATTTGCCGAGATATCACCATCAAAA
AGGATCTACAAAAGAAGCGGGAAGCCCTCCTCAAACAGCAAACCATCCAGATCGAGGAGCGCTTTCGGACATCGTTTAAT
AATGCGACCATCGCCAAGGCATTAATTGCCCTAGATGGGTCATGGCTTCAAGTTAACCCAGCCCTTAGTCAACTCCTCGG
CTATGACCCAACTGAATTACTAAAGTTACGCATTGAAGATCTCACTCACCCGGAGGATCAAGACATTGATCGGCATCAAG
TCCGCCAGCTACTCATCGGCCAAACATCGAGCTACCAAATTGAGAAGCGGTTCCTCCATCGTTTAGGCCACACTCTGTCC
GTCCTCTTAAGCGTCGCCCTTATCCTCAATTCTCAAGGCAAACCCTCCTACTTCATTGCTGAGATGCAGGATATTACCCA
ACGCAAACAGGCCGAGGCGGCCCTGTACCTCCAGCTCCAAAAGACCCTACAGCTGCAAACTGCCCTGCAAACCAAAAACC
AGGCCCTGCTAGAAAGTAGCCAAGCCGCCCAAGCGGCAAACCTAGCCAAAAGTGAGTTTCTAGCCATGATTAGCCATGAA
ATTCGCACGCCCATGAATGCCGTCATCGGCATGACGGATTTACTGAAAGATACTCACCTCGATCCCCATCAGCAGGATTT
TGTGGAAACGATTCGAACCAGCGGTGAATCACTCCTCAATATCATTAACGACATTCTCGATTTCTCCAAGATCGAGTCAG
GGAAACTAGAACTAGAACTGCACCCCTTCCCCATCTCCACTTGTGTGGAAGAAGCCTTAGATATTGTTAATTCCAAAGCG
ACAGCCAAAGGGCTGGAACTAGCGTATTTGATTGAACCGGGAACACCCAACCATATCCAGGGGGATGCCAATCGCCTCCG
ACAAATCCTCGTCAACTTGCTGGGGAATGCGGTGAAATTCACGGCTGATGGCGAAGTGATTGTGACCATCTCCGCCCGAG
ATGTTGCCACTGCTGAGATGACCCCAGAAGTCCCTTTATCCCTCCATGAACTCCTCTTTGAAGTCAAAGATACCGGTATT
GGCATTGCGCCAGAACATCAGCAGAAGTTGTTTCAGTCCTTTTGCCAACTGGACTCTTCCATTACCCGGGAGTATGGCGG
AACGGGATTAGGATTGGCCATTAGCAAGCAACTCTGCGAGCTGATGGGCGGAACCATTTGGGTGGAAAGTCAGGTGGGAA
TCGGCTCAAGCTTTTTCTTTACCATGGCTGCTTCTGCCTTACCCGGCCCTGAAGATCACCGAGCCACGGAGCCCCAATTC
CAAAACAAGCGGGTTTTAATTGTCGAGGACAATGCCAGTACGGGGCAGATTCTCTGCCAACAAGTCCAGGCATGGGGGCT
CGTTCCTACCCTGGCTTCTTCTGGCTTGGAGGCGTTGGCATGGGTCAAGAAAGAGCGGTTTGATGTTGCGATCGCAGATC
TGCAACTCCCCACCATGACCTGGTCTCGACTCCTATCTGAACTAACCCAAAATCATATCCACGCTCCGCTTCCGATTATT
GGTTTATGTGCCATCGGAGAAAGTGCTGCCGTCTGGCAATATCAGCCCCTGCTATCCGCCTCCCTCAGCAAACCGGTTAA
GCAATTTAGGCTCTACCAGACCCTTCTTAAGGCACTCTCCACCCACCAGCCTGATCTTCCCCAGCCTGCCATATCCCAAC
CTCCTCCCAAACCAACGCCCTCCTCCGTCAGAATTTTGGTAGCTGAAGATAATGTTATTAACCAAAAGGTCATTCTTAAA
CTGTTAGACCGCTTAGGTTACCAGGCGGATTTAGTCACCAATGGCTTGGAGGTGCTCACTGCCCTGCAAGAGCAATCCTA
TGACGTGGTGCTAATGGATGTCCAAATGCCGGAGATGGACGGCATCACCACGGCCCAGAGCATTTGTGAACAGTGGGATA
AAACGAATCGGCCTCACATTGTGGCCATGACAGCCAATACAATGCAAGGCGATCGCGAGTCCTATTTAGCTGCAGGTATG
GATGACTACATTGCCAAACCCATTCGTCTTAAAGGGCTAGAACGAATATTGTCAGAATTCACAGAGGCGAAGTGCACCTA
A

Upstream 100 bases:

>100_bases
TGAACACTGTAACAAGGTCAATGCAGGTCTTTGAATGAGCATGCCATAATTACGTCAGCTTTATCCCTCTTTGTTCAAAC
GGCTGAGGGAATCAACGGTT

Downstream 100 bases:

>100_bases
ATAAACCAGATTTCATACCAAAATTTAGAAATTTAGCTTATTAGTCGAGGGGGTTGTAGCGAATCTCAACATCTCGGAAT
ACCTCCAAGGAAATAACTGC

Product: two-component hybrid sensor and regulator histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 826; Mature: 825

Protein sequence:

>826_residues
MPMPHQPADPFDIASPLSLANPFAAFIDQTVDIFVAYDQDLRIITINRIGAQRLQRPYEQILGQTNQQLFGAQAELIDPF
LMSALETGERVFAEQELVFNQQRYYYDTIYTPTTDARTGERQVWGICRDITIKKDLQKKREALLKQQTIQIEERFRTSFN
NATIAKALIALDGSWLQVNPALSQLLGYDPTELLKLRIEDLTHPEDQDIDRHQVRQLLIGQTSSYQIEKRFLHRLGHTLS
VLLSVALILNSQGKPSYFIAEMQDITQRKQAEAALYLQLQKTLQLQTALQTKNQALLESSQAAQAANLAKSEFLAMISHE
IRTPMNAVIGMTDLLKDTHLDPHQQDFVETIRTSGESLLNIINDILDFSKIESGKLELELHPFPISTCVEEALDIVNSKA
TAKGLELAYLIEPGTPNHIQGDANRLRQILVNLLGNAVKFTADGEVIVTISARDVATAEMTPEVPLSLHELLFEVKDTGI
GIAPEHQQKLFQSFCQLDSSITREYGGTGLGLAISKQLCELMGGTIWVESQVGIGSSFFFTMAASALPGPEDHRATEPQF
QNKRVLIVEDNASTGQILCQQVQAWGLVPTLASSGLEALAWVKKERFDVAIADLQLPTMTWSRLLSELTQNHIHAPLPII
GLCAIGESAAVWQYQPLLSASLSKPVKQFRLYQTLLKALSTHQPDLPQPAISQPPPKPTPSSVRILVAEDNVINQKVILK
LLDRLGYQADLVTNGLEVLTALQEQSYDVVLMDVQMPEMDGITTAQSICEQWDKTNRPHIVAMTANTMQGDRESYLAAGM
DDYIAKPIRLKGLERILSEFTEAKCT

Sequences:

>Translated_826_residues
MPMPHQPADPFDIASPLSLANPFAAFIDQTVDIFVAYDQDLRIITINRIGAQRLQRPYEQILGQTNQQLFGAQAELIDPF
LMSALETGERVFAEQELVFNQQRYYYDTIYTPTTDARTGERQVWGICRDITIKKDLQKKREALLKQQTIQIEERFRTSFN
NATIAKALIALDGSWLQVNPALSQLLGYDPTELLKLRIEDLTHPEDQDIDRHQVRQLLIGQTSSYQIEKRFLHRLGHTLS
VLLSVALILNSQGKPSYFIAEMQDITQRKQAEAALYLQLQKTLQLQTALQTKNQALLESSQAAQAANLAKSEFLAMISHE
IRTPMNAVIGMTDLLKDTHLDPHQQDFVETIRTSGESLLNIINDILDFSKIESGKLELELHPFPISTCVEEALDIVNSKA
TAKGLELAYLIEPGTPNHIQGDANRLRQILVNLLGNAVKFTADGEVIVTISARDVATAEMTPEVPLSLHELLFEVKDTGI
GIAPEHQQKLFQSFCQLDSSITREYGGTGLGLAISKQLCELMGGTIWVESQVGIGSSFFFTMAASALPGPEDHRATEPQF
QNKRVLIVEDNASTGQILCQQVQAWGLVPTLASSGLEALAWVKKERFDVAIADLQLPTMTWSRLLSELTQNHIHAPLPII
GLCAIGESAAVWQYQPLLSASLSKPVKQFRLYQTLLKALSTHQPDLPQPAISQPPPKPTPSSVRILVAEDNVINQKVILK
LLDRLGYQADLVTNGLEVLTALQEQSYDVVLMDVQMPEMDGITTAQSICEQWDKTNRPHIVAMTANTMQGDRESYLAAGM
DDYIAKPIRLKGLERILSEFTEAKCT
>Mature_825_residues
PMPHQPADPFDIASPLSLANPFAAFIDQTVDIFVAYDQDLRIITINRIGAQRLQRPYEQILGQTNQQLFGAQAELIDPFL
MSALETGERVFAEQELVFNQQRYYYDTIYTPTTDARTGERQVWGICRDITIKKDLQKKREALLKQQTIQIEERFRTSFNN
ATIAKALIALDGSWLQVNPALSQLLGYDPTELLKLRIEDLTHPEDQDIDRHQVRQLLIGQTSSYQIEKRFLHRLGHTLSV
LLSVALILNSQGKPSYFIAEMQDITQRKQAEAALYLQLQKTLQLQTALQTKNQALLESSQAAQAANLAKSEFLAMISHEI
RTPMNAVIGMTDLLKDTHLDPHQQDFVETIRTSGESLLNIINDILDFSKIESGKLELELHPFPISTCVEEALDIVNSKAT
AKGLELAYLIEPGTPNHIQGDANRLRQILVNLLGNAVKFTADGEVIVTISARDVATAEMTPEVPLSLHELLFEVKDTGIG
IAPEHQQKLFQSFCQLDSSITREYGGTGLGLAISKQLCELMGGTIWVESQVGIGSSFFFTMAASALPGPEDHRATEPQFQ
NKRVLIVEDNASTGQILCQQVQAWGLVPTLASSGLEALAWVKKERFDVAIADLQLPTMTWSRLLSELTQNHIHAPLPIIG
LCAIGESAAVWQYQPLLSASLSKPVKQFRLYQTLLKALSTHQPDLPQPAISQPPPKPTPSSVRILVAEDNVINQKVILKL
LDRLGYQADLVTNGLEVLTALQEQSYDVVLMDVQMPEMDGITTAQSICEQWDKTNRPHIVAMTANTMQGDRESYLAAGMD
DYIAKPIRLKGLERILSEFTEAKCT

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=544, Percent_Identity=34.0073529411765, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI145693157, Length=531, Percent_Identity=32.391713747646, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI48994928, Length=487, Percent_Identity=29.9794661190965, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI87081816, Length=368, Percent_Identity=32.8804347826087, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788713, Length=431, Percent_Identity=31.322505800464, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788381, Length=128, Percent_Identity=39.0625, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=275, Percent_Identity=28.7272727272727, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1788549, Length=388, Percent_Identity=25.2577319587629, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=26.7716535433071, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790436, Length=250, Percent_Identity=26.8, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=26.4840182648402, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=28.2511210762332, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787894, Length=236, Percent_Identity=25.4237288135593, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=271, Percent_Identity=24.7232472324723, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=375, Percent_Identity=29.3333333333333, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322000, Length=117, Percent_Identity=37.6068376068376, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323034, Length=162, Percent_Identity=27.1604938271605, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 92045; Mature: 91913

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMPHQPADPFDIASPLSLANPFAAFIDQTVDIFVAYDQDLRIITINRIGAQRLQRPYEQ
CCCCCCCCCCCCCCCCHHHCCHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHH
ILGQTNQQLFGAQAELIDPFLMSALETGERVFAEQELVFNQQRYYYDTIYTPTTDARTGE
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
RQVWGICRDITIKKDLQKKREALLKQQTIQIEERFRTSFNNATIAKALIALDGSWLQVNP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECH
ALSQLLGYDPTELLKLRIEDLTHPEDQDIDRHQVRQLLIGQTSSYQIEKRFLHRLGHTLS
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VLLSVALILNSQGKPSYFIAEMQDITQRKQAEAALYLQLQKTLQLQTALQTKNQALLESS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAQAANLAKSEFLAMISHEIRTPMNAVIGMTDLLKDTHLDPHQQDFVETIRTSGESLLN
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHH
IINDILDFSKIESGKLELELHPFPISTCVEEALDIVNSKATAKGLELAYLIEPGTPNHIQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCCCCCC
GDANRLRQILVNLLGNAVKFTADGEVIVTISARDVATAEMTPEVPLSLHELLFEVKDTGI
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GIAPEHQQKLFQSFCQLDSSITREYGGTGLGLAISKQLCELMGGTIWVESQVGIGSSFFF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
TMAASALPGPEDHRATEPQFQNKRVLIVEDNASTGQILCQQVQAWGLVPTLASSGLEALA
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCHHHHH
WVKKERFDVAIADLQLPTMTWSRLLSELTQNHIHAPLPIIGLCAIGESAAVWQYQPLLSA
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCCCEEEHHHHHH
SLSKPVKQFRLYQTLLKALSTHQPDLPQPAISQPPPKPTPSSVRILVAEDNVINQKVILK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LLDRLGYQADLVTNGLEVLTALQEQSYDVVLMDVQMPEMDGITTAQSICEQWDKTNRPHI
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEE
VAMTANTMQGDRESYLAAGMDDYIAKPIRLKGLERILSEFTEAKCT
EEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMPHQPADPFDIASPLSLANPFAAFIDQTVDIFVAYDQDLRIITINRIGAQRLQRPYEQ
CCCCCCCCCCCCCCCHHHCCHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHH
ILGQTNQQLFGAQAELIDPFLMSALETGERVFAEQELVFNQQRYYYDTIYTPTTDARTGE
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
RQVWGICRDITIKKDLQKKREALLKQQTIQIEERFRTSFNNATIAKALIALDGSWLQVNP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECH
ALSQLLGYDPTELLKLRIEDLTHPEDQDIDRHQVRQLLIGQTSSYQIEKRFLHRLGHTLS
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VLLSVALILNSQGKPSYFIAEMQDITQRKQAEAALYLQLQKTLQLQTALQTKNQALLESS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAQAANLAKSEFLAMISHEIRTPMNAVIGMTDLLKDTHLDPHQQDFVETIRTSGESLLN
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHH
IINDILDFSKIESGKLELELHPFPISTCVEEALDIVNSKATAKGLELAYLIEPGTPNHIQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCCCCCC
GDANRLRQILVNLLGNAVKFTADGEVIVTISARDVATAEMTPEVPLSLHELLFEVKDTGI
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GIAPEHQQKLFQSFCQLDSSITREYGGTGLGLAISKQLCELMGGTIWVESQVGIGSSFFF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
TMAASALPGPEDHRATEPQFQNKRVLIVEDNASTGQILCQQVQAWGLVPTLASSGLEALA
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCHHHHH
WVKKERFDVAIADLQLPTMTWSRLLSELTQNHIHAPLPIIGLCAIGESAAVWQYQPLLSA
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCCCEEEHHHHHH
SLSKPVKQFRLYQTLLKALSTHQPDLPQPAISQPPPKPTPSSVRILVAEDNVINQKVILK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LLDRLGYQADLVTNGLEVLTALQEQSYDVVLMDVQMPEMDGITTAQSICEQWDKTNRPHI
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEE
VAMTANTMQGDRESYLAAGMDDYIAKPIRLKGLERILSEFTEAKCT
EEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]