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The map label for this gene is carB [C]

Identifier: 158338766

GI number: 158338766

Start: 5741222

End: 5742418

Strand: Direct

Name: carB [C]

Synonym: AM1_5677

Alternate gene names: 158338766

Gene position: 5741222-5742418 (Clockwise)

Preceding gene: 158338765

Following gene: 158338767

Centisome position: 88.28

GC content: 29.74

Gene sequence:

>1197_bases
TTGAAGGAGAAAGACTATAAAATAACGTATGTCATTTTTAAGTCTAAAAAAATAAAATATATGAAAAATAAATATCATTC
AATTTTAATAATAGATGAATGTGATCAGCCCATTGCTTATTATGTATTGAGATGTTTGAAATCGGGGAATCAGAATTTAA
AGATCAGTATAATTATCCCGTTAAACCAGTCATCTATAGAAAATAATTGGATAACTTTCTATAAATATTCGAGATATATA
GATCGTCTAATTATTGCAAGTAATGAGATAGATTCCAATGACTTTCTGAATGAAGTAATTCAAGCAATCCAGCAAGAATC
GATCGATTATATTTTTCCGGCTTCTGAAAATAGTTTTAAGTTTGTATCCAGACATAGGGTTCAACTATCATCTTCAAGTA
ACTTAATAGCCTTACCCAGCCAAAATTCTTTAGATGTGACATTTAATAAGTGGGAATTGAGTCTTTTTCTGAAAAACAGA
AAAATTCCAACTCCTAAAACTATTCTACCAAGTTCAATCACTCAATCGAATCAATTATCTTACCCAATTCTACTAAAACC
AGCCTGCGGAAGTGGAGGTGAAAATATTAAAAAGATCGAAGAAGTATCAGTGCTTAAATCTATAATACATAATCCAATTG
CTAAGAATGATTATAAAGATTATATATTGCAAGAATATATTGATGGCTATGATATTGATTGTAATGTACTCTGCAAAGAT
GGAAAGATTCTTACTCATTCTGTGCAAAAACCATTAGGTATAGAAAAAGGATTTTCGCCAAAGATTGATAAATTACATTT
TATTCATGATAACTCTGTATTGAGTATCGTAAAAAAAACAATGTTTGAGCTTCAATGGAGTGGAATTGCCCATCTAGATC
TTAGGTACTGCCATAAAACAGGTAATCTATACTTAATTGAAATAAACCCTAGATTTTGGCAATCTTTAATGGGCTCACTT
TCCATTGGTATTAATTTCCCATATCTACTATTTCTAGTATCAGCTGGGTTTGATATCGGAGACGTTTATTATGATGATCA
ATACTATGCAAAGTTTTCTAGATATATAAAAGATATACTTAGTGGTTCACTAGACTATAGTCTAGATAATACTAACCTAA
AATACTTGCTGACAGATTTAAATTCAATTGCTAATTATGGTATAAAACGTTTTTTTGGAAGCAAGACCATTAGTTAG

Upstream 100 bases:

>100_bases
GAATTACTAGTATTGTTAGAGGATGGTTTTAAGCTATTTGGAATAATAAGTTGGCTTACATATTTAAGTAAAACTTCCTT
GTTGATTATAGAAACCATTA

Downstream 100 bases:

>100_bases
TTTAGCTATTTTATTGATATATCTTATAAAGAGTAAAAATGGACGTAAAGATAATTAATACAGAGAATGATTTGTGGCTA
AAAGTCTTATCGAACTTGCA

Product: hypothetical protein

Products: ADP; Carbamoyl phosphate; L-Glutamate; H+; Phosphate [C]

Alternate protein names: ATP-Grasp -Like Protein; Carbamoylphosphate Synthase; ATP-Grasp Protein; ATP-Grasp -Like; ATP-Grasp Protein-Like Protein; ATP-Grasp Domain Protein

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKEKDYKITYVIFKSKKIKYMKNKYHSILIIDECDQPIAYYVLRCLKSGNQNLKISIIIPLNQSSIENNWITFYKYSRYI
DRLIIASNEIDSNDFLNEVIQAIQQESIDYIFPASENSFKFVSRHRVQLSSSSNLIALPSQNSLDVTFNKWELSLFLKNR
KIPTPKTILPSSITQSNQLSYPILLKPACGSGGENIKKIEEVSVLKSIIHNPIAKNDYKDYILQEYIDGYDIDCNVLCKD
GKILTHSVQKPLGIEKGFSPKIDKLHFIHDNSVLSIVKKTMFELQWSGIAHLDLRYCHKTGNLYLIEINPRFWQSLMGSL
SIGINFPYLLFLVSAGFDIGDVYYDDQYYAKFSRYIKDILSGSLDYSLDNTNLKYLLTDLNSIANYGIKRFFGSKTIS

Sequences:

>Translated_398_residues
MKEKDYKITYVIFKSKKIKYMKNKYHSILIIDECDQPIAYYVLRCLKSGNQNLKISIIIPLNQSSIENNWITFYKYSRYI
DRLIIASNEIDSNDFLNEVIQAIQQESIDYIFPASENSFKFVSRHRVQLSSSSNLIALPSQNSLDVTFNKWELSLFLKNR
KIPTPKTILPSSITQSNQLSYPILLKPACGSGGENIKKIEEVSVLKSIIHNPIAKNDYKDYILQEYIDGYDIDCNVLCKD
GKILTHSVQKPLGIEKGFSPKIDKLHFIHDNSVLSIVKKTMFELQWSGIAHLDLRYCHKTGNLYLIEINPRFWQSLMGSL
SIGINFPYLLFLVSAGFDIGDVYYDDQYYAKFSRYIKDILSGSLDYSLDNTNLKYLLTDLNSIANYGIKRFFGSKTIS
>Mature_398_residues
MKEKDYKITYVIFKSKKIKYMKNKYHSILIIDECDQPIAYYVLRCLKSGNQNLKISIIIPLNQSSIENNWITFYKYSRYI
DRLIIASNEIDSNDFLNEVIQAIQQESIDYIFPASENSFKFVSRHRVQLSSSSNLIALPSQNSLDVTFNKWELSLFLKNR
KIPTPKTILPSSITQSNQLSYPILLKPACGSGGENIKKIEEVSVLKSIIHNPIAKNDYKDYILQEYIDGYDIDCNVLCKD
GKILTHSVQKPLGIEKGFSPKIDKLHFIHDNSVLSIVKKTMFELQWSGIAHLDLRYCHKTGNLYLIEINPRFWQSLMGSL
SIGINFPYLLFLVSAGFDIGDVYYDDQYYAKFSRYIKDILSGSLDYSLDNTNLKYLLTDLNSIANYGIKRFFGSKTIS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.5 [C]

Molecular weight: Translated: 45884; Mature: 45884

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50975 ATP_GRASP ; PS00334 MYB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKDYKITYVIFKSKKIKYMKNKYHSILIIDECDQPIAYYVLRCLKSGNQNLKISIIIP
CCCCCEEEEEEEEECCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEE
LNQSSIENNWITFYKYSRYIDRLIIASNEIDSNDFLNEVIQAIQQESIDYIFPASENSFK
CCCCCCCCCCEEEEEHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHH
FVSRHRVQLSSSSNLIALPSQNSLDVTFNKWELSLFLKNRKIPTPKTILPSSITQSNQLS
HHHHEEEEECCCCCEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
YPILLKPACGSGGENIKKIEEVSVLKSIIHNPIAKNDYKDYILQEYIDGYDIDCNVLCKD
CCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
GKILTHSVQKPLGIEKGFSPKIDKLHFIHDNSVLSIVKKTMFELQWSGIAHLDLRYCHKT
CEEEEHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEECC
GNLYLIEINPRFWQSLMGSLSIGINFPYLLFLVSAGFDIGDVYYDDQYYAKFSRYIKDIL
CCEEEEEECHHHHHHHHCCEEECCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHH
SGSLDYSLDNTNLKYLLTDLNSIANYGIKRFFGSKTIS
CCCCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKEKDYKITYVIFKSKKIKYMKNKYHSILIIDECDQPIAYYVLRCLKSGNQNLKISIIIP
CCCCCEEEEEEEEECCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEE
LNQSSIENNWITFYKYSRYIDRLIIASNEIDSNDFLNEVIQAIQQESIDYIFPASENSFK
CCCCCCCCCCEEEEEHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHH
FVSRHRVQLSSSSNLIALPSQNSLDVTFNKWELSLFLKNRKIPTPKTILPSSITQSNQLS
HHHHEEEEECCCCCEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
YPILLKPACGSGGENIKKIEEVSVLKSIIHNPIAKNDYKDYILQEYIDGYDIDCNVLCKD
CCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
GKILTHSVQKPLGIEKGFSPKIDKLHFIHDNSVLSIVKKTMFELQWSGIAHLDLRYCHKT
CEEEEHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEECC
GNLYLIEINPRFWQSLMGSLSIGINFPYLLFLVSAGFDIGDVYYDDQYYAKFSRYIKDIL
CCEEEEEECHHHHHHHHCCEEECCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHH
SGSLDYSLDNTNLKYLLTDLNSIANYGIKRFFGSKTIS
CCCCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; GDP; GMP; GTP; IDP; IMP; ITP; N-Acetylglutamate; NEM; Orn; Thiol; XMP [C]

Metal ions: K+; Mg2+; Mn2+; NH4+ [C]

Kcat value (1/min): 120000 [C]

Specific activity: NA

Km value (mM): 93 {NH4+}} 0.38 {L-Gln}} [C]

Substrates: ATP; L-Glutamine; H2O; Bicarbonate [C]

Specific reaction: (2) ATP + L-Glutamine + H2O + Bicarbonate --> (2) ADP + Carbamoyl phosphate + L-Glutamate + (2) H+ + Phosphate [C]

General reaction: Amide group transfer; Amination; Phosphorylation [C]

Inhibitor: 6-Diazo-5-oxon/leucine; Alkyl hydrazines; Azaserine; Guanidine; H2O2; NH2OH; L-2-Amino-4-oxo-5-chloropentanoate; L-Cyanate; NEM; P1, P5-Di (adenosine 5')-pentaphosphate; UDP; UMP phosphate; Urea; UTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA