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The map label for this gene is radA [H]

Identifier: 158338751

GI number: 158338751

Start: 5725710

End: 5727182

Strand: Reverse

Name: radA [H]

Synonym: AM1_5662

Alternate gene names: 158338751

Gene position: 5727182-5725710 (Counterclockwise)

Preceding gene: 158338753

Following gene: 158338750

Centisome position: 88.06

GC content: 50.58

Gene sequence:

>1473_bases
ATGGCAAAAATACGAACTCATTACATTTGCCAAGAATGTGGTGCAGCTTCTCCTCAGTTTTTTGGTAAATGTCCCTCCTG
TCACACTTGGAATTCTTTGGTCGAACAAGTGGTGCCAAATACACCGGCGGCAACCGACGGAACCCGCTTGGCACAACGAC
ATCGCCAACGTCAAAACACTTCACCCAAGGCACTTTCCTCCCTGACTTTGGCCCAAATCTCTGAGCAGCCAATTCAGCGG
TTTAGCTCCGGTTATAGTGAACTCGATCGGGTGTTAGGAGGCGGTATTGTTCCCGGTTCCCTGGTGTTGGTGGGCGGTGA
TCCGGGAATTGGTAAATCGACTCTTTTGTTGCAAATTGTCAACTTTTTAAGTGCCCGACAAACGGTTCTCTATGTTTGTG
CAGAAGAATCTGGCCAACAGGTTAAATTGCGTGCCCAGCGATTGGGAATTGGAACCTTAAATTTAAATAAAGAAATCACG
GATCCACCTCCCAAATCAAAAGACAAATCCGCAAATGCCGATTTATACCTGTTGCCGGAAACGGATTTAGAAATGGTGTT
GCAAGAACTAGATTCCCTGCAACCGCAAGTGGCCATTATTGATAGTATTCAAGCCCTATTTTTCTCCACTTTAACGTCGG
CTCCTGGCTCTGTTGCCCAGGTGAGGGAATGCACATCAGCCCTGATGCGCTTGGCGAAACGGAACCACATTACATTGTTT
ATCGTTGGCCATGTCACGAAGGAAGGTGCCATCGCCGGTCCCAAGGTTTTAGAACATTTAGTGGATACGGTGCTCTACTT
TGAAGGGGATCGATTTGCCAATCACCGATTACTCCGATCCGTCAAAAATCGGTTTGGCGCAACTCACGAAGTCGGTGTAT
TTGAAATGATTGATCGGGGCTTGCGGGAAGTCTTGAATCCATCGGAGTTATTTTTGGGCGATCGTGAAGAGGTATCGCCC
GGAACAGCCACTATCGTGGCTTGTGAAGGCACCCGCCCCATTGTGGTGGAACTACAGGCCTTGGTTAGCCCCACCAGCTA
CAGTTCACCTCGCCGCTCAACGACAGGGATTGAATATAATCGGTTGCTGCAAATTCTGGCGGTGTTAGAGAAACGGCTGG
GCATTCCTCTGTCTAAGCTGGATGCCTATGTGGCCTCTTCCGGCGGATTGAGTGTGGCTGAACCTGCGGCAGATTTGGGA
GTTGCGATCGCACTGGCAGCCAGCTTCCGTGATCGCCTGGTAGACCCTACCACCGTTGTGATTGGTGAAGTGGGATTGGG
GGGGCAAGTTCGCTCCGTATCGCAAACCGAACTACGGCTCAAAGAAGCCCTGAAACTAGGATTTAAACGGGCCATCATTC
CCAAAGGCCAGTCTTTTCCCAGTCTGGATATAGAAGTCATCCCCGTCGCCCGAGTTGCCGACGCCATTGTGGCCGCCCTG
CCGGGTGGATCATCGGCTCATTCGACAGAGTGA

Upstream 100 bases:

>100_bases
TATTAAGTTAGCATGAAGTTAGTAACAGGAAAAAACGCTGAAATGCTTTCAGACTAAAGACTTTAAAAATATTTACAGTT
CAATTTTATTTAAGAATTAT

Downstream 100 bases:

>100_bases
AGTCTTTGGATATCGCTTGTGAAAGAAAAGGTATACATTCCGCCCCTTCTTTACACCTATTTATTTCCTAGGTGCTCTGA
ATGATACCCCTACTAATCAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAKIRTHYICQECGAASPQFFGKCPSCHTWNSLVEQVVPNTPAATDGTRLAQRHRQRQNTSPKALSSLTLAQISEQPIQR
FSSGYSELDRVLGGGIVPGSLVLVGGDPGIGKSTLLLQIVNFLSARQTVLYVCAEESGQQVKLRAQRLGIGTLNLNKEIT
DPPPKSKDKSANADLYLLPETDLEMVLQELDSLQPQVAIIDSIQALFFSTLTSAPGSVAQVRECTSALMRLAKRNHITLF
IVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFANHRLLRSVKNRFGATHEVGVFEMIDRGLREVLNPSELFLGDREEVSP
GTATIVACEGTRPIVVELQALVSPTSYSSPRRSTTGIEYNRLLQILAVLEKRLGIPLSKLDAYVASSGGLSVAEPAADLG
VAIALAASFRDRLVDPTTVVIGEVGLGGQVRSVSQTELRLKEALKLGFKRAIIPKGQSFPSLDIEVIPVARVADAIVAAL
PGGSSAHSTE

Sequences:

>Translated_490_residues
MAKIRTHYICQECGAASPQFFGKCPSCHTWNSLVEQVVPNTPAATDGTRLAQRHRQRQNTSPKALSSLTLAQISEQPIQR
FSSGYSELDRVLGGGIVPGSLVLVGGDPGIGKSTLLLQIVNFLSARQTVLYVCAEESGQQVKLRAQRLGIGTLNLNKEIT
DPPPKSKDKSANADLYLLPETDLEMVLQELDSLQPQVAIIDSIQALFFSTLTSAPGSVAQVRECTSALMRLAKRNHITLF
IVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFANHRLLRSVKNRFGATHEVGVFEMIDRGLREVLNPSELFLGDREEVSP
GTATIVACEGTRPIVVELQALVSPTSYSSPRRSTTGIEYNRLLQILAVLEKRLGIPLSKLDAYVASSGGLSVAEPAADLG
VAIALAASFRDRLVDPTTVVIGEVGLGGQVRSVSQTELRLKEALKLGFKRAIIPKGQSFPSLDIEVIPVARVADAIVAAL
PGGSSAHSTE
>Mature_489_residues
AKIRTHYICQECGAASPQFFGKCPSCHTWNSLVEQVVPNTPAATDGTRLAQRHRQRQNTSPKALSSLTLAQISEQPIQRF
SSGYSELDRVLGGGIVPGSLVLVGGDPGIGKSTLLLQIVNFLSARQTVLYVCAEESGQQVKLRAQRLGIGTLNLNKEITD
PPPKSKDKSANADLYLLPETDLEMVLQELDSLQPQVAIIDSIQALFFSTLTSAPGSVAQVRECTSALMRLAKRNHITLFI
VGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFANHRLLRSVKNRFGATHEVGVFEMIDRGLREVLNPSELFLGDREEVSPG
TATIVACEGTRPIVVELQALVSPTSYSSPRRSTTGIEYNRLLQILAVLEKRLGIPLSKLDAYVASSGGLSVAEPAADLGV
AIALAASFRDRLVDPTTVVIGEVGLGGQVRSVSQTELRLKEALKLGFKRAIIPKGQSFPSLDIEVIPVARVADAIVAALP
GGSSAHSTE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=480, Percent_Identity=42.5, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 52652; Mature: 52521

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIRTHYICQECGAASPQFFGKCPSCHTWNSLVEQVVPNTPAATDGTRLAQRHRQRQNT
CCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
SPKALSSLTLAQISEQPIQRFSSGYSELDRVLGGGIVPGSLVLVGGDPGIGKSTLLLQIV
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHH
NFLSARQTVLYVCAEESGQQVKLRAQRLGIGTLNLNKEITDPPPKSKDKSANADLYLLPE
HHHHHCCEEEEEEECCCCCEEEEEHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECC
TDLEMVLQELDSLQPQVAIIDSIQALFFSTLTSAPGSVAQVRECTSALMRLAKRNHITLF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEE
IVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFANHRLLRSVKNRFGATHEVGVFEMIDRG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LREVLNPSELFLGDREEVSPGTATIVACEGTRPIVVELQALVSPTSYSSPRRSTTGIEYN
HHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHH
RLLQILAVLEKRLGIPLSKLDAYVASSGGLSVAEPAADLGVAIALAASFRDRLVDPTTVV
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
IGEVGLGGQVRSVSQTELRLKEALKLGFKRAIIPKGQSFPSLDIEVIPVARVADAIVAAL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHC
PGGSSAHSTE
CCCCCCCCCC
>Mature Secondary Structure 
AKIRTHYICQECGAASPQFFGKCPSCHTWNSLVEQVVPNTPAATDGTRLAQRHRQRQNT
CCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
SPKALSSLTLAQISEQPIQRFSSGYSELDRVLGGGIVPGSLVLVGGDPGIGKSTLLLQIV
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHH
NFLSARQTVLYVCAEESGQQVKLRAQRLGIGTLNLNKEITDPPPKSKDKSANADLYLLPE
HHHHHCCEEEEEEECCCCCEEEEEHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECC
TDLEMVLQELDSLQPQVAIIDSIQALFFSTLTSAPGSVAQVRECTSALMRLAKRNHITLF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEE
IVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFANHRLLRSVKNRFGATHEVGVFEMIDRG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LREVLNPSELFLGDREEVSPGTATIVACEGTRPIVVELQALVSPTSYSSPRRSTTGIEYN
HHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHH
RLLQILAVLEKRLGIPLSKLDAYVASSGGLSVAEPAADLGVAIALAASFRDRLVDPTTVV
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
IGEVGLGGQVRSVSQTELRLKEALKLGFKRAIIPKGQSFPSLDIEVIPVARVADAIVAAL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHC
PGGSSAHSTE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]