Click here to switch to the map view.

The map label for this gene is pyrC

Identifier: 158338736

GI number: 158338736

Start: 5708680

End: 5709717

Strand: Reverse

Name: pyrC

Synonym: AM1_5644

Alternate gene names: 158338736

Gene position: 5709717-5708680 (Counterclockwise)

Preceding gene: 158338747

Following gene: 158338733

Centisome position: 87.79

GC content: 50.48

Gene sequence:

>1038_bases
ATGCAAAAACTCACCCTCACTCGGCCCGACGATTGGCACCTCCATCTACGGGATGGAGATGCCTTAAAGGCAGTTCTCCC
CCACTCGGCACGCCAGTTCGCTCGGGCCATTGTTATGCCCAATCTAAAGCCCCCAGTCCGTACCGTGGCTGATGCTGCAG
ACTATCGGAACCGAATTTTGGCAGCCCTTCCAGTGGGTCAAACCTTTGAACCTTTGATGACCCTCTATCTGACCGACAAT
ACTAGCCCTGAAGAAATCAGTGCTGCCAAAGCATCGAAATTTGTCAAGGCGGTGAAATACTATCCAGCAGGGGCAACTAC
CAATTCTGACTTTGGTGTGACGGATATTCGGAAATGCGATCGCATCTTCGCAGCCATGGAGCAAGAAGATATCCCGCTGT
TACTCCATGGAGAAGTGACCGACACCAATATTGATGTGTTTGATCGCGAAAAAGTGTTTATCGAAACCCACCTGATTCCC
CTCGTATCGCGATTTCCTAAACTGCGAGTGGTACTTGAACATATCACCACCTCCGAAGCAGTGACGTTTGTCCTCAATAC
CAACGAAAACATTGGTGCAACGATCACGCCTCAACACCTTCTATTCAGTCGTAATGCCATATTTAAAGGAGGAATTCGTC
CCCATTATTATTGCCTGCCGATTCTGAAACGAGAGAAACATCGGCAGGCGCTGTTGCAGGCAGCGACATCCGGCAATCCC
AAGTTTTTTCTCGGCACCGATAGCGCCCCCCATGCCCGCAATTCGAAAGAGCAGTCCTGTGGCTGCGCTGGTTGCTATTC
GGCCCTCCATGCCATGGAATTATATGCGGAAGCATTTGAAAGTGCGAATGCCCTCGACAAACTGGAAGCCTTTGCCAGCT
TCTATGGTCCAGACTTTTACCAACTTCCCCGCAATACCAAGACAATCACTTTGGCGAAACAGACTTGGCAAATCCCTGAT
GAAGTCCCCTTCCCAGGAACTGGCCTTGTCCCCCTCAGGGCAGGAGAAGAAATCACCTGGAAGATAATGGACGGATAG

Upstream 100 bases:

>100_bases
GATTTATTCAGGTAAGTTCAGGTGTTTTCTGCTTGTCTGCTCCCGTGACTAAAGCAAAAGGATGTGGAGTAAGCTAACGA
CAAGAATTTATGATTGAGAG

Downstream 100 bases:

>100_bases
TATTGCCCATCAGCAGACCAAATCACAGCCAGTTACCTACCAGCACAAATGGACTCCAAAAATAGGGGGCTTTAAATTGT
GGGTCCTTCATCAAAGCCAA

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MQKLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRILAALPVGQTFEPLMTLYLTDN
TSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDIRKCDRIFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIP
LVSRFPKLRVVLEHITTSEAVTFVLNTNENIGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP
KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFYQLPRNTKTITLAKQTWQIPD
EVPFPGTGLVPLRAGEEITWKIMDG

Sequences:

>Translated_345_residues
MQKLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRILAALPVGQTFEPLMTLYLTDN
TSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDIRKCDRIFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIP
LVSRFPKLRVVLEHITTSEAVTFVLNTNENIGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP
KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFYQLPRNTKTITLAKQTWQIPD
EVPFPGTGLVPLRAGEEITWKIMDG
>Mature_345_residues
MQKLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRILAALPVGQTFEPLMTLYLTDN
TSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDIRKCDRIFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIP
LVSRFPKLRVVLEHITTSEAVTFVLNTNENIGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP
KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFYQLPRNTKTITLAKQTWQIPD
EVPFPGTGLVPLRAGEEITWKIMDG

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=341, Percent_Identity=52.7859237536657, Blast_Score=370, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323452, Length=367, Percent_Identity=34.0599455040872, Blast_Score=183, Evalue=4e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_ACAM1 (B0CF78)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519913.1
- ProteinModelPortal:   B0CF78
- SMR:   B0CF78
- GeneID:   5684434
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5644
- HOGENOM:   HBG628648
- OMA:   KESACGC
- ProtClustDB:   PRK05451
- BioCyc:   AMAR329726:AM1_5644-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 38498; Mature: 38498

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRIL
CCCCEECCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEE
AALPVGQTFEPLMTLYLTDNTSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDIRKCD
EEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RIFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIPLVSRFPKLRVVLEHITTSEA
HHHHHHCCCCCCEEEECCEECCCCCEECCCEEEEEHHHHHHHHHCHHHHHHHHHHCCCCE
VTFVLNTNENIGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP
EEEEEECCCCCCCEECHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCC
KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFY
EEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCE
QLPRNTKTITLAKQTWQIPDEVPFPGTGLVPLRAGEEITWKIMDG
ECCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEECC
>Mature Secondary Structure
MQKLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRIL
CCCCEECCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEE
AALPVGQTFEPLMTLYLTDNTSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDIRKCD
EEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RIFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIPLVSRFPKLRVVLEHITTSEA
HHHHHHCCCCCCEEEECCEECCCCCEECCCEEEEEHHHHHHHHHCHHHHHHHHHHCCCCE
VTFVLNTNENIGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP
EEEEEECCCCCCCEECHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCC
KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFY
EEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCE
QLPRNTKTITLAKQTWQIPDEVPFPGTGLVPLRAGEEITWKIMDG
ECCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA