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The map label for this gene is pleD [H]

Identifier: 158338729

GI number: 158338729

Start: 5697101

End: 5698102

Strand: Reverse

Name: pleD [H]

Synonym: AM1_5637

Alternate gene names: 158338729

Gene position: 5698102-5697101 (Counterclockwise)

Preceding gene: 158338730

Following gene: 158338722

Centisome position: 87.61

GC content: 46.81

Gene sequence:

>1002_bases
ATGCGCAACACGACTCAAACCAGCAGTGCGCATAACCCATCCTCTAACGCCTCTATCGAGGCACCTCCACCCCCTGAACC
CACTGTCCCCACTGGAAAAGTCAAAATCTTACTGGTGGATGACCAACCCCAAAATCTACGGTTGCTCACAGACGTATTAG
AAGAACAGGGCTATGAAGTTCTCCAAGCTCTCAACGGCAATGTTGCTCTCCAGGCCGTGTCTCTCGATCAACCTCAGTTG
ATTCTCTTAGATATTCAAATGCCAGAAATGGATGGTTACACTGTTTGTCAAAAGCTTAAAGCTGATCCATCCACCCAAGA
CATTCCCGTAGTCTTCGTTAGCGCTCTGGATGAATCTTGGGATAAGGTCAAAGCCTTTTCTGTGGGAGGAGTAGATTACA
TCACCAAACCCTTTAAGGTCGTAGAGGTCTTAGCCCGAGTACAAAATCAACTAAAAATTCAACAACTCCAGCAAGCGCTT
AAGACTCAAAACGCACAACTCCACCAAGCAAATTTAGAACTGCAGCGTCTAGCTGCCCTGGATGATGTCACCCAGGTCGC
CAACCGCCGCCGCTTTGACGCCTACCTTTTGACATCCTGGCATGAAGCCATTCAAGACCAACACTCTTTGACGTTAATGC
TCTGCCAACTTGATCATTTCAATTTCTATGGTTCAGGTGGAAATTCCCAAAAAGGAGACCAGTATTTGTATCGCATTGCT
CAAACTATCAAGCAAACTGTACATGGTCCGAATGATCTAGTTTGTCGATATGGCACCTTCACCTTTGCCATCGTTTTACC
CCAACAGACTTCTGCCCAAGCTGAGGCAATTGCTCAAGAGTTATCAAAGCAAGTCAAGGAAGGAGAGGAGACCTTTTCTT
TTAGTATTGGCCTCGCCTCCATCCAGCCCACATCAGATATAGGCATTGAGGCATTCATAGAAGCCGGTGATCGCAACCTA
CAGCAAGCAAGGAACAATGGCGGCAGTTGCGTATTCACTTAA

Upstream 100 bases:

>100_bases
TGTCAAACAGGATGTTGCCCCTAAACTCTGGCATTTGCTGTCCCAAGCCCTAGATAAAAAAGTTTCAAAATCTAATGTGC
GAATCATCCTCACTCAGGAG

Downstream 100 bases:

>100_bases
GTCTTGCTTGCAATATCAACGGCGCTGCAGTTTTAAGACATCTATAATTAATTCTTGGGCACGAATCTTAGAATTTGAAT
TGGATTTTCTCCGATATCTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRNTTQTSSAHNPSSNASIEAPPPPEPTVPTGKVKILLVDDQPQNLRLLTDVLEEQGYEVLQALNGNVALQAVSLDQPQL
ILLDIQMPEMDGYTVCQKLKADPSTQDIPVVFVSALDESWDKVKAFSVGGVDYITKPFKVVEVLARVQNQLKIQQLQQAL
KTQNAQLHQANLELQRLAALDDVTQVANRRRFDAYLLTSWHEAIQDQHSLTLMLCQLDHFNFYGSGGNSQKGDQYLYRIA
QTIKQTVHGPNDLVCRYGTFTFAIVLPQQTSAQAEAIAQELSKQVKEGEETFSFSIGLASIQPTSDIGIEAFIEAGDRNL
QQARNNGGSCVFT

Sequences:

>Translated_333_residues
MRNTTQTSSAHNPSSNASIEAPPPPEPTVPTGKVKILLVDDQPQNLRLLTDVLEEQGYEVLQALNGNVALQAVSLDQPQL
ILLDIQMPEMDGYTVCQKLKADPSTQDIPVVFVSALDESWDKVKAFSVGGVDYITKPFKVVEVLARVQNQLKIQQLQQAL
KTQNAQLHQANLELQRLAALDDVTQVANRRRFDAYLLTSWHEAIQDQHSLTLMLCQLDHFNFYGSGGNSQKGDQYLYRIA
QTIKQTVHGPNDLVCRYGTFTFAIVLPQQTSAQAEAIAQELSKQVKEGEETFSFSIGLASIQPTSDIGIEAFIEAGDRNL
QQARNNGGSCVFT
>Mature_333_residues
MRNTTQTSSAHNPSSNASIEAPPPPEPTVPTGKVKILLVDDQPQNLRLLTDVLEEQGYEVLQALNGNVALQAVSLDQPQL
ILLDIQMPEMDGYTVCQKLKADPSTQDIPVVFVSALDESWDKVKAFSVGGVDYITKPFKVVEVLARVQNQLKIQQLQQAL
KTQNAQLHQANLELQRLAALDDVTQVANRRRFDAYLLTSWHEAIQDQHSLTLMLCQLDHFNFYGSGGNSQKGDQYLYRIA
QTIKQTVHGPNDLVCRYGTFTFAIVLPQQTSAQAEAIAQELSKQVKEGEETFSFSIGLASIQPTSDIGIEAFIEAGDRNL
QQARNNGGSCVFT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=132, Percent_Identity=36.3636363636364, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788550, Length=136, Percent_Identity=33.8235294117647, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=34.7457627118644, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082012, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=31.9327731092437, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=32.4786324786325, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=30.1724137931034, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=106, Percent_Identity=34.9056603773585, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=30.5084745762712, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=153, Percent_Identity=31.3725490196078, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790437, Length=106, Percent_Identity=34.9056603773585, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36797; Mature: 36797

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTTQTSSAHNPSSNASIEAPPPPEPTVPTGKVKILLVDDQPQNLRLLTDVLEEQGYEV
CCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LQALNGNVALQAVSLDQPQLILLDIQMPEMDGYTVCQKLKADPSTQDIPVVFVSALDESW
HHHCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHCCH
DKVKAFSVGGVDYITKPFKVVEVLARVQNQLKIQQLQQALKTQNAQLHQANLELQRLAAL
HHHEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDVTQVANRRRFDAYLLTSWHEAIQDQHSLTLMLCQLDHFNFYGSGGNSQKGDQYLYRIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHH
QTIKQTVHGPNDLVCRYGTFTFAIVLPQQTSAQAEAIAQELSKQVKEGEETFSFSIGLAS
HHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEE
IQPTSDIGIEAFIEAGDRNLQQARNNGGSCVFT
CCCCCCCCHHHHHHHCCHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MRNTTQTSSAHNPSSNASIEAPPPPEPTVPTGKVKILLVDDQPQNLRLLTDVLEEQGYEV
CCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LQALNGNVALQAVSLDQPQLILLDIQMPEMDGYTVCQKLKADPSTQDIPVVFVSALDESW
HHHCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHCCH
DKVKAFSVGGVDYITKPFKVVEVLARVQNQLKIQQLQQALKTQNAQLHQANLELQRLAAL
HHHEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDVTQVANRRRFDAYLLTSWHEAIQDQHSLTLMLCQLDHFNFYGSGGNSQKGDQYLYRIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHH
QTIKQTVHGPNDLVCRYGTFTFAIVLPQQTSAQAEAIAQELSKQVKEGEETFSFSIGLAS
HHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEE
IQPTSDIGIEAFIEAGDRNLQQARNNGGSCVFT
CCCCCCCCHHHHHHHCCHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA