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The map label for this gene is folE [H]

Identifier: 158338727

GI number: 158338727

Start: 5695825

End: 5696523

Strand: Direct

Name: folE [H]

Synonym: AM1_5635

Alternate gene names: 158338727

Gene position: 5695825-5696523 (Clockwise)

Preceding gene: 158338726

Following gene: 158338728

Centisome position: 87.58

GC content: 47.21

Gene sequence:

>699_bases
ATGACTACTTTTTCTCCTAAACCTTCTGATCAAGCCAATTCTTCTATGAACTCAACTTCTAATAACGCTCAGCTTCGTCC
CGATCGCAGCTTATCTCAACGGGCCAAGGAGAAAGAGACCCATCGTCAAGCCAGCAAAGAAGAGATGATGGGAGCGGTAG
AAACGATGCTGCTAGGGGTTGGTGAAGATCCAGAGCGGGAAGGTTTACTGAAAACACCGAAGCGGGTGGCTGAAGCCATG
CGGTTTCTCACGAGTGGCTATCAGCAGTCCTTAGAAGATTTGGTGAACAATGCCATTTTTGATGAAGGCCATGATGAAAT
GGTCCTCGTTCGAGATATTGATGCCTTTAGTTTGTGTGAGCATCATATGTTGCCCTTTATCGGCAAAGCCCACGTGGCAT
ATATCCCTAATCAAAAGGTGATTGGTCTGAGTAAGCTGGCCCGTATCGTAGAAATGTATTCTCGCCGGCTGCAGGTGCAA
GAAAGATTGACTCGCCAAATTGCAGAAGCAGTACAAACGGCTTTAGAACCGAAGGGTGTGGCTGTGGTTATGGAAGCCAG
CCATATGTGTATGGTGATGCGGGGCGTACAAAAACCAGGTTCCTGGACGGTGACGAGTTCTATGATCGGGGTCTTTCAGG
ACGATCATAAAACCCGAGAAGAGTTCTTAAATCTGATTCGGCATCCCCATAATTTCTAA

Upstream 100 bases:

>100_bases
GAGGAGCTTGTCTTGATGCCGAACGCGGGGACATTCTAAATCTCCTTGTTGTCATCAACCCCCAACTCTTCTGTCAAATT
TCCTACAATTAACTGAAACA

Downstream 100 bases:

>100_bases
GTTGGACTGGTAGGAAAATGCAAATCTGTTCTCTCTTCGGGGCATACTCAGGGTTGATACGCTTTTGTTGAGGGGGGATT
CCTCCCATCTGTTGGAGTTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTTFSPKPSDQANSSMNSTSNNAQLRPDRSLSQRAKEKETHRQASKEEMMGAVETMLLGVGEDPEREGLLKTPKRVAEAM
RFLTSGYQQSLEDLVNNAIFDEGHDEMVLVRDIDAFSLCEHHMLPFIGKAHVAYIPNQKVIGLSKLARIVEMYSRRLQVQ
ERLTRQIAEAVQTALEPKGVAVVMEASHMCMVMRGVQKPGSWTVTSSMIGVFQDDHKTREEFLNLIRHPHNF

Sequences:

>Translated_232_residues
MTTFSPKPSDQANSSMNSTSNNAQLRPDRSLSQRAKEKETHRQASKEEMMGAVETMLLGVGEDPEREGLLKTPKRVAEAM
RFLTSGYQQSLEDLVNNAIFDEGHDEMVLVRDIDAFSLCEHHMLPFIGKAHVAYIPNQKVIGLSKLARIVEMYSRRLQVQ
ERLTRQIAEAVQTALEPKGVAVVMEASHMCMVMRGVQKPGSWTVTSSMIGVFQDDHKTREEFLNLIRHPHNF
>Mature_231_residues
TTFSPKPSDQANSSMNSTSNNAQLRPDRSLSQRAKEKETHRQASKEEMMGAVETMLLGVGEDPEREGLLKTPKRVAEAMR
FLTSGYQQSLEDLVNNAIFDEGHDEMVLVRDIDAFSLCEHHMLPFIGKAHVAYIPNQKVIGLSKLARIVEMYSRRLQVQE
RLTRQIAEAVQTALEPKGVAVVMEASHMCMVMRGVQKPGSWTVTSSMIGVFQDDHKTREEFLNLIRHPHNF

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=209, Percent_Identity=59.8086124401914, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI4503949, Length=209, Percent_Identity=59.8086124401914, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI66932970, Length=178, Percent_Identity=53.3707865168539, Blast_Score=204, Evalue=5e-53,
Organism=Homo sapiens, GI66932972, Length=169, Percent_Identity=55.6213017751479, Blast_Score=204, Evalue=8e-53,
Organism=Escherichia coli, GI1788476, Length=127, Percent_Identity=52.755905511811, Blast_Score=121, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=189, Percent_Identity=64.5502645502645, Blast_Score=259, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6321706, Length=201, Percent_Identity=60.6965174129353, Blast_Score=249, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24656791, Length=189, Percent_Identity=62.4338624338624, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24656782, Length=185, Percent_Identity=63.2432432432432, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24656796, Length=189, Percent_Identity=62.4338624338624, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 26209; Mature: 26078

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFSPKPSDQANSSMNSTSNNAQLRPDRSLSQRAKEKETHRQASKEEMMGAVETMLLGV
CCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDPEREGLLKTPKRVAEAMRFLTSGYQQSLEDLVNNAIFDEGHDEMVLVRDIDAFSLCE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
HHMLPFIGKAHVAYIPNQKVIGLSKLARIVEMYSRRLQVQERLTRQIAEAVQTALEPKGV
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVVMEASHMCMVMRGVQKPGSWTVTSSMIGVFQDDHKTREEFLNLIRHPHNF
EEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTFSPKPSDQANSSMNSTSNNAQLRPDRSLSQRAKEKETHRQASKEEMMGAVETMLLGV
CCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDPEREGLLKTPKRVAEAMRFLTSGYQQSLEDLVNNAIFDEGHDEMVLVRDIDAFSLCE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
HHMLPFIGKAHVAYIPNQKVIGLSKLARIVEMYSRRLQVQERLTRQIAEAVQTALEPKGV
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVVMEASHMCMVMRGVQKPGSWTVTSSMIGVFQDDHKTREEFLNLIRHPHNF
EEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]