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The map label for this gene is gshB [H]

Identifier: 158338672

GI number: 158338672

Start: 5632358

End: 5633335

Strand: Direct

Name: gshB [H]

Synonym: AM1_5576

Alternate gene names: 158338672

Gene position: 5632358-5633335 (Clockwise)

Preceding gene: 158338671

Following gene: 158338673

Centisome position: 86.6

GC content: 47.34

Gene sequence:

>978_bases
GTGAAGCTAGCATTTATCATTGATCCCATTTCTGCCTTAGATCCAGGACACGATACCAGTGTTGCCTTGATGGAATCTGC
ACAAGCCGCTGGCTATGAAGTGTGGATTACCCAATTTGATCAGCTCAGCGTTTTAGATGGACAGGCATGGGCAACCCTAA
CGCCTGTGTCTCTAAAGCCTGTGGAGCTGATCGATGGGTTGTGGGTAGCGACTAAAGACTGGTTTGTATTGGGCGATGCT
CAATTTCAACCTTTGGCCAATATGGATGCTGTATGGATGCGAAAAGATCCCCCAGTGACCGTTTCGTATTTGTACACTAC
CTATCTTTTAGACTACGTTAATCCTCAACAAACCCTCTTAATTAACCATCCTCGGGGTCTAAGGGCTGCTAATGAAAAGA
TGTATGCTCTACAGTTTCAGGGGGCGATTCCTGAAACAATCGTGAGTCAAGATAAGAGCGTGATCCTGCAGTTTGTGCAG
GATAAGGGCATGGCTGTTCTGAAGCCGCTGGGAGGTAAGGCTGGAGAAGGGATCCTACTGCTGCAAGCGGAAGATCGCAA
TCTCAATTCTCTGATTGAGATCAGTACGCGCCGTGGACAGGAGCCAGTGATGGTTCAAGTCTATTTGCCCGAGGCTCAAC
AGGGAGATAAGCGGATTATTCTGCTGGATGGCGAACCCATTGGTGCAGTTAACCGTGTTCCTAGGGGACAAGAGTTTAGG
GGCAATATGGCAACCGGAGGACGAGTAGTTCAAGCTCAAATCACCGATCAGGAAAAATCGATTTGCAGCCAGCTCGGCCC
GGTTCTAAAACGGGATGGTTTGATGTTTGTTGGGATTGATATTATTGGTGGTTACTTAACAGAAGTGAATGTAACGAGTC
CGACGGGGATTCGGGAAATTGATCGCTTAGATAATACGTCTCTAGGCGAAAAGGTGATGCATTGGTTGGTTACTGCTAAA
CAAGGTCTCTTACATTAG

Upstream 100 bases:

>100_bases
AAATCTTCATCAATGATGAACATATTGGGGGGTGTGATGACCTTTATGACTTGGAGGGACAAGGTAAACTGGATCCGCTG
TTGCAAACTTAGGGTGCAGC

Downstream 100 bases:

>100_bases
TCTGCAATAAATGAACAAACCCACTGTATTGGTAACTGGTGGTGCCGGTTACATCGGTTCTCACACTGTTCTGGCACTGC
AACAAGCAGGCTACTCTGTG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKLAFIIDPISALDPGHDTSVALMESAQAAGYEVWITQFDQLSVLDGQAWATLTPVSLKPVELIDGLWVATKDWFVLGDA
QFQPLANMDAVWMRKDPPVTVSYLYTTYLLDYVNPQQTLLINHPRGLRAANEKMYALQFQGAIPETIVSQDKSVILQFVQ
DKGMAVLKPLGGKAGEGILLLQAEDRNLNSLIEISTRRGQEPVMVQVYLPEAQQGDKRIILLDGEPIGAVNRVPRGQEFR
GNMATGGRVVQAQITDQEKSICSQLGPVLKRDGLMFVGIDIIGGYLTEVNVTSPTGIREIDRLDNTSLGEKVMHWLVTAK
QGLLH

Sequences:

>Translated_325_residues
MKLAFIIDPISALDPGHDTSVALMESAQAAGYEVWITQFDQLSVLDGQAWATLTPVSLKPVELIDGLWVATKDWFVLGDA
QFQPLANMDAVWMRKDPPVTVSYLYTTYLLDYVNPQQTLLINHPRGLRAANEKMYALQFQGAIPETIVSQDKSVILQFVQ
DKGMAVLKPLGGKAGEGILLLQAEDRNLNSLIEISTRRGQEPVMVQVYLPEAQQGDKRIILLDGEPIGAVNRVPRGQEFR
GNMATGGRVVQAQITDQEKSICSQLGPVLKRDGLMFVGIDIIGGYLTEVNVTSPTGIREIDRLDNTSLGEKVMHWLVTAK
QGLLH
>Mature_325_residues
MKLAFIIDPISALDPGHDTSVALMESAQAAGYEVWITQFDQLSVLDGQAWATLTPVSLKPVELIDGLWVATKDWFVLGDA
QFQPLANMDAVWMRKDPPVTVSYLYTTYLLDYVNPQQTLLINHPRGLRAANEKMYALQFQGAIPETIVSQDKSVILQFVQ
DKGMAVLKPLGGKAGEGILLLQAEDRNLNSLIEISTRRGQEPVMVQVYLPEAQQGDKRIILLDGEPIGAVNRVPRGQEFR
GNMATGGRVVQAQITDQEKSICSQLGPVLKRDGLMFVGIDIIGGYLTEVNVTSPTGIREIDRLDNTSLGEKVMHWLVTAK
QGLLH

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:
GO:0003824: Glutathione synthase
GO:0004363: Glutathione synthase
GO:0005524: Glutathione synthase
GO:0006750: Glutathione synthase
GO:0016874: Glutathione synthase
GO:0046872: Glutathione synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=302, Percent_Identity=38.7417218543046, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35821; Mature: 35821

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFIIDPISALDPGHDTSVALMESAQAAGYEVWITQFDQLSVLDGQAWATLTPVSLKP
CEEEEEECCHHHCCCCCCCHHHEEHHHCCCCEEEEEEECCEEEEECCCCEEEECCCCCCH
VELIDGLWVATKDWFVLGDAQFQPLANMDAVWMRKDPPVTVSYLYTTYLLDYVNPQQTLL
HHHHCCEEEEECCEEEEECCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCEEEE
INHPRGLRAANEKMYALQFQGAIPETIVSQDKSVILQFVQDKGMAVLKPLGGKAGEGILL
EECCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCEEE
LQAEDRNLNSLIEISTRRGQEPVMVQVYLPEAQQGDKRIILLDGEPIGAVNRVPRGQEFR
EEECCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHCCCCCCCCC
GNMATGGRVVQAQITDQEKSICSQLGPVLKRDGLMFVGIDIIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEHHCCEEEEEEECCCCCHHHH
DRLDNTSLGEKVMHWLVTAKQGLLH
HHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLAFIIDPISALDPGHDTSVALMESAQAAGYEVWITQFDQLSVLDGQAWATLTPVSLKP
CEEEEEECCHHHCCCCCCCHHHEEHHHCCCCEEEEEEECCEEEEECCCCEEEECCCCCCH
VELIDGLWVATKDWFVLGDAQFQPLANMDAVWMRKDPPVTVSYLYTTYLLDYVNPQQTLL
HHHHCCEEEEECCEEEEECCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCEEEE
INHPRGLRAANEKMYALQFQGAIPETIVSQDKSVILQFVQDKGMAVLKPLGGKAGEGILL
EECCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCEEE
LQAEDRNLNSLIEISTRRGQEPVMVQVYLPEAQQGDKRIILLDGEPIGAVNRVPRGQEFR
EEECCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHCCCCCCCCC
GNMATGGRVVQAQITDQEKSICSQLGPVLKRDGLMFVGIDIIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEHHCCEEEEEEECCCCCHHHH
DRLDNTSLGEKVMHWLVTAKQGLLH
HHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]