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The map label for this gene is rnz

Identifier: 158338639

GI number: 158338639

Start: 5591462

End: 5592406

Strand: Reverse

Name: rnz

Synonym: AM1_5543

Alternate gene names: 158338639

Gene position: 5592406-5591462 (Counterclockwise)

Preceding gene: 158338641

Following gene: 158338638

Centisome position: 85.99

GC content: 46.35

Gene sequence:

>945_bases
TTGCAAATTACCTTTTTAGGTACAAGTTCAGGAGTGCCTACGAGATCGCGCAATGTCTCTAGTGTTGCCCTTCGATTGCC
TCAAAGGGCGGAGATATGGTTGTTTGACTGTGGGGAGGGAACTCAACACCAACTGTTGAGGAGTGAGTTGAAGAGCAGCC
AACTCAGACGCATATTTATCACCCATATGCATGGGGACCATATTTTTGGCTTAATGGGGTTATTAGCAAGCTGTGGTCTG
GCTGGTAATACAGAACGGATTGATATCTACGGACCTGCAGGTTTAGAGGAATATTTGCAAGCGTGTCGACGCTATTCTCA
AACGCATTTCTCCTACCCGATACAAGTTCATACGGTTCAACCTGGAGAAGTGTTTTCGGATCAAGATTACTCAGTTGTCT
GTGCGCCTTTGAAGCACCGGGTTCCAGCTTTTGGCTATCGGATACAAGAATGCGATCGCCCTGGCCGTTTCGATACCGAT
AAAGCCATCAGTATGGGAATACCAGCAGGCCCTCTCTATGGACGCCTAAAACGGGGAGAACGCATTACTTTGCCCGATGG
ACGTACTTTTTTTGGCCAAGATTTTTGTGGCCCTAAAGAAATTGGACGAAAAATTGCCTATTGTACCGACACAATTTATT
GTCCAGAGGCAGTTGAGTTGTCTAGGGATGCCGATGTAGTCATTCATGAATCCACCTTTTCCCATCAAGAAGCTGAATTA
GCTTATCAACGCTTGCATTCGACCTCTACAATGGCCGCTCAAGTGGCCCAGGCCGCTGGAGCGAAAAAACTCTTCCTCAC
TCACTTTAGTCCTCGATATGCCCCAAACAGTGCAACTGGTTTGCAAGAGCTTTTAACAGAAGCAAGTGCTATCTTCCCCC
ATACCGAATTGGCCTATGATTTTCTAAATTATCAAGTTCCTCGTCGCACTCTTACAACAGCGTAA

Upstream 100 bases:

>100_bases
AAATACTATTGGCGAGACAATAGTGGCCCGGTTGCCGGGGTGTCACAATAATCGGGGAAAATAGACTAAACGGTAAATCA
GGTATAAGGATAGTCGGGTT

Downstream 100 bases:

>100_bases
AAGGCTTTCTACAGCTGTTTTATCGGAGAAAAAAATAGATAAACGCGACTAGTAGCACATGAGTTTTGGAGAAGAAAGTA
CATCTATTTCCCAATTTCTT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MQITFLGTSSGVPTRSRNVSSVALRLPQRAEIWLFDCGEGTQHQLLRSELKSSQLRRIFITHMHGDHIFGLMGLLASCGL
AGNTERIDIYGPAGLEEYLQACRRYSQTHFSYPIQVHTVQPGEVFSDQDYSVVCAPLKHRVPAFGYRIQECDRPGRFDTD
KAISMGIPAGPLYGRLKRGERITLPDGRTFFGQDFCGPKEIGRKIAYCTDTIYCPEAVELSRDADVVIHESTFSHQEAEL
AYQRLHSTSTMAAQVAQAAGAKKLFLTHFSPRYAPNSATGLQELLTEASAIFPHTELAYDFLNYQVPRRTLTTA

Sequences:

>Translated_314_residues
MQITFLGTSSGVPTRSRNVSSVALRLPQRAEIWLFDCGEGTQHQLLRSELKSSQLRRIFITHMHGDHIFGLMGLLASCGL
AGNTERIDIYGPAGLEEYLQACRRYSQTHFSYPIQVHTVQPGEVFSDQDYSVVCAPLKHRVPAFGYRIQECDRPGRFDTD
KAISMGIPAGPLYGRLKRGERITLPDGRTFFGQDFCGPKEIGRKIAYCTDTIYCPEAVELSRDADVVIHESTFSHQEAEL
AYQRLHSTSTMAAQVAQAAGAKKLFLTHFSPRYAPNSATGLQELLTEASAIFPHTELAYDFLNYQVPRRTLTTA
>Mature_314_residues
MQITFLGTSSGVPTRSRNVSSVALRLPQRAEIWLFDCGEGTQHQLLRSELKSSQLRRIFITHMHGDHIFGLMGLLASCGL
AGNTERIDIYGPAGLEEYLQACRRYSQTHFSYPIQVHTVQPGEVFSDQDYSVVCAPLKHRVPAFGYRIQECDRPGRFDTD
KAISMGIPAGPLYGRLKRGERITLPDGRTFFGQDFCGPKEIGRKIAYCTDTIYCPEAVELSRDADVVIHESTFSHQEAEL
AYQRLHSTSTMAAQVAQAAGAKKLFLTHFSPRYAPNSATGLQELLTEASAIFPHTELAYDFLNYQVPRRTLTTA

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=362, Percent_Identity=34.8066298342541, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI260166619, Length=198, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI87082078, Length=308, Percent_Identity=42.8571428571429, Blast_Score=263, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI71985808, Length=190, Percent_Identity=28.9473684210526, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71985814, Length=190, Percent_Identity=28.9473684210526, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24652403, Length=310, Percent_Identity=28.7096774193548, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_ACAM1 (B0CE23)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519816.1
- ProteinModelPortal:   B0CE23
- SMR:   B0CE23
- GeneID:   5684334
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5543
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- BioCyc:   AMAR329726:AM1_5543-MONOMER
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 35053; Mature: 35053

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITFLGTSSGVPTRSRNVSSVALRLPQRAEIWLFDCGEGTQHQLLRSELKSSQLRRIFI
CEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEE
THMHGDHIFGLMGLLASCGLAGNTERIDIYGPAGLEEYLQACRRYSQTHFSYPIQVHTVQ
EEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
PGEVFSDQDYSVVCAPLKHRVPAFGYRIQECDRPGRFDTDKAISMGIPAGPLYGRLKRGE
CCCCCCCCCCEEEEECHHHCCCHHCCCHHHCCCCCCCCCCCCEECCCCCCHHHHHHCCCC
RITLPDGRTFFGQDFCGPKEIGRKIAYCTDTIYCPEAVELSRDADVVIHESTFSHQEAEL
EEECCCCCCCCCCCCCCHHHHCCEEEHHCCCEECCCHHHCCCCCCEEEECCCCCHHHHHH
AYQRLHSTSTMAAQVAQAAGAKKLFLTHFSPRYAPNSATGLQELLTEASAIFPHTELAYD
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
FLNYQVPRRTLTTA
HHCCCCCCHHCCCC
>Mature Secondary Structure
MQITFLGTSSGVPTRSRNVSSVALRLPQRAEIWLFDCGEGTQHQLLRSELKSSQLRRIFI
CEEEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEE
THMHGDHIFGLMGLLASCGLAGNTERIDIYGPAGLEEYLQACRRYSQTHFSYPIQVHTVQ
EEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
PGEVFSDQDYSVVCAPLKHRVPAFGYRIQECDRPGRFDTDKAISMGIPAGPLYGRLKRGE
CCCCCCCCCCEEEEECHHHCCCHHCCCHHHCCCCCCCCCCCCEECCCCCCHHHHHHCCCC
RITLPDGRTFFGQDFCGPKEIGRKIAYCTDTIYCPEAVELSRDADVVIHESTFSHQEAEL
EEECCCCCCCCCCCCCCHHHHCCEEEHHCCCEECCCHHHCCCCCCEEEECCCCCHHHHHH
AYQRLHSTSTMAAQVAQAAGAKKLFLTHFSPRYAPNSATGLQELLTEASAIFPHTELAYD
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
FLNYQVPRRTLTTA
HHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA