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The map label for this gene is lysS [H]

Identifier: 158338556

GI number: 158338556

Start: 5514863

End: 5516416

Strand: Reverse

Name: lysS [H]

Synonym: AM1_5458

Alternate gene names: 158338556

Gene position: 5516416-5514863 (Counterclockwise)

Preceding gene: 158338557

Following gene: 158338555

Centisome position: 84.82

GC content: 50.32

Gene sequence:

>1554_bases
ATGGCTGCTGATCGGGCGTCTGAGTCACCCAACCAGGGCTCCACTTTAGAAGAAGTGAGAGCTACTCGTTTGCAAAAGGT
TGAGCAGCTCAAACAAGCAGGCTTAACCCCCTATGCCTATCGGTGGCAGTCTACCCATACTGCCCAGGCTCTACAAGAGA
AATTTGTGGATCTGGCGAATGGTGAAGAGGAGGATTTTGAGGTTGCGATCGCAGGTCGTATCCTCGCTCGCCGCGTTTTC
GGTAAACTGGCCTTCTTCAACCTCCAGGACGAAACAGGAACCATCCAGCTCTACTTGGAAAAAAAGCGGATTCAAGAGCA
GATGGGGGAGCAAGATGAGCAAGCCTTTAACCACCTCAAACAGCTCACTGACGTGGGGGACTTCATCGGGGTTCAAGGCA
CTATTAAGCGTACCGATAAAGGTGAACTCTCTGTCTATGTACAGACCTACACTGTGCTCACCAAGTCCTTATTGCCCCTA
CCTGACAAATGGCATGGCTTGACTGATATTGCCAAGCGATATCGCCAACGCTATGTAGATCTGATTGTCAATCCATCTGT
ACGGGAGACGTTTCGACGACGGGCTCAGATCACCGCCAGTATTCGCCGCTATTTAGACGAGCAGGGATTTATTGAAATTG
AAACCCCCGTCTTGAACTCCGAAGCAGGAGGTGCAGAGGCCCGTCCGTTCATCACTTACCACAACACAATGGAGATGGAT
CTGTATCTGCGGATCGCAACGGAACTGCATTTGAAGCGCCTAATTGTTGGCGGTTTTGAAAAAGTCTTTGAACTGGGACG
AGTATTTCGTAACGAAGGGATTTCCACCCGCCACAACCCAGAGTTCACGACCATCGAAGTTTACCAAGCCTATGCCGACT
ACAACGACATGATGGATTTAACGGAAGCGTTGATCTGTACTGCAGCAGAACGTTCTGTGGGCAGTTTGAAAGTGCCTTAC
CAAGGCGAAACCATCGATCTCACCTCTCCGTGGCGACGAGTGACTATGCATGATGCAGTGCAAGAGAAAACGGGGCTAGA
TTTCACCACTTTTGAGACGGTAGAAGCAGCCCAAAAAGCGGCAACGAAGGCAGGCATCACAGGGGTAGACGATTGTGAGA
GTGTGGGGCATGTTTTGAACGAAGCCTTTGAGCAAAAGGTAGAGGCGACTCTAATTCAACCCACCTTTATTCTTGACTAT
CCAGTTGAGATTTCCCCCTTAGCGAAACCCCACCGGTCGCAGTCTGGACTGGTTGAACGATTCGAACTGTTTATCGTGGG
TCGAGAGACCGCCAATAGTTTCTCAGAATTGACGGATCCAGTGGATCAGCGTCAGCGCTTAGAGGCTCAAGCTGCCCGTA
AGGAAGCAGGCGACTTGGAAGCGCACGGTGTGGATGAGGACTTCATCACTGCCTTGGAATATGGCATGCCTCCCACAGGC
GGATTAGGCATTGGTATAGATCGCTTAGCAATGCTGCTGAGTGATGCGGCCAGTATTCGCGATGTGATTGCTTTCCCCCT
CTTAAAACCTGAGAAATCTGAAGATGAAGGATAA

Upstream 100 bases:

>100_bases
TTCCTCTATAATCGAAACTCTGGTATTGCAATCATTCCTGTGGAATGAGTTGTGATTTGATCTAGAACTTGGTTTGAAGT
TTTTCATTTAAGGATTATCT

Downstream 100 bases:

>100_bases
TCGTCGAGGGTAATTGCCAAGTCTTTGTCCAGGCTAAACTGAAGACAGCAAGCTTTTCTCCCCAACCCCCAAAAGATGGT
GTCAACGCCTCAACAACGAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MAADRASESPNQGSTLEEVRATRLQKVEQLKQAGLTPYAYRWQSTHTAQALQEKFVDLANGEEEDFEVAIAGRILARRVF
GKLAFFNLQDETGTIQLYLEKKRIQEQMGEQDEQAFNHLKQLTDVGDFIGVQGTIKRTDKGELSVYVQTYTVLTKSLLPL
PDKWHGLTDIAKRYRQRYVDLIVNPSVRETFRRRAQITASIRRYLDEQGFIEIETPVLNSEAGGAEARPFITYHNTMEMD
LYLRIATELHLKRLIVGGFEKVFELGRVFRNEGISTRHNPEFTTIEVYQAYADYNDMMDLTEALICTAAERSVGSLKVPY
QGETIDLTSPWRRVTMHDAVQEKTGLDFTTFETVEAAQKAATKAGITGVDDCESVGHVLNEAFEQKVEATLIQPTFILDY
PVEISPLAKPHRSQSGLVERFELFIVGRETANSFSELTDPVDQRQRLEAQAARKEAGDLEAHGVDEDFITALEYGMPPTG
GLGIGIDRLAMLLSDAASIRDVIAFPLLKPEKSEDEG

Sequences:

>Translated_517_residues
MAADRASESPNQGSTLEEVRATRLQKVEQLKQAGLTPYAYRWQSTHTAQALQEKFVDLANGEEEDFEVAIAGRILARRVF
GKLAFFNLQDETGTIQLYLEKKRIQEQMGEQDEQAFNHLKQLTDVGDFIGVQGTIKRTDKGELSVYVQTYTVLTKSLLPL
PDKWHGLTDIAKRYRQRYVDLIVNPSVRETFRRRAQITASIRRYLDEQGFIEIETPVLNSEAGGAEARPFITYHNTMEMD
LYLRIATELHLKRLIVGGFEKVFELGRVFRNEGISTRHNPEFTTIEVYQAYADYNDMMDLTEALICTAAERSVGSLKVPY
QGETIDLTSPWRRVTMHDAVQEKTGLDFTTFETVEAAQKAATKAGITGVDDCESVGHVLNEAFEQKVEATLIQPTFILDY
PVEISPLAKPHRSQSGLVERFELFIVGRETANSFSELTDPVDQRQRLEAQAARKEAGDLEAHGVDEDFITALEYGMPPTG
GLGIGIDRLAMLLSDAASIRDVIAFPLLKPEKSEDEG
>Mature_516_residues
AADRASESPNQGSTLEEVRATRLQKVEQLKQAGLTPYAYRWQSTHTAQALQEKFVDLANGEEEDFEVAIAGRILARRVFG
KLAFFNLQDETGTIQLYLEKKRIQEQMGEQDEQAFNHLKQLTDVGDFIGVQGTIKRTDKGELSVYVQTYTVLTKSLLPLP
DKWHGLTDIAKRYRQRYVDLIVNPSVRETFRRRAQITASIRRYLDEQGFIEIETPVLNSEAGGAEARPFITYHNTMEMDL
YLRIATELHLKRLIVGGFEKVFELGRVFRNEGISTRHNPEFTTIEVYQAYADYNDMMDLTEALICTAAERSVGSLKVPYQ
GETIDLTSPWRRVTMHDAVQEKTGLDFTTFETVEAAQKAATKAGITGVDDCESVGHVLNEAFEQKVEATLIQPTFILDYP
VEISPLAKPHRSQSGLVERFELFIVGRETANSFSELTDPVDQRQRLEAQAARKEAGDLEAHGVDEDFITALEYGMPPTGG
LGIGIDRLAMLLSDAASIRDVIAFPLLKPEKSEDEG

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:
GO:0000166: Lysyl-tRNA synthetase
GO:0000287: Lysyl-tRNA synthetase
GO:0003676: Lysyl-tRNA synthetase
GO:0004812: Lysyl-tRNA synthetase
GO:0004824: Lysyl-tRNA synthetase
GO:0005524: Lysyl-tRNA synthetase
GO:0005737: Lysyl-tRNA synthetase
GO:0006412: Lysyl-tRNA synthetase
GO:0006418: Lysyl-tRNA synthetase
GO:0006430: Lysyl-tRNA synthetase
GO:0016874: Lysyl-tRNA synthetase
GO:0046872: Lysyl-tRNA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=524, Percent_Identity=42.9389312977099, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI194272210, Length=524, Percent_Identity=42.9389312977099, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI40789249, Length=272, Percent_Identity=26.8382352941176, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=47.0941883767535, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1789256, Length=494, Percent_Identity=46.7611336032389, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=33.4355828220859, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788173, Length=252, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=513, Percent_Identity=42.495126705653, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535927, Length=513, Percent_Identity=42.495126705653, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=42.8571428571429, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32566633, Length=279, Percent_Identity=24.0143369175627, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=41.6015625, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=485, Percent_Identity=30.9278350515464, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=505, Percent_Identity=25.9405940594059, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24640849, Length=528, Percent_Identity=43.1818181818182, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24640851, Length=527, Percent_Identity=43.2637571157495, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24584738, Length=297, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58153; Mature: 58022

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADRASESPNQGSTLEEVRATRLQKVEQLKQAGLTPYAYRWQSTHTAQALQEKFVDLAN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCC
GEEEDFEVAIAGRILARRVFGKLAFFNLQDETGTIQLYLEKKRIQEQMGEQDEQAFNHLK
CCCCCCEEHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEHHHHHHHHCCHHHHHHHHHH
QLTDVGDFIGVQGTIKRTDKGELSVYVQTYTVLTKSLLPLPDKWHGLTDIAKRYRQRYVD
HHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LIVNPSVRETFRRRAQITASIRRYLDEQGFIEIETPVLNSEAGGAEARPFITYHNTMEMD
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCEEEECCCEEEE
LYLRIATELHLKRLIVGGFEKVFELGRVFRNEGISTRHNPEFTTIEVYQAYADYNDMMDL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCHHHHHHH
TEALICTAAERSVGSLKVPYQGETIDLTSPWRRVTMHDAVQEKTGLDFTTFETVEAAQKA
HHHHHHHHHHCCCCCEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ATKAGITGVDDCESVGHVLNEAFEQKVEATLIQPTFILDYPVEISPLAKPHRSQSGLVER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCCCHHHHHEE
FELFIVGRETANSFSELTDPVDQRQRLEAQAARKEAGDLEAHGVDEDFITALEYGMPPTG
EHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
GLGIGIDRLAMLLSDAASIRDVIAFPLLKPEKSEDEG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AADRASESPNQGSTLEEVRATRLQKVEQLKQAGLTPYAYRWQSTHTAQALQEKFVDLAN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCC
GEEEDFEVAIAGRILARRVFGKLAFFNLQDETGTIQLYLEKKRIQEQMGEQDEQAFNHLK
CCCCCCEEHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEHHHHHHHHCCHHHHHHHHHH
QLTDVGDFIGVQGTIKRTDKGELSVYVQTYTVLTKSLLPLPDKWHGLTDIAKRYRQRYVD
HHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LIVNPSVRETFRRRAQITASIRRYLDEQGFIEIETPVLNSEAGGAEARPFITYHNTMEMD
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCEEEECCCEEEE
LYLRIATELHLKRLIVGGFEKVFELGRVFRNEGISTRHNPEFTTIEVYQAYADYNDMMDL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCHHHHHHH
TEALICTAAERSVGSLKVPYQGETIDLTSPWRRVTMHDAVQEKTGLDFTTFETVEAAQKA
HHHHHHHHHHCCCCCEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ATKAGITGVDDCESVGHVLNEAFEQKVEATLIQPTFILDYPVEISPLAKPHRSQSGLVER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCCCHHHHHEE
FELFIVGRETANSFSELTDPVDQRQRLEAQAARKEAGDLEAHGVDEDFITALEYGMPPTG
EHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
GLGIGIDRLAMLLSDAASIRDVIAFPLLKPEKSEDEG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA