Click here to switch to the map view.

The map label for this gene is mgsA [C]

Identifier: 158338490

GI number: 158338490

Start: 5453990

End: 5455297

Strand: Direct

Name: mgsA [C]

Synonym: AM1_5392

Alternate gene names: 158338490

Gene position: 5453990-5455297 (Clockwise)

Preceding gene: 158338485

Following gene: 158338491

Centisome position: 83.86

GC content: 52.14

Gene sequence:

>1308_bases
ATGGCAGCCACGATCGCATTGATCGCTCACGATCGCAAAAAAGATCAAATCGTTGAATTTGCCAAGCAATACCAACCCGT
TCTCTGCCGATATCGCATTATTGCCACCGGCACGACCGGACAGCGGATTAAAGACGCCACCAGCCTAGAGGTCGAGAGAA
TGCTATCGGGACCTATGGGAGGGGATGCCCAAATTTCCACCCAGGTGGCGGAAGGCAAAGTCACCGCTGTTATTTTCCTC
GTCGATCCGCTCTATGCCCAACCCCATGAGCCCGACATTCAAGCCCTTCAAAGGATTTGCGCCGTTTATGATATCCCTCT
CGCCATCAATGTCGCCACTGCGTCGGCCATCTTAGAGCAACTCCGCCGTACACGAGTCGCCCACCTCATCTTCAATCCCG
TGTCTGGACAAGGCAATGCCGACCATGATTTGACCTTGATCGAAGAACTCCTCAGTCCAGCCATGGAACTGGTCGTGCAT
TTGACTACTCCAGACACCCATCCCAGTGAGTTGGTAGAAGATGCCATGTCAGCCCAAGCCGATCTGATTCTGGCTTCCGG
GGGCGATGGCACGATTTCTGCAGTGGCAGGGGCATTGATCGGTAGCGATATTCCTTTGGGGATTATTCCCCGAGGGACTG
CCAACGCCTTTGCCGCTGCCCTCAATATTTCGCAGGGATTTATGCCGATTCGAAGTGCCTGCAACATTATTGTCCAAGGC
AAGACTTGGGTGGTCGATGTAGCGAAATGCAACGATCGGCCGATGATTCTGTTAGCGGGGATCGGTCTAGAAGCAGAAGT
GATTGAGCGAGCGGACCGAGAAGCCAAAAATCGCTGGGGTGCTTTTGCCTACATTATGGAAGGTATTAAACAGTTCAATC
AGCAGGAGCTTTTTGAAACAGAGATTGAAGTGGAAGGCGTAATCAAAACTTTTAATGCTGGTGCTGTCACGATTGCCAAC
GCCGCACCCCCGACTTCAGTAATGGCTCAAGGGGCAGGGATCGTCGATGCCAGTGATGGACTGTTAGATATCACCATTGG
TGCACCAAAAAATGATTTGGAAGCCGTCGGGGCGATGGTGAACTTAGTGGGGGCTGCCTTGATGCGCCACCCCACCAATC
GGGAAGACATTATCAATCTACAAACCCGTCGAGTCAAAGTGAAAACCCACCCACCCCAAAAGGTTGTCGTGGATGGGGAA
ATGGTGGGAACTACCCCCATCGAAGTGGAATGCATTCCTCGGGGGTTGGTTGTGTTTGCACCTGAGTCAGCAGATTTACA
TCAGGAAGCCCCTGAAACTGAGGCTTAA

Upstream 100 bases:

>100_bases
GTGTAAGCACAAGTTGTATTAGTGCTTGTTCGCAGATGCCAAGCACATAGAACAACGTATGATCGATACAAGACAAAGGT
TTTAAGCACGAGGCAATTGC

Downstream 100 bases:

>100_bases
TTGAATTGAAAGGCATACAGGCTAACGGAAAAACCCTTTATCGCCATGATGAAACTTATGCTCGTGGTGGTCATGATGCT
TGTCGTGGTGCTTTTTCTTC

Product: putative lipid kinase

Products: Methylglyoxal; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAATIALIAHDRKKDQIVEFAKQYQPVLCRYRIIATGTTGQRIKDATSLEVERMLSGPMGGDAQISTQVAEGKVTAVIFL
VDPLYAQPHEPDIQALQRICAVYDIPLAINVATASAILEQLRRTRVAHLIFNPVSGQGNADHDLTLIEELLSPAMELVVH
LTTPDTHPSELVEDAMSAQADLILASGGDGTISAVAGALIGSDIPLGIIPRGTANAFAAALNISQGFMPIRSACNIIVQG
KTWVVDVAKCNDRPMILLAGIGLEAEVIERADREAKNRWGAFAYIMEGIKQFNQQELFETEIEVEGVIKTFNAGAVTIAN
AAPPTSVMAQGAGIVDASDGLLDITIGAPKNDLEAVGAMVNLVGAALMRHPTNREDIINLQTRRVKVKTHPPQKVVVDGE
MVGTTPIEVECIPRGLVVFAPESADLHQEAPETEA

Sequences:

>Translated_435_residues
MAATIALIAHDRKKDQIVEFAKQYQPVLCRYRIIATGTTGQRIKDATSLEVERMLSGPMGGDAQISTQVAEGKVTAVIFL
VDPLYAQPHEPDIQALQRICAVYDIPLAINVATASAILEQLRRTRVAHLIFNPVSGQGNADHDLTLIEELLSPAMELVVH
LTTPDTHPSELVEDAMSAQADLILASGGDGTISAVAGALIGSDIPLGIIPRGTANAFAAALNISQGFMPIRSACNIIVQG
KTWVVDVAKCNDRPMILLAGIGLEAEVIERADREAKNRWGAFAYIMEGIKQFNQQELFETEIEVEGVIKTFNAGAVTIAN
AAPPTSVMAQGAGIVDASDGLLDITIGAPKNDLEAVGAMVNLVGAALMRHPTNREDIINLQTRRVKVKTHPPQKVVVDGE
MVGTTPIEVECIPRGLVVFAPESADLHQEAPETEA
>Mature_434_residues
AATIALIAHDRKKDQIVEFAKQYQPVLCRYRIIATGTTGQRIKDATSLEVERMLSGPMGGDAQISTQVAEGKVTAVIFLV
DPLYAQPHEPDIQALQRICAVYDIPLAINVATASAILEQLRRTRVAHLIFNPVSGQGNADHDLTLIEELLSPAMELVVHL
TTPDTHPSELVEDAMSAQADLILASGGDGTISAVAGALIGSDIPLGIIPRGTANAFAAALNISQGFMPIRSACNIIVQGK
TWVVDVAKCNDRPMILLAGIGLEAEVIERADREAKNRWGAFAYIMEGIKQFNQQELFETEIEVEGVIKTFNAGAVTIANA
APPTSVMAQGAGIVDASDGLLDITIGAPKNDLEAVGAMVNLVGAALMRHPTNREDIINLQTRRVKVKTHPPQKVVVDGEM
VGTTPIEVECIPRGLVVFAPESADLHQEAPETEA

Specific function: Unknown

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI87081809, Length=115, Percent_Identity=48.695652173913, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788402, Length=193, Percent_Identity=28.4974093264249, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR004363
- InterPro:   IPR018148
- InterPro:   IPR011607 [H]

Pfam domain/function: PF00781 DAGK_cat; PF02142 MGS [H]

EC number: 4.2.3.3 [C]

Molecular weight: Translated: 46445; Mature: 46314

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01335 METHYLGLYOXAL_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATIALIAHDRKKDQIVEFAKQYQPVLCRYRIIATGTTGQRIKDATSLEVERMLSGPMG
CCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHCHHHHHHHHHHCCCCC
GDAQISTQVAEGKVTAVIFLVDPLYAQPHEPDIQALQRICAVYDIPLAINVATASAILEQ
CCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
LRRTRVAHLIFNPVSGQGNADHDLTLIEELLSPAMELVVHLTTPDTHPSELVEDAMSAQA
HHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCC
DLILASGGDGTISAVAGALIGSDIPLGIIPRGTANAFAAALNISQGFMPIRSACNIIVQG
CEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCHHHHCCCCEEECC
KTWVVDVAKCNDRPMILLAGIGLEAEVIERADREAKNRWGAFAYIMEGIKQFNQQELFET
CEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
EIEVEGVIKTFNAGAVTIANAAPPTSVMAQGAGIVDASDGLLDITIGAPKNDLEAVGAMV
HHHHEEEEEECCCCEEEEECCCCCHHHHHCCCCEEECCCCEEEEEECCCCHHHHHHHHHH
NLVGAALMRHPTNREDIINLQTRRVKVKTHPPQKVVVDGEMVGTTPIEVECIPRGLVVFA
HHHHHHHHCCCCCHHHEEEEEEEEEEEEECCCCEEEECCEEECCCCEEEEEECCCEEEEC
PESADLHQEAPETEA
CCCCCHHCCCCCCCC
>Mature Secondary Structure 
AATIALIAHDRKKDQIVEFAKQYQPVLCRYRIIATGTTGQRIKDATSLEVERMLSGPMG
CEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHCHHHHHHHHHHCCCCC
GDAQISTQVAEGKVTAVIFLVDPLYAQPHEPDIQALQRICAVYDIPLAINVATASAILEQ
CCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
LRRTRVAHLIFNPVSGQGNADHDLTLIEELLSPAMELVVHLTTPDTHPSELVEDAMSAQA
HHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCC
DLILASGGDGTISAVAGALIGSDIPLGIIPRGTANAFAAALNISQGFMPIRSACNIIVQG
CEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCHHHHCCCCEEECC
KTWVVDVAKCNDRPMILLAGIGLEAEVIERADREAKNRWGAFAYIMEGIKQFNQQELFET
CEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
EIEVEGVIKTFNAGAVTIANAAPPTSVMAQGAGIVDASDGLLDITIGAPKNDLEAVGAMV
HHHHEEEEEECCCCEEEEECCCCCHHHHHCCCCEEECCCCEEEEEECCCCHHHHHHHHHH
NLVGAALMRHPTNREDIINLQTRRVKVKTHPPQKVVVDGEMVGTTPIEVECIPRGLVVFA
HHHHHHHHCCCCCHHHEEEEEEEEEEEEECCCCEEEECCEEECCCCEEEEEECCCEEEEC
PESADLHQEAPETEA
CCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 13200 [C]

Specific activity: NA

Km value (mM): 0.5 {dihydroxyacetone} 0.47 {dihydroxyacetone} 0.2 {dihydroxyacetone} [C]

Substrates: Dihydroxyacetone phosphate [C]

Specific reaction: Dihydroxyacetone phosphate --> Methylglyoxal + Phosphate [C]

General reaction: elimination (C-O bond cleavage [C]

Inhibitor: 2-phosphoglycerate; 3-phosphoglycerate; arsenate; diphosphate; phosphate; phosphoenolpyruvate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]