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The map label for this gene is purA

Identifier: 158338484

GI number: 158338484

Start: 5446911

End: 5448251

Strand: Direct

Name: purA

Synonym: AM1_5386

Alternate gene names: 158338484

Gene position: 5446911-5448251 (Clockwise)

Preceding gene: 158338483

Following gene: 158338485

Centisome position: 83.75

GC content: 51.23

Gene sequence:

>1341_bases
TTGGCTAACGTAGTTGTAATTGGTGCTCAGTGGGGCGATGAAGGAAAGGGCAAAATAACCGATTTGCTGAGTCGTTCAGC
AGATGTGGTAGTTCGGTATCAAGGCGGAGTGAACGCTGGCCATACCGTCGTCGTTAATGACCAGACCTTTAAGTTACATT
TGATTCCTTCCGGCATTCTCTACCCCAACACCGATTGCATCATCGGCTCTGGAACCGTTATTGACCCGAAAGTTCTGATC
GAAGAGCTGGATATGTTAGATAAGCTGGGGGTCAGCACCAGCCATCTGTTTATCTCCGAGTCGGCTCATGTGACGATGCC
CTATCATCGGATGATAGATCAGGCCTCTGAAGAGCGCCGAGGTGACCATAAGATCGGCACCACGGGCAGAGGGATTGGTC
CCACCTATGCTGATAAGTCCGAGCGAACCGGCATCCGTATCCTGGATCTGATGGATCCTGAGGGCATGAAGAAGCAGCTA
CGGTGGACCGTTAACTATAAAAACGTCATTCTCGAAAAACTCTATAATCTGCCTCATTTAGATCCAGAAGAGGTGATTGC
TGAGTATCAAGTCTATGCGGATCGTCTGCGCCCCTTCGTTGCCGACTGTTCCTTAAAAATCTACGATGCCTATCAGCAGC
ACCGGAATATCCTGTTTGAAGGTGCCCAGGGCACGTTACTCGATTTGGATCATGGCACCTATCCCTATGTAACTTCTTCC
AATCCAGTAGCCGGAGGCGCTTGTGTGGGAACAGGGGTTGGCCCCACGATGATTGATCGAGTCATCGGCGTCGCCAAAGC
CTATACCACTCGAGTGGGTGAGGGGCCCTTCCCTACCGAACTCCATGATGAGATGGGAGTTGCCCTCTGTGAGCGAGGTG
CTGAATTTGGAACAACCACTGGACGGCGGCGGCGCTGCGGCTGGTTTGATGCTGTGATTGGTCGCTATGCCGTTCGCATT
AACGGTCTGGACTGTCTAGCGGTAACCAAGCTCGACATCTTAGACGAGGTGGATGAAATTAAAGTTTGCGTGGCCTATGA
AATTGATGGCCACGAGTCGAAGGACTTTCCCACTAATGCTCGCCAGTTTTCTCGCTGCAACCCCGTGTATAAAACCCTAC
CGGGCTGGAAACAATCCACGATTCATTGCCGCACTTTGGAAGACTTACCCCCCAAAGCCCTAGACTACCTGAAGTTTTTA
GCTAGCATTGTTGAAGTCCCGATTGCGATTGTGTCATTAGGGGCTGAGCGGGACGAGACCATTATCGTTGAAGATCCCAT
TCATGGACCGAAGCGAGCCTTGCTCTATTCCAATGGGGAATCCCAAGCCATGTCTGCTTAG

Upstream 100 bases:

>100_bases
CCCTCAGGAGTCGGGAGAGATTCTGACAGTGAGATTGACAGAAGTCTCCCCCACTACCGATAATTGTTGTTTGTTAAATC
GTTGTTTTTTAAATAATCCC

Downstream 100 bases:

>100_bases
GCCGTTTTCCAAAACTAACCTAAGTCATATCGCTTTATTCCCTCTAGACTTTGTAGCTTGCGATCGCAACCCTGATTCTT
CCAAACTCTGATTTGGCGTC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTVVVNDQTFKLHLIPSGILYPNTDCIIGSGTVIDPKVLI
EELDMLDKLGVSTSHLFISESAHVTMPYHRMIDQASEERRGDHKIGTTGRGIGPTYADKSERTGIRILDLMDPEGMKKQL
RWTVNYKNVILEKLYNLPHLDPEEVIAEYQVYADRLRPFVADCSLKIYDAYQQHRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGTGVGPTMIDRVIGVAKAYTTRVGEGPFPTELHDEMGVALCERGAEFGTTTGRRRRCGWFDAVIGRYAVRI
NGLDCLAVTKLDILDEVDEIKVCVAYEIDGHESKDFPTNARQFSRCNPVYKTLPGWKQSTIHCRTLEDLPPKALDYLKFL
ASIVEVPIAIVSLGAERDETIIVEDPIHGPKRALLYSNGESQAMSA

Sequences:

>Translated_446_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTVVVNDQTFKLHLIPSGILYPNTDCIIGSGTVIDPKVLI
EELDMLDKLGVSTSHLFISESAHVTMPYHRMIDQASEERRGDHKIGTTGRGIGPTYADKSERTGIRILDLMDPEGMKKQL
RWTVNYKNVILEKLYNLPHLDPEEVIAEYQVYADRLRPFVADCSLKIYDAYQQHRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGTGVGPTMIDRVIGVAKAYTTRVGEGPFPTELHDEMGVALCERGAEFGTTTGRRRRCGWFDAVIGRYAVRI
NGLDCLAVTKLDILDEVDEIKVCVAYEIDGHESKDFPTNARQFSRCNPVYKTLPGWKQSTIHCRTLEDLPPKALDYLKFL
ASIVEVPIAIVSLGAERDETIIVEDPIHGPKRALLYSNGESQAMSA
>Mature_445_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTVVVNDQTFKLHLIPSGILYPNTDCIIGSGTVIDPKVLIE
ELDMLDKLGVSTSHLFISESAHVTMPYHRMIDQASEERRGDHKIGTTGRGIGPTYADKSERTGIRILDLMDPEGMKKQLR
WTVNYKNVILEKLYNLPHLDPEEVIAEYQVYADRLRPFVADCSLKIYDAYQQHRNILFEGAQGTLLDLDHGTYPYVTSSN
PVAGGACVGTGVGPTMIDRVIGVAKAYTTRVGEGPFPTELHDEMGVALCERGAEFGTTTGRRRRCGWFDAVIGRYAVRIN
GLDCLAVTKLDILDEVDEIKVCVAYEIDGHESKDFPTNARQFSRCNPVYKTLPGWKQSTIHCRTLEDLPPKALDYLKFLA
SIVEVPIAIVSLGAERDETIIVEDPIHGPKRALLYSNGESQAMSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=45.1764705882353, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=43.559718969555, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=43.4343434343434, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=49.6487119437939, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=43.0913348946136, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=43.0913348946136, Blast_Score=335, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.6194895591647, Blast_Score=349, Evalue=6e-97,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=42.688679245283, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ACAM1 (B0CBU2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519661.1
- ProteinModelPortal:   B0CBU2
- SMR:   B0CBU2
- GeneID:   5684178
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5386
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- BioCyc:   AMAR329726:AM1_5386-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 49193; Mature: 49061

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTVVVNDQTFKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPNTDCIIGSGTVIDPKVLIEELDMLDKLGVSTSHLFISESAHVTMPYHRMIDQASEERR
CCCCCEEECCCCEECHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHHHHC
GDHKIGTTGRGIGPTYADKSERTGIRILDLMDPEGMKKQLRWTVNYKNVILEKLYNLPHL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEEEECHHHHHHHHHHCCCCC
DPEEVIAEYQVYADRLRPFVADCSLKIYDAYQQHRNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHHHHCCEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGTGVGPTMIDRVIGVAKAYTTRVGEGPFPTELHDEMGVALCERGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GRRRRCGWFDAVIGRYAVRINGLDCLAVTKLDILDEVDEIKVCVAYEIDGHESKDFPTNA
CCCCCCCHHHHHHHCEEEEECCCEEEEEHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCH
RQFSRCNPVYKTLPGWKQSTIHCRTLEDLPPKALDYLKFLASIVEVPIAIVSLGAERDET
HHHHHCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IIVEDPIHGPKRALLYSNGESQAMSA
EEEECCCCCCCEEEEEECCCHHCCCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTVVVNDQTFKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPNTDCIIGSGTVIDPKVLIEELDMLDKLGVSTSHLFISESAHVTMPYHRMIDQASEERR
CCCCCEEECCCCEECHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHHHHC
GDHKIGTTGRGIGPTYADKSERTGIRILDLMDPEGMKKQLRWTVNYKNVILEKLYNLPHL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEEEECHHHHHHHHHHCCCCC
DPEEVIAEYQVYADRLRPFVADCSLKIYDAYQQHRNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHHHHCCEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGTGVGPTMIDRVIGVAKAYTTRVGEGPFPTELHDEMGVALCERGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GRRRRCGWFDAVIGRYAVRINGLDCLAVTKLDILDEVDEIKVCVAYEIDGHESKDFPTNA
CCCCCCCHHHHHHHCEEEEECCCEEEEEHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCH
RQFSRCNPVYKTLPGWKQSTIHCRTLEDLPPKALDYLKFLASIVEVPIAIVSLGAERDET
HHHHHCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IIVEDPIHGPKRALLYSNGESQAMSA
EEEECCCCCCCEEEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA