Click here to switch to the map view.

The map label for this gene is yfcG [H]

Identifier: 158338445

GI number: 158338445

Start: 5405128

End: 5405919

Strand: Direct

Name: yfcG [H]

Synonym: AM1_5345

Alternate gene names: 158338445

Gene position: 5405128-5405919 (Clockwise)

Preceding gene: 158338444

Following gene: 158338447

Centisome position: 83.11

GC content: 43.81

Gene sequence:

>792_bases
GTGGCTACATCATTCTTAAGCTTGATAACCTTTAGTTCGATCGATAACGCGGCAAGGGATAAGATAATTGAGTGCTTCTT
AAGCATTAAGAATATGACACAACTATTGCCTGTCAAAAAGATAATTTTCCTGACTCTTGGCCTTATCAGCATAAGTCAAT
CTACCGGACAAAATCTCGCACCAGCAATAGCACAAAACGCTTCATTAATCCCTTCGGAGTTTACCCAAAAACAGTCTGAT
TTATTGCTTTATGGGGGGCCTCGAACCAGATCTCCATTAGTGCAATGGTATCTCGAAGAACTAGCCGTCTCCTATCAGTA
TATTTCTTTAGATATCCGGGGGCAGGAACAGCGGCAGCCTGAATTTTTAGCCATCAATCCTATGGGAAAAGTTCCAGCAA
TGGTAGATGGAACTTTTAAATTATGGGAGTCCGGTGCCATTCTCCTGTATTTAACTGACAAATACGGAAAAGAGCCTCAA
TCTATCGAAGAAAGAGCCCTCTTAAACCAGTGGGTGATCTTCGCCAATGCCACCTTAGGACCCGGGCTATTTCGCGAAGA
TCGACGAGAACGGGAAATGCCAAGATTACTGGCACCTTTAAATGACATCTTTAAACAACAGCCCTTTATCCTTGGCTCTG
AACTCAGTGTCGCAGATGTAGCGGTAGGTTCATACTTGTACTATGCCAAACTCGGGCTATCTCTAGATTTTAGCGACTAT
CCAGCAGTCGAGACCTATCTCAATCGCCTCTCAAAGCGACCGGCATTTATCAAGACCATGGGACAGCGATAA

Upstream 100 bases:

>100_bases
ATCCACAGATTTCGAAACTCTTCTGTCCAGGGCTGGGAACAGGCATCGGCTGTGTTGAACCTCATCTTGCCGCCCAGGAG
ATGGCCATGGCTTACCGAAA

Downstream 100 bases:

>100_bases
AGTCGCACCAATCGACACTGGCCATGACTCCCTCAGTGGTGGCTCATCCGTTTACGAGTGATTGGAGAGTTGGATCCGTA
AAGTCTTGGATACAGTATTC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MATSFLSLITFSSIDNAARDKIIECFLSIKNMTQLLPVKKIIFLTLGLISISQSTGQNLAPAIAQNASLIPSEFTQKQSD
LLLYGGPRTRSPLVQWYLEELAVSYQYISLDIRGQEQRQPEFLAINPMGKVPAMVDGTFKLWESGAILLYLTDKYGKEPQ
SIEERALLNQWVIFANATLGPGLFREDRREREMPRLLAPLNDIFKQQPFILGSELSVADVAVGSYLYYAKLGLSLDFSDY
PAVETYLNRLSKRPAFIKTMGQR

Sequences:

>Translated_263_residues
MATSFLSLITFSSIDNAARDKIIECFLSIKNMTQLLPVKKIIFLTLGLISISQSTGQNLAPAIAQNASLIPSEFTQKQSD
LLLYGGPRTRSPLVQWYLEELAVSYQYISLDIRGQEQRQPEFLAINPMGKVPAMVDGTFKLWESGAILLYLTDKYGKEPQ
SIEERALLNQWVIFANATLGPGLFREDRREREMPRLLAPLNDIFKQQPFILGSELSVADVAVGSYLYYAKLGLSLDFSDY
PAVETYLNRLSKRPAFIKTMGQR
>Mature_262_residues
ATSFLSLITFSSIDNAARDKIIECFLSIKNMTQLLPVKKIIFLTLGLISISQSTGQNLAPAIAQNASLIPSEFTQKQSDL
LLYGGPRTRSPLVQWYLEELAVSYQYISLDIRGQEQRQPEFLAINPMGKVPAMVDGTFKLWESGAILLYLTDKYGKEPQS
IEERALLNQWVIFANATLGPGLFREDRREREMPRLLAPLNDIFKQQPFILGSELSVADVAVGSYLYYAKLGLSLDFSDYP
AVETYLNRLSKRPAFIKTMGQR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=196, Percent_Identity=32.6530612244898, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081789, Length=162, Percent_Identity=30.2469135802469, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082195, Length=190, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324100, Length=94, Percent_Identity=37.2340425531915, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571670, Length=153, Percent_Identity=30.718954248366, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130115, Length=203, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17864596, Length=184, Percent_Identity=28.2608695652174, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17933730, Length=154, Percent_Identity=33.1168831168831, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654965, Length=200, Percent_Identity=27.5, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654975, Length=196, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922526, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864592, Length=153, Percent_Identity=32.0261437908497, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864594, Length=159, Percent_Identity=30.8176100628931, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646251, Length=190, Percent_Identity=31.0526315789474, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922532, Length=203, Percent_Identity=28.0788177339901, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646249, Length=128, Percent_Identity=32.8125, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549270, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI85725204, Length=129, Percent_Identity=34.8837209302326, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737923, Length=129, Percent_Identity=34.8837209302326, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654983, Length=197, Percent_Identity=28.9340101522843, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24654992, Length=205, Percent_Identity=25.8536585365854, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864598, Length=150, Percent_Identity=28, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 29613; Mature: 29481

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSFLSLITFSSIDNAARDKIIECFLSIKNMTQLLPVKKIIFLTLGLISISQSTGQNLA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAIAQNASLIPSEFTQKQSDLLLYGGPRTRSPLVQWYLEELAVSYQYISLDIRGQEQRQP
HHHHCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCC
EFLAINPMGKVPAMVDGTFKLWESGAILLYLTDKYGKEPQSIEERALLNQWVIFANATLG
CEEEECCCCCCCCEECCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCC
PGLFREDRREREMPRLLAPLNDIFKQQPFILGSELSVADVAVGSYLYYAKLGLSLDFSDY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCEECCCCC
PAVETYLNRLSKRPAFIKTMGQR
CHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
ATSFLSLITFSSIDNAARDKIIECFLSIKNMTQLLPVKKIIFLTLGLISISQSTGQNLA
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAIAQNASLIPSEFTQKQSDLLLYGGPRTRSPLVQWYLEELAVSYQYISLDIRGQEQRQP
HHHHCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCC
EFLAINPMGKVPAMVDGTFKLWESGAILLYLTDKYGKEPQSIEERALLNQWVIFANATLG
CEEEECCCCCCCCEECCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCC
PGLFREDRREREMPRLLAPLNDIFKQQPFILGSELSVADVAVGSYLYYAKLGLSLDFSDY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCEECCCCC
PAVETYLNRLSKRPAFIKTMGQR
CHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]