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The map label for this gene is ilvE [H]

Identifier: 158338430

GI number: 158338430

Start: 5395086

End: 5396045

Strand: Direct

Name: ilvE [H]

Synonym: AM1_5330

Alternate gene names: 158338430

Gene position: 5395086-5396045 (Clockwise)

Preceding gene: 158338423

Following gene: 158338432

Centisome position: 82.95

GC content: 48.75

Gene sequence:

>960_bases
ATGACACTCCTTACTCCACTAGATTCACTAACCACGGCTTCTGCTCCAGCATTTCTGCCCATTGCCTTTTTTGAAGGGGA
GTTCTGCCCTTTCCCTGAAGCTCAACTTTCAATTGCGACGCACGCGCTTCACTATGGCACTTCAGTTTTGGGTGGGATTC
GTGGACTACCTTCTCCTAGTGACTCCAACCAGGTTTTGGTGTTTCGCCTAGCGGATCACTGTCGGCGATTGAGCCAGAGT
GCTAAATATCTGAATACCGATATTGCCCCCATGGAGCTGGAAGCAACATTGATTGAATTTGTTCGGCGAAACCAGCCCCA
GACTTCCTTCTATATCCGACCGTTGATCTACACTTCCAGCTTGGGCATTGCTCCTCGGCTGCATGATGTCAGCAAAGATC
TATTGATCTATGGCCTGGAGATGGGGGGTTACCTGCACCAAGGGGGTGTGCGTTGCCGTATCAGTTCTTGGCTACGACAA
GAGGATCGTAGTCAGCCCTTGCGGGGCAAGTCGAGTGCCGGATATATTGCATCGGCCTTAGCTAAAACCGAGGCGGTTGC
ATCTGGGTTTGATGAAGCAATTTTGATGAACGCTCGGGGTAAGGTTAGCGAGGCATCAGCCATGAATCTGTTTTTAGTTA
GAAATGGCCAGCTGATTACGCCCAGTGTTGATCAAGACATTTTAGAAGGCATTACCCGTGCCAGCATTATCACGCTGGCA
AAGGATTCAGGGATTCCTGTTATCGAGCGTCCTGTTGATAAGTCTGAGCTTTTTATCGCCGATGAAGTCTTTCTTTGTGG
CACAGCAGCGCAAATTTCACCAGTGTTAGAAATTGAAACCTACAAGCTCCCAGCACAACGTCCTGTTTCAGACCAGTTAG
GTAAATTGATACGAAACGTGATGATAGGAGCAGAGCAACGCTACTCCAACTGGCTCAGCGTTGTACATCTCAATTCTTAG

Upstream 100 bases:

>100_bases
TACAAGGTTTTCCAAGTACTGGGCATCGCTCATTAGATGACACCACTAAAGAGTGGTGCTATTTTAAAGTTCATCCTGAA
CCTTTTGCAAGTGCTGTATC

Downstream 100 bases:

>100_bases
TCAGATTACTCAGATACAGCGAAGATTCATCCGCCATAGGTTTATTCTGCTTAAATCTAGATCTCGAAGCTTTGAAGGGC
AAGCATCCAAACAGACTGTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTLLTPLDSLTTASAPAFLPIAFFEGEFCPFPEAQLSIATHALHYGTSVLGGIRGLPSPSDSNQVLVFRLADHCRRLSQS
AKYLNTDIAPMELEATLIEFVRRNQPQTSFYIRPLIYTSSLGIAPRLHDVSKDLLIYGLEMGGYLHQGGVRCRISSWLRQ
EDRSQPLRGKSSAGYIASALAKTEAVASGFDEAILMNARGKVSEASAMNLFLVRNGQLITPSVDQDILEGITRASIITLA
KDSGIPVIERPVDKSELFIADEVFLCGTAAQISPVLEIETYKLPAQRPVSDQLGKLIRNVMIGAEQRYSNWLSVVHLNS

Sequences:

>Translated_319_residues
MTLLTPLDSLTTASAPAFLPIAFFEGEFCPFPEAQLSIATHALHYGTSVLGGIRGLPSPSDSNQVLVFRLADHCRRLSQS
AKYLNTDIAPMELEATLIEFVRRNQPQTSFYIRPLIYTSSLGIAPRLHDVSKDLLIYGLEMGGYLHQGGVRCRISSWLRQ
EDRSQPLRGKSSAGYIASALAKTEAVASGFDEAILMNARGKVSEASAMNLFLVRNGQLITPSVDQDILEGITRASIITLA
KDSGIPVIERPVDKSELFIADEVFLCGTAAQISPVLEIETYKLPAQRPVSDQLGKLIRNVMIGAEQRYSNWLSVVHLNS
>Mature_318_residues
TLLTPLDSLTTASAPAFLPIAFFEGEFCPFPEAQLSIATHALHYGTSVLGGIRGLPSPSDSNQVLVFRLADHCRRLSQSA
KYLNTDIAPMELEATLIEFVRRNQPQTSFYIRPLIYTSSLGIAPRLHDVSKDLLIYGLEMGGYLHQGGVRCRISSWLRQE
DRSQPLRGKSSAGYIASALAKTEAVASGFDEAILMNARGKVSEASAMNLFLVRNGQLITPSVDQDILEGITRASIITLAK
DSGIPVIERPVDKSELFIADEVFLCGTAAQISPVLEIETYKLPAQRPVSDQLGKLIRNVMIGAEQRYSNWLSVVHLNS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=303, Percent_Identity=34.6534653465347, Blast_Score=168, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322608, Length=325, Percent_Identity=24.6153846153846, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322002, Length=338, Percent_Identity=26.0355029585799, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34920; Mature: 34788

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLTPLDSLTTASAPAFLPIAFFEGEFCPFPEAQLSIATHALHYGTSVLGGIRGLPSPS
CCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DSNQVLVFRLADHCRRLSQSAKYLNTDIAPMELEATLIEFVRRNQPQTSFYIRPLIYTSS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
LGIAPRLHDVSKDLLIYGLEMGGYLHQGGVRCRISSWLRQEDRSQPLRGKSSAGYIASAL
CCCCCCHHHCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHH
AKTEAVASGFDEAILMNARGKVSEASAMNLFLVRNGQLITPSVDQDILEGITRASIITLA
HHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHEEEEE
KDSGIPVIERPVDKSELFIADEVFLCGTAAQISPVLEIETYKLPAQRPVSDQLGKLIRNV
CCCCCCEEECCCCCCCEEEECCEEEECCHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHH
MIGAEQRYSNWLSVVHLNS
HHCCHHHHCCCEEEEEECC
>Mature Secondary Structure 
TLLTPLDSLTTASAPAFLPIAFFEGEFCPFPEAQLSIATHALHYGTSVLGGIRGLPSPS
CCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DSNQVLVFRLADHCRRLSQSAKYLNTDIAPMELEATLIEFVRRNQPQTSFYIRPLIYTSS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
LGIAPRLHDVSKDLLIYGLEMGGYLHQGGVRCRISSWLRQEDRSQPLRGKSSAGYIASAL
CCCCCCHHHCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHH
AKTEAVASGFDEAILMNARGKVSEASAMNLFLVRNGQLITPSVDQDILEGITRASIITLA
HHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHEEEEE
KDSGIPVIERPVDKSELFIADEVFLCGTAAQISPVLEIETYKLPAQRPVSDQLGKLIRNV
CCCCCCEEECCCCCCCEEEECCEEEECCHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHH
MIGAEQRYSNWLSVVHLNS
HHCCHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]