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The map label for this gene is dut

Identifier: 158338327

GI number: 158338327

Start: 5287154

End: 5287585

Strand: Direct

Name: dut

Synonym: AM1_5223

Alternate gene names: 158338327

Gene position: 5287154-5287585 (Clockwise)

Preceding gene: 158338326

Following gene: 158338334

Centisome position: 81.29

GC content: 47.45

Gene sequence:

>432_bases
ATGAAAATCAAAATTATCAAGTTAACAGAATCAGCCCAAGTCCCTCGCTATAGCCATGCTGATGATGCAGGACTAGATCT
ATTTGCCATCGAAGCCCAGAAAATCTTGCCGGGTGCATCTGCCCTGATTCCCACTGGAATTGCCATCGAGTTACCACAGG
GAACTGAAGCCCAGGTGCGTCCGCGCAGTGGATTAGCCCTCAAGCATTCCATTACGGTATTGAATTCGCCGGGAACCATT
GATGCGGGGTATCGCGGTGAAATTGGGGTGATCTTAATTAACCACGGTCAAGAAACCTTTCAGGTGGTTGAAGGGATGAA
AATTGCTCAAATGGTGATTGCACCAATTATGCGAGCCGAGATTGAAGAAGTAACTGAATTAAGCGCCACCCAAAGAGGGG
AAGGGGGATTTGGCTCAACTGGATATGCTTAA

Upstream 100 bases:

>100_bases
CATTTTATCCAGTTCCAGAGTGTTGTTTTACTCCTCTGTAAGCATTGCAAAATTTGGATGAATAACAAAGGCTGTAAGCT
AATGTGCCACCACACCACCA

Downstream 100 bases:

>100_bases
AGCGGGTCAACGGGACACACAAATGCTTGGCCAAGCTTTGATGGGGAAGGGGATATCCAACCTTAAGGTAGTGAGGTAAA
GCAGTGATTATCGATGGGGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKIKIIKLTESAQVPRYSHADDAGLDLFAIEAQKILPGASALIPTGIAIELPQGTEAQVRPRSGLALKHSITVLNSPGTI
DAGYRGEIGVILINHGQETFQVVEGMKIAQMVIAPIMRAEIEEVTELSATQRGEGGFGSTGYA

Sequences:

>Translated_143_residues
MKIKIIKLTESAQVPRYSHADDAGLDLFAIEAQKILPGASALIPTGIAIELPQGTEAQVRPRSGLALKHSITVLNSPGTI
DAGYRGEIGVILINHGQETFQVVEGMKIAQMVIAPIMRAEIEEVTELSATQRGEGGFGSTGYA
>Mature_143_residues
MKIKIIKLTESAQVPRYSHADDAGLDLFAIEAQKILPGASALIPTGIAIELPQGTEAQVRPRSGLALKHSITVLNSPGTI
DAGYRGEIGVILINHGQETFQVVEGMKIAQMVIAPIMRAEIEEVTELSATQRGEGGFGSTGYA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:
GO:0004170: Deoxyuridine 5'-triphosphate nucleotidohydrolase
GO:0009117: Deoxyuridine 5'-triphosphate nucleotidohydrolase
GO:0016787: Deoxyuridine 5'-triphosphate nucleotidohydrolase
GO:0046080: Deoxyuridine 5'-triphosphate nucleotidohydrolase
GO:0046872: Deoxyuridine 5'-triphosphate nucleotidohydrolase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=141, Percent_Identity=46.0992907801418, Blast_Score=122, Evalue=1e-28,
Organism=Homo sapiens, GI4503423, Length=141, Percent_Identity=46.0992907801418, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI70906441, Length=141, Percent_Identity=46.0992907801418, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1790071, Length=144, Percent_Identity=39.5833333333333, Blast_Score=106, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=141, Percent_Identity=43.2624113475177, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319729, Length=140, Percent_Identity=37.8571428571429, Blast_Score=91, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583610, Length=139, Percent_Identity=38.8489208633094, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19921126, Length=139, Percent_Identity=38.8489208633094, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_ACAM1 (B0C9N7)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519504.1
- ProteinModelPortal:   B0C9N7
- SMR:   B0C9N7
- GeneID:   5684019
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5223
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- BioCyc:   AMAR329726:AM1_5223-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15153; Mature: 15153

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: BINDING 75-75

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKIIKLTESAQVPRYSHADDAGLDLFAIEAQKILPGASALIPTGIAIELPQGTEAQVR
CEEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCEEEECCCCCCCEEC
PRSGLALKHSITVLNSPGTIDAGYRGEIGVILINHGQETFQVVEGMKIAQMVIAPIMRAE
CCCCEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
IEEVTELSATQRGEGGFGSTGYA
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIKIIKLTESAQVPRYSHADDAGLDLFAIEAQKILPGASALIPTGIAIELPQGTEAQVR
CEEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCEEEECCCCCCCEEC
PRSGLALKHSITVLNSPGTIDAGYRGEIGVILINHGQETFQVVEGMKIAQMVIAPIMRAE
CCCCEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
IEEVTELSATQRGEGGFGSTGYA
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA