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The map label for this gene is yjfH [C]

Identifier: 158338300

GI number: 158338300

Start: 5259347

End: 5260276

Strand: Reverse

Name: yjfH [C]

Synonym: AM1_5196

Alternate gene names: 158338300

Gene position: 5260276-5259347 (Counterclockwise)

Preceding gene: 158338301

Following gene: 158338299

Centisome position: 80.88

GC content: 49.25

Gene sequence:

>930_bases
ATGACCAAAGCGCGGCCATCCCATAATAATCACGGTAAAAGTAAGCCGCGCAAACCTCAGCGCTCTTATTCATCAGCCAA
AAAGCCCCATCGTCAATCGACGTCCTCACGGCCACCTCAGAAACCTAGAATACGCTCTAAAGATAAAGAGCCTGCCCAGT
CTGAAGAGGAGCAGGGAGATTTAATTTATGGACGCCATTCAGTTCTGTCAGCCCTGGAGCATCAACGATCTTTAAATCGG
GTATGGGTTGTGTCCCGTCTCCGTTACGATCCCCGCTTTCATAGTCTTCTCAACCAAGCCAAATCTCAAGGCACCATTAT
TGATGAGGTGGATCATCGGCGACTGAATCAACTGACCCAGCAAGCAACCCACCAAGGGATCGCTGCTCAAGTGGCGGCCT
ATGAATATTTAGATATCTCGACGTTGATAGAGCAAGCTAACGCTGCTACCCCTAAACCCGTCATTATTGCTGCTGATAGT
ATTACTGATCCCCATAATCTAGGAGCTATGATTCGAACGGCTGAGGCTTTAGGGGCGCAGGGGGTGATTATTCCCCAAAG
GCGAGCTGCGGGGATCACCTCAGTGGTTGCAAAAGTAGCGACTGGGGCCTTAGAAACTTTGGCGATTGCTCGGGTGGTTA
ACCTTAACCAGGGACTTGAGCAGCTTAAAAAAGCTGGATTTTGGATTTATGGGTTGAGTACTTCAGCACCACAATCTATC
CATACCGTGACCTTTACAGAGCCCACTGTATTGGTGATTGGCGCTGAAGGTCCAGGGTTGAGTGTTTCCACTCAGCATCA
TTGTGACCAAATGGTGTCGATCCCATTACAGGGGAAAACAGCCAGCTTGAATGCGTCTGTGGCAACGGGTATGGCCCTTT
ATGAGGTCTATCGTCAACGTTGGGTAGAAACCCTCCATTTGCATCCTTGA

Upstream 100 bases:

>100_bases
TTAAGTTTGATCGCTCATCGCCTGATCCTGCTTAAAATAGCTTCGGTTTAATTTTGCCCTGGACGTAGTATTTGTCCCGT
AGCACGAGAGTTTCATCCCT

Downstream 100 bases:

>100_bases
CGTATACAGATGTTACAGAATATAAAGAGGCTCTACCCTAAACTATTAGCCGAGATGGTTTGAAATTAGGATCTAGCATT
GCAGAGGTAAATGTTTTCAC

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTKARPSHNNHGKSKPRKPQRSYSSAKKPHRQSTSSRPPQKPRIRSKDKEPAQSEEEQGDLIYGRHSVLSALEHQRSLNR
VWVVSRLRYDPRFHSLLNQAKSQGTIIDEVDHRRLNQLTQQATHQGIAAQVAAYEYLDISTLIEQANAATPKPVIIAADS
ITDPHNLGAMIRTAEALGAQGVIIPQRRAAGITSVVAKVATGALETLAIARVVNLNQGLEQLKKAGFWIYGLSTSAPQSI
HTVTFTEPTVLVIGAEGPGLSVSTQHHCDQMVSIPLQGKTASLNASVATGMALYEVYRQRWVETLHLHP

Sequences:

>Translated_309_residues
MTKARPSHNNHGKSKPRKPQRSYSSAKKPHRQSTSSRPPQKPRIRSKDKEPAQSEEEQGDLIYGRHSVLSALEHQRSLNR
VWVVSRLRYDPRFHSLLNQAKSQGTIIDEVDHRRLNQLTQQATHQGIAAQVAAYEYLDISTLIEQANAATPKPVIIAADS
ITDPHNLGAMIRTAEALGAQGVIIPQRRAAGITSVVAKVATGALETLAIARVVNLNQGLEQLKKAGFWIYGLSTSAPQSI
HTVTFTEPTVLVIGAEGPGLSVSTQHHCDQMVSIPLQGKTASLNASVATGMALYEVYRQRWVETLHLHP
>Mature_308_residues
TKARPSHNNHGKSKPRKPQRSYSSAKKPHRQSTSSRPPQKPRIRSKDKEPAQSEEEQGDLIYGRHSVLSALEHQRSLNRV
WVVSRLRYDPRFHSLLNQAKSQGTIIDEVDHRRLNQLTQQATHQGIAAQVAAYEYLDISTLIEQANAATPKPVIIAADSI
TDPHNLGAMIRTAEALGAQGVIIPQRRAAGITSVVAKVATGALETLAIARVVNLNQGLEQLKKAGFWIYGLSTSAPQSIH
TVTFTEPTVLVIGAEGPGLSVSTQHHCDQMVSIPLQGKTASLNASVATGMALYEVYRQRWVETLHLHP

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=258, Percent_Identity=28.2945736434109, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=37.2950819672131, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1788935, Length=240, Percent_Identity=25.8333333333333, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=30.8724832214765, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=267, Percent_Identity=29.5880149812734, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324775, Length=287, Percent_Identity=26.8292682926829, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 33904; Mature: 33773

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKARPSHNNHGKSKPRKPQRSYSSAKKPHRQSTSSRPPQKPRIRSKDKEPAQSEEEQGD
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LIYGRHSVLSALEHQRSLNRVWVVSRLRYDPRFHSLLNQAKSQGTIIDEVDHRRLNQLTQ
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QATHQGIAAQVAAYEYLDISTLIEQANAATPKPVIIAADSITDPHNLGAMIRTAEALGAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
GVIIPQRRAAGITSVVAKVATGALETLAIARVVNLNQGLEQLKKAGFWIYGLSTSAPQSI
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCE
HTVTFTEPTVLVIGAEGPGLSVSTQHHCDQMVSIPLQGKTASLNASVATGMALYEVYRQR
EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
WVETLHLHP
HHHHHCCCC
>Mature Secondary Structure 
TKARPSHNNHGKSKPRKPQRSYSSAKKPHRQSTSSRPPQKPRIRSKDKEPAQSEEEQGD
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LIYGRHSVLSALEHQRSLNRVWVVSRLRYDPRFHSLLNQAKSQGTIIDEVDHRRLNQLTQ
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QATHQGIAAQVAAYEYLDISTLIEQANAATPKPVIIAADSITDPHNLGAMIRTAEALGAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
GVIIPQRRAAGITSVVAKVATGALETLAIARVVNLNQGLEQLKKAGFWIYGLSTSAPQSI
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCE
HTVTFTEPTVLVIGAEGPGLSVSTQHHCDQMVSIPLQGKTASLNASVATGMALYEVYRQR
EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
WVETLHLHP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]