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The map label for this gene is vat [H]

Identifier: 158338278

GI number: 158338278

Start: 5238571

End: 5239191

Strand: Direct

Name: vat [H]

Synonym: AM1_5174

Alternate gene names: 158338278

Gene position: 5238571-5239191 (Clockwise)

Preceding gene: 158338277

Following gene: 158338279

Centisome position: 80.55

GC content: 47.18

Gene sequence:

>621_bases
ATGGAATACCCTGACCCTACCGTGGTTCATCCTATGGCGGGGTTTCCGCAAATTTGCTTTATCAAAAATACGGTCACCAA
TCCCAACATCGTGATTGGTGACTACACCTATTATGATGACCCCATTGACTCCGAGAACTTTGAGCGCAACGTCCTCTATC
ACTTCCCTTTTGTTGGTGACAAGCTGATCATTGGCAGGTTCTGCGCTTTATCCCGAGGCATTCGGTTCATCATGAATGGG
GCAAACCATAAAATGTCCGGCTTTTCTACCTATCCTTTTGAGATCTTTGGCAAGGATTGGCAGCGGATCAATGAGTATCC
CTACAAGGGAGATACCGTCATTGGTCATGATGTTTGGATTGGTTATGAGGCCGTGATCATGCCCGGAATCAAGATTGGCG
ATGGGGCCATCATCGCTGCTCAGTCTATGGTCACCAAAGAAGTTCCACCTTACACCATTGTGGGCGGTAATCCTGCCACT
GTAATTCGCCAGCGTTTTCCCGATACAGTCATTCAAACATTGCTAGAGATTGCCTGGTGGAATTGGGATATTGAAACCAT
CACAGCCAACCTGGAGAAAATTGTGGGTGCCGATATTGCTGCATTGCGAACATGTCGATAA

Upstream 100 bases:

>100_bases
GTAACTATGTCTCTGCCGAAGACAAAGCCTATTTAAAAGCTGGCAAATATCAAGTCAGTTATCTAGGCTATGATTGGTCC
CTCAATAAGCAATAAACTCC

Downstream 100 bases:

>100_bases
TTCTCTACCTTTTGGGCATTGCTAGAAGGAATGCCTTTAGCAGGGCATAAACGAATTAATGTCGATCGGGATTATCAGTA
TGGGGATTAATTTCGTTCCC

Product: acetyltransferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MEYPDPTVVHPMAGFPQICFIKNTVTNPNIVIGDYTYYDDPIDSENFERNVLYHFPFVGDKLIIGRFCALSRGIRFIMNG
ANHKMSGFSTYPFEIFGKDWQRINEYPYKGDTVIGHDVWIGYEAVIMPGIKIGDGAIIAAQSMVTKEVPPYTIVGGNPAT
VIRQRFPDTVIQTLLEIAWWNWDIETITANLEKIVGADIAALRTCR

Sequences:

>Translated_206_residues
MEYPDPTVVHPMAGFPQICFIKNTVTNPNIVIGDYTYYDDPIDSENFERNVLYHFPFVGDKLIIGRFCALSRGIRFIMNG
ANHKMSGFSTYPFEIFGKDWQRINEYPYKGDTVIGHDVWIGYEAVIMPGIKIGDGAIIAAQSMVTKEVPPYTIVGGNPAT
VIRQRFPDTVIQTLLEIAWWNWDIETITANLEKIVGADIAALRTCR
>Mature_206_residues
MEYPDPTVVHPMAGFPQICFIKNTVTNPNIVIGDYTYYDDPIDSENFERNVLYHFPFVGDKLIIGRFCALSRGIRFIMNG
ANHKMSGFSTYPFEIFGKDWQRINEYPYKGDTVIGHDVWIGYEAVIMPGIKIGDGAIIAAQSMVTKEVPPYTIVGGNPAT
VIRQRFPDTVIQTLLEIAWWNWDIETITANLEKIVGADIAALRTCR

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=51, Percent_Identity=52.9411764705882, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23285; Mature: 23285

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYPDPTVVHPMAGFPQICFIKNTVTNPNIVIGDYTYYDDPIDSENFERNVLYHFPFVGD
CCCCCCCEECCCCCCCEEEEEEECCCCCCEEECCCEECCCCCCCCCCCCCEEEECCCCCC
KLIIGRFCALSRGIRFIMNGANHKMSGFSTYPFEIFGKDWQRINEYPYKGDTVIGHDVWI
HHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHEECCHHHHHHCCCCCCCEEEECEEEE
GYEAVIMPGIKIGDGAIIAAQSMVTKEVPPYTIVGGNPATVIRQRFPDTVIQTLLEIAWW
CEEEEEECCEEECCCEEEEHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHC
NWDIETITANLEKIVGADIAALRTCR
CCCHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MEYPDPTVVHPMAGFPQICFIKNTVTNPNIVIGDYTYYDDPIDSENFERNVLYHFPFVGD
CCCCCCCEECCCCCCCEEEEEEECCCCCCEEECCCEECCCCCCCCCCCCCEEEECCCCCC
KLIIGRFCALSRGIRFIMNGANHKMSGFSTYPFEIFGKDWQRINEYPYKGDTVIGHDVWI
HHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHEECCHHHHHHCCCCCCCEEEECEEEE
GYEAVIMPGIKIGDGAIIAAQSMVTKEVPPYTIVGGNPATVIRQRFPDTVIQTLLEIAWW
CEEEEEECCEEECCCEEEEHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHC
NWDIETITANLEKIVGADIAALRTCR
CCCHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]