Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 158338268

GI number: 158338268

Start: 5227250

End: 5228545

Strand: Direct

Name: purB [H]

Synonym: AM1_5164

Alternate gene names: 158338268

Gene position: 5227250-5228545 (Clockwise)

Preceding gene: 158338267

Following gene: 158338274

Centisome position: 80.37

GC content: 52.24

Gene sequence:

>1296_bases
TTGATCGAGCGGTATACCTTGCCCAAAATGGGCGATCTGTGGACAGATGAGAATAAGTTCAGAACCTGGTTGCAGGTGGA
AATTGCCGTTTGCGAGGCCCAGGCAGAACTGGGAAAGATTCCTGCCGATGCCTTGGCCACGATCAAAGCCAAAGCCAATT
TCGACGTTAAGCGCATTCTAGAAATCGAAGCGGAAGTCAAGCATGATGTGATTGCGTTCTTGACCAACGTTAATGAGTAC
GTCGGTGAAGCTGGACGATATATCCATTTGGGCATGACCAGCTCTGATGTGTTGGATACGGCTATTGCCCTGCAGATGGT
GGCCAGCCTCGATATCCTGCTGCCCCAACTCGAAAATCTGATTCAAGCCATTCGTTACCAAGCCCAGCAGCATCGCCAAA
CCGTGATGGTGGGCCGTAGCCACGGTATTCATGCCGAGCCGATTACCCTGGGATTTAAGCTGGCAGGCTGGTTAGCTGAA
ATGATGCGACATCGCGATCGCCTGATCGCAGCGCAAAAGGAAGTCGCCGTCGGTCAAATCTCGGGTGCCGTTGGCACCTA
TGCCAATATTGACCCTCAGATTGAATGTCTCACCTGCCAAAAATTGGGGCTACAGCCAGAAGCCGCCTCCACCCAGGTAA
TTTCCCGAGATCGCCATGCCTACTATCTCAATGCCCTAGCTTTAGTGGCCGCCTCCATCGAACGATTTGCGGTAGAAATT
CGCAATCTTCAGCGCACGGATGTGCTGGAAGTGGAAGAATTCTTTTCCAAGGGGCAAAAAGGCTCTTCGGCCATGCCCCA
CAAGCGCAACCCGATTCGGTCAGAACGACTCACGGGACTGGCTCGGGTGGTACGAGGCTATGCCATGACAGGGCTTGAGA
ATGTGGCCCTCTGGCATGAACGGGATATTTCCCATAGCTCTGCAGAGCGGGTGATTGTCCCCGATGCCGCCATCTTGACC
CACTTTATGTTGGTGGAAATCACCGACCTAGTGAAACATCTCCAGGTCTATCCCGAGAATATGAAGCGGAATATGAACCT
GTATGGTGGCGTTATCTTTAGCCAGAGCGTTTTACTCGCCCTGGTGGATAAAGGCATGGTGCGCGAAGATGCCTATGCCA
TTGTCCAAACCCATGCTCACCAGGCTTGGAACAAAGAAGGCGGAAATTTTAAAGCCGCCCTCAGCCAAGACCCACAGGTG
CAAAAACTGCTCTCGGCTGAGGAACTAGACCACTGCTTTGACCCCAGTCGCCACTTACAAAACCTCGATCAGGTTTATCA
GCGATTGGATATCTAG

Upstream 100 bases:

>100_bases
CTGACCCTGTCTAATCTTGGCGTTCTGCCTTTGACGATTAAAAACCGTTAGAGAATAGCAAGGCAGCGTACAATAGTTCA
GTTGAAATCTAGGCAATACA

Downstream 100 bases:

>100_bases
GGGGCTACCACTTCTTATAGGGTAGGAACTTACCGCACATGGTTACCTGAACGCGATCTCCTTTTGGATCTGGCACTTTA
TCCACCTCAATGGAGAAATC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPKMGDLWTDENKFRTWLQVEIAVCEAQAELGKIPADALATIKAKANFDVKRILEIEAEVKHDVIAFLTNVNEY
VGEAGRYIHLGMTSSDVLDTAIALQMVASLDILLPQLENLIQAIRYQAQQHRQTVMVGRSHGIHAEPITLGFKLAGWLAE
MMRHRDRLIAAQKEVAVGQISGAVGTYANIDPQIECLTCQKLGLQPEAASTQVISRDRHAYYLNALALVAASIERFAVEI
RNLQRTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGLARVVRGYAMTGLENVALWHERDISHSSAERVIVPDAAILT
HFMLVEITDLVKHLQVYPENMKRNMNLYGGVIFSQSVLLALVDKGMVREDAYAIVQTHAHQAWNKEGGNFKAALSQDPQV
QKLLSAEELDHCFDPSRHLQNLDQVYQRLDI

Sequences:

>Translated_431_residues
MIERYTLPKMGDLWTDENKFRTWLQVEIAVCEAQAELGKIPADALATIKAKANFDVKRILEIEAEVKHDVIAFLTNVNEY
VGEAGRYIHLGMTSSDVLDTAIALQMVASLDILLPQLENLIQAIRYQAQQHRQTVMVGRSHGIHAEPITLGFKLAGWLAE
MMRHRDRLIAAQKEVAVGQISGAVGTYANIDPQIECLTCQKLGLQPEAASTQVISRDRHAYYLNALALVAASIERFAVEI
RNLQRTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGLARVVRGYAMTGLENVALWHERDISHSSAERVIVPDAAILT
HFMLVEITDLVKHLQVYPENMKRNMNLYGGVIFSQSVLLALVDKGMVREDAYAIVQTHAHQAWNKEGGNFKAALSQDPQV
QKLLSAEELDHCFDPSRHLQNLDQVYQRLDI
>Mature_431_residues
MIERYTLPKMGDLWTDENKFRTWLQVEIAVCEAQAELGKIPADALATIKAKANFDVKRILEIEAEVKHDVIAFLTNVNEY
VGEAGRYIHLGMTSSDVLDTAIALQMVASLDILLPQLENLIQAIRYQAQQHRQTVMVGRSHGIHAEPITLGFKLAGWLAE
MMRHRDRLIAAQKEVAVGQISGAVGTYANIDPQIECLTCQKLGLQPEAASTQVISRDRHAYYLNALALVAASIERFAVEI
RNLQRTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGLARVVRGYAMTGLENVALWHERDISHSSAERVIVPDAAILT
HFMLVEITDLVKHLQVYPENMKRNMNLYGGVIFSQSVLLALVDKGMVREDAYAIVQTHAHQAWNKEGGNFKAALSQDPQV
QKLLSAEELDHCFDPSRHLQNLDQVYQRLDI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:
GO:0003824: Adenylosuccinate lyase
GO:0004018: Adenylosuccinate lyase
GO:0009152: Adenylosuccinate lyase
GO:0016829: Adenylosuccinate lyase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=448, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1787376, Length=325, Percent_Identity=32, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787896, Length=210, Percent_Identity=30.4761904761905, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790398, Length=289, Percent_Identity=27.3356401384083, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=385, Percent_Identity=28.051948051948, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564234, Length=330, Percent_Identity=29.3939393939394, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=457, Percent_Identity=27.1334792122538, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324993, Length=206, Percent_Identity=28.1553398058252, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24647570, Length=430, Percent_Identity=26.046511627907, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48392; Mature: 48392

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPKMGDLWTDENKFRTWLQVEIAVCEAQAELGKIPADALATIKAKANFDVKRIL
CCCCCCCCCCCCCCCCCHHHHHHEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
EIEAEVKHDVIAFLTNVNEYVGEAGRYIHLGMTSSDVLDTAIALQMVASLDILLPQLENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAIRYQAQQHRQTVMVGRSHGIHAEPITLGFKLAGWLAEMMRHRDRLIAAQKEVAVGQI
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPQIECLTCQKLGLQPEAASTQVISRDRHAYYLNALALVAASIERFAVEI
CCCCCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGLARVVRGYAMTGLENVALWHE
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
RDISHSSAERVIVPDAAILTHFMLVEITDLVKHLQVYPENMKRNMNLYGGVIFSQSVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHH
LVDKGMVREDAYAIVQTHAHQAWNKEGGNFKAALSQDPQVQKLLSAEELDHCFDPSRHLQ
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHH
NLDQVYQRLDI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPKMGDLWTDENKFRTWLQVEIAVCEAQAELGKIPADALATIKAKANFDVKRIL
CCCCCCCCCCCCCCCCCHHHHHHEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
EIEAEVKHDVIAFLTNVNEYVGEAGRYIHLGMTSSDVLDTAIALQMVASLDILLPQLENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAIRYQAQQHRQTVMVGRSHGIHAEPITLGFKLAGWLAEMMRHRDRLIAAQKEVAVGQI
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPQIECLTCQKLGLQPEAASTQVISRDRHAYYLNALALVAASIERFAVEI
CCCCCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGLARVVRGYAMTGLENVALWHE
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
RDISHSSAERVIVPDAAILTHFMLVEITDLVKHLQVYPENMKRNMNLYGGVIFSQSVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHH
LVDKGMVREDAYAIVQTHAHQAWNKEGGNFKAALSQDPQVQKLLSAEELDHCFDPSRHLQ
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHH
NLDQVYQRLDI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]