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The map label for this gene is trpE [H]

Identifier: 158338247

GI number: 158338247

Start: 5204603

End: 5206138

Strand: Reverse

Name: trpE [H]

Synonym: AM1_5143

Alternate gene names: 158338247

Gene position: 5206138-5204603 (Counterclockwise)

Preceding gene: 158338248

Following gene: 158338246

Centisome position: 80.05

GC content: 49.93

Gene sequence:

>1536_bases
TTGCCTGACTTTAGTCAATTTGAAGCATTGGCCCGATCGGGCAATTTTATTCCTGTTTATCGAGAATGGATTGCGGATTT
AGATACGCCAGTCTCAGCTTGGTATCGGGTCTGTGCCGATCAACCCTACAGTTTCTTATTGGAATCCGTTGAGGGGGGAG
AACATCTGGCTCGATATAGCCTTTTGGGATGTGATCCAGTCTGGATTTTGGAAACTCGCGGTCAGCAAACGACCCAGACC
CATCGGAATGGATCGGTAGTGCCTTTTCCCGGTAACCCGTTTCAAATTCTCCAAGATTGTTTAGCGCCCTATCACCCCGT
AACCCTTCCTCAGCTTCCTGCTGGAATTGGCGGTCTGGTTGGCTTCTGGGGATACGAACTCATTAATTGGATTGAACCCC
GGGTACCGATCTATCCTGCAGCTAGCTCAGACTTACCGGATGGGCTGTGGATGCAGGTGGATAATTTAATGGTTTTCGAT
CAGGTCCAGCGCAAAATTTGGGTTATTGCCTATGCGGACTTGCAACAAACGCCTGATTTAAAGACTGCTTATCAGCAAGC
TTGCGATCGCGTTGAGTATCTGGTCCAAAAGCTACAACAGCCCCTCGATCGGCAAAGCTTGGAGCTGACGGGGATGACGC
CCACTCAGTCCGTTACGGCGGACTTTACAAGCAATACGACCCAAGCTGGATTCTGTCACAATGTTCAGCAGGCTAAAGAC
TATATCAAGGCTGGCGATATTTTCCAGGTGGTCTTATCCCAGCGGTTATCTACAGCCTATCAGGGCGACCCGTTTGAGCT
TTACCGATCCTTACGTTTGATCAATCCATCGCCCTATATGTCCTACCTGCACTTTAAAGATTGGCAAATTATTGGTTCCA
GTCCTGAAGTGATGGTGAAAGCGGAACATAGCACCCAGGAGAAGGGAGGATTGGCTGTAACCGTGCGACCCATCGCTGGG
ACTCGTCCCCGCGGTCGAACCTCCCAAGAAGATATGGCTTTAGCGACAGAACTTCTCCAGGATCCAAAAGAAATCGCAGA
GCATGTCATGCTCGTAGATTTAGGACGGAACGACTTGGGGCGAGTCTGTAGCAAAGGGTCGGTTCAAGTGAAGTCAGCAG
ATCTCATGGTGATTGAACGCTACTCCCATGTCATGCACATCGTGAGCAATGTTGTGGGAGAACTTGCGGATGACAAAACC
GCCTGGGATTTGCTCAAGGCCTGTTTTCCGGCCGGTACTGTCAGTGGTGCACCCAAGATCCGGGCCATGGAAATTATCCA
TGAGCTAGAAGGATGTCGGCGGGGTCCTTATTCAGGGGTGTATGGCTACTACGATTTTGAGGGACAGCTGAATACAGCCA
TTTCGATCCGCACCATGGTTGTCCAACAACAAACCAAGGGTCAACATCTAGTAACAGTGCAGGCAGGGGCAGGAATTGTA
GCGGATTCTGAACCTATTCGAGAATATGAAGAAACCCTCAATAAGGCTAGGGGACTGTTACAAGCCATTCATTGCGTACA
GGGTGCGTCCACCTGA

Upstream 100 bases:

>100_bases
ACCCCTCCATTGGTGGGGTTCAACTTTTTTTTGATATTGTCTAGCCATTAACGTGTTTTTGGCCATTTCTCTCAATCAAT
AGGTTGTTGCAGTGACAGTT

Downstream 100 bases:

>100_bases
TCATGACAACCTACCAAGTCCGATTTATCAACCCTGACTTAGGACTGGATCAAACGATCACCATTCCAGAGGATGAATAC
ATTCTCGATATTGCCGAAGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPDFSQFEALARSGNFIPVYREWIADLDTPVSAWYRVCADQPYSFLLESVEGGEHLARYSLLGCDPVWILETRGQQTTQT
HRNGSVVPFPGNPFQILQDCLAPYHPVTLPQLPAGIGGLVGFWGYELINWIEPRVPIYPAASSDLPDGLWMQVDNLMVFD
QVQRKIWVIAYADLQQTPDLKTAYQQACDRVEYLVQKLQQPLDRQSLELTGMTPTQSVTADFTSNTTQAGFCHNVQQAKD
YIKAGDIFQVVLSQRLSTAYQGDPFELYRSLRLINPSPYMSYLHFKDWQIIGSSPEVMVKAEHSTQEKGGLAVTVRPIAG
TRPRGRTSQEDMALATELLQDPKEIAEHVMLVDLGRNDLGRVCSKGSVQVKSADLMVIERYSHVMHIVSNVVGELADDKT
AWDLLKACFPAGTVSGAPKIRAMEIIHELEGCRRGPYSGVYGYYDFEGQLNTAISIRTMVVQQQTKGQHLVTVQAGAGIV
ADSEPIREYEETLNKARGLLQAIHCVQGAST

Sequences:

>Translated_511_residues
MPDFSQFEALARSGNFIPVYREWIADLDTPVSAWYRVCADQPYSFLLESVEGGEHLARYSLLGCDPVWILETRGQQTTQT
HRNGSVVPFPGNPFQILQDCLAPYHPVTLPQLPAGIGGLVGFWGYELINWIEPRVPIYPAASSDLPDGLWMQVDNLMVFD
QVQRKIWVIAYADLQQTPDLKTAYQQACDRVEYLVQKLQQPLDRQSLELTGMTPTQSVTADFTSNTTQAGFCHNVQQAKD
YIKAGDIFQVVLSQRLSTAYQGDPFELYRSLRLINPSPYMSYLHFKDWQIIGSSPEVMVKAEHSTQEKGGLAVTVRPIAG
TRPRGRTSQEDMALATELLQDPKEIAEHVMLVDLGRNDLGRVCSKGSVQVKSADLMVIERYSHVMHIVSNVVGELADDKT
AWDLLKACFPAGTVSGAPKIRAMEIIHELEGCRRGPYSGVYGYYDFEGQLNTAISIRTMVVQQQTKGQHLVTVQAGAGIV
ADSEPIREYEETLNKARGLLQAIHCVQGAST
>Mature_510_residues
PDFSQFEALARSGNFIPVYREWIADLDTPVSAWYRVCADQPYSFLLESVEGGEHLARYSLLGCDPVWILETRGQQTTQTH
RNGSVVPFPGNPFQILQDCLAPYHPVTLPQLPAGIGGLVGFWGYELINWIEPRVPIYPAASSDLPDGLWMQVDNLMVFDQ
VQRKIWVIAYADLQQTPDLKTAYQQACDRVEYLVQKLQQPLDRQSLELTGMTPTQSVTADFTSNTTQAGFCHNVQQAKDY
IKAGDIFQVVLSQRLSTAYQGDPFELYRSLRLINPSPYMSYLHFKDWQIIGSSPEVMVKAEHSTQEKGGLAVTVRPIAGT
RPRGRTSQEDMALATELLQDPKEIAEHVMLVDLGRNDLGRVCSKGSVQVKSADLMVIERYSHVMHIVSNVVGELADDKTA
WDLLKACFPAGTVSGAPKIRAMEIIHELEGCRRGPYSGVYGYYDFEGQLNTAISIRTMVVQQQTKGQHLVTVQAGAGIVA
DSEPIREYEETLNKARGLLQAIHCVQGAST

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:
GO:0000162: Anthranilate synthase component I
GO:0004049: Anthranilate synthase component I
GO:0009058: Anthranilate synthase component I
GO:0016833: Anthranilate synthase component I

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=527, Percent_Identity=33.2068311195446, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788114, Length=466, Percent_Identity=31.9742489270386, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082077, Length=280, Percent_Identity=25.7142857142857, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786809, Length=277, Percent_Identity=25.6317689530686, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=506, Percent_Identity=36.7588932806324, Blast_Score=300, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6324361, Length=415, Percent_Identity=27.710843373494, Blast_Score=120, Evalue=6e-28,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 56930; Mature: 56799

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFSQFEALARSGNFIPVYREWIADLDTPVSAWYRVCADQPYSFLLESVEGGEHLARYS
CCCHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
LLGCDPVWILETRGQQTTQTHRNGSVVPFPGNPFQILQDCLAPYHPVTLPQLPAGIGGLV
HCCCCEEEEEECCCCHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHH
GFWGYELINWIEPRVPIYPAASSDLPDGLWMQVDNLMVFDQVQRKIWVIAYADLQQTPDL
HHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHCCHHHHHHCCEEEEEEEECCCCCCCH
KTAYQQACDRVEYLVQKLQQPLDRQSLELTGMTPTQSVTADFTSNTTQAGFCHNVQQAKD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCHHHCCHHHHHH
YIKAGDIFQVVLSQRLSTAYQGDPFELYRSLRLINPSPYMSYLHFKDWQIIGSSPEVMVK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHEECCCEEEECCCCCEEEE
AEHSTQEKGGLAVTVRPIAGTRPRGRTSQEDMALATELLQDPKEIAEHVMLVDLGRNDLG
ECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHEEEEECCCCHHH
RVCSKGSVQVKSADLMVIERYSHVMHIVSNVVGELADDKTAWDLLKACFPAGTVSGAPKI
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCH
RAMEIIHELEGCRRGPYSGVYGYYDFEGQLNTAISIRTMVVQQQTKGQHLVTVQAGAGIV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCCEE
ADSEPIREYEETLNKARGLLQAIHCVQGAST
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PDFSQFEALARSGNFIPVYREWIADLDTPVSAWYRVCADQPYSFLLESVEGGEHLARYS
CCHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
LLGCDPVWILETRGQQTTQTHRNGSVVPFPGNPFQILQDCLAPYHPVTLPQLPAGIGGLV
HCCCCEEEEEECCCCHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHH
GFWGYELINWIEPRVPIYPAASSDLPDGLWMQVDNLMVFDQVQRKIWVIAYADLQQTPDL
HHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHCCHHHHHHCCEEEEEEEECCCCCCCH
KTAYQQACDRVEYLVQKLQQPLDRQSLELTGMTPTQSVTADFTSNTTQAGFCHNVQQAKD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCHHHCCHHHHHH
YIKAGDIFQVVLSQRLSTAYQGDPFELYRSLRLINPSPYMSYLHFKDWQIIGSSPEVMVK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHEECCCEEEECCCCCEEEE
AEHSTQEKGGLAVTVRPIAGTRPRGRTSQEDMALATELLQDPKEIAEHVMLVDLGRNDLG
ECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHEEEEECCCCHHH
RVCSKGSVQVKSADLMVIERYSHVMHIVSNVVGELADDKTAWDLLKACFPAGTVSGAPKI
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCH
RAMEIIHELEGCRRGPYSGVYGYYDFEGQLNTAISIRTMVVQQQTKGQHLVTVQAGAGIV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCCEE
ADSEPIREYEETLNKARGLLQAIHCVQGAST
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]