Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 158338233

GI number: 158338233

Start: 5187556

End: 5189013

Strand: Reverse

Name: gltX

Synonym: AM1_5128

Alternate gene names: 158338233

Gene position: 5189013-5187556 (Counterclockwise)

Preceding gene: 158338234

Following gene: 158338230

Centisome position: 79.79

GC content: 51.03

Gene sequence:

>1458_bases
GTGACCGTTCGAGTTCGTATCGCCCCTAGTCCAACGGGGAACCTTCATATTGGCACTGCTAGAACTGCAGTCTTTAATTG
GCTATATGCTCGCCATCACCAGGGCACCTTCATCCTGCGGGTTGAAGATACGGATGAGGAGCGATCGCAACCGGAATACA
CCGAGAATATTCTGGAAGGATTGCGCTGGCTAGGAATGGAGTGGGATGAAGGTCCTTATTTCCAGTCGGAACGGTTGGAT
TTGTATCGGGAGGCGATTCAAAAGCTGCTCGATCAGGGCCTTGCCTATCGCTGCTACTGTACGCCTGAAGAGCTGGATGA
GATGCGAACGGCTCAAAAAGAGCGGAATGAAGCCCCCCGATACGACAATCGCCATCGCAATTTAACAGCGGAGCAAGAAG
CTGCTTTCCAAGCCGAAGGTCGTCAGCCGGTGATCCGCTTCAAGATTGAGGATGACCAAACCATTACCTGGAAGGACTTG
GTTCGCGGTCCCATGCATTGGCAAGGACGAGATTTGGGGGGCGATATGGTGGTTGCGAGAGCCACCGCTTCTGGGGAAGT
GGGCCAACCCCTCTACAACTTGGCAGTAGTTGTAGACGATATGGATTTGAACATCTCCCATGTAATCCGAGGGGAAGACC
ATATTGCTAATACGGCCAAGCAGATTTTGCTTTATCAAGCCTTTGGCGGTACGGTACCTGAATTTGCTCATACTCCCTTA
ATCCTGAATAAGGAGGGGCGGAAGCTGTCGAAACGGGATGGCGTGACGTCTATTTCTGATTTTCAGAAGATGGGATTTAC
GGCCCCAGCCTTGGCGAACTATATGACCCTATTGGGCTGGTCTCCGCCTGATGCGACCCAGGAAATTTTCAGCTTGAGCG
ATGCGGCGGCTCAATTTGATTTTGAGCGGGTGAATAAGGCGGGGGCTAAGTTTGATTGGGACAAGTTGGATTGGATCAGT
AGTCAATATCTTCATGGGCTGCCTTTGGCGGAGTTAGCGGATGCGTTGATGCCCTATTGGCAAGCTGCGAAATATGACTT
TGACCCGGTGGCCGATCGGCCTTGGCTCGACCAAGTGACGGCCTTGATTCAGCCGAGTTTGGTCAGACTGAAGGATGTTG
TCGAGATGACGAAGTTTCTCTTTACCCGTGAATTGACCTGGAGTGAGGATGCTCAGAAGCAGTTGAGCCAAGAGAGCGTA
GCGGTAGCATTACAAGGGGTGATTGACCCGCTGGCGGCGGCTGCAGAATCGGAGTTTTCAGCAGAAATCGCAAAATCGTT
TATTAATTCGACGGTGAAAGCCAACAAGCTGAAGAAGGGATTGGTAATGCGATCTCTTCGTGCTGCATTAACTGGAGATA
TGAAGGGTCCCGATTTGATGGAGTCTTGGTTATTACTCCATCAGCGGGGAGAAGATCTCAAACGCCTACAACAAGCGTTA
GAAATCGCGAGTGCATAA

Upstream 100 bases:

>100_bases
TGTCCCATATTTCTGTATGAAGCCACCTGGGAGATTTTCGGATCTCTATTCGGGTGGCTTTATCGGTAGGATGGAATCTG
TAATGTAACCGAGAGATTTA

Downstream 100 bases:

>100_bases
GCTAATGGCTGAAGGCAGCAACGACTCGATCTTCTATTTCTGGATCTAGTAAGACCATGGCACTGGCGGAGTTCTGGATA
TCTGTGGGTGGGTTTTGACG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTVRVRIAPSPTGNLHIGTARTAVFNWLYARHHQGTFILRVEDTDEERSQPEYTENILEGLRWLGMEWDEGPYFQSERLD
LYREAIQKLLDQGLAYRCYCTPEELDEMRTAQKERNEAPRYDNRHRNLTAEQEAAFQAEGRQPVIRFKIEDDQTITWKDL
VRGPMHWQGRDLGGDMVVARATASGEVGQPLYNLAVVVDDMDLNISHVIRGEDHIANTAKQILLYQAFGGTVPEFAHTPL
ILNKEGRKLSKRDGVTSISDFQKMGFTAPALANYMTLLGWSPPDATQEIFSLSDAAAQFDFERVNKAGAKFDWDKLDWIS
SQYLHGLPLAELADALMPYWQAAKYDFDPVADRPWLDQVTALIQPSLVRLKDVVEMTKFLFTRELTWSEDAQKQLSQESV
AVALQGVIDPLAAAAESEFSAEIAKSFINSTVKANKLKKGLVMRSLRAALTGDMKGPDLMESWLLLHQRGEDLKRLQQAL
EIASA

Sequences:

>Translated_485_residues
MTVRVRIAPSPTGNLHIGTARTAVFNWLYARHHQGTFILRVEDTDEERSQPEYTENILEGLRWLGMEWDEGPYFQSERLD
LYREAIQKLLDQGLAYRCYCTPEELDEMRTAQKERNEAPRYDNRHRNLTAEQEAAFQAEGRQPVIRFKIEDDQTITWKDL
VRGPMHWQGRDLGGDMVVARATASGEVGQPLYNLAVVVDDMDLNISHVIRGEDHIANTAKQILLYQAFGGTVPEFAHTPL
ILNKEGRKLSKRDGVTSISDFQKMGFTAPALANYMTLLGWSPPDATQEIFSLSDAAAQFDFERVNKAGAKFDWDKLDWIS
SQYLHGLPLAELADALMPYWQAAKYDFDPVADRPWLDQVTALIQPSLVRLKDVVEMTKFLFTRELTWSEDAQKQLSQESV
AVALQGVIDPLAAAAESEFSAEIAKSFINSTVKANKLKKGLVMRSLRAALTGDMKGPDLMESWLLLHQRGEDLKRLQQAL
EIASA
>Mature_484_residues
TVRVRIAPSPTGNLHIGTARTAVFNWLYARHHQGTFILRVEDTDEERSQPEYTENILEGLRWLGMEWDEGPYFQSERLDL
YREAIQKLLDQGLAYRCYCTPEELDEMRTAQKERNEAPRYDNRHRNLTAEQEAAFQAEGRQPVIRFKIEDDQTITWKDLV
RGPMHWQGRDLGGDMVVARATASGEVGQPLYNLAVVVDDMDLNISHVIRGEDHIANTAKQILLYQAFGGTVPEFAHTPLI
LNKEGRKLSKRDGVTSISDFQKMGFTAPALANYMTLLGWSPPDATQEIFSLSDAAAQFDFERVNKAGAKFDWDKLDWISS
QYLHGLPLAELADALMPYWQAAKYDFDPVADRPWLDQVTALIQPSLVRLKDVVEMTKFLFTRELTWSEDAQKQLSQESVA
VALQGVIDPLAAAAESEFSAEIAKSFINSTVKANKLKKGLVMRSLRAALTGDMKGPDLMESWLLLHQRGEDLKRLQQALE
IASA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:
GO:0000166: Glutamyl-tRNA synthetase
GO:0004812: Glutamyl-tRNA synthetase
GO:0004818: Glutamyl-tRNA synthetase
GO:0005524: Glutamyl-tRNA synthetase
GO:0005737: Glutamyl-tRNA synthetase
GO:0006412: Glutamyl-tRNA synthetase
GO:0006418: Glutamyl-tRNA synthetase
GO:0006424: Glutamyl-tRNA synthetase
GO:0016874: Glutamyl-tRNA synthetase
GO:0016876: Glutamyl-tRNA synthetase
GO:0043039: Glutamyl-tRNA synthetase

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=505, Percent_Identity=35.6435643564356, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI4826960, Length=112, Percent_Identity=37.5, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788743, Length=484, Percent_Identity=36.7768595041322, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI226510925, Length=275, Percent_Identity=35.6363636363636, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786895, Length=340, Percent_Identity=25, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=33.2631578947368, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=493, Percent_Identity=32.2515212981744, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=131, Percent_Identity=38.1679389312977, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=26.8085106382979, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=36.8, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17864488, Length=103, Percent_Identity=36.8932038834951, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ACAM1 (B0C8D6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519410.1
- ProteinModelPortal:   B0C8D6
- SMR:   B0C8D6
- GeneID:   5683924
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5128
- HOGENOM:   HBG628189
- OMA:   DEASWTK
- ProtClustDB:   PRK01406
- BioCyc:   AMAR329726:AM1_5128-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54890; Mature: 54759

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVRIAPSPTGNLHIGTARTAVFNWLYARHHQGTFILRVEDTDEERSQPEYTENILEG
CEEEEEECCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LRWLGMEWDEGPYFQSERLDLYREAIQKLLDQGLAYRCYCTPEELDEMRTAQKERNEAPR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCC
YDNRHRNLTAEQEAAFQAEGRQPVIRFKIEDDQTITWKDLVRGPMHWQGRDLGGDMVVAR
CCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEE
ATASGEVGQPLYNLAVVVDDMDLNISHVIRGEDHIANTAKQILLYQAFGGTVPEFAHTPL
ECCCCCCCCCCEEEEEEEECCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHCCCE
ILNKEGRKLSKRDGVTSISDFQKMGFTAPALANYMTLLGWSPPDATQEIFSLSDAAAQFD
EECCCCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FERVNKAGAKFDWDKLDWISSQYLHGLPLAELADALMPYWQAAKYDFDPVADRPWLDQVT
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ALIQPSLVRLKDVVEMTKFLFTRELTWSEDAQKQLSQESVAVALQGVIDPLAAAAESEFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIAKSFINSTVKANKLKKGLVMRSLRAALTGDMKGPDLMESWLLLHQRGEDLKRLQQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
EIASA
HHHCC
>Mature Secondary Structure 
TVRVRIAPSPTGNLHIGTARTAVFNWLYARHHQGTFILRVEDTDEERSQPEYTENILEG
EEEEEECCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LRWLGMEWDEGPYFQSERLDLYREAIQKLLDQGLAYRCYCTPEELDEMRTAQKERNEAPR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCC
YDNRHRNLTAEQEAAFQAEGRQPVIRFKIEDDQTITWKDLVRGPMHWQGRDLGGDMVVAR
CCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEE
ATASGEVGQPLYNLAVVVDDMDLNISHVIRGEDHIANTAKQILLYQAFGGTVPEFAHTPL
ECCCCCCCCCCEEEEEEEECCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHCCCE
ILNKEGRKLSKRDGVTSISDFQKMGFTAPALANYMTLLGWSPPDATQEIFSLSDAAAQFD
EECCCCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FERVNKAGAKFDWDKLDWISSQYLHGLPLAELADALMPYWQAAKYDFDPVADRPWLDQVT
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ALIQPSLVRLKDVVEMTKFLFTRELTWSEDAQKQLSQESVAVALQGVIDPLAAAAESEFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIAKSFINSTVKANKLKKGLVMRSLRAALTGDMKGPDLMESWLLLHQRGEDLKRLQQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
EIASA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA