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The map label for this gene is vraR [H]

Identifier: 158338175

GI number: 158338175

Start: 5119472

End: 5120146

Strand: Direct

Name: vraR [H]

Synonym: AM1_5067

Alternate gene names: 158338175

Gene position: 5119472-5120146 (Clockwise)

Preceding gene: 158338172

Following gene: 158338176

Centisome position: 78.72

GC content: 52.0

Gene sequence:

>675_bases
ATGATGACGCCTCAGAATCCAATTCGAGTATTGCTAGTGGATGATGAGGACATCGTTCGCTACGGGTTAAAAGCGATCGC
GCAAGGCACACCTGAGATCGAAATTGTGGGTGAGGCTGGGTCGGGTAAAGCTGCGATCGCACAGGCCAAAACGCTACAAC
CCGATGTGATTCTGATGGACATCAACATGCCCCAGATGGATGGTGTGACGGCCACCCAAGAGATTAGTCGCATTCAGCCC
CATGCTAAAGTCCTCATCTTAACCACCCATGATGAAGCTGAGTATCTAGTGGAAGCGATGCAGCAAGGCGCTGTCGGTTA
CCTACTCAAAAATACACCCCCAGAAGACTTCATCCAAAGCATCCAGCAGGCACACAAGGGATATCTACAGCTCGGTCCTG
GCCTAGGTCAAAAGCTCTGTCAACTTCTGAAAACCCCAGTGCGGCCTCAAATTGAAGCCTTCGAGGGCATCACCCCCAGA
GAACAGGACGTCTTGAAGCTGGTTGCGACAGGGGCGAGTAACCGCGAAATTTCTGAGACGCTCCACATCACCGAAAAAAC
GGTTAAAAATCACGTCAGTAGCATTTTGAGCCGAGTGGGTGTACAGTCCCGCACCCAGCTTGCTATCTGGGCTAATTCCG
CTGGTGAAGAGACCTCTTCCTTTGCCGTGGCCTAG

Upstream 100 bases:

>100_bases
CTCTCATCCAGGGCTAGGAAACGCCTACTAAAATCCTTAGGTAGAGAGAGAAATACTGTTTTGATCTTTTGTTCAAAAGT
GTGGAGCTAGTCGCACATTT

Downstream 100 bases:

>100_bases
TAGAATTATCAATTAGACAGACTCTGAGGGGGATTGCATTACGAACAATGCCTTGGAAGCTCGGTGTCAAATTTAGTGTG
TTCTTGGTGCTGATTTTCTT

Product: two-component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MMTPQNPIRVLLVDDEDIVRYGLKAIAQGTPEIEIVGEAGSGKAAIAQAKTLQPDVILMDINMPQMDGVTATQEISRIQP
HAKVLILTTHDEAEYLVEAMQQGAVGYLLKNTPPEDFIQSIQQAHKGYLQLGPGLGQKLCQLLKTPVRPQIEAFEGITPR
EQDVLKLVATGASNREISETLHITEKTVKNHVSSILSRVGVQSRTQLAIWANSAGEETSSFAVA

Sequences:

>Translated_224_residues
MMTPQNPIRVLLVDDEDIVRYGLKAIAQGTPEIEIVGEAGSGKAAIAQAKTLQPDVILMDINMPQMDGVTATQEISRIQP
HAKVLILTTHDEAEYLVEAMQQGAVGYLLKNTPPEDFIQSIQQAHKGYLQLGPGLGQKLCQLLKTPVRPQIEAFEGITPR
EQDVLKLVATGASNREISETLHITEKTVKNHVSSILSRVGVQSRTQLAIWANSAGEETSSFAVA
>Mature_224_residues
MMTPQNPIRVLLVDDEDIVRYGLKAIAQGTPEIEIVGEAGSGKAAIAQAKTLQPDVILMDINMPQMDGVTATQEISRIQP
HAKVLILTTHDEAEYLVEAMQQGAVGYLLKNTPPEDFIQSIQQAHKGYLQLGPGLGQKLCQLLKTPVRPQIEAFEGITPR
EQDVLKLVATGASNREISETLHITEKTVKNHVSSILSRVGVQSRTQLAIWANSAGEETSSFAVA

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787473, Length=211, Percent_Identity=36.0189573459716, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=34.4497607655502, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788222, Length=216, Percent_Identity=31.9444444444444, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=29.2682926829268, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=28.8557213930348, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788712, Length=180, Percent_Identity=22.7777777777778, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788550, Length=103, Percent_Identity=39.8058252427184, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082052, Length=105, Percent_Identity=36.1904761904762, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788546, Length=211, Percent_Identity=25.1184834123223, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=37.0689655172414, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789937, Length=209, Percent_Identity=25.8373205741627, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=119, Percent_Identity=32.7731092436975, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24329; Mature: 24329

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTPQNPIRVLLVDDEDIVRYGLKAIAQGTPEIEIVGEAGSGKAAIAQAKTLQPDVILMD
CCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEE
INMPQMDGVTATQEISRIQPHAKVLILTTHDEAEYLVEAMQQGAVGYLLKNTPPEDFIQS
CCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHH
IQQAHKGYLQLGPGLGQKLCQLLKTPVRPQIEAFEGITPREQDVLKLVATGASNREISET
HHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
LHITEKTVKNHVSSILSRVGVQSRTQLAIWANSAGEETSSFAVA
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MMTPQNPIRVLLVDDEDIVRYGLKAIAQGTPEIEIVGEAGSGKAAIAQAKTLQPDVILMD
CCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEE
INMPQMDGVTATQEISRIQPHAKVLILTTHDEAEYLVEAMQQGAVGYLLKNTPPEDFIQS
CCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHH
IQQAHKGYLQLGPGLGQKLCQLLKTPVRPQIEAFEGITPREQDVLKLVATGASNREISET
HHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
LHITEKTVKNHVSSILSRVGVQSRTQLAIWANSAGEETSSFAVA
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]