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The map label for this gene is ada [H]

Identifier: 158338101

GI number: 158338101

Start: 5041648

End: 5042502

Strand: Direct

Name: ada [H]

Synonym: AM1_4991

Alternate gene names: 158338101

Gene position: 5041648-5042502 (Clockwise)

Preceding gene: 158338100

Following gene: 158338102

Centisome position: 77.52

GC content: 52.87

Gene sequence:

>855_bases
ATGCCTCCTCCAAATACCATTGCCAGCGAGATAGACACCTACGATCGCATCTCCGATGCCATTACTTTTATTCGCCAGCA
TCACCTAGAGCAGCCCAATCTTTCAACCATCGCCCAGCACGTCAACCTCAGCGACTATCACTTTCAGCGATTATTCACCC
AATGGGCAGGGATTAGCCCCAAACGCTTTCTCCAATACCTGACTCTAGACTATGCAAAATCGAGAATCGATCAGTCCCAG
AATCTGCTAGCCCTAACCGCCGAGTCAGGACTATCCAGCCCAGGACGACTCCACGACTTATTCATCACCCTAGAAGCCAT
GTCTCCCGGTGAATACCGAGCAGGAGGGGCTGGGCTCAATATCCGCTACGGTATCCACGAAACCCCCTTTGGATATTGCC
TAATTGCCCTTACCCCCCGTGGTATTTGCAACATTCATTTTTTGCCCAGTGCCGACGAACAAGCGAGCCAAAAGCAGCTC
CAAACAGACTGGCCCCAAGCCAAAATCATCCGTGATCAAACCGCAACGGGGAACGTGTGCGATCAAGTCTTCGCCGCCTC
TCCTTCCTCCCCCTTAGCCCTCCACCTCAAAGGCACCAACTTTCAGATTCAAGTCTGGCAAGCCCTCTTGAGAATTCCCC
AAGGAGAACTGACCACCTATCAAACCTTAGCCACCGCCATGGGTAAACCCACCGCCGCTCGCGCCATTGGTAATGCCGTA
GGCCGTAATCCCATCGCTTATCTGATTCCCTGCCATCGGGTGATTCGGAGAACAGGAGAATTGGGAGGCTATCGTTGGGG
ATGCGATCGAAAAGCAGCCATGCTGGGTTGGGAAGCCAGCCAAATCAAGGAGTAA

Upstream 100 bases:

>100_bases
TTAGAGAATGGAAAAAGTGTTGCCAGTTGCGCAAGAAACGGGTGGTTTTTACCGCTCAAGATCGATACCGTAGACGGAGA
AAACTGACTGACTCAACACC

Downstream 100 bases:

>100_bases
CTCTGCAAAATCTTGACCTAGAGATCAGCGCTGATCATGCCAAAATTTATGGTGATCGAAACCTTCAAGCCTAATTGCCG
AGACCTCGCCTACGAAAGAT

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPPPNTIASEIDTYDRISDAITFIRQHHLEQPNLSTIAQHVNLSDYHFQRLFTQWAGISPKRFLQYLTLDYAKSRIDQSQ
NLLALTAESGLSSPGRLHDLFITLEAMSPGEYRAGGAGLNIRYGIHETPFGYCLIALTPRGICNIHFLPSADEQASQKQL
QTDWPQAKIIRDQTATGNVCDQVFAASPSSPLALHLKGTNFQIQVWQALLRIPQGELTTYQTLATAMGKPTAARAIGNAV
GRNPIAYLIPCHRVIRRTGELGGYRWGCDRKAAMLGWEASQIKE

Sequences:

>Translated_284_residues
MPPPNTIASEIDTYDRISDAITFIRQHHLEQPNLSTIAQHVNLSDYHFQRLFTQWAGISPKRFLQYLTLDYAKSRIDQSQ
NLLALTAESGLSSPGRLHDLFITLEAMSPGEYRAGGAGLNIRYGIHETPFGYCLIALTPRGICNIHFLPSADEQASQKQL
QTDWPQAKIIRDQTATGNVCDQVFAASPSSPLALHLKGTNFQIQVWQALLRIPQGELTTYQTLATAMGKPTAARAIGNAV
GRNPIAYLIPCHRVIRRTGELGGYRWGCDRKAAMLGWEASQIKE
>Mature_283_residues
PPPNTIASEIDTYDRISDAITFIRQHHLEQPNLSTIAQHVNLSDYHFQRLFTQWAGISPKRFLQYLTLDYAKSRIDQSQN
LLALTAESGLSSPGRLHDLFITLEAMSPGEYRAGGAGLNIRYGIHETPFGYCLIALTPRGICNIHFLPSADEQASQKQLQ
TDWPQAKIIRDQTATGNVCDQVFAASPSSPLALHLKGTNFQIQVWQALLRIPQGELTTYQTLATAMGKPTAARAIGNAVG
RNPIAYLIPCHRVIRRTGELGGYRWGCDRKAAMLGWEASQIKE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=84, Percent_Identity=41.6666666666667, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788542, Length=287, Percent_Identity=33.4494773519164, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=50, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=91, Percent_Identity=43.956043956044, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=91, Percent_Identity=43.956043956044, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=136, Percent_Identity=34.5588235294118, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137554, Length=174, Percent_Identity=35.0574712643678, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31542; Mature: 31411

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPNTIASEIDTYDRISDAITFIRQHHLEQPNLSTIAQHVNLSDYHFQRLFTQWAGISP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCH
KRFLQYLTLDYAKSRIDQSQNLLALTAESGLSSPGRLHDLFITLEAMSPGEYRAGGAGLN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
IRYGIHETPFGYCLIALTPRGICNIHFLPSADEQASQKQLQTDWPQAKIIRDQTATGNVC
EEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHH
DQVFAASPSSPLALHLKGTNFQIQVWQALLRIPQGELTTYQTLATAMGKPTAARAIGNAV
HHHHHCCCCCCEEEEEECCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
GRNPIAYLIPCHRVIRRTGELGGYRWGCDRKAAMLGWEASQIKE
CCCCEEEEHHHHHHHHHHHCCCCEEECCCCCHHHCCCCHHHHCC
>Mature Secondary Structure 
PPPNTIASEIDTYDRISDAITFIRQHHLEQPNLSTIAQHVNLSDYHFQRLFTQWAGISP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCH
KRFLQYLTLDYAKSRIDQSQNLLALTAESGLSSPGRLHDLFITLEAMSPGEYRAGGAGLN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
IRYGIHETPFGYCLIALTPRGICNIHFLPSADEQASQKQLQTDWPQAKIIRDQTATGNVC
EEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHH
DQVFAASPSSPLALHLKGTNFQIQVWQALLRIPQGELTTYQTLATAMGKPTAARAIGNAV
HHHHHCCCCCCEEEEEECCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
GRNPIAYLIPCHRVIRRTGELGGYRWGCDRKAAMLGWEASQIKE
CCCCEEEEHHHHHHHHHHHCCCCEEECCCCCHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]