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The map label for this gene is fliA [H]

Identifier: 158338094

GI number: 158338094

Start: 5031055

End: 5031855

Strand: Reverse

Name: fliA [H]

Synonym: AM1_4984

Alternate gene names: 158338094

Gene position: 5031855-5031055 (Counterclockwise)

Preceding gene: 158338095

Following gene: 158338093

Centisome position: 77.37

GC content: 53.31

Gene sequence:

>801_bases
ATGGCTTATCAAACTTCTCTTCAATCGCAAACCATGGAACTTCTGGTCGCTTATCGCCAAAAGCCTTCCGTTCAGCTCAG
AAACCGCCTCGTGCGATTGAATATGGGTCTCGTTCGCAAGGTCGCTCACCGTCTGACCCACCAGTGCGCTGAGCCCTACG
AAGATCTAGAGCAATGCGGATTTCTAGGTTTGATTACTGCCATCGAGCGCTTTGACCCCTCCCAAGGATATGCCTTCAGT
TCCTTCGCAGTGCCTTACATTCGCGGCGAAATCCTCCACTTCCTTCGCGACCGTGCTAACACCGTTCGCATCCCCCGCCG
CTGGCAACAGCTCAGCCGGGATGCTGCCAAAGCCAGACAAGCCCTTACCATGGAATTGGGTCGTCAGCCCAACGATCAGG
AAATCGCTGATGCCCTTAGCCTCTCCATGCAAGAATGGCGTTCGGTTAAACTCGCCACCACCAATCGCGTTCCCCTTAGC
CTCAACGCTCGGGTCTCCTCTGGTCACTCTCAGTCTGACTCCGCTATGACCCTAGGTGACACCCTGATGGATGTTCATAG
CCAAATCCTGCAGGCCAACCAAGAAGATCGCATCGAACTTCAGCAAGCCTTAAACCAGCTCGAAGATCGGACCCGAATTA
TGATTGAGTCCGTCTTTTTCCAGCAGCTCTCTCGCCAAGAAGTGGCCAAGCGCATCGGTGTCAGCTCCGTCACTGTTACT
CGCAACATGAAGAAGGGCATCGACAAGCTAATCGATTTACTCCAACAGCAACAGCCTAACGCTCTGCAAACGGAACACTA
A

Upstream 100 bases:

>100_bases
ACCCAAAAAGTTTGATATTCCCGTGTTTTAACGATGGTTTTCCGAGTTCAGGATCTTCATAATGATTTTCAGATACAGGA
AAACAGGACTTTAAAAAACG

Downstream 100 bases:

>100_bases
ACACTTCCTCTCTAAACATAGGACATGCATCTTCCCTGTAGGCTTAAAGCCTACAGGGAATTTCTTTTGTTCCCCATCTG
CAAGGGTTCATCCTTCACGA

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MAYQTSLQSQTMELLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLTHQCAEPYEDLEQCGFLGLITAIERFDPSQGYAFS
SFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDAAKARQALTMELGRQPNDQEIADALSLSMQEWRSVKLATTNRVPLS
LNARVSSGHSQSDSAMTLGDTLMDVHSQILQANQEDRIELQQALNQLEDRTRIMIESVFFQQLSRQEVAKRIGVSSVTVT
RNMKKGIDKLIDLLQQQQPNALQTEH

Sequences:

>Translated_266_residues
MAYQTSLQSQTMELLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLTHQCAEPYEDLEQCGFLGLITAIERFDPSQGYAFS
SFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDAAKARQALTMELGRQPNDQEIADALSLSMQEWRSVKLATTNRVPLS
LNARVSSGHSQSDSAMTLGDTLMDVHSQILQANQEDRIELQQALNQLEDRTRIMIESVFFQQLSRQEVAKRIGVSSVTVT
RNMKKGIDKLIDLLQQQQPNALQTEH
>Mature_265_residues
AYQTSLQSQTMELLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLTHQCAEPYEDLEQCGFLGLITAIERFDPSQGYAFSS
FAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDAAKARQALTMELGRQPNDQEIADALSLSMQEWRSVKLATTNRVPLSL
NARVSSGHSQSDSAMTLGDTLMDVHSQILQANQEDRIELQQALNQLEDRTRIMIESVFFQQLSRQEVAKRIGVSSVTVTR
NMKKGIDKLIDLLQQQQPNALQTEH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=216, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1789448, Length=159, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789098, Length=250, Percent_Identity=27.2, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30429; Mature: 30298

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQTSLQSQTMELLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLTHQCAEPYEDLEQCG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
FLGLITAIERFDPSQGYAFSSFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDAAKARQ
HHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHH
ALTMELGRQPNDQEIADALSLSMQEWRSVKLATTNRVPLSLNARVSSGHSQSDSAMTLGD
HHHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCCHHHHHH
TLMDVHSQILQANQEDRIELQQALNQLEDRTRIMIESVFFQQLSRQEVAKRIGVSSVTVT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RNMKKGIDKLIDLLQQQQPNALQTEH
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AYQTSLQSQTMELLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLTHQCAEPYEDLEQCG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
FLGLITAIERFDPSQGYAFSSFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDAAKARQ
HHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHH
ALTMELGRQPNDQEIADALSLSMQEWRSVKLATTNRVPLSLNARVSSGHSQSDSAMTLGD
HHHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCCHHHHHH
TLMDVHSQILQANQEDRIELQQALNQLEDRTRIMIESVFFQQLSRQEVAKRIGVSSVTVT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RNMKKGIDKLIDLLQQQQPNALQTEH
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]