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The map label for this gene is csgA [H]

Identifier: 158338067

GI number: 158338067

Start: 4991857

End: 4992609

Strand: Reverse

Name: csgA [H]

Synonym: AM1_4954

Alternate gene names: 158338067

Gene position: 4992609-4991857 (Counterclockwise)

Preceding gene: 158338068

Following gene: 158338065

Centisome position: 76.77

GC content: 49.54

Gene sequence:

>753_bases
ATGAGTGAGTCCAATGCCCTGGTGATCGGGGCCAGTCAAGGCATTGGCTTGGGGTTTGTGCAGCATCTGCTTCAGAGGCC
CCATATTCAAACTATTTACGCCACCTATCGTTCCCAAGACTCAGCTGTAGGATTACTTACTCTAGCGGCCCAATATCCAG
AACGGGTGTCCTGCATTGCCGTAGATATTACTCAAGAGACGCAAATTGCAGATTGCTGTAATCAGATTCAATCTGAGGGT
GTTCTGCTCTACTGGGTGATTAACTGCGTGGGCATTCTCCACGAAGGCGATCTACAGCCGGAGAAAGGATTGCGGCAGCT
CAATGCTGAACAGTTAACGCGATATTTCCAAGTCAATAGTATTGGGGCAGCTCTATGGGCTAAACATCTATTGCCGTTGT
TGAAGCATGATCATCCCAGTATCTTTGCGACTATCTCGGCCAAGATCGGCAGCATTGGCGATAACCATTTAGGGGGATGG
TATGGGTATCGAGCCTCCAAAGCAGCTTTGAATATGCTGATGCGAACTACTTCGATTGAGTATCGCCGTCGCAGTCCTCA
AACCATTGTGGTGACGCTACACCCAGGCACGACGGATACCAAACTCTCTAAACCCTTTCAGCGGAATGTCCCACCGGAAA
AACTATTCTCGGTTGATCGAACCGTCACTCAACTGTTGACGGTTCTGGACGGGCTGGGAAAAGAAGACAGTGGCCAGTTC
TTTTCCTGGGATGGTAGTCTGTTGCCTTGGTAG

Upstream 100 bases:

>100_bases
CGTTAAGCTGGCAAAAGCCCCATCTCTATCGCTGTGATTTTGCCAATCAAACCTGCCCCGATGTTAGTAGCCAGCTTCCT
ATCGTTAGTCCACCGTTGGT

Downstream 100 bases:

>100_bases
GGTATGCTGGCCTGCATCAATTGGGTGAGGCATTATTTCTTTTCCTCAGATAATGCTGCCTGAAATTCGGTGTAGTTGCC
CCCTAACGCTTCCACAATCG

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: C signal [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSESNALVIGASQGIGLGFVQHLLQRPHIQTIYATYRSQDSAVGLLTLAAQYPERVSCIAVDITQETQIADCCNQIQSEG
VLLYWVINCVGILHEGDLQPEKGLRQLNAEQLTRYFQVNSIGAALWAKHLLPLLKHDHPSIFATISAKIGSIGDNHLGGW
YGYRASKAALNMLMRTTSIEYRRRSPQTIVVTLHPGTTDTKLSKPFQRNVPPEKLFSVDRTVTQLLTVLDGLGKEDSGQF
FSWDGSLLPW

Sequences:

>Translated_250_residues
MSESNALVIGASQGIGLGFVQHLLQRPHIQTIYATYRSQDSAVGLLTLAAQYPERVSCIAVDITQETQIADCCNQIQSEG
VLLYWVINCVGILHEGDLQPEKGLRQLNAEQLTRYFQVNSIGAALWAKHLLPLLKHDHPSIFATISAKIGSIGDNHLGGW
YGYRASKAALNMLMRTTSIEYRRRSPQTIVVTLHPGTTDTKLSKPFQRNVPPEKLFSVDRTVTQLLTVLDGLGKEDSGQF
FSWDGSLLPW
>Mature_249_residues
SESNALVIGASQGIGLGFVQHLLQRPHIQTIYATYRSQDSAVGLLTLAAQYPERVSCIAVDITQETQIADCCNQIQSEGV
LLYWVINCVGILHEGDLQPEKGLRQLNAEQLTRYFQVNSIGAALWAKHLLPLLKHDHPSIFATISAKIGSIGDNHLGGWY
GYRASKAALNMLMRTTSIEYRRRSPQTIVVTLHPGTTDTKLSKPFQRNVPPEKLFSVDRTVTQLLTVLDGLGKEDSGQFF
SWDGSLLPW

Specific function: Cell-cell signaling protein required for fruiting body morphogenesis. Necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hour of starvation [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To yeast YKL071W [H]

Homologues:

Organism=Caenorhabditis elegans, GI17558994, Length=260, Percent_Identity=28.4615384615385, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558996, Length=269, Percent_Identity=24.5353159851301, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558990, Length=271, Percent_Identity=25.0922509225092, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17542178, Length=217, Percent_Identity=28.110599078341, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558992, Length=262, Percent_Identity=30.5343511450382, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559964, Length=267, Percent_Identity=26.2172284644195, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17541382, Length=270, Percent_Identity=24.0740740740741, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193207563, Length=262, Percent_Identity=25.1908396946565, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322227, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640547, Length=262, Percent_Identity=27.8625954198473, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 27702; Mature: 27571

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNALVIGASQGIGLGFVQHLLQRPHIQTIYATYRSQDSAVGLLTLAAQYPERVSCIA
CCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
VDITQETQIADCCNQIQSEGVLLYWVINCVGILHEGDLQPEKGLRQLNAEQLTRYFQVNS
EEECCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHH
IGAALWAKHLLPLLKHDHPSIFATISAKIGSIGDNHLGGWYGYRASKAALNMLMRTTSIE
HHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YRRRSPQTIVVTLHPGTTDTKLSKPFQRNVPPEKLFSVDRTVTQLLTVLDGLGKEDSGQF
HHCCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSWDGSLLPW
EEECCCCCCC
>Mature Secondary Structure 
SESNALVIGASQGIGLGFVQHLLQRPHIQTIYATYRSQDSAVGLLTLAAQYPERVSCIA
CCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
VDITQETQIADCCNQIQSEGVLLYWVINCVGILHEGDLQPEKGLRQLNAEQLTRYFQVNS
EEECCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHH
IGAALWAKHLLPLLKHDHPSIFATISAKIGSIGDNHLGGWYGYRASKAALNMLMRTTSIE
HHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YRRRSPQTIVVTLHPGTTDTKLSKPFQRNVPPEKLFSVDRTVTQLLTVLDGLGKEDSGQF
HHCCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSWDGSLLPW
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2152896; 2107980; 2118510; 1372277 [H]