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The map label for this gene is ispH

Identifier: 158338063

GI number: 158338063

Start: 4987642

End: 4988889

Strand: Reverse

Name: ispH

Synonym: AM1_4950

Alternate gene names: 158338063

Gene position: 4988889-4987642 (Counterclockwise)

Preceding gene: 158338064

Following gene: 158338059

Centisome position: 76.71

GC content: 47.28

Gene sequence:

>1248_bases
ATGGATACAAAAGCCTTTAAGCGATCGCTGCATAAATCAGAGCAGTATCATCGGAAGGGATTTGGTCACGAAGAAGAAGT
GACCAACTTGCTGCAGTCTGAGTACAATAGCAAGCTCATCCAACAAATTCGCAATAATGGGTATCGCCTTCAACAGGGAG
ATGTCACCATTCACTTAGCAGAAGCCTTTGGCTTTTGCTGGGGGGTTGAGCGGGCCATTGCCTTGGCCTATGAAACGCGC
CGTCAGTTTCCCCAAGAGCGCATTTGGATAACCAACGAAATTATTCATAATCCCTCAGTCAACAAGAACCTGCGGGATAT
GAAAGTAGAGTTTATTCCGGTGCAACCTGATGGCCAGAAAGACTTCACCGTGGTGGGCCAAAATGATGTCGTCATCCTGC
CAGCCTTTGGTGCCAGTGTTCAGGAAATGCAGTTACTTCACGATAAAGGCTGCACCATAATGGATACCACCTGTCCCTGG
GTGTCCAAGGTTTGGACCTCCGTCGAAAAACATAAGAAAGGGTCCTATACCTCGATTATTCATGGCAAGTACAAGCATGA
GGAGACCATTGCCACAAGCTCCTTTGCAGGGACCTATCTCGTCTTACTCAACCTGGAAGAAGCAGAATATGTCGCAGACT
ATATTTTGAATCCAGGAGACACCCCCTCAGAACGGGAGCAGCGTCGGGCCGATTTTATGGCCAAGTTTGCCAATGCTCAC
TCCCCTGGTTTTGACCCAGAAACAGATTTAGAACGGGTCGGGATTGCTAATCAGACGACGATGTTGAAAGGTGAAACCGA
GCAAATAGGTAAGCTGTTTGAACGCACCGTTATGCGTAAGTATGGCCCTGCCCAGGTCAACGAGCATTTCCTCAGTTTCA
ACACCATTTGCGATGCCACCCAAGAGCGTCAGGATGCGATTTTTCAATTGGTCGATGAACCCTTGGATTTAATGGTCGTG
ATTGGCGGCTTTAATTCATCCAATACGACCCATCTCCAAGAGATCTCAATTGATAAAAATATTCCTTCCTATCACATTGA
TAGTGTCGATCGCATTGGTCCAGGCAATCGAGTTGAACATAAACCTCTTAACGCCGATCTAACAGTTACAGACAATTGGT
TGCCGAATGGTCCCCTTGCCATTGGGGTGACGTCAGGGGCATCAACACCAGACAAAGTGGTGTCTGATATCGTGGAAAAA
ATCTTCTCGATTAAGAGTGCTTCTGCCGAAGTGGTGGGGGTTAGATAA

Upstream 100 bases:

>100_bases
CCTTGTTGCAGCAAGATTGAATTGGCCTCTTTCAGTGTCTTAAAAGTTCTTTTAGGGACTCATCTGAAGGGCGTTTCCCC
CTAGATGCAGGAATATATTC

Downstream 100 bases:

>100_bases
TCCTTCGATAACGCCCAGACTAACGAGTCACCACCATAATAATCGTCATCACAAAAAAGCCACTCGCACCCACAATCGTG
ATGATGTCGGTGACTCGTTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAEAFGFCWGVERAIALAYETR
RQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQKDFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPW
VSKVWTSVEKHKKGSYTSIIHGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAH
SPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDATQERQDAIFQLVDEPLDLMVV
IGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHKPLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEK
IFSIKSASAEVVGVR

Sequences:

>Translated_415_residues
MDTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAEAFGFCWGVERAIALAYETR
RQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQKDFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPW
VSKVWTSVEKHKKGSYTSIIHGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAH
SPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDATQERQDAIFQLVDEPLDLMVV
IGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHKPLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEK
IFSIKSASAEVVGVR
>Mature_415_residues
MDTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAEAFGFCWGVERAIALAYETR
RQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQKDFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPW
VSKVWTSVEKHKKGSYTSIIHGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAH
SPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDATQERQDAIFQLVDEPLDLMVV
IGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHKPLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEK
IFSIKSASAEVVGVR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:
GO:0008299: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0016491: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0019288: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0046872: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0051536: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0051538: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0051745: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
GO:0055114: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_ACAM1 (B0C4N8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519239.1
- GeneID:   5683750
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4950
- HOGENOM:   HBG286221
- OMA:   REENIEF
- ProtClustDB:   PRK13371
- BioCyc:   AMAR329726:AM1_4950-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 46697; Mature: 46697

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEH
EAFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQK
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHCCCCCCCCCCCCEEEEEECCCCCCC
DFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPWVSKVWTSVEKHKKGSYTSII
CEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
HGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAH
CCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
SPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDAT
CCCCCCCCCHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QERQDAIFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEH
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
KPLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEKIFSIKSASAEVVGVR
CCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MDTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEH
EAFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQK
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHCCCCCCCCCCCCEEEEEECCCCCCC
DFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPWVSKVWTSVEKHKKGSYTSII
CEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
HGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRRADFMAKFANAH
CCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
SPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEHFLSFNTICDAT
CCCCCCCCCHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QERQDAIFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEH
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
KPLNADLTVTDNWLPNGPLAIGVTSGASTPDKVVSDIVEKIFSIKSASAEVVGVR
CCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA