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The map label for this gene is nadE [H]

Identifier: 158338012

GI number: 158338012

Start: 4946686

End: 4948395

Strand: Direct

Name: nadE [H]

Synonym: AM1_4899

Alternate gene names: 158338012

Gene position: 4946686-4948395 (Clockwise)

Preceding gene: 158338011

Following gene: 158338014

Centisome position: 76.06

GC content: 52.34

Gene sequence:

>1710_bases
ATGAAGATTGCGATCGCACAATTGAATCCCACGATTGGTGATTTGGTCGGGAATGCTGAAGCTATTGCAGCGGCGGCCAC
GGCAGCCGAACAGGAGGGGGCTACCTTACTCTTGACTCCAGAGCTATCCCTATGTGGCTATCCCCCTAGAGACTTGTTGC
TCGACCCCAGCTTTGTCGAGGCGGCCCAGACTACCTTGGACCAGCTCGCCACCCATTTACCGGCCAAACTCCACGTGCTG
GTAGGCAGCGTGCAGCAAAACGCAGCTCAGGGGGGAAAACCCTTATTCAATAGCATTGTTCATTTGCATAACGGCGAGAT
TCAGCAGTGCTTTCATAAGCATTTGCTGCCCACCTACGATGTGTTCGATGAAGATCGCTATTTTGAACCCGGCCACCAGC
CCAATGTGCTGGACCTGAAAGAAGGCAAAATTGGGGTCACTATCTGTGAAGATCTGTGGAACAACTCAGAGTTCTGGGAG
AAACAAGCCTACGCCGTGGATCCAGTTGCTGAACTGATGAGCTACAACGTGGATTTGATCATCAACTTATCTGCCTCTCC
CTTCTGCGTCGGCAAACAAGCACTGCGAGAAGCGATGTTACGGCACCATGCTCAGCAATACCACTGTCCCCTGATCTATG
CCAATCAGGTGGGTGGAAATGACGACTTGATTTTTGATGGCCGCAGTGTCGCCGTCAACCGCCAGGGAGATGTAGTGGGA
CGGGCCAAGGGCTTTGCCCCTGACTTGTTGCTCTTTGATTACGAGGCCCATGATCTGCTGCCCAGTCGAATCGAGCCTGC
GGTATCGGATATCAATGCTGAAATCTGGTCAGCCTTGGTATTGGGATTGCGAGACTATGCCCAAAAATGTGGATTTAGTC
AAGCCGTTCTGGGATTGAGTGGCGGTATTGACTCTTCCCTAGTGGCCGCCATTGCCGCTGCCGCCTTGGGTCCTGAGAAT
GTATTGGGTGTGCTGATGCCTTCACCCTACAGTTCTGATCATTCCATCAAGGATGCGGAAGATTTGGCCCAGAACTTGGG
AATGCCAACAACACAGTTAGCGATCGCATCAGCAATGCAGACCTACGACCAGATCTTAAGCTCCGTTTTTGCCGGGACCG
AACCCGGTGTCGCCGAAGAAAATATTCAAGCCCGGATTCGAGGTAATTTGCTGATGGCCATTGCTAACAAATTTGGCCGT
TTACTCATTTCCACGGGCAATAAATCGGAAATGGCCGTGGGCTACTGCACCCTCTACGGGGATATGAATGGAGGTTTAGC
AGCCATTGCAGATGTGCCTAAAACTCGGGTGTATGAGCTATGCCGTTGGCTGAATACCCACACGCCACCCGAACTGGAAG
AGCATGGCATCATCATTCCTGACTCTGTGATCACCAAGCCCCCTAGCGCTGAACTTAAGCCCGGCCAGGTGGATCAAGAC
TCTTTGCCTGATTATGATGTTCTCGATGATATTCTCTATCGCTATGTCGATCACCACCAATCGAGCCAACAAATAATCGC
GGCAGGTCATCCTACCGCGATCGTGGACCGAATTATTCAAATGGTCAAAAGGGCTGAGTTCAAACGCCGCCAAGCCCCTC
CTGGGCTCAAGATTACGGACCGAGCGTTTGGCACAGGTTGGCGTATGCCCATTGCGGCTCGAACCACCTGGCCACCTACA
GCAGCTCAGCCGGTATCCGTTGAGACCTAA

Upstream 100 bases:

>100_bases
CTGCCGGGGAGCAGGTCGTCCGGCCACGTTATATCGGTTTGATGTGGAAGCCTTTGCCCCTTTTCGAGATAAACCGCTAG
TGTTTGTGTAGGAACGAAAC

Downstream 100 bases:

>100_bases
TGGCTCGCCAGCGGAACGATTAACCGATTCCAGCCACCACCAATGCGGCTTTTGCCGTTCCACCAAAAGGGAGGAGGTGG
TTAATCACTGTATTCAGTAT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MKIAIAQLNPTIGDLVGNAEAIAAAATAAEQEGATLLLTPELSLCGYPPRDLLLDPSFVEAAQTTLDQLATHLPAKLHVL
VGSVQQNAAQGGKPLFNSIVHLHNGEIQQCFHKHLLPTYDVFDEDRYFEPGHQPNVLDLKEGKIGVTICEDLWNNSEFWE
KQAYAVDPVAELMSYNVDLIINLSASPFCVGKQALREAMLRHHAQQYHCPLIYANQVGGNDDLIFDGRSVAVNRQGDVVG
RAKGFAPDLLLFDYEAHDLLPSRIEPAVSDINAEIWSALVLGLRDYAQKCGFSQAVLGLSGGIDSSLVAAIAAAALGPEN
VLGVLMPSPYSSDHSIKDAEDLAQNLGMPTTQLAIASAMQTYDQILSSVFAGTEPGVAEENIQARIRGNLLMAIANKFGR
LLISTGNKSEMAVGYCTLYGDMNGGLAAIADVPKTRVYELCRWLNTHTPPELEEHGIIIPDSVITKPPSAELKPGQVDQD
SLPDYDVLDDILYRYVDHHQSSQQIIAAGHPTAIVDRIIQMVKRAEFKRRQAPPGLKITDRAFGTGWRMPIAARTTWPPT
AAQPVSVET

Sequences:

>Translated_569_residues
MKIAIAQLNPTIGDLVGNAEAIAAAATAAEQEGATLLLTPELSLCGYPPRDLLLDPSFVEAAQTTLDQLATHLPAKLHVL
VGSVQQNAAQGGKPLFNSIVHLHNGEIQQCFHKHLLPTYDVFDEDRYFEPGHQPNVLDLKEGKIGVTICEDLWNNSEFWE
KQAYAVDPVAELMSYNVDLIINLSASPFCVGKQALREAMLRHHAQQYHCPLIYANQVGGNDDLIFDGRSVAVNRQGDVVG
RAKGFAPDLLLFDYEAHDLLPSRIEPAVSDINAEIWSALVLGLRDYAQKCGFSQAVLGLSGGIDSSLVAAIAAAALGPEN
VLGVLMPSPYSSDHSIKDAEDLAQNLGMPTTQLAIASAMQTYDQILSSVFAGTEPGVAEENIQARIRGNLLMAIANKFGR
LLISTGNKSEMAVGYCTLYGDMNGGLAAIADVPKTRVYELCRWLNTHTPPELEEHGIIIPDSVITKPPSAELKPGQVDQD
SLPDYDVLDDILYRYVDHHQSSQQIIAAGHPTAIVDRIIQMVKRAEFKRRQAPPGLKITDRAFGTGWRMPIAARTTWPPT
AAQPVSVET
>Mature_569_residues
MKIAIAQLNPTIGDLVGNAEAIAAAATAAEQEGATLLLTPELSLCGYPPRDLLLDPSFVEAAQTTLDQLATHLPAKLHVL
VGSVQQNAAQGGKPLFNSIVHLHNGEIQQCFHKHLLPTYDVFDEDRYFEPGHQPNVLDLKEGKIGVTICEDLWNNSEFWE
KQAYAVDPVAELMSYNVDLIINLSASPFCVGKQALREAMLRHHAQQYHCPLIYANQVGGNDDLIFDGRSVAVNRQGDVVG
RAKGFAPDLLLFDYEAHDLLPSRIEPAVSDINAEIWSALVLGLRDYAQKCGFSQAVLGLSGGIDSSLVAAIAAAALGPEN
VLGVLMPSPYSSDHSIKDAEDLAQNLGMPTTQLAIASAMQTYDQILSSVFAGTEPGVAEENIQARIRGNLLMAIANKFGR
LLISTGNKSEMAVGYCTLYGDMNGGLAAIADVPKTRVYELCRWLNTHTPPELEEHGIIIPDSVITKPPSAELKPGQVDQD
SLPDYDVLDDILYRYVDHHQSSQQIIAAGHPTAIVDRIIQMVKRAEFKRRQAPPGLKITDRAFGTGWRMPIAARTTWPPT
AAQPVSVET

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:
GO:0000166: NAD+ synthetase
GO:0003952: NAD+ synthetase
GO:0005524: NAD+ synthetase
GO:0006807: NAD+ synthetase
GO:0009435: NAD+ synthetase
GO:0016810: NAD+ synthetase
GO:0016874: NAD+ synthetase

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61744; Mature: 61744

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAQLNPTIGDLVGNAEAIAAAATAAEQEGATLLLTPELSLCGYPPRDLLLDPSFVE
CEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHEECCHHHH
AAQTTLDQLATHLPAKLHVLVGSVQQNAAQGGKPLFNSIVHLHNGEIQQCFHKHLLPTYD
HHHHHHHHHHHHCCHHEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGHQPNVLDLKEGKIGVTICEDLWNNSEFWEKQAYAVDPVAELMSYNVDLI
CCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCCHHHHHHCCCCCHHHHHHHCCCEEE
INLSASPFCVGKQALREAMLRHHAQQYHCPLIYANQVGGNDDLIFDGRSVAVNRQGDVVG
EECCCCCCHHCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEEECCCCEEE
RAKGFAPDLLLFDYEAHDLLPSRIEPAVSDINAEIWSALVLGLRDYAQKCGFSQAVLGLS
CCCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
GGIDSSLVAAIAAAALGPENVLGVLMPSPYSSDHSIKDAEDLAQNLGMPTTQLAIASAMQ
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
TYDQILSSVFAGTEPGVAEENIQARIRGNLLMAIANKFGRLLISTGNKSEMAVGYCTLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCEEEEECCCCCCEEEEEEEEEE
DMNGGLAAIADVPKTRVYELCRWLNTHTPPELEEHGIIIPDSVITKPPSAELKPGQVDQD
CCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCEECCCHHHCCCCCCCCCCCCCCCC
SLPDYDVLDDILYRYVDHHQSSQQIIAAGHPTAIVDRIIQMVKRAEFKRRQAPPGLKITD
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
RAFGTGWRMPIAARTTWPPTAAQPVSVET
CCCCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAIAQLNPTIGDLVGNAEAIAAAATAAEQEGATLLLTPELSLCGYPPRDLLLDPSFVE
CEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHEECCHHHH
AAQTTLDQLATHLPAKLHVLVGSVQQNAAQGGKPLFNSIVHLHNGEIQQCFHKHLLPTYD
HHHHHHHHHHHHCCHHEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGHQPNVLDLKEGKIGVTICEDLWNNSEFWEKQAYAVDPVAELMSYNVDLI
CCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCCHHHHHHCCCCCHHHHHHHCCCEEE
INLSASPFCVGKQALREAMLRHHAQQYHCPLIYANQVGGNDDLIFDGRSVAVNRQGDVVG
EECCCCCCHHCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEEECCCCEEE
RAKGFAPDLLLFDYEAHDLLPSRIEPAVSDINAEIWSALVLGLRDYAQKCGFSQAVLGLS
CCCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
GGIDSSLVAAIAAAALGPENVLGVLMPSPYSSDHSIKDAEDLAQNLGMPTTQLAIASAMQ
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
TYDQILSSVFAGTEPGVAEENIQARIRGNLLMAIANKFGRLLISTGNKSEMAVGYCTLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCEEEEECCCCCCEEEEEEEEEE
DMNGGLAAIADVPKTRVYELCRWLNTHTPPELEEHGIIIPDSVITKPPSAELKPGQVDQD
CCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCEECCCHHHCCCCCCCCCCCCCCCC
SLPDYDVLDDILYRYVDHHQSSQQIIAAGHPTAIVDRIIQMVKRAEFKRRQAPPGLKITD
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
RAFGTGWRMPIAARTTWPPTAAQPVSVET
CCCCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]