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The map label for this gene is pep [H]

Identifier: 158337998

GI number: 158337998

Start: 4933972

End: 4935816

Strand: Direct

Name: pep [H]

Synonym: AM1_4885

Alternate gene names: 158337998

Gene position: 4933972-4935816 (Clockwise)

Preceding gene: 158337997

Following gene: 158337999

Centisome position: 75.86

GC content: 46.61

Gene sequence:

>1845_bases
TTGTGGAATTATGAAAAGCTGAGTGTTCCCTTGAAAAAAGGCGATCGCTATTTTTTCTCCAAAAATAATGGTCTCCAAAA
TCAAAGCGTGCTCTATTCTCGAAAGAGTTTGACTTCAGAGCCCGAAATGATTCTCGACCCGAATCTCCTTTCCGAAGATG
GCACGGTCGCCCTATCGGGATTAGCCCTGAGCGATAATGCTCAATATTTAGCCTATGGATTATCAGAATCCGGTTCCGAT
TGGCAAACCTGGCACATTCGAGATCTATCCACAGGCGAAGATTTATCGGAGCAACTGCAATGGATTAAGTTCTCAGGGGC
AGCGTGGACCGCAGATCATCAGGGGTTCTTCTATGGGCGATACGATGAACCGGATGAAAATAATAAACTTGAAGGCGTTA
ACTATTATCAAAAGCTTTTTTACCATCGTTTAGGAACGCCCCAGTCGGCAGATCTGCTGATTTATGAACGGCCTGACCAA
AAAGAATGGGGATTTCAAGCCGTCGTATCAGAAGATGGAGATTACCTCCTGATCCATGTGTGGCTGGGGACAGATGCCCG
TAATTTATTGTTTTACAAAGATCTTAAATCGCCTGAATCTCCGGTTACTGAGTTAATTTCCATCTTTGAAGCGAGCTACA
GTTTTATCGGCAATCAAAAGTCCCTGTTCTGGGTCAAAACGGATTTAAATGCACCTCGGGGGCGGGTGATTGCCATCGAT
CTTCATCACCCTGACCAAGAACACTGGCAAACGATCATCTCTGAATCCGCCGATACCCTGGAAGGGATCGGTATTCTCAA
CCATCAATTTGTCGCAACCTACCTCAAAGATGCGAGATCGCAAGTCAAATGCTTTGCCTTAGATGGGCAGGAGCTAGGAG
AAGTATCGCTTCCTGGAGTCGGTAGTGTCAGCGGGTTCTATGGCAAGACAACCGAAACGAAAACCTTCTACAGTTTCACG
AGCTTTACGACACCGACCTCGATCTATCGTTTTGATCTAGAATCTGGTGAATCAACCCTCTACTGGCAACCTCAGGTTGA
CTTTAATCCCACGGAGTATGAAACTCAGCAGGTCTTCTTTACCAGTAAAGATGGTACTCAAATTCCCTTATTTCTCAGCC
ATAAGAAAGGCTTGATCAAAGATGGGAAAACTCCTGTCTATCTCTATGGCTATGGGGGGTTTAATGTATTCCTGACTCCG
TCTTTTTCCCCGGTTCAACTCGCCTGGATGGAAATGGGCGGTATCTTTGCCCTACCCACATTACGGGGTGGAGGCGAATA
CGGTGAAGAGTGGCATCAGGCTGGGATGAAGCATCATAAGCAAACGGTTTTTGATGATTTTATTGCAGCTGCCGAGTATC
TGATTACAGCAGGATATACCTGTACAGACAAATTAGCGATCGCAGGGGGAAGCAATGGAGGATTGTTGGTTGGAGCCTGT
ATGACACAACGGCCAGATTTATTTGGTGCCGCTTTGCCAGCGGTTGGCGTCATGGATATGTTGCGATTTCATCAGTTTAC
GATTGGCTGGGCTTGGTGTGCAGAATATGGTAGCTCCGAAAATGCCGAGGATTTCCCTGTCCTATATGCCTATTCCCCCT
TACATAATCTCCAAGGGGACACCTCTTACCCAGCTACTTTGATTACTACCGCAGACCACGATGATCGCGTTGTACCAGCT
CATAGTTTCAAGTTTGCGGCTGCGCTCCAAGCCGCGCATTCAGGGATGGCTCCAACCTTAATTCGAATTGAAACGAAAGC
AGGGCATGGTGCTGGTAAGCCAACTCAGAAACAGATAGAAGAAGCCAGCGATCGGCTTGCTTTTGTCAAGCATGTTTTAG
CTTGA

Upstream 100 bases:

>100_bases
TGCTGACACTCAAGCTTGGATTCAGGCCCAAAATGCTTTGACGTTTGAGTTTTTGGAATCCATACCAGAGCGATCGCAAA
TCTAATCCCGTCTCACTCAG

Downstream 100 bases:

>100_bases
ATTAGGGGATTGGTATTAGGCTTGATTGGTGCCACATCTAGTAAAATCGCATTGTCCGGCATTAGTTTCTGAACAGGATC
TACAAGGTCGAACTGGGTTT

Product: prolyl endopeptidase PEP

Products: NA

Alternate protein names: Post-proline cleaving enzyme; Proline-specific endopeptidase; PE; PSE [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MWNYEKLSVPLKKGDRYFFSKNNGLQNQSVLYSRKSLTSEPEMILDPNLLSEDGTVALSGLALSDNAQYLAYGLSESGSD
WQTWHIRDLSTGEDLSEQLQWIKFSGAAWTADHQGFFYGRYDEPDENNKLEGVNYYQKLFYHRLGTPQSADLLIYERPDQ
KEWGFQAVVSEDGDYLLIHVWLGTDARNLLFYKDLKSPESPVTELISIFEASYSFIGNQKSLFWVKTDLNAPRGRVIAID
LHHPDQEHWQTIISESADTLEGIGILNHQFVATYLKDARSQVKCFALDGQELGEVSLPGVGSVSGFYGKTTETKTFYSFT
SFTTPTSIYRFDLESGESTLYWQPQVDFNPTEYETQQVFFTSKDGTQIPLFLSHKKGLIKDGKTPVYLYGYGGFNVFLTP
SFSPVQLAWMEMGGIFALPTLRGGGEYGEEWHQAGMKHHKQTVFDDFIAAAEYLITAGYTCTDKLAIAGGSNGGLLVGAC
MTQRPDLFGAALPAVGVMDMLRFHQFTIGWAWCAEYGSSENAEDFPVLYAYSPLHNLQGDTSYPATLITTADHDDRVVPA
HSFKFAAALQAAHSGMAPTLIRIETKAGHGAGKPTQKQIEEASDRLAFVKHVLA

Sequences:

>Translated_614_residues
MWNYEKLSVPLKKGDRYFFSKNNGLQNQSVLYSRKSLTSEPEMILDPNLLSEDGTVALSGLALSDNAQYLAYGLSESGSD
WQTWHIRDLSTGEDLSEQLQWIKFSGAAWTADHQGFFYGRYDEPDENNKLEGVNYYQKLFYHRLGTPQSADLLIYERPDQ
KEWGFQAVVSEDGDYLLIHVWLGTDARNLLFYKDLKSPESPVTELISIFEASYSFIGNQKSLFWVKTDLNAPRGRVIAID
LHHPDQEHWQTIISESADTLEGIGILNHQFVATYLKDARSQVKCFALDGQELGEVSLPGVGSVSGFYGKTTETKTFYSFT
SFTTPTSIYRFDLESGESTLYWQPQVDFNPTEYETQQVFFTSKDGTQIPLFLSHKKGLIKDGKTPVYLYGYGGFNVFLTP
SFSPVQLAWMEMGGIFALPTLRGGGEYGEEWHQAGMKHHKQTVFDDFIAAAEYLITAGYTCTDKLAIAGGSNGGLLVGAC
MTQRPDLFGAALPAVGVMDMLRFHQFTIGWAWCAEYGSSENAEDFPVLYAYSPLHNLQGDTSYPATLITTADHDDRVVPA
HSFKFAAALQAAHSGMAPTLIRIETKAGHGAGKPTQKQIEEASDRLAFVKHVLA
>Mature_614_residues
MWNYEKLSVPLKKGDRYFFSKNNGLQNQSVLYSRKSLTSEPEMILDPNLLSEDGTVALSGLALSDNAQYLAYGLSESGSD
WQTWHIRDLSTGEDLSEQLQWIKFSGAAWTADHQGFFYGRYDEPDENNKLEGVNYYQKLFYHRLGTPQSADLLIYERPDQ
KEWGFQAVVSEDGDYLLIHVWLGTDARNLLFYKDLKSPESPVTELISIFEASYSFIGNQKSLFWVKTDLNAPRGRVIAID
LHHPDQEHWQTIISESADTLEGIGILNHQFVATYLKDARSQVKCFALDGQELGEVSLPGVGSVSGFYGKTTETKTFYSFT
SFTTPTSIYRFDLESGESTLYWQPQVDFNPTEYETQQVFFTSKDGTQIPLFLSHKKGLIKDGKTPVYLYGYGGFNVFLTP
SFSPVQLAWMEMGGIFALPTLRGGGEYGEEWHQAGMKHHKQTVFDDFIAAAEYLITAGYTCTDKLAIAGGSNGGLLVGAC
MTQRPDLFGAALPAVGVMDMLRFHQFTIGWAWCAEYGSSENAEDFPVLYAYSPLHNLQGDTSYPATLITTADHDDRVVPA
HSFKFAAALQAAHSGMAPTLIRIETKAGHGAGKPTQKQIEEASDRLAFVKHVLA

Specific function: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond [H]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=637, Percent_Identity=46.3108320251177, Blast_Score=556, Evalue=1e-158,
Organism=Homo sapiens, GI108860686, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI284172420, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI284172413, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI70778815, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI284172438, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI284172431, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788150, Length=621, Percent_Identity=24.6376811594203, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24583414, Length=643, Percent_Identity=47.2783825816485, Blast_Score=574, Evalue=1e-164,
Organism=Drosophila melanogaster, GI221510989, Length=640, Percent_Identity=44.375, Blast_Score=522, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002471
- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: =3.4.21.26 [H]

Molecular weight: Translated: 68531; Mature: 68531

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00708 PRO_ENDOPEP_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNYEKLSVPLKKGDRYFFSKNNGLQNQSVLYSRKSLTSEPEMILDPNLLSEDGTVALSG
CCCCCEEECCCCCCCEEEEECCCCCCCCHHEEEHHHCCCCCCEEECCCCCCCCCCEEEEE
LALSDNAQYLAYGLSESGSDWQTWHIRDLSTGEDLSEQLQWIKFSGAAWTADHQGFFYGR
EEECCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHEEEECCCEEECCCCCEEEEC
YDEPDENNKLEGVNYYQKLFYHRLGTPQSADLLIYERPDQKEWGFQAVVSEDGDYLLIHV
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEE
WLGTDARNLLFYKDLKSPESPVTELISIFEASYSFIGNQKSLFWVKTDLNAPRGRVIAID
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEE
LHHPDQEHWQTIISESADTLEGIGILNHQFVATYLKDARSQVKCFALDGQELGEVSLPGV
ECCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC
GSVSGFYGKTTETKTFYSFTSFTTPTSIYRFDLESGESTLYWQPQVDFNPTEYETQQVFF
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEE
TSKDGTQIPLFLSHKKGLIKDGKTPVYLYGYGGFNVFLTPSFSPVQLAWMEMGGIFALPT
ECCCCCEEEEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEECCCEEEECE
LRGGGEYGEEWHQAGMKHHKQTVFDDFIAAAEYLITAGYTCTDKLAIAGGSNGGLLVGAC
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEE
MTQRPDLFGAALPAVGVMDMLRFHQFTIGWAWCAEYGSSENAEDFPVLYAYSPLHNLQGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHCCCCC
TSYPATLITTADHDDRVVPAHSFKFAAALQAAHSGMAPTLIRIETKAGHGAGKPTQKQIE
CCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHH
EASDRLAFVKHVLA
HHHHHHHHHHHHHC
>Mature Secondary Structure
MWNYEKLSVPLKKGDRYFFSKNNGLQNQSVLYSRKSLTSEPEMILDPNLLSEDGTVALSG
CCCCCEEECCCCCCCEEEEECCCCCCCCHHEEEHHHCCCCCCEEECCCCCCCCCCEEEEE
LALSDNAQYLAYGLSESGSDWQTWHIRDLSTGEDLSEQLQWIKFSGAAWTADHQGFFYGR
EEECCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHEEEECCCEEECCCCCEEEEC
YDEPDENNKLEGVNYYQKLFYHRLGTPQSADLLIYERPDQKEWGFQAVVSEDGDYLLIHV
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEE
WLGTDARNLLFYKDLKSPESPVTELISIFEASYSFIGNQKSLFWVKTDLNAPRGRVIAID
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEE
LHHPDQEHWQTIISESADTLEGIGILNHQFVATYLKDARSQVKCFALDGQELGEVSLPGV
ECCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC
GSVSGFYGKTTETKTFYSFTSFTTPTSIYRFDLESGESTLYWQPQVDFNPTEYETQQVFF
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEE
TSKDGTQIPLFLSHKKGLIKDGKTPVYLYGYGGFNVFLTPSFSPVQLAWMEMGGIFALPT
ECCCCCEEEEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEECCCEEEECE
LRGGGEYGEEWHQAGMKHHKQTVFDDFIAAAEYLITAGYTCTDKLAIAGGSNGGLLVGAC
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEE
MTQRPDLFGAALPAVGVMDMLRFHQFTIGWAWCAEYGSSENAEDFPVLYAYSPLHNLQGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHCCCCC
TSYPATLITTADHDDRVVPAHSFKFAAALQAAHSGMAPTLIRIETKAGHGAGKPTQKQIE
CCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHH
EASDRLAFVKHVLA
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840588; 7764331 [H]