Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 158337985

GI number: 158337985

Start: 4923851

End: 4925983

Strand: Direct

Name: topA [H]

Synonym: AM1_4872

Alternate gene names: 158337985

Gene position: 4923851-4925983 (Clockwise)

Preceding gene: 158337984

Following gene: 158337987

Centisome position: 75.71

GC content: 51.01

Gene sequence:

>2133_bases
ATGTCTCGCCTGCTCATTGTCGAATCCCCAGGAAAAATCAAGAAGCTGAGATCGATTCTTGGATCAGGCTGGCAGGTACG
GGCTTCCGTGGGACATATCAGACAGCTCGCCAATACGGGTACGGGTAACTTAGGATTTGAAGCAAAAAATGGTCAGATCA
CCTGTAGCTATGAACCCAGGGATGACCGGGCCAAAAAGACCATCGCTGATCTGAAAACCGCCGCTAAACAGGCCGACGAG
ATTTTTATTGCCAGTGACCCAGACCGGGAAGGAGAAGTGATTGGGTGGCATATTGCCCAGGTACTTGGGATTAAGAAACC
AAAGCGGGTGGTCTATCAAGAAATTACGGAACGAGCGGTTCAGCAAGCTATTGCCCATCCCAAATCTTTAGATATGGACT
TGGTGGAAGCGGGGCGCTGTCGAGATTGCCTCGACAAACTCGTGGGCTATCGGGGGTCTCCCTTGATCTGGCGGTTGAAC
AATGGTGCAAAGTCCGTCGGTAGAGTCCAATCGGCAACCCTGCACCTGATTTGTCAGCGCGAACGCGAGATCCAAGTTTT
TGTGCCTCAGGACTATTGGTCGGTATTTGTGGAATATTCCGAAGGGTTCAAAGCGTTTTATCACGGCAGCATGACTGGGA
ACGAGCCAGACGAAACCACCATTGAGGATGCGGGGTCAGAAGCTAAAACGGAAGAATCGACCCGAGTCACCTCCCAGGCT
GCAGCCGATGCCTTGGTTGAAATAGCCCAAGCTCATCCCCACCACATCCTCTCTATCGAAGGGAAGATCACCCCCAAAAA
GCCCCCTGCCCCCTTTACCACTTCAGCTCTTCAGCAAGCTGCTGGGTCGCGCCTTAAGTTCAGCCCTGAGCAAACCATGC
AGGTGGCCCAAAAACTCTATGAAACGGGTCTGATCACCTATATGCGCACGGATTCAGTGATCTTATCCCCGGACTACTGT
GCTGCCGCTAAAAAATGGCTAGAGCAAAATGATGCGGATAATGTTCCCAAAAAAGTCGCCAAGCAGAAAGGGGGCAAGCA
AACTCAGGAAGGTCATGAGGCCATCCGCCCGACGGATATTTTCAAACCCTCTGCAGAACTCAAGCAATTCCTACCGGAAG
AGCAGTTCAAGCTATATGTGTTGATTTGGACCCGGGCCTTAGCCTCCCAGTGCAAACCCGCTCAGATCCGTAAAACCATC
GTCATTAGTCAGTCTGGTGAAGTTCAGTGGAAAGCCAAAGGCAATCTTGTAGAGTTCAAGGGTTACTCAAAATACTGGAA
GGACATTAAAGCCGATACAGAACTGCCCACCCTGCAGCCCCAACAGTCTCTCACCCTAGCCCAAGCCGATTCCCAGCAGA
AGCAAACCCAGCCTCCTGCTCGCTACAGTGAACCAAAGCTGGTGCAACTGATGGAGAAAAAGGGCATTGGTCGACCGTCT
ACCTATGCTCCCACGATCAAGACTATCAAGGCTCGGGGCTATGTCGATCTGATCAAGGGTAAACTCCAGCCCACCCAGAT
CGGCATGGAGGTGGATCAATTTATGGGGGGTGCTTTACCGGAGCTATTGGAGGCTGAATTTACGGCCCAGATGGAGAGTT
CCTTGGATGCGATCGCACAAGGGAATCAGGAGTGGCAAAACTATCTGATCGACTGGAACCGCAGCTATTTTGCCCCGGCC
TTGGCGAAGGCCTTGAAAGAGTTACCTGAACCGACTGGCAACTACGGAGGCAAAGTCTTACAAAAATCTAGAACCAAATG
CCCAGGTTGCAGTCACCCCATGTCCAAGGTGCCCAGCAAAAAGGTGAAGAAGAAGTATTTCCTCAAATGCGAGAACGGCT
GCACGGCGGACAATGGTCGAGGTCTAGTGATGTTCTGGTCCGATCGAGATAAAAATTGGCAAGTTCCCCAGGCCAAATCG
GATCAACCTACGCCGCCAGCAGAACTCACGGATTATCCCTGTCCGGCTTGTCAGCAGAAAATGGAGATTTACTCATACCA
CAAGGATGGGCAGACCAAGCAGATGCTCCGATGCTCAGACCCAAAAGCTAGATCTGATAAGAAGCATAAGAATGCGGTCT
ATTTTCAAAGTAAAGGACGGTGGTGGAGTCCTAAATATGGGGAATTGGAATAA

Upstream 100 bases:

>100_bases
CTAACCTTAGCCTCACCACAGCTACCGCAAGCCTCACCACAGCTACCGCAAGCCTCACCACAGCTACCGCAGTCATTCAC
GCATCAATAGAGAACCCAAT

Downstream 100 bases:

>100_bases
AAACGAAGAAATCCCCTGCAAAAAATCACAGGGGAAGTAAGGCTTTTGTAACTTTAGTACTTCCTCTTATAATGCCCATC
GGGAAGCGGGTTTCAAGCTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSRLLIVESPGKIKKLRSILGSGWQVRASVGHIRQLANTGTGNLGFEAKNGQITCSYEPRDDRAKKTIADLKTAAKQADE
IFIASDPDREGEVIGWHIAQVLGIKKPKRVVYQEITERAVQQAIAHPKSLDMDLVEAGRCRDCLDKLVGYRGSPLIWRLN
NGAKSVGRVQSATLHLICQREREIQVFVPQDYWSVFVEYSEGFKAFYHGSMTGNEPDETTIEDAGSEAKTEESTRVTSQA
AADALVEIAQAHPHHILSIEGKITPKKPPAPFTTSALQQAAGSRLKFSPEQTMQVAQKLYETGLITYMRTDSVILSPDYC
AAAKKWLEQNDADNVPKKVAKQKGGKQTQEGHEAIRPTDIFKPSAELKQFLPEEQFKLYVLIWTRALASQCKPAQIRKTI
VISQSGEVQWKAKGNLVEFKGYSKYWKDIKADTELPTLQPQQSLTLAQADSQQKQTQPPARYSEPKLVQLMEKKGIGRPS
TYAPTIKTIKARGYVDLIKGKLQPTQIGMEVDQFMGGALPELLEAEFTAQMESSLDAIAQGNQEWQNYLIDWNRSYFAPA
LAKALKELPEPTGNYGGKVLQKSRTKCPGCSHPMSKVPSKKVKKKYFLKCENGCTADNGRGLVMFWSDRDKNWQVPQAKS
DQPTPPAELTDYPCPACQQKMEIYSYHKDGQTKQMLRCSDPKARSDKKHKNAVYFQSKGRWWSPKYGELE

Sequences:

>Translated_710_residues
MSRLLIVESPGKIKKLRSILGSGWQVRASVGHIRQLANTGTGNLGFEAKNGQITCSYEPRDDRAKKTIADLKTAAKQADE
IFIASDPDREGEVIGWHIAQVLGIKKPKRVVYQEITERAVQQAIAHPKSLDMDLVEAGRCRDCLDKLVGYRGSPLIWRLN
NGAKSVGRVQSATLHLICQREREIQVFVPQDYWSVFVEYSEGFKAFYHGSMTGNEPDETTIEDAGSEAKTEESTRVTSQA
AADALVEIAQAHPHHILSIEGKITPKKPPAPFTTSALQQAAGSRLKFSPEQTMQVAQKLYETGLITYMRTDSVILSPDYC
AAAKKWLEQNDADNVPKKVAKQKGGKQTQEGHEAIRPTDIFKPSAELKQFLPEEQFKLYVLIWTRALASQCKPAQIRKTI
VISQSGEVQWKAKGNLVEFKGYSKYWKDIKADTELPTLQPQQSLTLAQADSQQKQTQPPARYSEPKLVQLMEKKGIGRPS
TYAPTIKTIKARGYVDLIKGKLQPTQIGMEVDQFMGGALPELLEAEFTAQMESSLDAIAQGNQEWQNYLIDWNRSYFAPA
LAKALKELPEPTGNYGGKVLQKSRTKCPGCSHPMSKVPSKKVKKKYFLKCENGCTADNGRGLVMFWSDRDKNWQVPQAKS
DQPTPPAELTDYPCPACQQKMEIYSYHKDGQTKQMLRCSDPKARSDKKHKNAVYFQSKGRWWSPKYGELE
>Mature_709_residues
SRLLIVESPGKIKKLRSILGSGWQVRASVGHIRQLANTGTGNLGFEAKNGQITCSYEPRDDRAKKTIADLKTAAKQADEI
FIASDPDREGEVIGWHIAQVLGIKKPKRVVYQEITERAVQQAIAHPKSLDMDLVEAGRCRDCLDKLVGYRGSPLIWRLNN
GAKSVGRVQSATLHLICQREREIQVFVPQDYWSVFVEYSEGFKAFYHGSMTGNEPDETTIEDAGSEAKTEESTRVTSQAA
ADALVEIAQAHPHHILSIEGKITPKKPPAPFTTSALQQAAGSRLKFSPEQTMQVAQKLYETGLITYMRTDSVILSPDYCA
AAKKWLEQNDADNVPKKVAKQKGGKQTQEGHEAIRPTDIFKPSAELKQFLPEEQFKLYVLIWTRALASQCKPAQIRKTIV
ISQSGEVQWKAKGNLVEFKGYSKYWKDIKADTELPTLQPQQSLTLAQADSQQKQTQPPARYSEPKLVQLMEKKGIGRPST
YAPTIKTIKARGYVDLIKGKLQPTQIGMEVDQFMGGALPELLEAEFTAQMESSLDAIAQGNQEWQNYLIDWNRSYFAPAL
AKALKELPEPTGNYGGKVLQKSRTKCPGCSHPMSKVPSKKVKKKYFLKCENGCTADNGRGLVMFWSDRDKNWQVPQAKSD
QPTPPAELTDYPCPACQQKMEIYSYHKDGQTKQMLRCSDPKARSDKKHKNAVYFQSKGRWWSPKYGELE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=578, Percent_Identity=25.9515570934256, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI4507635, Length=503, Percent_Identity=22.6640159045726, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1787529, Length=637, Percent_Identity=35.9497645211931, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788061, Length=618, Percent_Identity=24.4336569579288, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=508, Percent_Identity=27.755905511811, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=566, Percent_Identity=24.2049469964664, Blast_Score=126, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=550, Percent_Identity=25.2727272727273, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24585251, Length=579, Percent_Identity=25.0431778929188, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24640096, Length=525, Percent_Identity=23.4285714285714, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79561; Mature: 79429

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLIVESPGKIKKLRSILGSGWQVRASVGHIRQLANTGTGNLGFEAKNGQITCSYEPR
CCCEEEEECCCHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCC
DDRAKKTIADLKTAAKQADEIFIASDPDREGEVIGWHIAQVLGIKKPKRVVYQEITERAV
CHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHH
QQAIAHPKSLDMDLVEAGRCRDCLDKLVGYRGSPLIWRLNNGAKSVGRVQSATLHLICQR
HHHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEC
EREIQVFVPQDYWSVFVEYSEGFKAFYHGSMTGNEPDETTIEDAGSEAKTEESTRVTSQA
CCCEEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
AADALVEIAQAHPHHILSIEGKITPKKPPAPFTTSALQQAAGSRLKFSPEQTMQVAQKLY
HHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHH
ETGLITYMRTDSVILSPDYCAAAKKWLEQNDADNVPKKVAKQKGGKQTQEGHEAIRPTDI
HCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCC
FKPSAELKQFLPEEQFKLYVLIWTRALASQCKPAQIRKTIVISQSGEVQWKAKGNLVEFK
CCCCHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCHHEEEEEEECCCCEEEEECCCEEEEC
GYSKYWKDIKADTELPTLQPQQSLTLAQADSQQKQTQPPARYSEPKLVQLMEKKGIGRPS
CHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
TYAPTIKTIKARGYVDLIKGKLQPTQIGMEVDQFMGGALPELLEAEFTAQMESSLDAIAQ
CCCCHHHHHHHCCHHHHHHCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GNQEWQNYLIDWNRSYFAPALAKALKELPEPTGNYGGKVLQKSRTKCPGCSHPMSKVPSK
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCHH
KVKKKYFLKCENGCTADNGRGLVMFWSDRDKNWQVPQAKSDQPTPPAELTDYPCPACQQK
HHHHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MEIYSYHKDGQTKQMLRCSDPKARSDKKHKNAVYFQSKGRWWSPKYGELE
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLLIVESPGKIKKLRSILGSGWQVRASVGHIRQLANTGTGNLGFEAKNGQITCSYEPR
CCEEEEECCCHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCC
DDRAKKTIADLKTAAKQADEIFIASDPDREGEVIGWHIAQVLGIKKPKRVVYQEITERAV
CHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHH
QQAIAHPKSLDMDLVEAGRCRDCLDKLVGYRGSPLIWRLNNGAKSVGRVQSATLHLICQR
HHHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEC
EREIQVFVPQDYWSVFVEYSEGFKAFYHGSMTGNEPDETTIEDAGSEAKTEESTRVTSQA
CCCEEEEECCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
AADALVEIAQAHPHHILSIEGKITPKKPPAPFTTSALQQAAGSRLKFSPEQTMQVAQKLY
HHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHH
ETGLITYMRTDSVILSPDYCAAAKKWLEQNDADNVPKKVAKQKGGKQTQEGHEAIRPTDI
HCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCC
FKPSAELKQFLPEEQFKLYVLIWTRALASQCKPAQIRKTIVISQSGEVQWKAKGNLVEFK
CCCCHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCHHEEEEEEECCCCEEEEECCCEEEEC
GYSKYWKDIKADTELPTLQPQQSLTLAQADSQQKQTQPPARYSEPKLVQLMEKKGIGRPS
CHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
TYAPTIKTIKARGYVDLIKGKLQPTQIGMEVDQFMGGALPELLEAEFTAQMESSLDAIAQ
CCCCHHHHHHHCCHHHHHHCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GNQEWQNYLIDWNRSYFAPALAKALKELPEPTGNYGGKVLQKSRTKCPGCSHPMSKVPSK
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCHH
KVKKKYFLKCENGCTADNGRGLVMFWSDRDKNWQVPQAKSDQPTPPAELTDYPCPACQQK
HHHHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MEIYSYHKDGQTKQMLRCSDPKARSDKKHKNAVYFQSKGRWWSPKYGELE
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]