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The map label for this gene is glmU

Identifier: 158337981

GI number: 158337981

Start: 4920709

End: 4922076

Strand: Reverse

Name: glmU

Synonym: AM1_4868

Alternate gene names: 158337981

Gene position: 4922076-4920709 (Counterclockwise)

Preceding gene: 158337986

Following gene: 158337980

Centisome position: 75.68

GC content: 47.37

Gene sequence:

>1368_bases
ATGATCGCGGTAGCAATTTTGGCAGCGGGTAAAGGCACACGTATGAGGTCAAACCTGCCCAAGGTACTACATCAGTTAGG
GGGTAAGAGCCTGATTGAGCGAGTATTAAGCAGCACCCAAAACCTTTCTCCATCCCGTCAGATTGTGATTGTGGGCTATT
CCGCTGATCAAGTGCGGACAGAAATGCAAGGCTGGCCGAACTTAGAATTTGTGGAACAAACGGAGCAATTGGGCACAGGC
CACGCCGTCCAGCAAGTACTGCCTGTGATGGAAGGCTTTGAAGGAGACTTGCTCGTCCTCAATGGCGATGTCCCCTTACT
CCGACCTGGGACGTTGCAAAAAATGTTGGCAACCCATCAAGAGCATCAGAATGCAGCGACCATTTTGACAGCCCAATTGC
CCAATCCCAAAGGTTATGGTCGGGTCTTTTGCGATGCTCAAATGCATTTGCAAGAAATTATTGAAGATCGAGACTGTACC
CCTGCCCAACGTCAAAATCAACGCATCAATGCAGGGGTCTATTGTTTTCGCTGGTCGGCTTTAGCCAAAGTATTACCAAA
CCTGAAAGCTGAAAATGATCAAAAAGAATATTATCTGACCGATGCAGTTAACTACTTAGACCCAGTTATGGTTCTGGATG
TCGATGATTATCAAGAAATTCTAGGCATTAACGATCGCAAACAATTAGCCACCGCCTACAAGATTTTGCAAGATCGGATT
AAGGATGATTGGCTGGTTGCTGGCGTGACAATTATGGATCCAGACAGCATTACCATAGATGAAACCGTTGAATTGGGGAC
CGATGTCATTATCGAGCCTCAAACCCACCTACGGGGCGATACCAAAATTGATACCGGTAGCCGTATCGGTCCTGGGAGTT
TGATTGAGAATAGCCATATTGGCACCAATGTGCAGGTTCTTTATTCTGTTATCTCGGACAGCCGAGTGGGCGACAACTCC
CGGATAGGGCCTTATACGCATCTACGAGGGAATGTGCAGATTGGTGAGAAATGCCGGGTCGGTAATTTCGTTGAAATGAA
AAAAACGACCATTGGAGATCGCACCAACGTCGCCCATCTGTCTTATCTAGGGGATGCCACTTTAGGAACCCAGGTCAATA
TTGGTGCAGGAACCATTACTGCCAATTATGACGGGGTGAATAAACATCCAACTCAGATTGGCGATCGCACCAAAACAGGT
GCAAACAGCGTCTTAGTGGCTCCGATTACCTTAGGGGCAAATGTTACCGTGGCGGCAGGCTCCACAATTACTAAGAATGT
CGATGATGATGTCCTAGTCGTGGCGCGTCAACGGCAAAGTGTATTACCTGGATGGCGACCTATTTCCTCTAAACAGACAG
AAAAATGA

Upstream 100 bases:

>100_bases
CTTGTCTCAACCCACACTCTATCTTTAACAACAATCTGTTGTCTCTATTGGCGAAGAAGCACGATATACTCCGTGAGTGT
TGACTCCCTAGGACAAGGAC

Downstream 100 bases:

>100_bases
TGCGTTAGTCCACAACTTACAGGGCACTCTAAGCTGGGGCAGATTGATTTTTCTCAGAAAATGTCGATTTTTTAGCTGAA
TTCCCCTTATGACCTGTTCT

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIAVAILAAGKGTRMRSNLPKVLHQLGGKSLIERVLSSTQNLSPSRQIVIVGYSADQVRTEMQGWPNLEFVEQTEQLGTG
HAVQQVLPVMEGFEGDLLVLNGDVPLLRPGTLQKMLATHQEHQNAATILTAQLPNPKGYGRVFCDAQMHLQEIIEDRDCT
PAQRQNQRINAGVYCFRWSALAKVLPNLKAENDQKEYYLTDAVNYLDPVMVLDVDDYQEILGINDRKQLATAYKILQDRI
KDDWLVAGVTIMDPDSITIDETVELGTDVIIEPQTHLRGDTKIDTGSRIGPGSLIENSHIGTNVQVLYSVISDSRVGDNS
RIGPYTHLRGNVQIGEKCRVGNFVEMKKTTIGDRTNVAHLSYLGDATLGTQVNIGAGTITANYDGVNKHPTQIGDRTKTG
ANSVLVAPITLGANVTVAAGSTITKNVDDDVLVVARQRQSVLPGWRPISSKQTEK

Sequences:

>Translated_455_residues
MIAVAILAAGKGTRMRSNLPKVLHQLGGKSLIERVLSSTQNLSPSRQIVIVGYSADQVRTEMQGWPNLEFVEQTEQLGTG
HAVQQVLPVMEGFEGDLLVLNGDVPLLRPGTLQKMLATHQEHQNAATILTAQLPNPKGYGRVFCDAQMHLQEIIEDRDCT
PAQRQNQRINAGVYCFRWSALAKVLPNLKAENDQKEYYLTDAVNYLDPVMVLDVDDYQEILGINDRKQLATAYKILQDRI
KDDWLVAGVTIMDPDSITIDETVELGTDVIIEPQTHLRGDTKIDTGSRIGPGSLIENSHIGTNVQVLYSVISDSRVGDNS
RIGPYTHLRGNVQIGEKCRVGNFVEMKKTTIGDRTNVAHLSYLGDATLGTQVNIGAGTITANYDGVNKHPTQIGDRTKTG
ANSVLVAPITLGANVTVAAGSTITKNVDDDVLVVARQRQSVLPGWRPISSKQTEK
>Mature_455_residues
MIAVAILAAGKGTRMRSNLPKVLHQLGGKSLIERVLSSTQNLSPSRQIVIVGYSADQVRTEMQGWPNLEFVEQTEQLGTG
HAVQQVLPVMEGFEGDLLVLNGDVPLLRPGTLQKMLATHQEHQNAATILTAQLPNPKGYGRVFCDAQMHLQEIIEDRDCT
PAQRQNQRINAGVYCFRWSALAKVLPNLKAENDQKEYYLTDAVNYLDPVMVLDVDDYQEILGINDRKQLATAYKILQDRI
KDDWLVAGVTIMDPDSITIDETVELGTDVIIEPQTHLRGDTKIDTGSRIGPGSLIENSHIGTNVQVLYSVISDSRVGDNS
RIGPYTHLRGNVQIGEKCRVGNFVEMKKTTIGDRTNVAHLSYLGDATLGTQVNIGAGTITANYDGVNKHPTQIGDRTKTG
ANSVLVAPITLGANVTVAAGSTITKNVDDDVLVVARQRQSVLPGWRPISSKQTEK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:
GO:0000287: Bifunctional protein glmU
GO:0000902: Bifunctional protein glmU
GO:0003824: Bifunctional protein glmU
GO:0003977: Bifunctional protein glmU
GO:0005737: Bifunctional protein glmU
GO:0007047: Bifunctional protein glmU
GO:0008152: Bifunctional protein glmU
GO:0008360: Bifunctional protein glmU
GO:0009103: Bifunctional protein glmU
GO:0009252: Bifunctional protein glmU
GO:0016740: Bifunctional protein glmU
GO:0016746: Bifunctional protein glmU
GO:0016779: Bifunctional protein glmU
GO:0019134: Bifunctional protein glmU
GO:0046872: Bifunctional protein glmU

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=456, Percent_Identity=39.2543859649123, Blast_Score=311, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI133931050, Length=383, Percent_Identity=25.065274151436, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=361, Percent_Identity=24.0997229916898, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355443, Length=342, Percent_Identity=22.5146198830409, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644084, Length=342, Percent_Identity=22.5146198830409, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_ACAM1 (B0C3K5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519157.1
- ProteinModelPortal:   B0C3K5
- SMR:   B0C3K5
- GeneID:   5683668
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4868
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14360
- BioCyc:   AMAR329726:AM1_4868-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49762; Mature: 49762

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 359-359 BINDING 73-73 BINDING 140-140 BINDING 155-155 BINDING 170-170 BINDING 383-383 BINDING 419-419 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVAILAAGKGTRMRSNLPKVLHQLGGKSLIERVLSSTQNLSPSRQIVIVGYSADQVRT
CEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
EMQGWPNLEFVEQTEQLGTGHAVQQVLPVMEGFEGDLLVLNGDVPLLRPGTLQKMLATHQ
HHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
EHQNAATILTAQLPNPKGYGRVFCDAQMHLQEIIEDRDCTPAQRQNQRINAGVYCFRWSA
HCCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEHHH
LAKVLPNLKAENDQKEYYLTDAVNYLDPVMVLDVDDYQEILGINDRKQLATAYKILQDRI
HHHHHCCCCCCCCCCEEEEHHHHHHHCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHC
KDDWLVAGVTIMDPDSITIDETVELGTDVIIEPQTHLRGDTKIDTGSRIGPGSLIENSHI
CCCEEEEEEEEECCCCEEECCHHHCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCC
GTNVQVLYSVISDSRVGDNSRIGPYTHLRGNVQIGEKCRVGNFVEMKKTTIGDRTNVAHL
CCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEECCCCCCCCEEEHHHHCCCCCCCEEEE
SYLGDATLGTQVNIGAGTITANYDGVNKHPTQIGDRTKTGANSVLVAPITLGANVTVAAG
EECCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECC
STITKNVDDDVLVVARQRQSVLPGWRPISSKQTEK
CEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIAVAILAAGKGTRMRSNLPKVLHQLGGKSLIERVLSSTQNLSPSRQIVIVGYSADQVRT
CEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
EMQGWPNLEFVEQTEQLGTGHAVQQVLPVMEGFEGDLLVLNGDVPLLRPGTLQKMLATHQ
HHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
EHQNAATILTAQLPNPKGYGRVFCDAQMHLQEIIEDRDCTPAQRQNQRINAGVYCFRWSA
HCCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEHHH
LAKVLPNLKAENDQKEYYLTDAVNYLDPVMVLDVDDYQEILGINDRKQLATAYKILQDRI
HHHHHCCCCCCCCCCEEEEHHHHHHHCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHC
KDDWLVAGVTIMDPDSITIDETVELGTDVIIEPQTHLRGDTKIDTGSRIGPGSLIENSHI
CCCEEEEEEEEECCCCEEECCHHHCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCC
GTNVQVLYSVISDSRVGDNSRIGPYTHLRGNVQIGEKCRVGNFVEMKKTTIGDRTNVAHL
CCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEECCCCCCCCEEEHHHHCCCCCCCEEEE
SYLGDATLGTQVNIGAGTITANYDGVNKHPTQIGDRTKTGANSVLVAPITLGANVTVAAG
EECCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECC
STITKNVDDDVLVVARQRQSVLPGWRPISSKQTEK
CEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA