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The map label for this gene is queA

Identifier: 158337753

GI number: 158337753

Start: 4670654

End: 4671763

Strand: Reverse

Name: queA

Synonym: AM1_4637

Alternate gene names: 158337753

Gene position: 4671763-4670654 (Counterclockwise)

Preceding gene: 158337757

Following gene: 158337747

Centisome position: 71.83

GC content: 52.61

Gene sequence:

>1110_bases
ATGCTGGGCCCCACTCCCGAGTTAGATCAAACCCTCCAGGCCTATGATTATCTGCTGCCAGAGACCTTCATTGCGCAAAC
TCCTTTGGTGCCTAGAGACCGATCTCGGCTATTGGTAGTTCAACAACAAGCGCATCAGCATAAAATCTTCCAGGATTTAG
TCGCGCTACTGCAGCCTGGCGATTTGCTGGTTTTTAATGATACCCGGGTTATTCCAGCCCGTCTCCTGGGTCGCAAATTG
AATTCTGAGCTGGCCCAGCCAGTTGAGGTCTTTTTGCTGGAACCTCGACAGCCGAATACTTGGCTTGCTCTAGTCCGACC
AGGACGGCGGTTAAAGCCGGGGGCCCAGATAGAGTTTGGCCCCCCAGAACGTCCCTTATTGCGGGCAGAGATTCTGGCTA
CTGATGCAGACACAAGGGGGCGAATTATTCAGTTTGAGTCTCTGGTTGAAGAGCCGTTTGAGGATCTTCTGGAACAACTG
GGTGAAGTCCCCTTACCTCCCTATATTCAAGAGTCTACTGCCCAGCCTGAACAATACCAAACGGTTTATGCTCAACATCC
CGGGGCCGTTGCTGCCCCTACGGCTGGGTTGCATTTTACCCCTGAGTTACTCGCCCGTCTCCAATCTGAAGGTATTAATC
AGGCGCATCTGACGCTTCATGTGGGGATTGGGACGTTTCGGCCTGTGGAAACAGATGATATTTTGCAACACCATATGCAC
TCGGAATGGGTAGATTTGCCGGCAGCAACGGTAGACGCTATTCGGACAACTAAGGCCAGTGGGGGGCGCGTTATTGCGGT
GGGAACGACTGTAACGAGGGCTCTGGAGGGCGCTTGTCAGAATGGCCCGCTGGTTCCTTGGCAGGGGCGTACCAATTTAT
TTATCTATCCCAGTTACCAATGGCGGGTGGTGGATGGTTTGATCACCAACTTTCATTTGCCGAAATCGAGCTTGCTGATG
TTGGTGAGTGCGCTAGTGGGACGGGAGCGGTTATTAGCCTTGTATGAGGATGCGATCGCACAACAATACCGCTTCTATTC
CTTCGGTGACGCCATGCTGGTTTTACCGGAGGCCGTGATTGCAGAACGTTCCCCAGACCCTGTTTTCTAG

Upstream 100 bases:

>100_bases
ATGGGTAGAGCAGAATCCCCATAATTAATAGGGGGTTATACCCAACCTGGTTGATCATTGCAGTCAGGGCAATCAATTCA
CCGTCAGTAACAAAGAAAGC

Downstream 100 bases:

>100_bases
CCCCGTCCGATTAGCGGTAAACTTCGACCTTGGTTTGCTGGAATATCTGAGCGTAGAACTTTTCCAATTGATCCAGGTTT
CGATCTAGGCAATATCGTTC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLGPTPELDQTLQAYDYLLPETFIAQTPLVPRDRSRLLVVQQQAHQHKIFQDLVALLQPGDLLVFNDTRVIPARLLGRKL
NSELAQPVEVFLLEPRQPNTWLALVRPGRRLKPGAQIEFGPPERPLLRAEILATDADTRGRIIQFESLVEEPFEDLLEQL
GEVPLPPYIQESTAQPEQYQTVYAQHPGAVAAPTAGLHFTPELLARLQSEGINQAHLTLHVGIGTFRPVETDDILQHHMH
SEWVDLPAATVDAIRTTKASGGRVIAVGTTVTRALEGACQNGPLVPWQGRTNLFIYPSYQWRVVDGLITNFHLPKSSLLM
LVSALVGRERLLALYEDAIAQQYRFYSFGDAMLVLPEAVIAERSPDPVF

Sequences:

>Translated_369_residues
MLGPTPELDQTLQAYDYLLPETFIAQTPLVPRDRSRLLVVQQQAHQHKIFQDLVALLQPGDLLVFNDTRVIPARLLGRKL
NSELAQPVEVFLLEPRQPNTWLALVRPGRRLKPGAQIEFGPPERPLLRAEILATDADTRGRIIQFESLVEEPFEDLLEQL
GEVPLPPYIQESTAQPEQYQTVYAQHPGAVAAPTAGLHFTPELLARLQSEGINQAHLTLHVGIGTFRPVETDDILQHHMH
SEWVDLPAATVDAIRTTKASGGRVIAVGTTVTRALEGACQNGPLVPWQGRTNLFIYPSYQWRVVDGLITNFHLPKSSLLM
LVSALVGRERLLALYEDAIAQQYRFYSFGDAMLVLPEAVIAERSPDPVF
>Mature_369_residues
MLGPTPELDQTLQAYDYLLPETFIAQTPLVPRDRSRLLVVQQQAHQHKIFQDLVALLQPGDLLVFNDTRVIPARLLGRKL
NSELAQPVEVFLLEPRQPNTWLALVRPGRRLKPGAQIEFGPPERPLLRAEILATDADTRGRIIQFESLVEEPFEDLLEQL
GEVPLPPYIQESTAQPEQYQTVYAQHPGAVAAPTAGLHFTPELLARLQSEGINQAHLTLHVGIGTFRPVETDDILQHHMH
SEWVDLPAATVDAIRTTKASGGRVIAVGTTVTRALEGACQNGPLVPWQGRTNLFIYPSYQWRVVDGLITNFHLPKSSLLM
LVSALVGRERLLALYEDAIAQQYRFYSFGDAMLVLPEAVIAERSPDPVF

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:
GO:0005737: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
GO:0008616: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
GO:0016740: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
GO:0016853: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=45.4802259887006, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ACAM1 (B0C0B9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518929.1
- ProteinModelPortal:   B0C0B9
- SMR:   B0C0B9
- GeneID:   5683439
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4637
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   AMAR329726:AM1_4637-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41193; Mature: 41193

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGPTPELDQTLQAYDYLLPETFIAQTPLVPRDRSRLLVVQQQAHQHKIFQDLVALLQPG
CCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCC
DLLVFNDTRVIPARLLGRKLNSELAQPVEVFLLEPRQPNTWLALVRPGRRLKPGAQIEFG
CEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEECC
PPERPLLRAEILATDADTRGRIIQFESLVEEPFEDLLEQLGEVPLPPYIQESTAQPEQYQ
CCCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
TVYAQHPGAVAAPTAGLHFTPELLARLQSEGINQAHLTLHVGIGTFRPVETDDILQHHMH
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
SEWVDLPAATVDAIRTTKASGGRVIAVGTTVTRALEGACQNGPLVPWQGRTNLFIYPSYQ
HHHHCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
WRVVDGLITNFHLPKSSLLMLVSALVGRERLLALYEDAIAQQYRFYSFGDAMLVLPEAVI
EEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHHHH
AERSPDPVF
HCCCCCCCC
>Mature Secondary Structure
MLGPTPELDQTLQAYDYLLPETFIAQTPLVPRDRSRLLVVQQQAHQHKIFQDLVALLQPG
CCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCC
DLLVFNDTRVIPARLLGRKLNSELAQPVEVFLLEPRQPNTWLALVRPGRRLKPGAQIEFG
CEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEECC
PPERPLLRAEILATDADTRGRIIQFESLVEEPFEDLLEQLGEVPLPPYIQESTAQPEQYQ
CCCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
TVYAQHPGAVAAPTAGLHFTPELLARLQSEGINQAHLTLHVGIGTFRPVETDDILQHHMH
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
SEWVDLPAATVDAIRTTKASGGRVIAVGTTVTRALEGACQNGPLVPWQGRTNLFIYPSYQ
HHHHCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
WRVVDGLITNFHLPKSSLLMLVSALVGRERLLALYEDAIAQQYRFYSFGDAMLVLPEAVI
EEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHHHH
AERSPDPVF
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA